| Plant Name . | Integration Plasmid . | Trait Gene(s) . | Marker Gene . | Type of Integration . | WT Locus . | attP (Empty Target) . | Genotype . | attB (Random Insertion) . | Bxb1 Integrase Gene . | Status of T0 Plants . |
|---|---|---|---|---|---|---|---|---|---|---|
| Primers n + i | Primers a + i | Primers c + b | Primers q + r | |||||||
| CTS3i1 | pGh10B | VdAL | bar-DsRed | I | – | + | Type I/attP | + | + | Plantlet |
| Primers o + d | Primers a + d | Primers c + e | Primers q + r | |||||||
| CTS4i4 | pGh10B | VdAL | bar-DsRed | I | − | + | Type I/attP | + | + | Plantlet |
| CTS4i1 | pGh1 | GbLTP, GbAt11 | bar | I + II | − | − | Type I/type II | + | + | Abnormal |
| CTS4i2 | pGh2 | bar-DsRed | I | + | − | Type I/WT | − | − | No seed | |
| CTS4i3 | pGh3 | DsRed-bar | I | + | − | Type I/WT | − | − | Abnormal | |
| pGh6 | VdAL, GbLTP, GbAt11 | hpt | Primers p + k | Primers a + k | Primers c + b | Primers q + r | ||||
| CTS1i1 | I | − | + | Type I/attP | − | − | Set bolls | |||
| CTS1i2 | I | − | + | Type I/attP | − | − | Set bolls | |||
| CTS1i3 | I | − | + | Type I/attP | − | − | Sacrificed for testing against verticillium wilt |
| Plant Name . | Integration Plasmid . | Trait Gene(s) . | Marker Gene . | Type of Integration . | WT Locus . | attP (Empty Target) . | Genotype . | attB (Random Insertion) . | Bxb1 Integrase Gene . | Status of T0 Plants . |
|---|---|---|---|---|---|---|---|---|---|---|
| Primers n + i | Primers a + i | Primers c + b | Primers q + r | |||||||
| CTS3i1 | pGh10B | VdAL | bar-DsRed | I | – | + | Type I/attP | + | + | Plantlet |
| Primers o + d | Primers a + d | Primers c + e | Primers q + r | |||||||
| CTS4i4 | pGh10B | VdAL | bar-DsRed | I | − | + | Type I/attP | + | + | Plantlet |
| CTS4i1 | pGh1 | GbLTP, GbAt11 | bar | I + II | − | − | Type I/type II | + | + | Abnormal |
| CTS4i2 | pGh2 | bar-DsRed | I | + | − | Type I/WT | − | − | No seed | |
| CTS4i3 | pGh3 | DsRed-bar | I | + | − | Type I/WT | − | − | Abnormal | |
| pGh6 | VdAL, GbLTP, GbAt11 | hpt | Primers p + k | Primers a + k | Primers c + b | Primers q + r | ||||
| CTS1i1 | I | − | + | Type I/attP | − | − | Set bolls | |||
| CTS1i2 | I | − | + | Type I/attP | − | − | Set bolls | |||
| CTS1i3 | I | − | + | Type I/attP | − | − | Sacrificed for testing against verticillium wilt |
“+” or “−” indicates the presence and absence of the specific recombination junctions.
| Plant Name . | Integration Plasmid . | Trait Gene(s) . | Marker Gene . | Type of Integration . | WT Locus . | attP (Empty Target) . | Genotype . | attB (Random Insertion) . | Bxb1 Integrase Gene . | Status of T0 Plants . |
|---|---|---|---|---|---|---|---|---|---|---|
| Primers n + i | Primers a + i | Primers c + b | Primers q + r | |||||||
| CTS3i1 | pGh10B | VdAL | bar-DsRed | I | – | + | Type I/attP | + | + | Plantlet |
| Primers o + d | Primers a + d | Primers c + e | Primers q + r | |||||||
| CTS4i4 | pGh10B | VdAL | bar-DsRed | I | − | + | Type I/attP | + | + | Plantlet |
| CTS4i1 | pGh1 | GbLTP, GbAt11 | bar | I + II | − | − | Type I/type II | + | + | Abnormal |
| CTS4i2 | pGh2 | bar-DsRed | I | + | − | Type I/WT | − | − | No seed | |
| CTS4i3 | pGh3 | DsRed-bar | I | + | − | Type I/WT | − | − | Abnormal | |
| pGh6 | VdAL, GbLTP, GbAt11 | hpt | Primers p + k | Primers a + k | Primers c + b | Primers q + r | ||||
| CTS1i1 | I | − | + | Type I/attP | − | − | Set bolls | |||
| CTS1i2 | I | − | + | Type I/attP | − | − | Set bolls | |||
| CTS1i3 | I | − | + | Type I/attP | − | − | Sacrificed for testing against verticillium wilt |
| Plant Name . | Integration Plasmid . | Trait Gene(s) . | Marker Gene . | Type of Integration . | WT Locus . | attP (Empty Target) . | Genotype . | attB (Random Insertion) . | Bxb1 Integrase Gene . | Status of T0 Plants . |
|---|---|---|---|---|---|---|---|---|---|---|
| Primers n + i | Primers a + i | Primers c + b | Primers q + r | |||||||
| CTS3i1 | pGh10B | VdAL | bar-DsRed | I | – | + | Type I/attP | + | + | Plantlet |
| Primers o + d | Primers a + d | Primers c + e | Primers q + r | |||||||
| CTS4i4 | pGh10B | VdAL | bar-DsRed | I | − | + | Type I/attP | + | + | Plantlet |
| CTS4i1 | pGh1 | GbLTP, GbAt11 | bar | I + II | − | − | Type I/type II | + | + | Abnormal |
| CTS4i2 | pGh2 | bar-DsRed | I | + | − | Type I/WT | − | − | No seed | |
| CTS4i3 | pGh3 | DsRed-bar | I | + | − | Type I/WT | − | − | Abnormal | |
| pGh6 | VdAL, GbLTP, GbAt11 | hpt | Primers p + k | Primers a + k | Primers c + b | Primers q + r | ||||
| CTS1i1 | I | − | + | Type I/attP | − | − | Set bolls | |||
| CTS1i2 | I | − | + | Type I/attP | − | − | Set bolls | |||
| CTS1i3 | I | − | + | Type I/attP | − | − | Sacrificed for testing against verticillium wilt |
“+” or “−” indicates the presence and absence of the specific recombination junctions.
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