| Target Line . | Integration Plasmid (size in kb) . | Number of Calluses Bombarded . | Calluses Positive for bar, bar+DsRed or hpt (Efficiency (%)) . | Number of PCR Positive Lines . | Recombination Junction Detected by PCR . | Site Specific Integration Efficiency . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| . | Type I . | Type II . | ||||||||
| Primers used | a + b | c + i | a + f | g + i | ||||||
| CTS3 | pGh10B (7.7) | 230 | 41 (18) | 1 | + | + | − | − | 9.8% (4/41) | |
| 3 | − | − | + | + | ||||||
| Primers used | a + b | c + d | a + f | g + d | ||||||
| CTS4 | pGh10B (7.7) | 30 | 13 (43) | 1 | + | + | − | − | 7.7% (1/13) | |
| Primers used | a + e | c + d | a + f | h + d | ||||||
| pGh1 (8) | 50 | 1 (2) | 1 | + | + | + | + | 100% (1/1) | ||
| pGh2 (8.7) | 250 | 13 (5.2) | 1 | + | + | − | − | 7.7% (1/13) | ||
| pGh3 (8.7) | 90 | 12 (13) | 1 | + | + | − | − | 8.3% (1/12) | ||
| Primers used | a + b | c + k | a + j | h + k | ||||||
| CTS1 | pGh6(11) | 200 | 15 (7.5) | 1 | + | + | − | − | 6.7% (1/15) | |
| Total | 850 | 95 (11) | 9.5% (9/95) | |||||||
| Target Line . | Integration Plasmid (size in kb) . | Number of Calluses Bombarded . | Calluses Positive for bar, bar+DsRed or hpt (Efficiency (%)) . | Number of PCR Positive Lines . | Recombination Junction Detected by PCR . | Site Specific Integration Efficiency . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| . | Type I . | Type II . | ||||||||
| Primers used | a + b | c + i | a + f | g + i | ||||||
| CTS3 | pGh10B (7.7) | 230 | 41 (18) | 1 | + | + | − | − | 9.8% (4/41) | |
| 3 | − | − | + | + | ||||||
| Primers used | a + b | c + d | a + f | g + d | ||||||
| CTS4 | pGh10B (7.7) | 30 | 13 (43) | 1 | + | + | − | − | 7.7% (1/13) | |
| Primers used | a + e | c + d | a + f | h + d | ||||||
| pGh1 (8) | 50 | 1 (2) | 1 | + | + | + | + | 100% (1/1) | ||
| pGh2 (8.7) | 250 | 13 (5.2) | 1 | + | + | − | − | 7.7% (1/13) | ||
| pGh3 (8.7) | 90 | 12 (13) | 1 | + | + | − | − | 8.3% (1/12) | ||
| Primers used | a + b | c + k | a + j | h + k | ||||||
| CTS1 | pGh6(11) | 200 | 15 (7.5) | 1 | + | + | − | − | 6.7% (1/15) | |
| Total | 850 | 95 (11) | 9.5% (9/95) | |||||||
“+” or “−” indicates the presence and absence of the specific recombination junctions.
| Target Line . | Integration Plasmid (size in kb) . | Number of Calluses Bombarded . | Calluses Positive for bar, bar+DsRed or hpt (Efficiency (%)) . | Number of PCR Positive Lines . | Recombination Junction Detected by PCR . | Site Specific Integration Efficiency . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| . | Type I . | Type II . | ||||||||
| Primers used | a + b | c + i | a + f | g + i | ||||||
| CTS3 | pGh10B (7.7) | 230 | 41 (18) | 1 | + | + | − | − | 9.8% (4/41) | |
| 3 | − | − | + | + | ||||||
| Primers used | a + b | c + d | a + f | g + d | ||||||
| CTS4 | pGh10B (7.7) | 30 | 13 (43) | 1 | + | + | − | − | 7.7% (1/13) | |
| Primers used | a + e | c + d | a + f | h + d | ||||||
| pGh1 (8) | 50 | 1 (2) | 1 | + | + | + | + | 100% (1/1) | ||
| pGh2 (8.7) | 250 | 13 (5.2) | 1 | + | + | − | − | 7.7% (1/13) | ||
| pGh3 (8.7) | 90 | 12 (13) | 1 | + | + | − | − | 8.3% (1/12) | ||
| Primers used | a + b | c + k | a + j | h + k | ||||||
| CTS1 | pGh6(11) | 200 | 15 (7.5) | 1 | + | + | − | − | 6.7% (1/15) | |
| Total | 850 | 95 (11) | 9.5% (9/95) | |||||||
| Target Line . | Integration Plasmid (size in kb) . | Number of Calluses Bombarded . | Calluses Positive for bar, bar+DsRed or hpt (Efficiency (%)) . | Number of PCR Positive Lines . | Recombination Junction Detected by PCR . | Site Specific Integration Efficiency . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| . | Type I . | Type II . | ||||||||
| Primers used | a + b | c + i | a + f | g + i | ||||||
| CTS3 | pGh10B (7.7) | 230 | 41 (18) | 1 | + | + | − | − | 9.8% (4/41) | |
| 3 | − | − | + | + | ||||||
| Primers used | a + b | c + d | a + f | g + d | ||||||
| CTS4 | pGh10B (7.7) | 30 | 13 (43) | 1 | + | + | − | − | 7.7% (1/13) | |
| Primers used | a + e | c + d | a + f | h + d | ||||||
| pGh1 (8) | 50 | 1 (2) | 1 | + | + | + | + | 100% (1/1) | ||
| pGh2 (8.7) | 250 | 13 (5.2) | 1 | + | + | − | − | 7.7% (1/13) | ||
| pGh3 (8.7) | 90 | 12 (13) | 1 | + | + | − | − | 8.3% (1/12) | ||
| Primers used | a + b | c + k | a + j | h + k | ||||||
| CTS1 | pGh6(11) | 200 | 15 (7.5) | 1 | + | + | − | − | 6.7% (1/15) | |
| Total | 850 | 95 (11) | 9.5% (9/95) | |||||||
“+” or “−” indicates the presence and absence of the specific recombination junctions.
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