Table 1. Open in new tab Summary of...

Population ID	Sample size	Heteroplasmic clone	Heteroplasmic sites	Minor allele frequencies
US D. pulex
BUS	88	59 (0.67)	2.75 [0.25]	0.116 [0.010]
CHQ	93	56 (0.60)	1.95 [0.29]	0.076 [0.011]
EB	77	29 (0.38)	8.86 [2.33]	0.068 [0.016]
KAP	79	65 (0.82)	2.80 [0.17]	0.067 [0.005]
LPA	87	59 (0.68)	4.29 [1.38]	0.078 [0.009]
LPB	84	56 (0.67)	7.54 [2.73]	0.085 [0.008]
NFL	89	63 (0.71)	2.91 [0.72]	0.094 [0.012]
PA	440	280 (0.64)	2.78 [0.26]	0.073 [0.004]
POV	64	47 (0.73)	6.70 [1.77]	0.073 [0.010]
TEX	66	50 (0.76)	12.18 [2.91]	0.072 [0.009]
Asex	36	33 (0.92)	21.55 [10.86]	0.053 [0.007]
OA (Oregon)	66	48 (0.73)	10.56 [5.09]	0.112 [0.012]
SH (Oregon)	90	66 (0.73)	2.30 [0.19]	0.087 [0.010]
Non-US D. pulex
BEL (Europe)	30	29 (0.97)	1.38 [0.11]	0.073 [0.009]
SZH (Asia)	42	42 (1.00)	22.23 [0.75]	0.177 [0.004]
D. pulicaria
BRA	79	73 (0.92)	2.18 [0.13]	0.033 [0.002]
CLO	60	60 (1.00)	13.37 [1.38]	0.046 [0.003]
TF	62	61 (0.98)	9.77 [0.90]	0.065 [0.010]
D. obtusa
EBG	67	66 (0.99)	27.03 [2.31]	0.054 [0.004]
PYR	43	43 (1.00)	28.74 [3.96]	0.055 [0.005]
RAP	62	62 (1.00)	302.00 [34.40]	0.049 [0.003]

Population ID

Sample size

Heteroplasmic clone

Heteroplasmic sites

Minor allele frequencies

US D. pulex

BUS

59 (0.67)

2.75 [0.25]

0.116 [0.010]

CHQ

56 (0.60)

1.95 [0.29]

0.076 [0.011]

29 (0.38)

8.86 [2.33]

0.068 [0.016]

KAP

65 (0.82)

2.80 [0.17]

0.067 [0.005]

LPA

59 (0.68)

4.29 [1.38]

0.078 [0.009]

LPB

56 (0.67)

7.54 [2.73]

0.085 [0.008]

NFL

63 (0.71)

2.91 [0.72]

0.094 [0.012]

440

280 (0.64)

2.78 [0.26]

0.073 [0.004]

POV

47 (0.73)

6.70 [1.77]

0.073 [0.010]

TEX

50 (0.76)

12.18 [2.91]

0.072 [0.009]

Asex

33 (0.92)

21.55 [10.86]

0.053 [0.007]

OA (Oregon)

48 (0.73)

10.56 [5.09]

0.112 [0.012]

SH (Oregon)

66 (0.73)

2.30 [0.19]

0.087 [0.010]

Non-US D. pulex

BEL (Europe)

29 (0.97)

1.38 [0.11]

0.073 [0.009]

SZH (Asia)

42 (1.00)

22.23 [0.75]

0.177 [0.004]

D. pulicaria

BRA

73 (0.92)

2.18 [0.13]

0.033 [0.002]

CLO

60 (1.00)

13.37 [1.38]

0.046 [0.003]

61 (0.98)

9.77 [0.90]

0.065 [0.010]

D. obtusa

EBG

66 (0.99)

27.03 [2.31]

0.054 [0.004]

PYR

43 (1.00)

28.74 [3.96]

0.055 [0.005]

RAP

62 (1.00)

302.00 [34.40]

0.049 [0.003]

Population ID	Sample size	Heteroplasmic clone	Heteroplasmic sites	Minor allele frequencies
US D. pulex
BUS	88	59 (0.67)	2.75 [0.25]	0.116 [0.010]
CHQ	93	56 (0.60)	1.95 [0.29]	0.076 [0.011]
EB	77	29 (0.38)	8.86 [2.33]	0.068 [0.016]
KAP	79	65 (0.82)	2.80 [0.17]	0.067 [0.005]
LPA	87	59 (0.68)	4.29 [1.38]	0.078 [0.009]
LPB	84	56 (0.67)	7.54 [2.73]	0.085 [0.008]
NFL	89	63 (0.71)	2.91 [0.72]	0.094 [0.012]
PA	440	280 (0.64)	2.78 [0.26]	0.073 [0.004]
POV	64	47 (0.73)	6.70 [1.77]	0.073 [0.010]
TEX	66	50 (0.76)	12.18 [2.91]	0.072 [0.009]
Asex	36	33 (0.92)	21.55 [10.86]	0.053 [0.007]
OA (Oregon)	66	48 (0.73)	10.56 [5.09]	0.112 [0.012]
SH (Oregon)	90	66 (0.73)	2.30 [0.19]	0.087 [0.010]
Non-US D. pulex
BEL (Europe)	30	29 (0.97)	1.38 [0.11]	0.073 [0.009]
SZH (Asia)	42	42 (1.00)	22.23 [0.75]	0.177 [0.004]
D. pulicaria
BRA	79	73 (0.92)	2.18 [0.13]	0.033 [0.002]
CLO	60	60 (1.00)	13.37 [1.38]	0.046 [0.003]
TF	62	61 (0.98)	9.77 [0.90]	0.065 [0.010]
D. obtusa
EBG	67	66 (0.99)	27.03 [2.31]	0.054 [0.004]
PYR	43	43 (1.00)	28.74 [3.96]	0.055 [0.005]
RAP	62	62 (1.00)	302.00 [34.40]	0.049 [0.003]

Table 1.

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Summary of Heteroplasmic Mutations for 1804 Clones from D. pulex, D. pulicaria, and D. obtusa.

Population ID	Sample size	Heteroplasmic clone	Heteroplasmic sites	Minor allele frequencies
US D. pulex
BUS	88	59 (0.67)	2.75 [0.25]	0.116 [0.010]
CHQ	93	56 (0.60)	1.95 [0.29]	0.076 [0.011]
EB	77	29 (0.38)	8.86 [2.33]	0.068 [0.016]
KAP	79	65 (0.82)	2.80 [0.17]	0.067 [0.005]
LPA	87	59 (0.68)	4.29 [1.38]	0.078 [0.009]
LPB	84	56 (0.67)	7.54 [2.73]	0.085 [0.008]
NFL	89	63 (0.71)	2.91 [0.72]	0.094 [0.012]
PA	440	280 (0.64)	2.78 [0.26]	0.073 [0.004]
POV	64	47 (0.73)	6.70 [1.77]	0.073 [0.010]
TEX	66	50 (0.76)	12.18 [2.91]	0.072 [0.009]
Asex	36	33 (0.92)	21.55 [10.86]	0.053 [0.007]
OA (Oregon)	66	48 (0.73)	10.56 [5.09]	0.112 [0.012]
SH (Oregon)	90	66 (0.73)	2.30 [0.19]	0.087 [0.010]
Non-US D. pulex
BEL (Europe)	30	29 (0.97)	1.38 [0.11]	0.073 [0.009]
SZH (Asia)	42	42 (1.00)	22.23 [0.75]	0.177 [0.004]
D. pulicaria
BRA	79	73 (0.92)	2.18 [0.13]	0.033 [0.002]
CLO	60	60 (1.00)	13.37 [1.38]	0.046 [0.003]
TF	62	61 (0.98)	9.77 [0.90]	0.065 [0.010]
D. obtusa
EBG	67	66 (0.99)	27.03 [2.31]	0.054 [0.004]
PYR	43	43 (1.00)	28.74 [3.96]	0.055 [0.005]
RAP	62	62 (1.00)	302.00 [34.40]	0.049 [0.003]

Population ID	Sample size	Heteroplasmic clone	Heteroplasmic sites	Minor allele frequencies
US D. pulex
BUS	88	59 (0.67)	2.75 [0.25]	0.116 [0.010]
CHQ	93	56 (0.60)	1.95 [0.29]	0.076 [0.011]
EB	77	29 (0.38)	8.86 [2.33]	0.068 [0.016]
KAP	79	65 (0.82)	2.80 [0.17]	0.067 [0.005]
LPA	87	59 (0.68)	4.29 [1.38]	0.078 [0.009]
LPB	84	56 (0.67)	7.54 [2.73]	0.085 [0.008]
NFL	89	63 (0.71)	2.91 [0.72]	0.094 [0.012]
PA	440	280 (0.64)	2.78 [0.26]	0.073 [0.004]
POV	64	47 (0.73)	6.70 [1.77]	0.073 [0.010]
TEX	66	50 (0.76)	12.18 [2.91]	0.072 [0.009]
Asex	36	33 (0.92)	21.55 [10.86]	0.053 [0.007]
OA (Oregon)	66	48 (0.73)	10.56 [5.09]	0.112 [0.012]
SH (Oregon)	90	66 (0.73)	2.30 [0.19]	0.087 [0.010]
Non-US D. pulex
BEL (Europe)	30	29 (0.97)	1.38 [0.11]	0.073 [0.009]
SZH (Asia)	42	42 (1.00)	22.23 [0.75]	0.177 [0.004]
D. pulicaria
BRA	79	73 (0.92)	2.18 [0.13]	0.033 [0.002]
CLO	60	60 (1.00)	13.37 [1.38]	0.046 [0.003]
TF	62	61 (0.98)	9.77 [0.90]	0.065 [0.010]
D. obtusa
EBG	67	66 (0.99)	27.03 [2.31]	0.054 [0.004]
PYR	43	43 (1.00)	28.74 [3.96]	0.055 [0.005]
RAP	62	62 (1.00)	302.00 [34.40]	0.049 [0.003]

Numbers within parentheses and brackets are fractions and standard errors. Mean minor-allele frequencies are calculated within each heteroplasmic clone and then averaged across all such clones.

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