BLAST can give ambiguous results; longer homopolymer of serine has higher e-value than shorter homopolymer of glutamine even though both homopolymers are aligned without penalty. Table shows perfect alignments collected from BLAST. It compares proteins from krueppel C2H2-type zinc-finger protein family (upper sequences) with proteins from other families (bottom sequences)
# . | Protein name . | Alignments . | e-value . |
---|---|---|---|
1 | Zinc finger protein 865 (P0CJ78) | SSSSSSSSSSSSSSSSSSSSSSSSS (92 - 116) | 1,10E-17 |
midline | SSSSSSSSSSSSSSSSSSSSSSSSS | ||
ADP-ribosylation factor-like protein 6-interacting protein 4 (Q66PJ3) | SSSSSSSSSSSSSSSSSSSSSSSSS (257 - 281) | ||
2 | Zinc finger protein rotund (Q9VI93) | QQQQQQQQQQQQQQQQQQQQQQQ (739 - 761) | 1,40E-23 |
midline | QQQQQQQQQQQQQQQQQQQQQQQ | ||
Ataxin-2 (Q99700) | QQQQQQQQQQQQQQQQQQQQQQQ (165 - 187) | ||
3 | Zinc finger homeobox protein 4 (Q86UP3) | PPPPPPPPPPPP (3112 - 3123) | 3,61E-10 |
midline | PPPPPPPPPPPP | ||
Inactive histone-lysine N-methyltransferase 2E (Q3UG20) | PPPPPPPPPPPP (1719 - 1730) | ||
4 | Zinc finger homeobox protein 4 (Q86UP3) | PPPPPPPPPPPP (3112 - 3123) | 1,84E-09 |
midline | PPPPPPPPPPPP | ||
Translation initiation factor IF-2 (Q2G5E7) | AAPTAAPAAAATPAPTPVAPPPPPPPPPPPP (53 - 83) | ||
5 | Zinc finger homeobox protein 4 (Q86UP3) | PPPPPPPPPPPP (3112 - 3123) | 7,75E-10 |
midline | PPPPPPPPPPPP | ||
Glyceraldehyde-3-phosphate dehydrogenase, testis-specific (Q64467) | PPPPPPPPPPPPPPPPPPPP (83 - 99) |
# . | Protein name . | Alignments . | e-value . |
---|---|---|---|
1 | Zinc finger protein 865 (P0CJ78) | SSSSSSSSSSSSSSSSSSSSSSSSS (92 - 116) | 1,10E-17 |
midline | SSSSSSSSSSSSSSSSSSSSSSSSS | ||
ADP-ribosylation factor-like protein 6-interacting protein 4 (Q66PJ3) | SSSSSSSSSSSSSSSSSSSSSSSSS (257 - 281) | ||
2 | Zinc finger protein rotund (Q9VI93) | QQQQQQQQQQQQQQQQQQQQQQQ (739 - 761) | 1,40E-23 |
midline | QQQQQQQQQQQQQQQQQQQQQQQ | ||
Ataxin-2 (Q99700) | QQQQQQQQQQQQQQQQQQQQQQQ (165 - 187) | ||
3 | Zinc finger homeobox protein 4 (Q86UP3) | PPPPPPPPPPPP (3112 - 3123) | 3,61E-10 |
midline | PPPPPPPPPPPP | ||
Inactive histone-lysine N-methyltransferase 2E (Q3UG20) | PPPPPPPPPPPP (1719 - 1730) | ||
4 | Zinc finger homeobox protein 4 (Q86UP3) | PPPPPPPPPPPP (3112 - 3123) | 1,84E-09 |
midline | PPPPPPPPPPPP | ||
Translation initiation factor IF-2 (Q2G5E7) | AAPTAAPAAAATPAPTPVAPPPPPPPPPPPP (53 - 83) | ||
5 | Zinc finger homeobox protein 4 (Q86UP3) | PPPPPPPPPPPP (3112 - 3123) | 7,75E-10 |
midline | PPPPPPPPPPPP | ||
Glyceraldehyde-3-phosphate dehydrogenase, testis-specific (Q64467) | PPPPPPPPPPPPPPPPPPPP (83 - 99) |
BLAST can give ambiguous results; longer homopolymer of serine has higher e-value than shorter homopolymer of glutamine even though both homopolymers are aligned without penalty. Table shows perfect alignments collected from BLAST. It compares proteins from krueppel C2H2-type zinc-finger protein family (upper sequences) with proteins from other families (bottom sequences)
# . | Protein name . | Alignments . | e-value . |
---|---|---|---|
1 | Zinc finger protein 865 (P0CJ78) | SSSSSSSSSSSSSSSSSSSSSSSSS (92 - 116) | 1,10E-17 |
midline | SSSSSSSSSSSSSSSSSSSSSSSSS | ||
ADP-ribosylation factor-like protein 6-interacting protein 4 (Q66PJ3) | SSSSSSSSSSSSSSSSSSSSSSSSS (257 - 281) | ||
2 | Zinc finger protein rotund (Q9VI93) | QQQQQQQQQQQQQQQQQQQQQQQ (739 - 761) | 1,40E-23 |
midline | QQQQQQQQQQQQQQQQQQQQQQQ | ||
Ataxin-2 (Q99700) | QQQQQQQQQQQQQQQQQQQQQQQ (165 - 187) | ||
3 | Zinc finger homeobox protein 4 (Q86UP3) | PPPPPPPPPPPP (3112 - 3123) | 3,61E-10 |
midline | PPPPPPPPPPPP | ||
Inactive histone-lysine N-methyltransferase 2E (Q3UG20) | PPPPPPPPPPPP (1719 - 1730) | ||
4 | Zinc finger homeobox protein 4 (Q86UP3) | PPPPPPPPPPPP (3112 - 3123) | 1,84E-09 |
midline | PPPPPPPPPPPP | ||
Translation initiation factor IF-2 (Q2G5E7) | AAPTAAPAAAATPAPTPVAPPPPPPPPPPPP (53 - 83) | ||
5 | Zinc finger homeobox protein 4 (Q86UP3) | PPPPPPPPPPPP (3112 - 3123) | 7,75E-10 |
midline | PPPPPPPPPPPP | ||
Glyceraldehyde-3-phosphate dehydrogenase, testis-specific (Q64467) | PPPPPPPPPPPPPPPPPPPP (83 - 99) |
# . | Protein name . | Alignments . | e-value . |
---|---|---|---|
1 | Zinc finger protein 865 (P0CJ78) | SSSSSSSSSSSSSSSSSSSSSSSSS (92 - 116) | 1,10E-17 |
midline | SSSSSSSSSSSSSSSSSSSSSSSSS | ||
ADP-ribosylation factor-like protein 6-interacting protein 4 (Q66PJ3) | SSSSSSSSSSSSSSSSSSSSSSSSS (257 - 281) | ||
2 | Zinc finger protein rotund (Q9VI93) | QQQQQQQQQQQQQQQQQQQQQQQ (739 - 761) | 1,40E-23 |
midline | QQQQQQQQQQQQQQQQQQQQQQQ | ||
Ataxin-2 (Q99700) | QQQQQQQQQQQQQQQQQQQQQQQ (165 - 187) | ||
3 | Zinc finger homeobox protein 4 (Q86UP3) | PPPPPPPPPPPP (3112 - 3123) | 3,61E-10 |
midline | PPPPPPPPPPPP | ||
Inactive histone-lysine N-methyltransferase 2E (Q3UG20) | PPPPPPPPPPPP (1719 - 1730) | ||
4 | Zinc finger homeobox protein 4 (Q86UP3) | PPPPPPPPPPPP (3112 - 3123) | 1,84E-09 |
midline | PPPPPPPPPPPP | ||
Translation initiation factor IF-2 (Q2G5E7) | AAPTAAPAAAATPAPTPVAPPPPPPPPPPPP (53 - 83) | ||
5 | Zinc finger homeobox protein 4 (Q86UP3) | PPPPPPPPPPPP (3112 - 3123) | 7,75E-10 |
midline | PPPPPPPPPPPP | ||
Glyceraldehyde-3-phosphate dehydrogenase, testis-specific (Q64467) | PPPPPPPPPPPPPPPPPPPP (83 - 99) |
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