Proportion of ricinoleate at position sn-2 of [14C]glycerol labeled 1-OH-DAG and DAG derived 1-OH-PC formed from [14C]G3P and a mixture of 18:3-CoA and ricinoleoyl-CoA in yeast membranes expressing castor PDCT
| [14C]Glycerol labeled lipid . | Ricinoleate (%) . |
|---|---|
| Sn-2-1-OH-DAG | 38.4 ± 5.7 |
| Sn-2-1-OH-PC | 78.2 ± 2.9 |
| [14C]Glycerol labeled lipid . | Ricinoleate (%) . |
|---|---|
| Sn-2-1-OH-DAG | 38.4 ± 5.7 |
| Sn-2-1-OH-PC | 78.2 ± 2.9 |
Assay conditions were identical to those presented in Figure 2C. 1-OH-DAG, DAG with one ricinoleoyl group; 1-OH-PC, PC with one ricinoleoyl group. Average value shown ± sd, n = 3 replicates.
Proportion of ricinoleate at position sn-2 of [14C]glycerol labeled 1-OH-DAG and DAG derived 1-OH-PC formed from [14C]G3P and a mixture of 18:3-CoA and ricinoleoyl-CoA in yeast membranes expressing castor PDCT
| [14C]Glycerol labeled lipid . | Ricinoleate (%) . |
|---|---|
| Sn-2-1-OH-DAG | 38.4 ± 5.7 |
| Sn-2-1-OH-PC | 78.2 ± 2.9 |
| [14C]Glycerol labeled lipid . | Ricinoleate (%) . |
|---|---|
| Sn-2-1-OH-DAG | 38.4 ± 5.7 |
| Sn-2-1-OH-PC | 78.2 ± 2.9 |
Assay conditions were identical to those presented in Figure 2C. 1-OH-DAG, DAG with one ricinoleoyl group; 1-OH-PC, PC with one ricinoleoyl group. Average value shown ± sd, n = 3 replicates.
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