Table 2
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Mass spectrometry analysis of co-immunoprecipitated proteins using each NP1 variants as bait

Co-immunoprecipitated proteinsImmunoprecipitated proteins
(peptide number of sample/control)
NP1 WTNP1 E327GNP1 G389R
ERNP1455363451
HSPA5 (Bip chaperone)10854102
PDIA6 (ER chaperone)773068
CANX (Ca binding protein)281721
HSP90B1 (ER chaperone)231523
PDIA3 (disulphite isomerase)1010
Nucleus/cytoplasmEEF1A1(elongation factor)343234
NES (nestin)20
BAG2 (cytosolic chaperone)1414
BAG6 (cytosolic chaperone)14
NIF3L1 (mRNA nuclear export)12
TCP1 (cytosolic chaperone)1111
CytoskeletonVIM (vimentin)612
PLEC (plectin)370
ACTB (actin)3918736
MYH1096
MYH1876
SPTA1 (spectrin)65
SPTB (spectrin)50
KRT7 (sarcolectin)41
KRT1834
GSN (gelsolin)19
DBN116
MYO1B14
TMOD310
MitochondriaHSPD11720
ATP5F1A171111
Co-immunoprecipitated proteinsImmunoprecipitated proteins
(peptide number of sample/control)
NP1 WTNP1 E327GNP1 G389R
ERNP1455363451
HSPA5 (Bip chaperone)10854102
PDIA6 (ER chaperone)773068
CANX (Ca binding protein)281721
HSP90B1 (ER chaperone)231523
PDIA3 (disulphite isomerase)1010
Nucleus/cytoplasmEEF1A1(elongation factor)343234
NES (nestin)20
BAG2 (cytosolic chaperone)1414
BAG6 (cytosolic chaperone)14
NIF3L1 (mRNA nuclear export)12
TCP1 (cytosolic chaperone)1111
CytoskeletonVIM (vimentin)612
PLEC (plectin)370
ACTB (actin)3918736
MYH1096
MYH1876
SPTA1 (spectrin)65
SPTB (spectrin)50
KRT7 (sarcolectin)41
KRT1834
GSN (gelsolin)19
DBN116
MYO1B14
TMOD310
MitochondriaHSPD11720
ATP5F1A171111

Listed are proteins identified with at least 10 peptides. WT = wild-type.

Table 2
Open in new tab

Mass spectrometry analysis of co-immunoprecipitated proteins using each NP1 variants as bait

Co-immunoprecipitated proteinsImmunoprecipitated proteins
(peptide number of sample/control)
NP1 WTNP1 E327GNP1 G389R
ERNP1455363451
HSPA5 (Bip chaperone)10854102
PDIA6 (ER chaperone)773068
CANX (Ca binding protein)281721
HSP90B1 (ER chaperone)231523
PDIA3 (disulphite isomerase)1010
Nucleus/cytoplasmEEF1A1(elongation factor)343234
NES (nestin)20
BAG2 (cytosolic chaperone)1414
BAG6 (cytosolic chaperone)14
NIF3L1 (mRNA nuclear export)12
TCP1 (cytosolic chaperone)1111
CytoskeletonVIM (vimentin)612
PLEC (plectin)370
ACTB (actin)3918736
MYH1096
MYH1876
SPTA1 (spectrin)65
SPTB (spectrin)50
KRT7 (sarcolectin)41
KRT1834
GSN (gelsolin)19
DBN116
MYO1B14
TMOD310
MitochondriaHSPD11720
ATP5F1A171111
Co-immunoprecipitated proteinsImmunoprecipitated proteins
(peptide number of sample/control)
NP1 WTNP1 E327GNP1 G389R
ERNP1455363451
HSPA5 (Bip chaperone)10854102
PDIA6 (ER chaperone)773068
CANX (Ca binding protein)281721
HSP90B1 (ER chaperone)231523
PDIA3 (disulphite isomerase)1010
Nucleus/cytoplasmEEF1A1(elongation factor)343234
NES (nestin)20
BAG2 (cytosolic chaperone)1414
BAG6 (cytosolic chaperone)14
NIF3L1 (mRNA nuclear export)12
TCP1 (cytosolic chaperone)1111
CytoskeletonVIM (vimentin)612
PLEC (plectin)370
ACTB (actin)3918736
MYH1096
MYH1876
SPTA1 (spectrin)65
SPTB (spectrin)50
KRT7 (sarcolectin)41
KRT1834
GSN (gelsolin)19
DBN116
MYO1B14
TMOD310
MitochondriaHSPD11720
ATP5F1A171111

Listed are proteins identified with at least 10 peptides. WT = wild-type.

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