Table 1.

Escherichia coli K-12 strains used in this study

StrainRelevant genotypeReference or source
BW7261Hfr(PO2A) leu-63::Tn10 fhuA22 Δ(argF-lac)169 ompF627 relA1 spoT1CGSC#6787
RW1448arnhB+rnhA+uvrA+RW698c x BW7261
RW1450aΔrnhB782RW970c x BW7261
RW1632arnhA339::catRW1044c x BW7261
EC10713aΔuvrA753::KanRW1448 x RW1634c
EC10714aΔrnhB782 ΔuvrA753::KanRW1450 x RW1634c
RW1636aΔrnhB782::Kan rnhA339::catRW1092c x BW7261
EC10459as RW1448 but galK+RW1448 x MC4100d
EC10460as RW1450 but galK+RW1450 x MC4100d
EC10534as EC10459 but attB::lacIZYA(L)EC10459 x EC9429d
EC10535as EC10459 but attB::lacIZYA(R)EC10459 x EC9428d
EC10536as EC10460 but attB::lacIZYA(L)EC10460 x EC9429d
EC10537as EC10460 but attB::lacIZYA(R)EC10460 x EC9428d
EC9882as RW1448 but attB::lacIZYA(CC105)LRW1448 x EC3138e
EC9883as RW1448 but attB::lacIZYA(CC105)RRW1448 x EC3144e
EC9886as RW1450 but attB::lacIZYA(CC105)LRW1450 x EC3138e
EC9887as RW1450 but attB::lacIZYA(CC105)RRW1450 x EC3144e
EC10212as RW1632 but attB::lacIZYA(CC105)LRW1632 x EC3138e
EC10213as RW1632 but attB::lacIZYA(CC105)RRW1632 x EC3144e
EC10522as EC10713 but attB::lacIZYA(CC105)LEC10713 x EC3138e
EC10523as EC10713 but attB::lacIZYA(CC105)REC10713 x EC3144e
EC10524as EC10714 but attB::lacIZYA(CC105)LEC10714 x EC3138e
EC10525as EC10714 but attB::lacIZYA(CC105)REC10714 x EC3144e
EC10216as RW1636 but attB::lacIZYA(CC105)LRW1636 x EC3138e
EC10217as RW1636 but attB::lacIZYA(CC105)RRW1636 x EC3144e
RW1628bdnaE+(21)
RW1612bdnaE_S759N(21)
RW1630bdnaE+ ΔrnhB782::Kan(21)
RW1718bdnaE_S759N ΔrnhB782::Kan yafC502::Tn10(21)
RW1624bdnaE_S759T ΔrnhB782 ΔyafC727::Kan(21)
RW1736bdnaE_S759C ΔrnhB782::Kan yafC502::Tn10(21)
RW1620bdnaE+ ΔrnhB782 dnaQ920 ΔyafC727::Kan(21)
EC10545bdnaE_S759N ΔrnhB782::Kan dnaQ920 yafC502::Tn10(21)
EC10426bdnaE+ ΔrnhB782::Kan rnhA339::catRW1630 x RW1632
EC10427bdnaE_S759N ΔrnhB782::Kan yafC502::Tn10 rnhA339::catRW1630 x RW1632
StrainRelevant genotypeReference or source
BW7261Hfr(PO2A) leu-63::Tn10 fhuA22 Δ(argF-lac)169 ompF627 relA1 spoT1CGSC#6787
RW1448arnhB+rnhA+uvrA+RW698c x BW7261
RW1450aΔrnhB782RW970c x BW7261
RW1632arnhA339::catRW1044c x BW7261
EC10713aΔuvrA753::KanRW1448 x RW1634c
EC10714aΔrnhB782 ΔuvrA753::KanRW1450 x RW1634c
RW1636aΔrnhB782::Kan rnhA339::catRW1092c x BW7261
EC10459as RW1448 but galK+RW1448 x MC4100d
EC10460as RW1450 but galK+RW1450 x MC4100d
EC10534as EC10459 but attB::lacIZYA(L)EC10459 x EC9429d
EC10535as EC10459 but attB::lacIZYA(R)EC10459 x EC9428d
EC10536as EC10460 but attB::lacIZYA(L)EC10460 x EC9429d
EC10537as EC10460 but attB::lacIZYA(R)EC10460 x EC9428d
EC9882as RW1448 but attB::lacIZYA(CC105)LRW1448 x EC3138e
EC9883as RW1448 but attB::lacIZYA(CC105)RRW1448 x EC3144e
EC9886as RW1450 but attB::lacIZYA(CC105)LRW1450 x EC3138e
EC9887as RW1450 but attB::lacIZYA(CC105)RRW1450 x EC3144e
EC10212as RW1632 but attB::lacIZYA(CC105)LRW1632 x EC3138e
EC10213as RW1632 but attB::lacIZYA(CC105)RRW1632 x EC3144e
EC10522as EC10713 but attB::lacIZYA(CC105)LEC10713 x EC3138e
EC10523as EC10713 but attB::lacIZYA(CC105)REC10713 x EC3144e
EC10524as EC10714 but attB::lacIZYA(CC105)LEC10714 x EC3138e
EC10525as EC10714 but attB::lacIZYA(CC105)REC10714 x EC3144e
EC10216as RW1636 but attB::lacIZYA(CC105)LRW1636 x EC3138e
EC10217as RW1636 but attB::lacIZYA(CC105)RRW1636 x EC3144e
RW1628bdnaE+(21)
RW1612bdnaE_S759N(21)
RW1630bdnaE+ ΔrnhB782::Kan(21)
RW1718bdnaE_S759N ΔrnhB782::Kan yafC502::Tn10(21)
RW1624bdnaE_S759T ΔrnhB782 ΔyafC727::Kan(21)
RW1736bdnaE_S759C ΔrnhB782::Kan yafC502::Tn10(21)
RW1620bdnaE+ ΔrnhB782 dnaQ920 ΔyafC727::Kan(21)
EC10545bdnaE_S759N ΔrnhB782::Kan dnaQ920 yafC502::Tn10(21)
EC10426bdnaE+ ΔrnhB782::Kan rnhA339::catRW1630 x RW1632
EC10427bdnaE_S759N ΔrnhB782::Kan yafC502::Tn10 rnhA339::catRW1630 x RW1632

athr-1 araD139 Δ(argF-lac)169 tsx-33 supE44 galK2 hisG4 rpsL31 xyl-5 mtl-1 argE3 thi-1 sulA211 lexA51(Def) recA730 Δ(umuDC)596::ermGT ΔdinB61::ble.

bthr-1 Δ(argF-lac)169 tsx-33 supE44 galK2 hisG4 rpsL31 xyl-5 mtl-1 argE3 thi-1 sulA211 Δ(umuDC)596::ermGT ΔdinB61::ble ΔaraD-polB::ΩSpc.

cReferenced in (41).

dReferenced in (8).

eReferenced in (7).

Table 1.

Escherichia coli K-12 strains used in this study

StrainRelevant genotypeReference or source
BW7261Hfr(PO2A) leu-63::Tn10 fhuA22 Δ(argF-lac)169 ompF627 relA1 spoT1CGSC#6787
RW1448arnhB+rnhA+uvrA+RW698c x BW7261
RW1450aΔrnhB782RW970c x BW7261
RW1632arnhA339::catRW1044c x BW7261
EC10713aΔuvrA753::KanRW1448 x RW1634c
EC10714aΔrnhB782 ΔuvrA753::KanRW1450 x RW1634c
RW1636aΔrnhB782::Kan rnhA339::catRW1092c x BW7261
EC10459as RW1448 but galK+RW1448 x MC4100d
EC10460as RW1450 but galK+RW1450 x MC4100d
EC10534as EC10459 but attB::lacIZYA(L)EC10459 x EC9429d
EC10535as EC10459 but attB::lacIZYA(R)EC10459 x EC9428d
EC10536as EC10460 but attB::lacIZYA(L)EC10460 x EC9429d
EC10537as EC10460 but attB::lacIZYA(R)EC10460 x EC9428d
EC9882as RW1448 but attB::lacIZYA(CC105)LRW1448 x EC3138e
EC9883as RW1448 but attB::lacIZYA(CC105)RRW1448 x EC3144e
EC9886as RW1450 but attB::lacIZYA(CC105)LRW1450 x EC3138e
EC9887as RW1450 but attB::lacIZYA(CC105)RRW1450 x EC3144e
EC10212as RW1632 but attB::lacIZYA(CC105)LRW1632 x EC3138e
EC10213as RW1632 but attB::lacIZYA(CC105)RRW1632 x EC3144e
EC10522as EC10713 but attB::lacIZYA(CC105)LEC10713 x EC3138e
EC10523as EC10713 but attB::lacIZYA(CC105)REC10713 x EC3144e
EC10524as EC10714 but attB::lacIZYA(CC105)LEC10714 x EC3138e
EC10525as EC10714 but attB::lacIZYA(CC105)REC10714 x EC3144e
EC10216as RW1636 but attB::lacIZYA(CC105)LRW1636 x EC3138e
EC10217as RW1636 but attB::lacIZYA(CC105)RRW1636 x EC3144e
RW1628bdnaE+(21)
RW1612bdnaE_S759N(21)
RW1630bdnaE+ ΔrnhB782::Kan(21)
RW1718bdnaE_S759N ΔrnhB782::Kan yafC502::Tn10(21)
RW1624bdnaE_S759T ΔrnhB782 ΔyafC727::Kan(21)
RW1736bdnaE_S759C ΔrnhB782::Kan yafC502::Tn10(21)
RW1620bdnaE+ ΔrnhB782 dnaQ920 ΔyafC727::Kan(21)
EC10545bdnaE_S759N ΔrnhB782::Kan dnaQ920 yafC502::Tn10(21)
EC10426bdnaE+ ΔrnhB782::Kan rnhA339::catRW1630 x RW1632
EC10427bdnaE_S759N ΔrnhB782::Kan yafC502::Tn10 rnhA339::catRW1630 x RW1632
StrainRelevant genotypeReference or source
BW7261Hfr(PO2A) leu-63::Tn10 fhuA22 Δ(argF-lac)169 ompF627 relA1 spoT1CGSC#6787
RW1448arnhB+rnhA+uvrA+RW698c x BW7261
RW1450aΔrnhB782RW970c x BW7261
RW1632arnhA339::catRW1044c x BW7261
EC10713aΔuvrA753::KanRW1448 x RW1634c
EC10714aΔrnhB782 ΔuvrA753::KanRW1450 x RW1634c
RW1636aΔrnhB782::Kan rnhA339::catRW1092c x BW7261
EC10459as RW1448 but galK+RW1448 x MC4100d
EC10460as RW1450 but galK+RW1450 x MC4100d
EC10534as EC10459 but attB::lacIZYA(L)EC10459 x EC9429d
EC10535as EC10459 but attB::lacIZYA(R)EC10459 x EC9428d
EC10536as EC10460 but attB::lacIZYA(L)EC10460 x EC9429d
EC10537as EC10460 but attB::lacIZYA(R)EC10460 x EC9428d
EC9882as RW1448 but attB::lacIZYA(CC105)LRW1448 x EC3138e
EC9883as RW1448 but attB::lacIZYA(CC105)RRW1448 x EC3144e
EC9886as RW1450 but attB::lacIZYA(CC105)LRW1450 x EC3138e
EC9887as RW1450 but attB::lacIZYA(CC105)RRW1450 x EC3144e
EC10212as RW1632 but attB::lacIZYA(CC105)LRW1632 x EC3138e
EC10213as RW1632 but attB::lacIZYA(CC105)RRW1632 x EC3144e
EC10522as EC10713 but attB::lacIZYA(CC105)LEC10713 x EC3138e
EC10523as EC10713 but attB::lacIZYA(CC105)REC10713 x EC3144e
EC10524as EC10714 but attB::lacIZYA(CC105)LEC10714 x EC3138e
EC10525as EC10714 but attB::lacIZYA(CC105)REC10714 x EC3144e
EC10216as RW1636 but attB::lacIZYA(CC105)LRW1636 x EC3138e
EC10217as RW1636 but attB::lacIZYA(CC105)RRW1636 x EC3144e
RW1628bdnaE+(21)
RW1612bdnaE_S759N(21)
RW1630bdnaE+ ΔrnhB782::Kan(21)
RW1718bdnaE_S759N ΔrnhB782::Kan yafC502::Tn10(21)
RW1624bdnaE_S759T ΔrnhB782 ΔyafC727::Kan(21)
RW1736bdnaE_S759C ΔrnhB782::Kan yafC502::Tn10(21)
RW1620bdnaE+ ΔrnhB782 dnaQ920 ΔyafC727::Kan(21)
EC10545bdnaE_S759N ΔrnhB782::Kan dnaQ920 yafC502::Tn10(21)
EC10426bdnaE+ ΔrnhB782::Kan rnhA339::catRW1630 x RW1632
EC10427bdnaE_S759N ΔrnhB782::Kan yafC502::Tn10 rnhA339::catRW1630 x RW1632

athr-1 araD139 Δ(argF-lac)169 tsx-33 supE44 galK2 hisG4 rpsL31 xyl-5 mtl-1 argE3 thi-1 sulA211 lexA51(Def) recA730 Δ(umuDC)596::ermGT ΔdinB61::ble.

bthr-1 Δ(argF-lac)169 tsx-33 supE44 galK2 hisG4 rpsL31 xyl-5 mtl-1 argE3 thi-1 sulA211 Δ(umuDC)596::ermGT ΔdinB61::ble ΔaraD-polB::ΩSpc.

cReferenced in (41).

dReferenced in (8).

eReferenced in (7).

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