| Patient . | Initial CT Values (E-Gene/S-Gene/RdRP-Gene/N-Gene; VOC-Analysesa) . | Deep Sequencing . | Mutations Identified in Spike Domains . |
|---|---|---|---|
| P1 | 13/-/17/15 | B.1.389 | D614G, T723I |
| P2 | 14/-/16/17 | B.1.1.70 | D614G |
| P3 | 13/-/14/15 | B.1.1.70 | D614G |
| P4 | 16/-/17/20 | B.1.1.70 | D614G |
| P5 | 12/-/13/14 | B.1.221 | S98F, D614G |
| P6 | 17/-/18/16 | B.1.177.7 | A222V, D614G |
| P7 | 21/-/22/19 | B.1.177 | A222V, D614G, A1020V |
| P8 | 16/-/16/18 | B.1.221 | S98F, L141LF, D614G |
| P9 | -/16/16/14 | B.1.177 | A222V, D614G |
| P10 | -/12/13/13 | B.1.1.153 | D614G |
| P11 | -/24/25/23; VOC B1.351a | B.1.351 | H69Y, D80A, D215G, del242-244, K417KT, E484EK, N501NY, D614G, A701V |
| P12 | -/24/23/32; VOC B1.1.7a | -b | -b |
| P13 | -/17/16/25; VOC B1.1.7a | B.1.1.7 | del144, N501NY, A570D, D614G, P681H, A694AS, T716I, S982A, D1118H |
| P14 | -/13/12/21; VOC B1.1.7a | B.1.1.7 | del69-70, S98F, D138DH, H245Y, N501NY, A570D, D614G, P681H, A694AS, T716I, S982A, D1118DH |
| P15 | -/21/20/29; VOC B1.1.7a | B.1.1.7 | del69-70, del144, N501Y, A570D, D614G, P681H, A694AS, T716I, S982A, D1118H |
| Patient . | Initial CT Values (E-Gene/S-Gene/RdRP-Gene/N-Gene; VOC-Analysesa) . | Deep Sequencing . | Mutations Identified in Spike Domains . |
|---|---|---|---|
| P1 | 13/-/17/15 | B.1.389 | D614G, T723I |
| P2 | 14/-/16/17 | B.1.1.70 | D614G |
| P3 | 13/-/14/15 | B.1.1.70 | D614G |
| P4 | 16/-/17/20 | B.1.1.70 | D614G |
| P5 | 12/-/13/14 | B.1.221 | S98F, D614G |
| P6 | 17/-/18/16 | B.1.177.7 | A222V, D614G |
| P7 | 21/-/22/19 | B.1.177 | A222V, D614G, A1020V |
| P8 | 16/-/16/18 | B.1.221 | S98F, L141LF, D614G |
| P9 | -/16/16/14 | B.1.177 | A222V, D614G |
| P10 | -/12/13/13 | B.1.1.153 | D614G |
| P11 | -/24/25/23; VOC B1.351a | B.1.351 | H69Y, D80A, D215G, del242-244, K417KT, E484EK, N501NY, D614G, A701V |
| P12 | -/24/23/32; VOC B1.1.7a | -b | -b |
| P13 | -/17/16/25; VOC B1.1.7a | B.1.1.7 | del144, N501NY, A570D, D614G, P681H, A694AS, T716I, S982A, D1118H |
| P14 | -/13/12/21; VOC B1.1.7a | B.1.1.7 | del69-70, S98F, D138DH, H245Y, N501NY, A570D, D614G, P681H, A694AS, T716I, S982A, D1118DH |
| P15 | -/21/20/29; VOC B1.1.7a | B.1.1.7 | del69-70, del144, N501Y, A570D, D614G, P681H, A694AS, T716I, S982A, D1118H |
Abbreviations: CT, cycle threshold resulting from RT-PCR performed at St. Josef-Hospital Bochum; VOC, variants of concern.
NOTE: Data are presented as individual data. Lineage of deep sequencing is presented according to the pangolin tool [13]. Bold = characteristic mutations associated with VOC according to Robert Koch Institute [17].
VOC analyses started in the midst of the study period in February 2020 and was performed via melting curve analysis.
No sufficient material available.
| Patient . | Initial CT Values (E-Gene/S-Gene/RdRP-Gene/N-Gene; VOC-Analysesa) . | Deep Sequencing . | Mutations Identified in Spike Domains . |
|---|---|---|---|
| P1 | 13/-/17/15 | B.1.389 | D614G, T723I |
| P2 | 14/-/16/17 | B.1.1.70 | D614G |
| P3 | 13/-/14/15 | B.1.1.70 | D614G |
| P4 | 16/-/17/20 | B.1.1.70 | D614G |
| P5 | 12/-/13/14 | B.1.221 | S98F, D614G |
| P6 | 17/-/18/16 | B.1.177.7 | A222V, D614G |
| P7 | 21/-/22/19 | B.1.177 | A222V, D614G, A1020V |
| P8 | 16/-/16/18 | B.1.221 | S98F, L141LF, D614G |
| P9 | -/16/16/14 | B.1.177 | A222V, D614G |
| P10 | -/12/13/13 | B.1.1.153 | D614G |
| P11 | -/24/25/23; VOC B1.351a | B.1.351 | H69Y, D80A, D215G, del242-244, K417KT, E484EK, N501NY, D614G, A701V |
| P12 | -/24/23/32; VOC B1.1.7a | -b | -b |
| P13 | -/17/16/25; VOC B1.1.7a | B.1.1.7 | del144, N501NY, A570D, D614G, P681H, A694AS, T716I, S982A, D1118H |
| P14 | -/13/12/21; VOC B1.1.7a | B.1.1.7 | del69-70, S98F, D138DH, H245Y, N501NY, A570D, D614G, P681H, A694AS, T716I, S982A, D1118DH |
| P15 | -/21/20/29; VOC B1.1.7a | B.1.1.7 | del69-70, del144, N501Y, A570D, D614G, P681H, A694AS, T716I, S982A, D1118H |
| Patient . | Initial CT Values (E-Gene/S-Gene/RdRP-Gene/N-Gene; VOC-Analysesa) . | Deep Sequencing . | Mutations Identified in Spike Domains . |
|---|---|---|---|
| P1 | 13/-/17/15 | B.1.389 | D614G, T723I |
| P2 | 14/-/16/17 | B.1.1.70 | D614G |
| P3 | 13/-/14/15 | B.1.1.70 | D614G |
| P4 | 16/-/17/20 | B.1.1.70 | D614G |
| P5 | 12/-/13/14 | B.1.221 | S98F, D614G |
| P6 | 17/-/18/16 | B.1.177.7 | A222V, D614G |
| P7 | 21/-/22/19 | B.1.177 | A222V, D614G, A1020V |
| P8 | 16/-/16/18 | B.1.221 | S98F, L141LF, D614G |
| P9 | -/16/16/14 | B.1.177 | A222V, D614G |
| P10 | -/12/13/13 | B.1.1.153 | D614G |
| P11 | -/24/25/23; VOC B1.351a | B.1.351 | H69Y, D80A, D215G, del242-244, K417KT, E484EK, N501NY, D614G, A701V |
| P12 | -/24/23/32; VOC B1.1.7a | -b | -b |
| P13 | -/17/16/25; VOC B1.1.7a | B.1.1.7 | del144, N501NY, A570D, D614G, P681H, A694AS, T716I, S982A, D1118H |
| P14 | -/13/12/21; VOC B1.1.7a | B.1.1.7 | del69-70, S98F, D138DH, H245Y, N501NY, A570D, D614G, P681H, A694AS, T716I, S982A, D1118DH |
| P15 | -/21/20/29; VOC B1.1.7a | B.1.1.7 | del69-70, del144, N501Y, A570D, D614G, P681H, A694AS, T716I, S982A, D1118H |
Abbreviations: CT, cycle threshold resulting from RT-PCR performed at St. Josef-Hospital Bochum; VOC, variants of concern.
NOTE: Data are presented as individual data. Lineage of deep sequencing is presented according to the pangolin tool [13]. Bold = characteristic mutations associated with VOC according to Robert Koch Institute [17].
VOC analyses started in the midst of the study period in February 2020 and was performed via melting curve analysis.
No sufficient material available.
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