Table 1. Open in new tab Various proK,...

Plasmids

Genotype

Description

pBMK72

proK^WT

Wild-type proK coding sequences.

pBMK73

proK^3M

3′ sequences downstream of CCA were changed from AA → CU (same as cysT).

pBMK74

proK^5M

C/G base pair at the 5′-mature terminus was changed to G/C base pair.

pBMK75

proK^5M+3M

The 5′-mature termini and 3′ downstream sequences of CCA were changed as described for pBMK74 and pBMK73, respectively.

pBMK77

proL^WT

Wild-type proL coding sequences.

pBMK78

proL^5M

C/G base pair at the 5′-mature terminus was changed to G/C base pair.

pBMK80

proL^3M

3′ sequences downstream of CCA were changed from AA → UU (same as hisR).

pBMK81

proL^5M+3M

The 5′-mature termini and 3′ downstream sequences of CCA were changed as described for pBMK78 and pBMK80, respectively.

pVMK208

pheV^WT

Wild-type pheV coding sequences.

pVMK210

pheV^3M

3′ sequences downstream of CCA were changed from CUAA → AAUU (same as proK).

pVMK212

pheV^5M+3M

The 5′-mature termini and 3′ downstream sequences of CCA were changed as described for pVMK214 and pVMK210, respectively.

pVMK214

pheV^5M

G/C base pair at the 5′-mature terminus was changed to C/G base pair.

Table 1.

Open in new tab

Various proK, proL and pheV plasmids used in the study. The proK, proL and pheV coding sequences without or with the indicated modifications were cloned with their native promoters and terminators in pWKS130, a 6–8 copy vector (19)

Plasmids	Genotype	Description
pBMK72	proK^WT	Wild-type proK coding sequences.
pBMK73	proK^3M	3′ sequences downstream of CCA were changed from AA → CU (same as cysT).
pBMK74	proK^5M	C/G base pair at the 5′-mature terminus was changed to G/C base pair.
pBMK75	proK^5M+3M	The 5′-mature termini and 3′ downstream sequences of CCA were changed as described for pBMK74 and pBMK73, respectively.
pBMK77	proL^WT	Wild-type proL coding sequences.
pBMK78	proL^5M	C/G base pair at the 5′-mature terminus was changed to G/C base pair.
pBMK80	proL^3M	3′ sequences downstream of CCA were changed from AA → UU (same as hisR).
pBMK81	proL^5M+3M	The 5′-mature termini and 3′ downstream sequences of CCA were changed as described for pBMK78 and pBMK80, respectively.
pVMK208	pheV^WT	Wild-type pheV coding sequences.
pVMK210	pheV^3M	3′ sequences downstream of CCA were changed from CUAA → AAUU (same as proK).
pVMK212	pheV^5M+3M	The 5′-mature termini and 3′ downstream sequences of CCA were changed as described for pVMK214 and pVMK210, respectively.
pVMK214	pheV^5M	G/C base pair at the 5′-mature terminus was changed to C/G base pair.

Plasmids	Genotype	Description
pBMK72	proK^WT	Wild-type proK coding sequences.
pBMK73	proK^3M	3′ sequences downstream of CCA were changed from AA → CU (same as cysT).
pBMK74	proK^5M	C/G base pair at the 5′-mature terminus was changed to G/C base pair.
pBMK75	proK^5M+3M	The 5′-mature termini and 3′ downstream sequences of CCA were changed as described for pBMK74 and pBMK73, respectively.
pBMK77	proL^WT	Wild-type proL coding sequences.
pBMK78	proL^5M	C/G base pair at the 5′-mature terminus was changed to G/C base pair.
pBMK80	proL^3M	3′ sequences downstream of CCA were changed from AA → UU (same as hisR).
pBMK81	proL^5M+3M	The 5′-mature termini and 3′ downstream sequences of CCA were changed as described for pBMK78 and pBMK80, respectively.
pVMK208	pheV^WT	Wild-type pheV coding sequences.
pVMK210	pheV^3M	3′ sequences downstream of CCA were changed from CUAA → AAUU (same as proK).
pVMK212	pheV^5M+3M	The 5′-mature termini and 3′ downstream sequences of CCA were changed as described for pVMK214 and pVMK210, respectively.
pVMK214	pheV^5M	G/C base pair at the 5′-mature terminus was changed to C/G base pair.

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