| Synaptic compartment . | General class . | Drosophila protein . | Vertebrate ortholog (murine) . | Labeled reagent . | Expression approach . | BDSC Stock ID . | References . |
|---|---|---|---|---|---|---|---|
| Presynaptic | AZ | Bruchpilot | Erc2 | Brp-FL::GFP | Inducible (STaR): FLP/FRT | BDSC: 55759 | Chen et al. (2014) |
| Brp-FL::smV5 | Inducible (STaR): FLP/FRT | n/a | Peng et al. (2018) | ||||
| Brp-FL::V5 | Inducible (STaR): FLP/FRT | BDSC: 55760 | Chen et al. (2014) | ||||
| Brp-FL::GFP | Binary expression: Gal4/UAS | BDSC: 36291 | Wagh et al. (2006) | ||||
| Brp-Short::GFP | Binary expression: Gal4/UAS | n/a | Fouquet et al. (2009) | ||||
| Brp-Short::mStrawberry | Binary expression: Gal4/UAS | n/a | Fouquet et al. (2009) | ||||
| Brp-Short::mStrawberry | Binary expression: QF/QUAS | BDSC: 80571 | Mosca and Luo (2014) | ||||
| Brp-Short::CFP | Binary expression: Gal4/UAS | n/a | Schmid et al. (2008) | ||||
| Brp-Short::Cherry | Binary expression: Gal4/UAS | n/a | Fouquet et al. (2009) and Kremer et al. (2010) | ||||
| Brp-Short::Cherry | Binary expression: LexA/LexAop | n/a | Berger-Müller et al. (2013) | ||||
| Cacophony | Cacna1a | Cac-1::EGFP | Binary expression: Gal4/UAS | BDSC: 8582 | Kawasaki et al. (2004) | ||
| Liprin-α | Ppfia2 | GFP::Liprin-α | Binary expression: Gal4/UAS | n/a | Fouquet et al. (2009) | ||
| RhoGAP100F (Syd-1) | Syde1 | GFP::Syd-1 | Binary expression: Gal4/UAS | n/a | Owald et al. (2010) | ||
| RBP | Tspoap1 | GFP::DRBP | Binary expression: Gal4/UAS | n/a | Liu et al. (2011) | ||
| Unc-13 | Unc13a | Unc-13A::GFP | Binary expression: Gal4/UAS | n/a | Böhme et al. (2016) | ||
| Unc-13B::GFP | Binary expression: Gal4/UAS | n/a | Böhme et al. (2016) | ||||
| Synaptic Vesicle | neural Synaptobrevin | Vamp2 | nSyb::EGFP | Binary expression: Gal4/UAS | BDSC 9263 | Estes et al. (2000) | |
| Rab3 | Rab3c | GFP::Rab3 | Inducible: B2R/B2RT recombination (gene edit) | BDSC: 81502 | Williams et al. (2019) | ||
| mCherry::Rab3 | Inducible: B2R/B2RT recombination (gene edit) | BDSC: 81503 | |||||
| 3xFLAG::Rab3 | Inducible: FLP/FRT recombination (gene edit) | BDSC: 81501 | |||||
| 2xHA::Rab3 | Inducible: FLP/FRT recombination (gene edit) | BDSC: 81500 | |||||
| Synaptotagmin 1 | Syt I | Syt-1::EGFP | Binary expression: Gal4/UAS | BDSC 6926 | Zhang et al. (2002) | ||
| Vesicular Glutamate Transporter | vGlut | smFLAG::vGlut | Inducible: RSR/RSRT recombination (gene edit) | n/a | Certel, Ruchti, et al. (2022) | ||
| Vesicular GABA Transporter | Slc32a1 | 9xV5::VGAT | Inducible: RSR/RSRT recombination (gene edit) | n/a | Certel, McCabe, et al. (2022) | ||
| Postsynaptic | Dendritic compartment | No homolog | Icam5 | Icam5::mCherry (DenMark) | Binary expression: Gal4/UAS | BDSC: 33062 | Nicolaï et al. (2010) |
| NT receptor | GluClα | Glra1 | GluClα::GFP | Binary expression: Gal4/UAS | BDSC: 92149 | Fendl et al. (2020) | |
| GluClα::GFP | Inducible (FlpTag): FLP/FRT | BDSC: 92145 | |||||
| GABA-B receptor 1 | Gabbr1 | GABABR1::GFP | Inducible (FlpTag): FLP/FRT | BDSC: 92148 | |||
| Acetylcholine Receptor α7 (Dα7) | Chrna7 | Dα7::GFP | Binary expression: Gal4/UAS | BDSC: 39692 | Leiss et al. (2009) | ||
| Ort (Ora transientless) | Unclear | OLLAS::Ort | Inducible (STaR): FLP/FRT | BDSC: 55761 | Chen et al. (2014) | ||
| Rdl | Gabrp | Rdl::HA | Binary expression: Gal4/UAS | BDSC: 29035 | Sánchez-Soriano et al. (2005) | ||
| Rdl::GFP | Binary expression: Gal4/UAS | BDSC: 92150 | Fendl et al. (2020) | ||||
| Glutamatergic postsynapse | Drep-2 | Cidea | Drep-2::EGFP | Binary expression: Gal4/UAS | n/a | Andlauer et al. (2014) | |
| Drep-2::mStrawberry | Binary expression: Gal4/UAS | n/a | |||||
| Synaptic compartment . | General class . | Drosophila protein . | Vertebrate ortholog (murine) . | Labeled reagent . | Expression approach . | BDSC Stock ID . | References . |
|---|---|---|---|---|---|---|---|
| Presynaptic | AZ | Bruchpilot | Erc2 | Brp-FL::GFP | Inducible (STaR): FLP/FRT | BDSC: 55759 | Chen et al. (2014) |
| Brp-FL::smV5 | Inducible (STaR): FLP/FRT | n/a | Peng et al. (2018) | ||||
| Brp-FL::V5 | Inducible (STaR): FLP/FRT | BDSC: 55760 | Chen et al. (2014) | ||||
| Brp-FL::GFP | Binary expression: Gal4/UAS | BDSC: 36291 | Wagh et al. (2006) | ||||
| Brp-Short::GFP | Binary expression: Gal4/UAS | n/a | Fouquet et al. (2009) | ||||
| Brp-Short::mStrawberry | Binary expression: Gal4/UAS | n/a | Fouquet et al. (2009) | ||||
| Brp-Short::mStrawberry | Binary expression: QF/QUAS | BDSC: 80571 | Mosca and Luo (2014) | ||||
| Brp-Short::CFP | Binary expression: Gal4/UAS | n/a | Schmid et al. (2008) | ||||
| Brp-Short::Cherry | Binary expression: Gal4/UAS | n/a | Fouquet et al. (2009) and Kremer et al. (2010) | ||||
| Brp-Short::Cherry | Binary expression: LexA/LexAop | n/a | Berger-Müller et al. (2013) | ||||
| Cacophony | Cacna1a | Cac-1::EGFP | Binary expression: Gal4/UAS | BDSC: 8582 | Kawasaki et al. (2004) | ||
| Liprin-α | Ppfia2 | GFP::Liprin-α | Binary expression: Gal4/UAS | n/a | Fouquet et al. (2009) | ||
| RhoGAP100F (Syd-1) | Syde1 | GFP::Syd-1 | Binary expression: Gal4/UAS | n/a | Owald et al. (2010) | ||
| RBP | Tspoap1 | GFP::DRBP | Binary expression: Gal4/UAS | n/a | Liu et al. (2011) | ||
| Unc-13 | Unc13a | Unc-13A::GFP | Binary expression: Gal4/UAS | n/a | Böhme et al. (2016) | ||
| Unc-13B::GFP | Binary expression: Gal4/UAS | n/a | Böhme et al. (2016) | ||||
| Synaptic Vesicle | neural Synaptobrevin | Vamp2 | nSyb::EGFP | Binary expression: Gal4/UAS | BDSC 9263 | Estes et al. (2000) | |
| Rab3 | Rab3c | GFP::Rab3 | Inducible: B2R/B2RT recombination (gene edit) | BDSC: 81502 | Williams et al. (2019) | ||
| mCherry::Rab3 | Inducible: B2R/B2RT recombination (gene edit) | BDSC: 81503 | |||||
| 3xFLAG::Rab3 | Inducible: FLP/FRT recombination (gene edit) | BDSC: 81501 | |||||
| 2xHA::Rab3 | Inducible: FLP/FRT recombination (gene edit) | BDSC: 81500 | |||||
| Synaptotagmin 1 | Syt I | Syt-1::EGFP | Binary expression: Gal4/UAS | BDSC 6926 | Zhang et al. (2002) | ||
| Vesicular Glutamate Transporter | vGlut | smFLAG::vGlut | Inducible: RSR/RSRT recombination (gene edit) | n/a | Certel, Ruchti, et al. (2022) | ||
| Vesicular GABA Transporter | Slc32a1 | 9xV5::VGAT | Inducible: RSR/RSRT recombination (gene edit) | n/a | Certel, McCabe, et al. (2022) | ||
| Postsynaptic | Dendritic compartment | No homolog | Icam5 | Icam5::mCherry (DenMark) | Binary expression: Gal4/UAS | BDSC: 33062 | Nicolaï et al. (2010) |
| NT receptor | GluClα | Glra1 | GluClα::GFP | Binary expression: Gal4/UAS | BDSC: 92149 | Fendl et al. (2020) | |
| GluClα::GFP | Inducible (FlpTag): FLP/FRT | BDSC: 92145 | |||||
| GABA-B receptor 1 | Gabbr1 | GABABR1::GFP | Inducible (FlpTag): FLP/FRT | BDSC: 92148 | |||
| Acetylcholine Receptor α7 (Dα7) | Chrna7 | Dα7::GFP | Binary expression: Gal4/UAS | BDSC: 39692 | Leiss et al. (2009) | ||
| Ort (Ora transientless) | Unclear | OLLAS::Ort | Inducible (STaR): FLP/FRT | BDSC: 55761 | Chen et al. (2014) | ||
| Rdl | Gabrp | Rdl::HA | Binary expression: Gal4/UAS | BDSC: 29035 | Sánchez-Soriano et al. (2005) | ||
| Rdl::GFP | Binary expression: Gal4/UAS | BDSC: 92150 | Fendl et al. (2020) | ||||
| Glutamatergic postsynapse | Drep-2 | Cidea | Drep-2::EGFP | Binary expression: Gal4/UAS | n/a | Andlauer et al. (2014) | |
| Drep-2::mStrawberry | Binary expression: Gal4/UAS | n/a | |||||
A selection of these tools is highlighted in Parts I and II of this review. This table is intended as a general aid, but should not be considered an exhaustive list of available reagents.
| Synaptic compartment . | General class . | Drosophila protein . | Vertebrate ortholog (murine) . | Labeled reagent . | Expression approach . | BDSC Stock ID . | References . |
|---|---|---|---|---|---|---|---|
| Presynaptic | AZ | Bruchpilot | Erc2 | Brp-FL::GFP | Inducible (STaR): FLP/FRT | BDSC: 55759 | Chen et al. (2014) |
| Brp-FL::smV5 | Inducible (STaR): FLP/FRT | n/a | Peng et al. (2018) | ||||
| Brp-FL::V5 | Inducible (STaR): FLP/FRT | BDSC: 55760 | Chen et al. (2014) | ||||
| Brp-FL::GFP | Binary expression: Gal4/UAS | BDSC: 36291 | Wagh et al. (2006) | ||||
| Brp-Short::GFP | Binary expression: Gal4/UAS | n/a | Fouquet et al. (2009) | ||||
| Brp-Short::mStrawberry | Binary expression: Gal4/UAS | n/a | Fouquet et al. (2009) | ||||
| Brp-Short::mStrawberry | Binary expression: QF/QUAS | BDSC: 80571 | Mosca and Luo (2014) | ||||
| Brp-Short::CFP | Binary expression: Gal4/UAS | n/a | Schmid et al. (2008) | ||||
| Brp-Short::Cherry | Binary expression: Gal4/UAS | n/a | Fouquet et al. (2009) and Kremer et al. (2010) | ||||
| Brp-Short::Cherry | Binary expression: LexA/LexAop | n/a | Berger-Müller et al. (2013) | ||||
| Cacophony | Cacna1a | Cac-1::EGFP | Binary expression: Gal4/UAS | BDSC: 8582 | Kawasaki et al. (2004) | ||
| Liprin-α | Ppfia2 | GFP::Liprin-α | Binary expression: Gal4/UAS | n/a | Fouquet et al. (2009) | ||
| RhoGAP100F (Syd-1) | Syde1 | GFP::Syd-1 | Binary expression: Gal4/UAS | n/a | Owald et al. (2010) | ||
| RBP | Tspoap1 | GFP::DRBP | Binary expression: Gal4/UAS | n/a | Liu et al. (2011) | ||
| Unc-13 | Unc13a | Unc-13A::GFP | Binary expression: Gal4/UAS | n/a | Böhme et al. (2016) | ||
| Unc-13B::GFP | Binary expression: Gal4/UAS | n/a | Böhme et al. (2016) | ||||
| Synaptic Vesicle | neural Synaptobrevin | Vamp2 | nSyb::EGFP | Binary expression: Gal4/UAS | BDSC 9263 | Estes et al. (2000) | |
| Rab3 | Rab3c | GFP::Rab3 | Inducible: B2R/B2RT recombination (gene edit) | BDSC: 81502 | Williams et al. (2019) | ||
| mCherry::Rab3 | Inducible: B2R/B2RT recombination (gene edit) | BDSC: 81503 | |||||
| 3xFLAG::Rab3 | Inducible: FLP/FRT recombination (gene edit) | BDSC: 81501 | |||||
| 2xHA::Rab3 | Inducible: FLP/FRT recombination (gene edit) | BDSC: 81500 | |||||
| Synaptotagmin 1 | Syt I | Syt-1::EGFP | Binary expression: Gal4/UAS | BDSC 6926 | Zhang et al. (2002) | ||
| Vesicular Glutamate Transporter | vGlut | smFLAG::vGlut | Inducible: RSR/RSRT recombination (gene edit) | n/a | Certel, Ruchti, et al. (2022) | ||
| Vesicular GABA Transporter | Slc32a1 | 9xV5::VGAT | Inducible: RSR/RSRT recombination (gene edit) | n/a | Certel, McCabe, et al. (2022) | ||
| Postsynaptic | Dendritic compartment | No homolog | Icam5 | Icam5::mCherry (DenMark) | Binary expression: Gal4/UAS | BDSC: 33062 | Nicolaï et al. (2010) |
| NT receptor | GluClα | Glra1 | GluClα::GFP | Binary expression: Gal4/UAS | BDSC: 92149 | Fendl et al. (2020) | |
| GluClα::GFP | Inducible (FlpTag): FLP/FRT | BDSC: 92145 | |||||
| GABA-B receptor 1 | Gabbr1 | GABABR1::GFP | Inducible (FlpTag): FLP/FRT | BDSC: 92148 | |||
| Acetylcholine Receptor α7 (Dα7) | Chrna7 | Dα7::GFP | Binary expression: Gal4/UAS | BDSC: 39692 | Leiss et al. (2009) | ||
| Ort (Ora transientless) | Unclear | OLLAS::Ort | Inducible (STaR): FLP/FRT | BDSC: 55761 | Chen et al. (2014) | ||
| Rdl | Gabrp | Rdl::HA | Binary expression: Gal4/UAS | BDSC: 29035 | Sánchez-Soriano et al. (2005) | ||
| Rdl::GFP | Binary expression: Gal4/UAS | BDSC: 92150 | Fendl et al. (2020) | ||||
| Glutamatergic postsynapse | Drep-2 | Cidea | Drep-2::EGFP | Binary expression: Gal4/UAS | n/a | Andlauer et al. (2014) | |
| Drep-2::mStrawberry | Binary expression: Gal4/UAS | n/a | |||||
| Synaptic compartment . | General class . | Drosophila protein . | Vertebrate ortholog (murine) . | Labeled reagent . | Expression approach . | BDSC Stock ID . | References . |
|---|---|---|---|---|---|---|---|
| Presynaptic | AZ | Bruchpilot | Erc2 | Brp-FL::GFP | Inducible (STaR): FLP/FRT | BDSC: 55759 | Chen et al. (2014) |
| Brp-FL::smV5 | Inducible (STaR): FLP/FRT | n/a | Peng et al. (2018) | ||||
| Brp-FL::V5 | Inducible (STaR): FLP/FRT | BDSC: 55760 | Chen et al. (2014) | ||||
| Brp-FL::GFP | Binary expression: Gal4/UAS | BDSC: 36291 | Wagh et al. (2006) | ||||
| Brp-Short::GFP | Binary expression: Gal4/UAS | n/a | Fouquet et al. (2009) | ||||
| Brp-Short::mStrawberry | Binary expression: Gal4/UAS | n/a | Fouquet et al. (2009) | ||||
| Brp-Short::mStrawberry | Binary expression: QF/QUAS | BDSC: 80571 | Mosca and Luo (2014) | ||||
| Brp-Short::CFP | Binary expression: Gal4/UAS | n/a | Schmid et al. (2008) | ||||
| Brp-Short::Cherry | Binary expression: Gal4/UAS | n/a | Fouquet et al. (2009) and Kremer et al. (2010) | ||||
| Brp-Short::Cherry | Binary expression: LexA/LexAop | n/a | Berger-Müller et al. (2013) | ||||
| Cacophony | Cacna1a | Cac-1::EGFP | Binary expression: Gal4/UAS | BDSC: 8582 | Kawasaki et al. (2004) | ||
| Liprin-α | Ppfia2 | GFP::Liprin-α | Binary expression: Gal4/UAS | n/a | Fouquet et al. (2009) | ||
| RhoGAP100F (Syd-1) | Syde1 | GFP::Syd-1 | Binary expression: Gal4/UAS | n/a | Owald et al. (2010) | ||
| RBP | Tspoap1 | GFP::DRBP | Binary expression: Gal4/UAS | n/a | Liu et al. (2011) | ||
| Unc-13 | Unc13a | Unc-13A::GFP | Binary expression: Gal4/UAS | n/a | Böhme et al. (2016) | ||
| Unc-13B::GFP | Binary expression: Gal4/UAS | n/a | Böhme et al. (2016) | ||||
| Synaptic Vesicle | neural Synaptobrevin | Vamp2 | nSyb::EGFP | Binary expression: Gal4/UAS | BDSC 9263 | Estes et al. (2000) | |
| Rab3 | Rab3c | GFP::Rab3 | Inducible: B2R/B2RT recombination (gene edit) | BDSC: 81502 | Williams et al. (2019) | ||
| mCherry::Rab3 | Inducible: B2R/B2RT recombination (gene edit) | BDSC: 81503 | |||||
| 3xFLAG::Rab3 | Inducible: FLP/FRT recombination (gene edit) | BDSC: 81501 | |||||
| 2xHA::Rab3 | Inducible: FLP/FRT recombination (gene edit) | BDSC: 81500 | |||||
| Synaptotagmin 1 | Syt I | Syt-1::EGFP | Binary expression: Gal4/UAS | BDSC 6926 | Zhang et al. (2002) | ||
| Vesicular Glutamate Transporter | vGlut | smFLAG::vGlut | Inducible: RSR/RSRT recombination (gene edit) | n/a | Certel, Ruchti, et al. (2022) | ||
| Vesicular GABA Transporter | Slc32a1 | 9xV5::VGAT | Inducible: RSR/RSRT recombination (gene edit) | n/a | Certel, McCabe, et al. (2022) | ||
| Postsynaptic | Dendritic compartment | No homolog | Icam5 | Icam5::mCherry (DenMark) | Binary expression: Gal4/UAS | BDSC: 33062 | Nicolaï et al. (2010) |
| NT receptor | GluClα | Glra1 | GluClα::GFP | Binary expression: Gal4/UAS | BDSC: 92149 | Fendl et al. (2020) | |
| GluClα::GFP | Inducible (FlpTag): FLP/FRT | BDSC: 92145 | |||||
| GABA-B receptor 1 | Gabbr1 | GABABR1::GFP | Inducible (FlpTag): FLP/FRT | BDSC: 92148 | |||
| Acetylcholine Receptor α7 (Dα7) | Chrna7 | Dα7::GFP | Binary expression: Gal4/UAS | BDSC: 39692 | Leiss et al. (2009) | ||
| Ort (Ora transientless) | Unclear | OLLAS::Ort | Inducible (STaR): FLP/FRT | BDSC: 55761 | Chen et al. (2014) | ||
| Rdl | Gabrp | Rdl::HA | Binary expression: Gal4/UAS | BDSC: 29035 | Sánchez-Soriano et al. (2005) | ||
| Rdl::GFP | Binary expression: Gal4/UAS | BDSC: 92150 | Fendl et al. (2020) | ||||
| Glutamatergic postsynapse | Drep-2 | Cidea | Drep-2::EGFP | Binary expression: Gal4/UAS | n/a | Andlauer et al. (2014) | |
| Drep-2::mStrawberry | Binary expression: Gal4/UAS | n/a | |||||
A selection of these tools is highlighted in Parts I and II of this review. This table is intended as a general aid, but should not be considered an exhaustive list of available reagents.
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