Karyotyping of mESCs adapted to N/2i condition supplemented with candidate factors
| Rank . | Growth factors . | Concentration (ng/mL) . | Karyotype (40, %) . | |||
|---|---|---|---|---|---|---|
| S2 . | S3 . | S7 . | Mean . | |||
| 1 | BMP4 | 10 | 80.00 | 82.50 | 85.00 | 82.50 |
| 2 | BMP7 | 10 | 62.50 | 67.50 | 55.00 | 61.67 |
| 3 | BMP2 | 10 | 55.00 | 47.50 | 52.50 | 51.67 |
| 4 | Tgfβ1 | 2 | 50.00 | 55.00 | 47.50 | 50.83 |
| 5 | IGF-1 | 10 | 45.00 | 47.50 | 60.00 | 50.83 |
| 6 | Activin A | 20 | 42.50 | 60.00 | 45.00 | 49.17 |
| 7 | bFGF | 10 | 52.50 | 37.50 | 45.00 | 45.00 |
| 8 | PBSa | N/A | 55.00 | 37.50 | 35.00 | 42.50 |
| 9 | EGF | 10 | 35.00 | 42.50 | 42.50 | 40.00 |
| 10 | FGF4 | 25 | 47.50 | 40.00 | 32.50 | 40.00 |
| Rank . | Growth factors . | Concentration (ng/mL) . | Karyotype (40, %) . | |||
|---|---|---|---|---|---|---|
| S2 . | S3 . | S7 . | Mean . | |||
| 1 | BMP4 | 10 | 80.00 | 82.50 | 85.00 | 82.50 |
| 2 | BMP7 | 10 | 62.50 | 67.50 | 55.00 | 61.67 |
| 3 | BMP2 | 10 | 55.00 | 47.50 | 52.50 | 51.67 |
| 4 | Tgfβ1 | 2 | 50.00 | 55.00 | 47.50 | 50.83 |
| 5 | IGF-1 | 10 | 45.00 | 47.50 | 60.00 | 50.83 |
| 6 | Activin A | 20 | 42.50 | 60.00 | 45.00 | 49.17 |
| 7 | bFGF | 10 | 52.50 | 37.50 | 45.00 | 45.00 |
| 8 | PBSa | N/A | 55.00 | 37.50 | 35.00 | 42.50 |
| 9 | EGF | 10 | 35.00 | 42.50 | 42.50 | 40.00 |
| 10 | FGF4 | 25 | 47.50 | 40.00 | 32.50 | 40.00 |
Blank control; N/A, Not Applicable.
Karyotyping of mESCs adapted to N/2i condition supplemented with candidate factors
| Rank . | Growth factors . | Concentration (ng/mL) . | Karyotype (40, %) . | |||
|---|---|---|---|---|---|---|
| S2 . | S3 . | S7 . | Mean . | |||
| 1 | BMP4 | 10 | 80.00 | 82.50 | 85.00 | 82.50 |
| 2 | BMP7 | 10 | 62.50 | 67.50 | 55.00 | 61.67 |
| 3 | BMP2 | 10 | 55.00 | 47.50 | 52.50 | 51.67 |
| 4 | Tgfβ1 | 2 | 50.00 | 55.00 | 47.50 | 50.83 |
| 5 | IGF-1 | 10 | 45.00 | 47.50 | 60.00 | 50.83 |
| 6 | Activin A | 20 | 42.50 | 60.00 | 45.00 | 49.17 |
| 7 | bFGF | 10 | 52.50 | 37.50 | 45.00 | 45.00 |
| 8 | PBSa | N/A | 55.00 | 37.50 | 35.00 | 42.50 |
| 9 | EGF | 10 | 35.00 | 42.50 | 42.50 | 40.00 |
| 10 | FGF4 | 25 | 47.50 | 40.00 | 32.50 | 40.00 |
| Rank . | Growth factors . | Concentration (ng/mL) . | Karyotype (40, %) . | |||
|---|---|---|---|---|---|---|
| S2 . | S3 . | S7 . | Mean . | |||
| 1 | BMP4 | 10 | 80.00 | 82.50 | 85.00 | 82.50 |
| 2 | BMP7 | 10 | 62.50 | 67.50 | 55.00 | 61.67 |
| 3 | BMP2 | 10 | 55.00 | 47.50 | 52.50 | 51.67 |
| 4 | Tgfβ1 | 2 | 50.00 | 55.00 | 47.50 | 50.83 |
| 5 | IGF-1 | 10 | 45.00 | 47.50 | 60.00 | 50.83 |
| 6 | Activin A | 20 | 42.50 | 60.00 | 45.00 | 49.17 |
| 7 | bFGF | 10 | 52.50 | 37.50 | 45.00 | 45.00 |
| 8 | PBSa | N/A | 55.00 | 37.50 | 35.00 | 42.50 |
| 9 | EGF | 10 | 35.00 | 42.50 | 42.50 | 40.00 |
| 10 | FGF4 | 25 | 47.50 | 40.00 | 32.50 | 40.00 |
Blank control; N/A, Not Applicable.
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