Table 2

BLAST can give ambiguous results; longer homopolymer of serine has higher e-value than shorter homopolymer of glutamine even though both homopolymers are aligned without penalty. Table shows perfect alignments collected from BLAST. It compares proteins from krueppel C2H2-type zinc-finger protein family (upper sequences) with proteins from other families (bottom sequences)

#Protein nameAlignmentse-value
1Zinc finger protein 865 (P0CJ78)SSSSSSSSSSSSSSSSSSSSSSSSS (92 - 116)1,10E-17
midlineSSSSSSSSSSSSSSSSSSSSSSSSS
ADP-ribosylation factor-like protein 6-interacting protein 4 (Q66PJ3)SSSSSSSSSSSSSSSSSSSSSSSSS (257 - 281)
2Zinc finger protein rotund (Q9VI93)QQQQQQQQQQQQQQQQQQQQQQQ (739 - 761)1,40E-23
midlineQQQQQQQQQQQQQQQQQQQQQQQ
Ataxin-2 (Q99700)QQQQQQQQQQQQQQQQQQQQQQQ (165 - 187)
3Zinc finger homeobox protein 4 (Q86UP3)PPPPPPPPPPPP (3112 - 3123)3,61E-10
midlinePPPPPPPPPPPP
Inactive histone-lysine N-methyltransferase 2E (Q3UG20)PPPPPPPPPPPP (1719 - 1730)
4Zinc finger homeobox protein 4 (Q86UP3)PPPPPPPPPPPP (3112 - 3123)1,84E-09
midlinePPPPPPPPPPPP
Translation initiation factor IF-2 (Q2G5E7)AAPTAAPAAAATPAPTPVAPPPPPPPPPPPP (53 - 83)
5Zinc finger homeobox protein 4 (Q86UP3)PPPPPPPPPPPP (3112 - 3123)7,75E-10
midlinePPPPPPPPPPPP
Glyceraldehyde-3-phosphate dehydrogenase, testis-specific (Q64467)PPPPPPPPPPPPPPPPPPPP (83 - 99)
#Protein nameAlignmentse-value
1Zinc finger protein 865 (P0CJ78)SSSSSSSSSSSSSSSSSSSSSSSSS (92 - 116)1,10E-17
midlineSSSSSSSSSSSSSSSSSSSSSSSSS
ADP-ribosylation factor-like protein 6-interacting protein 4 (Q66PJ3)SSSSSSSSSSSSSSSSSSSSSSSSS (257 - 281)
2Zinc finger protein rotund (Q9VI93)QQQQQQQQQQQQQQQQQQQQQQQ (739 - 761)1,40E-23
midlineQQQQQQQQQQQQQQQQQQQQQQQ
Ataxin-2 (Q99700)QQQQQQQQQQQQQQQQQQQQQQQ (165 - 187)
3Zinc finger homeobox protein 4 (Q86UP3)PPPPPPPPPPPP (3112 - 3123)3,61E-10
midlinePPPPPPPPPPPP
Inactive histone-lysine N-methyltransferase 2E (Q3UG20)PPPPPPPPPPPP (1719 - 1730)
4Zinc finger homeobox protein 4 (Q86UP3)PPPPPPPPPPPP (3112 - 3123)1,84E-09
midlinePPPPPPPPPPPP
Translation initiation factor IF-2 (Q2G5E7)AAPTAAPAAAATPAPTPVAPPPPPPPPPPPP (53 - 83)
5Zinc finger homeobox protein 4 (Q86UP3)PPPPPPPPPPPP (3112 - 3123)7,75E-10
midlinePPPPPPPPPPPP
Glyceraldehyde-3-phosphate dehydrogenase, testis-specific (Q64467)PPPPPPPPPPPPPPPPPPPP (83 - 99)
Table 2

BLAST can give ambiguous results; longer homopolymer of serine has higher e-value than shorter homopolymer of glutamine even though both homopolymers are aligned without penalty. Table shows perfect alignments collected from BLAST. It compares proteins from krueppel C2H2-type zinc-finger protein family (upper sequences) with proteins from other families (bottom sequences)

#Protein nameAlignmentse-value
1Zinc finger protein 865 (P0CJ78)SSSSSSSSSSSSSSSSSSSSSSSSS (92 - 116)1,10E-17
midlineSSSSSSSSSSSSSSSSSSSSSSSSS
ADP-ribosylation factor-like protein 6-interacting protein 4 (Q66PJ3)SSSSSSSSSSSSSSSSSSSSSSSSS (257 - 281)
2Zinc finger protein rotund (Q9VI93)QQQQQQQQQQQQQQQQQQQQQQQ (739 - 761)1,40E-23
midlineQQQQQQQQQQQQQQQQQQQQQQQ
Ataxin-2 (Q99700)QQQQQQQQQQQQQQQQQQQQQQQ (165 - 187)
3Zinc finger homeobox protein 4 (Q86UP3)PPPPPPPPPPPP (3112 - 3123)3,61E-10
midlinePPPPPPPPPPPP
Inactive histone-lysine N-methyltransferase 2E (Q3UG20)PPPPPPPPPPPP (1719 - 1730)
4Zinc finger homeobox protein 4 (Q86UP3)PPPPPPPPPPPP (3112 - 3123)1,84E-09
midlinePPPPPPPPPPPP
Translation initiation factor IF-2 (Q2G5E7)AAPTAAPAAAATPAPTPVAPPPPPPPPPPPP (53 - 83)
5Zinc finger homeobox protein 4 (Q86UP3)PPPPPPPPPPPP (3112 - 3123)7,75E-10
midlinePPPPPPPPPPPP
Glyceraldehyde-3-phosphate dehydrogenase, testis-specific (Q64467)PPPPPPPPPPPPPPPPPPPP (83 - 99)
#Protein nameAlignmentse-value
1Zinc finger protein 865 (P0CJ78)SSSSSSSSSSSSSSSSSSSSSSSSS (92 - 116)1,10E-17
midlineSSSSSSSSSSSSSSSSSSSSSSSSS
ADP-ribosylation factor-like protein 6-interacting protein 4 (Q66PJ3)SSSSSSSSSSSSSSSSSSSSSSSSS (257 - 281)
2Zinc finger protein rotund (Q9VI93)QQQQQQQQQQQQQQQQQQQQQQQ (739 - 761)1,40E-23
midlineQQQQQQQQQQQQQQQQQQQQQQQ
Ataxin-2 (Q99700)QQQQQQQQQQQQQQQQQQQQQQQ (165 - 187)
3Zinc finger homeobox protein 4 (Q86UP3)PPPPPPPPPPPP (3112 - 3123)3,61E-10
midlinePPPPPPPPPPPP
Inactive histone-lysine N-methyltransferase 2E (Q3UG20)PPPPPPPPPPPP (1719 - 1730)
4Zinc finger homeobox protein 4 (Q86UP3)PPPPPPPPPPPP (3112 - 3123)1,84E-09
midlinePPPPPPPPPPPP
Translation initiation factor IF-2 (Q2G5E7)AAPTAAPAAAATPAPTPVAPPPPPPPPPPPP (53 - 83)
5Zinc finger homeobox protein 4 (Q86UP3)PPPPPPPPPPPP (3112 - 3123)7,75E-10
midlinePPPPPPPPPPPP
Glyceraldehyde-3-phosphate dehydrogenase, testis-specific (Q64467)PPPPPPPPPPPPPPPPPPPP (83 - 99)
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