Table 1.

Results of tests and measurements assessing spatial aggregation of diploid and tetraploid plants of Centaurea stoebe and vegetation-cover densities of microsites in which these plants were recorded in three mixed-ploidy sites surveyed in the years 2009 and 2020. The numbers of flow cytometrically analysed plants for each of the two cytotypes (2x/4x) are given in parentheses following the site-year codes. Mantel test—correlation coefficient values (rm). Distance—mean pairwise distance computed separately for diploid and tetraploid plants per site and year with results of spatial aggregation test—SAT (see Materials and Methods). Vegetation density—mean vegetation-cover density of 20 × 20 cm plots around each of 2x and 4x plants (see Materials and Methods), ↑ denotes a significant increase, while ↓ denotes a significant decrease (Wilcoxon test) in vegetation-cover density per site and ploidy in 2020 when compared to 2009. Vegetation density 2x versus 4x—signed-rank sum T values (Wilcoxon test) corresponding to differences in vegetation-cover densities between 2x and 4x plants at a given site and year.

Site-yearMantel testDistance 2x (SAT)Distance 4x (SAT)Vegetation density 2xVegetation density 4xVegetation density 2x versus 4x
KOP 2009 (83/48)0.176***52.3 ± 31.829.8 ± 18.8***2.07 ± 0.752.02 ± 0.642062.5
KOP 2020 (119/41)060.9 ± 3636.7 ± 19***1.86 ± 0.72 (119)↓*1.93 ± 0.752320
SAND 2009 (193/115)0.263***17.4 ± 10.8 ***19.7 ± 11.9***2.33 ± 1.131.82 ± 0.9513910***
SAND 2020 (274/116)0.281***23.6 ± 14.1 ***20.9 ± 13***2.32 ± 0.922.08 ± 0.89↑*17294*
TLM 2009 (76/39)0.430***23.7 ± 18.3 ***24.9 ± 20.3**2.22 ± 0.762.05 ± 0.651655.5
TLM 2020 (30/43)0.478***25.3 ± 22.1 ***38.3 ± 27.3***2.60 ± 1.00↑MS2.63 ± 1.18↑*652
Site-yearMantel testDistance 2x (SAT)Distance 4x (SAT)Vegetation density 2xVegetation density 4xVegetation density 2x versus 4x
KOP 2009 (83/48)0.176***52.3 ± 31.829.8 ± 18.8***2.07 ± 0.752.02 ± 0.642062.5
KOP 2020 (119/41)060.9 ± 3636.7 ± 19***1.86 ± 0.72 (119)↓*1.93 ± 0.752320
SAND 2009 (193/115)0.263***17.4 ± 10.8 ***19.7 ± 11.9***2.33 ± 1.131.82 ± 0.9513910***
SAND 2020 (274/116)0.281***23.6 ± 14.1 ***20.9 ± 13***2.32 ± 0.922.08 ± 0.89↑*17294*
TLM 2009 (76/39)0.430***23.7 ± 18.3 ***24.9 ± 20.3**2.22 ± 0.762.05 ± 0.651655.5
TLM 2020 (30/43)0.478***25.3 ± 22.1 ***38.3 ± 27.3***2.60 ± 1.00↑MS2.63 ± 1.18↑*652

values in bold: MSP < 0.1 (marginally significant); *P < 0.05; **P < 0.01; ***P < 0.001.

Table 1.

Results of tests and measurements assessing spatial aggregation of diploid and tetraploid plants of Centaurea stoebe and vegetation-cover densities of microsites in which these plants were recorded in three mixed-ploidy sites surveyed in the years 2009 and 2020. The numbers of flow cytometrically analysed plants for each of the two cytotypes (2x/4x) are given in parentheses following the site-year codes. Mantel test—correlation coefficient values (rm). Distance—mean pairwise distance computed separately for diploid and tetraploid plants per site and year with results of spatial aggregation test—SAT (see Materials and Methods). Vegetation density—mean vegetation-cover density of 20 × 20 cm plots around each of 2x and 4x plants (see Materials and Methods), ↑ denotes a significant increase, while ↓ denotes a significant decrease (Wilcoxon test) in vegetation-cover density per site and ploidy in 2020 when compared to 2009. Vegetation density 2x versus 4x—signed-rank sum T values (Wilcoxon test) corresponding to differences in vegetation-cover densities between 2x and 4x plants at a given site and year.

Site-yearMantel testDistance 2x (SAT)Distance 4x (SAT)Vegetation density 2xVegetation density 4xVegetation density 2x versus 4x
KOP 2009 (83/48)0.176***52.3 ± 31.829.8 ± 18.8***2.07 ± 0.752.02 ± 0.642062.5
KOP 2020 (119/41)060.9 ± 3636.7 ± 19***1.86 ± 0.72 (119)↓*1.93 ± 0.752320
SAND 2009 (193/115)0.263***17.4 ± 10.8 ***19.7 ± 11.9***2.33 ± 1.131.82 ± 0.9513910***
SAND 2020 (274/116)0.281***23.6 ± 14.1 ***20.9 ± 13***2.32 ± 0.922.08 ± 0.89↑*17294*
TLM 2009 (76/39)0.430***23.7 ± 18.3 ***24.9 ± 20.3**2.22 ± 0.762.05 ± 0.651655.5
TLM 2020 (30/43)0.478***25.3 ± 22.1 ***38.3 ± 27.3***2.60 ± 1.00↑MS2.63 ± 1.18↑*652
Site-yearMantel testDistance 2x (SAT)Distance 4x (SAT)Vegetation density 2xVegetation density 4xVegetation density 2x versus 4x
KOP 2009 (83/48)0.176***52.3 ± 31.829.8 ± 18.8***2.07 ± 0.752.02 ± 0.642062.5
KOP 2020 (119/41)060.9 ± 3636.7 ± 19***1.86 ± 0.72 (119)↓*1.93 ± 0.752320
SAND 2009 (193/115)0.263***17.4 ± 10.8 ***19.7 ± 11.9***2.33 ± 1.131.82 ± 0.9513910***
SAND 2020 (274/116)0.281***23.6 ± 14.1 ***20.9 ± 13***2.32 ± 0.922.08 ± 0.89↑*17294*
TLM 2009 (76/39)0.430***23.7 ± 18.3 ***24.9 ± 20.3**2.22 ± 0.762.05 ± 0.651655.5
TLM 2020 (30/43)0.478***25.3 ± 22.1 ***38.3 ± 27.3***2.60 ± 1.00↑MS2.63 ± 1.18↑*652

values in bold: MSP < 0.1 (marginally significant); *P < 0.05; **P < 0.01; ***P < 0.001.

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