Table 6.

Chemical, amino acid, and fatty acid composition of Tenebrio molitor larvae at 90 d post-hatching with the different experimental diets (n = 36 trays; degrees of freedom: 2 for diets, 2 for laying period and 1 for batch)

DietsSEMF-valueP-value
WBFCWBG
Chemical composition (g/100 g)
 Dry matter (DM)35.4234.4435.420.382.210.1243
 Ashes3.583.623.640.090.100.9029
 Ether extract32.3533.0632.560.560.410.6698
 Crude protein54.7153.8054.800.421.680.2014
Amino acid composition (g/100 g DM)
 Alanine4.012b4.098b3.916a0.0255.790.0066
 Arginine2.866b2.534a2.447a0.0764.520.0177
 Aspartic acid4.9684.7205.5700.3601.240.3014
 Cysteine0.5140.5340.5030.0290.300.7450
 Glutamic acid6.8156.8856.5830.1730.750.4785
 Glycine2.5932.6402.5000.0670.990.3830
 Histidine1.636b1.482a1.606ab0.0363.210.0520
 Isoleucine2.2972.2942.2560.0420.290.7506
 Leucine3.9133.8233.9040.0810.360.7002
 Lysine3.0322.9622.9690.0390.890.4213
 Methionine0.795a0.848b0.772ab0.0222.320.1128
 Phenylalanine1.930b1.822a1.907ab0.0253.260.0501
 Proline3.1773.1683.0800.0431.280.2893
 Serine2.6012.5292.8020.1730.620.5461
 Threonine2.120b1.812a1.798a0.0733.670.0354
 Tyrosine3.814b3.735a3.892c0.0168.040.0013
 Valine3.5513.5403.4940.0390.560.5762
Fatty acid composition (% total fatty acids)
 Lauric acid (C12:0)0.1310.1470.1310.0081.140.3319
 Myristic acid (C14:0)1.6781.7191.6600.0650.210.8152
 Pentadecanoic acid (C15:0)0.167b0.152a0.168b0.0035.460.0085
 Palmitic acid (C16:0)16.8216.9816.970.110.690.5103
 Palmitoleic acid (C16:1)1.124a1.220b1.112a0.0137.230.0023
 Heptadecanoic acid (C17:0)0.2140.2100.2280.0110.650.5257
 Cis-10-Heptadecenoic acid (C17:1)0.2210.1610.1820.0162.300.1140
 Stearic acid (C18:0)3.2703.2293.2980.0490.460.6351
 Elaidic acid (C18:1n9t)0.0450.0480.0440.0021.010.3731
 Oleic acid (C18:1n9c)32.59ab34.44b32.09a0.5812.990.0628
 Linoleic acid (C18:2n6c)40.28b38.35a40.62b0.493.730.0337
 Arachidic acid (C20:0)0.1100.0980.1070.0051.520.2323
 G-Linolenic acid (C18:3n6)0.0490.0510.0510.0030.150.8573
 Cis-11-Eicosenoic acid (C20:1)0.251b0.223a0.243b0.0037.570.0018
 Linolenic acid (C18:3n3)2.7712.7002.8250.0700.720.4955
 Cis-11,14-Eicosadienoic acid (C20:2)0.0880.0740.0820.0042.180.1280
 Cis-11,14,17-Eicosatrienoic acid (C20:3n3)0.0950.0970.1000.0030.550.5844
 Arachidonic acid (C20:4n6)0.0960.0860.0910.0050.910.4103
 SFA22.3822.5322.560.100.800.4592
 MUFA34.23ab36.10b33.67a0.593.060.0594
 PUFA43.38b41.37a43.76b0.553.370.0457
 n-32.8662.8012.9210.0700.680.5147
 n-640.43b38.49a40.76b0.493.700.0345
 n-6/n-314.1113.7513.960.190.780.4662
 PUFA/SFA1.938b1.835a1.939b0.0243.630.0367
 SFA/UFA0.2880.2910.2910.0020.800.4589
 AI0.3050.3100.3070.0030.780.4665
 TI0.4730.4790.4760.0030.970.3889
DietsSEMF-valueP-value
WBFCWBG
Chemical composition (g/100 g)
 Dry matter (DM)35.4234.4435.420.382.210.1243
 Ashes3.583.623.640.090.100.9029
 Ether extract32.3533.0632.560.560.410.6698
 Crude protein54.7153.8054.800.421.680.2014
Amino acid composition (g/100 g DM)
 Alanine4.012b4.098b3.916a0.0255.790.0066
 Arginine2.866b2.534a2.447a0.0764.520.0177
 Aspartic acid4.9684.7205.5700.3601.240.3014
 Cysteine0.5140.5340.5030.0290.300.7450
 Glutamic acid6.8156.8856.5830.1730.750.4785
 Glycine2.5932.6402.5000.0670.990.3830
 Histidine1.636b1.482a1.606ab0.0363.210.0520
 Isoleucine2.2972.2942.2560.0420.290.7506
 Leucine3.9133.8233.9040.0810.360.7002
 Lysine3.0322.9622.9690.0390.890.4213
 Methionine0.795a0.848b0.772ab0.0222.320.1128
 Phenylalanine1.930b1.822a1.907ab0.0253.260.0501
 Proline3.1773.1683.0800.0431.280.2893
 Serine2.6012.5292.8020.1730.620.5461
 Threonine2.120b1.812a1.798a0.0733.670.0354
 Tyrosine3.814b3.735a3.892c0.0168.040.0013
 Valine3.5513.5403.4940.0390.560.5762
Fatty acid composition (% total fatty acids)
 Lauric acid (C12:0)0.1310.1470.1310.0081.140.3319
 Myristic acid (C14:0)1.6781.7191.6600.0650.210.8152
 Pentadecanoic acid (C15:0)0.167b0.152a0.168b0.0035.460.0085
 Palmitic acid (C16:0)16.8216.9816.970.110.690.5103
 Palmitoleic acid (C16:1)1.124a1.220b1.112a0.0137.230.0023
 Heptadecanoic acid (C17:0)0.2140.2100.2280.0110.650.5257
 Cis-10-Heptadecenoic acid (C17:1)0.2210.1610.1820.0162.300.1140
 Stearic acid (C18:0)3.2703.2293.2980.0490.460.6351
 Elaidic acid (C18:1n9t)0.0450.0480.0440.0021.010.3731
 Oleic acid (C18:1n9c)32.59ab34.44b32.09a0.5812.990.0628
 Linoleic acid (C18:2n6c)40.28b38.35a40.62b0.493.730.0337
 Arachidic acid (C20:0)0.1100.0980.1070.0051.520.2323
 G-Linolenic acid (C18:3n6)0.0490.0510.0510.0030.150.8573
 Cis-11-Eicosenoic acid (C20:1)0.251b0.223a0.243b0.0037.570.0018
 Linolenic acid (C18:3n3)2.7712.7002.8250.0700.720.4955
 Cis-11,14-Eicosadienoic acid (C20:2)0.0880.0740.0820.0042.180.1280
 Cis-11,14,17-Eicosatrienoic acid (C20:3n3)0.0950.0970.1000.0030.550.5844
 Arachidonic acid (C20:4n6)0.0960.0860.0910.0050.910.4103
 SFA22.3822.5322.560.100.800.4592
 MUFA34.23ab36.10b33.67a0.593.060.0594
 PUFA43.38b41.37a43.76b0.553.370.0457
 n-32.8662.8012.9210.0700.680.5147
 n-640.43b38.49a40.76b0.493.700.0345
 n-6/n-314.1113.7513.960.190.780.4662
 PUFA/SFA1.938b1.835a1.939b0.0243.630.0367
 SFA/UFA0.2880.2910.2910.0020.800.4589
 AI0.3050.3100.3070.0030.780.4665
 TI0.4730.4790.4760.0030.970.3889

WB: diet including wheat bran and water; FC: diet including wheat bran and fresh carrots; WBG: diet including wheat bran, wet brewer’s grains and water (see Table 3). SEM: standard error of the means.

a,b,cMeans in the same row with no common superscripts differ significantly (P < 0.05). SFA,saturated fatty acids [C14:0 + C15:0 + C16:0 + C17:0 + C18:0]; MUFA, monounsaturated fatty acids; PUFA, polyunsaturated fatty acids; n-3: Omega-3 fatty acids; n-6: Omega-6 fatty acids; PUFA/SFA: ratio PUFA/SFA; SFA/UFA: ratio SFA/(MUFA + PUFA); AI, atherogenic index; TI, thrombogenic index.

Table 6.

Chemical, amino acid, and fatty acid composition of Tenebrio molitor larvae at 90 d post-hatching with the different experimental diets (n = 36 trays; degrees of freedom: 2 for diets, 2 for laying period and 1 for batch)

DietsSEMF-valueP-value
WBFCWBG
Chemical composition (g/100 g)
 Dry matter (DM)35.4234.4435.420.382.210.1243
 Ashes3.583.623.640.090.100.9029
 Ether extract32.3533.0632.560.560.410.6698
 Crude protein54.7153.8054.800.421.680.2014
Amino acid composition (g/100 g DM)
 Alanine4.012b4.098b3.916a0.0255.790.0066
 Arginine2.866b2.534a2.447a0.0764.520.0177
 Aspartic acid4.9684.7205.5700.3601.240.3014
 Cysteine0.5140.5340.5030.0290.300.7450
 Glutamic acid6.8156.8856.5830.1730.750.4785
 Glycine2.5932.6402.5000.0670.990.3830
 Histidine1.636b1.482a1.606ab0.0363.210.0520
 Isoleucine2.2972.2942.2560.0420.290.7506
 Leucine3.9133.8233.9040.0810.360.7002
 Lysine3.0322.9622.9690.0390.890.4213
 Methionine0.795a0.848b0.772ab0.0222.320.1128
 Phenylalanine1.930b1.822a1.907ab0.0253.260.0501
 Proline3.1773.1683.0800.0431.280.2893
 Serine2.6012.5292.8020.1730.620.5461
 Threonine2.120b1.812a1.798a0.0733.670.0354
 Tyrosine3.814b3.735a3.892c0.0168.040.0013
 Valine3.5513.5403.4940.0390.560.5762
Fatty acid composition (% total fatty acids)
 Lauric acid (C12:0)0.1310.1470.1310.0081.140.3319
 Myristic acid (C14:0)1.6781.7191.6600.0650.210.8152
 Pentadecanoic acid (C15:0)0.167b0.152a0.168b0.0035.460.0085
 Palmitic acid (C16:0)16.8216.9816.970.110.690.5103
 Palmitoleic acid (C16:1)1.124a1.220b1.112a0.0137.230.0023
 Heptadecanoic acid (C17:0)0.2140.2100.2280.0110.650.5257
 Cis-10-Heptadecenoic acid (C17:1)0.2210.1610.1820.0162.300.1140
 Stearic acid (C18:0)3.2703.2293.2980.0490.460.6351
 Elaidic acid (C18:1n9t)0.0450.0480.0440.0021.010.3731
 Oleic acid (C18:1n9c)32.59ab34.44b32.09a0.5812.990.0628
 Linoleic acid (C18:2n6c)40.28b38.35a40.62b0.493.730.0337
 Arachidic acid (C20:0)0.1100.0980.1070.0051.520.2323
 G-Linolenic acid (C18:3n6)0.0490.0510.0510.0030.150.8573
 Cis-11-Eicosenoic acid (C20:1)0.251b0.223a0.243b0.0037.570.0018
 Linolenic acid (C18:3n3)2.7712.7002.8250.0700.720.4955
 Cis-11,14-Eicosadienoic acid (C20:2)0.0880.0740.0820.0042.180.1280
 Cis-11,14,17-Eicosatrienoic acid (C20:3n3)0.0950.0970.1000.0030.550.5844
 Arachidonic acid (C20:4n6)0.0960.0860.0910.0050.910.4103
 SFA22.3822.5322.560.100.800.4592
 MUFA34.23ab36.10b33.67a0.593.060.0594
 PUFA43.38b41.37a43.76b0.553.370.0457
 n-32.8662.8012.9210.0700.680.5147
 n-640.43b38.49a40.76b0.493.700.0345
 n-6/n-314.1113.7513.960.190.780.4662
 PUFA/SFA1.938b1.835a1.939b0.0243.630.0367
 SFA/UFA0.2880.2910.2910.0020.800.4589
 AI0.3050.3100.3070.0030.780.4665
 TI0.4730.4790.4760.0030.970.3889
DietsSEMF-valueP-value
WBFCWBG
Chemical composition (g/100 g)
 Dry matter (DM)35.4234.4435.420.382.210.1243
 Ashes3.583.623.640.090.100.9029
 Ether extract32.3533.0632.560.560.410.6698
 Crude protein54.7153.8054.800.421.680.2014
Amino acid composition (g/100 g DM)
 Alanine4.012b4.098b3.916a0.0255.790.0066
 Arginine2.866b2.534a2.447a0.0764.520.0177
 Aspartic acid4.9684.7205.5700.3601.240.3014
 Cysteine0.5140.5340.5030.0290.300.7450
 Glutamic acid6.8156.8856.5830.1730.750.4785
 Glycine2.5932.6402.5000.0670.990.3830
 Histidine1.636b1.482a1.606ab0.0363.210.0520
 Isoleucine2.2972.2942.2560.0420.290.7506
 Leucine3.9133.8233.9040.0810.360.7002
 Lysine3.0322.9622.9690.0390.890.4213
 Methionine0.795a0.848b0.772ab0.0222.320.1128
 Phenylalanine1.930b1.822a1.907ab0.0253.260.0501
 Proline3.1773.1683.0800.0431.280.2893
 Serine2.6012.5292.8020.1730.620.5461
 Threonine2.120b1.812a1.798a0.0733.670.0354
 Tyrosine3.814b3.735a3.892c0.0168.040.0013
 Valine3.5513.5403.4940.0390.560.5762
Fatty acid composition (% total fatty acids)
 Lauric acid (C12:0)0.1310.1470.1310.0081.140.3319
 Myristic acid (C14:0)1.6781.7191.6600.0650.210.8152
 Pentadecanoic acid (C15:0)0.167b0.152a0.168b0.0035.460.0085
 Palmitic acid (C16:0)16.8216.9816.970.110.690.5103
 Palmitoleic acid (C16:1)1.124a1.220b1.112a0.0137.230.0023
 Heptadecanoic acid (C17:0)0.2140.2100.2280.0110.650.5257
 Cis-10-Heptadecenoic acid (C17:1)0.2210.1610.1820.0162.300.1140
 Stearic acid (C18:0)3.2703.2293.2980.0490.460.6351
 Elaidic acid (C18:1n9t)0.0450.0480.0440.0021.010.3731
 Oleic acid (C18:1n9c)32.59ab34.44b32.09a0.5812.990.0628
 Linoleic acid (C18:2n6c)40.28b38.35a40.62b0.493.730.0337
 Arachidic acid (C20:0)0.1100.0980.1070.0051.520.2323
 G-Linolenic acid (C18:3n6)0.0490.0510.0510.0030.150.8573
 Cis-11-Eicosenoic acid (C20:1)0.251b0.223a0.243b0.0037.570.0018
 Linolenic acid (C18:3n3)2.7712.7002.8250.0700.720.4955
 Cis-11,14-Eicosadienoic acid (C20:2)0.0880.0740.0820.0042.180.1280
 Cis-11,14,17-Eicosatrienoic acid (C20:3n3)0.0950.0970.1000.0030.550.5844
 Arachidonic acid (C20:4n6)0.0960.0860.0910.0050.910.4103
 SFA22.3822.5322.560.100.800.4592
 MUFA34.23ab36.10b33.67a0.593.060.0594
 PUFA43.38b41.37a43.76b0.553.370.0457
 n-32.8662.8012.9210.0700.680.5147
 n-640.43b38.49a40.76b0.493.700.0345
 n-6/n-314.1113.7513.960.190.780.4662
 PUFA/SFA1.938b1.835a1.939b0.0243.630.0367
 SFA/UFA0.2880.2910.2910.0020.800.4589
 AI0.3050.3100.3070.0030.780.4665
 TI0.4730.4790.4760.0030.970.3889

WB: diet including wheat bran and water; FC: diet including wheat bran and fresh carrots; WBG: diet including wheat bran, wet brewer’s grains and water (see Table 3). SEM: standard error of the means.

a,b,cMeans in the same row with no common superscripts differ significantly (P < 0.05). SFA,saturated fatty acids [C14:0 + C15:0 + C16:0 + C17:0 + C18:0]; MUFA, monounsaturated fatty acids; PUFA, polyunsaturated fatty acids; n-3: Omega-3 fatty acids; n-6: Omega-6 fatty acids; PUFA/SFA: ratio PUFA/SFA; SFA/UFA: ratio SFA/(MUFA + PUFA); AI, atherogenic index; TI, thrombogenic index.

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