Novel MTases whose specificities were experimentally confirmed. The underlined characters indicate modified bases.
| Gene ID . | Genome ID . | Lineage . | Top-hit protein in REBASE . | Identity (%) . | RM type . | Recognition motif of the closest-match MTase . | Modification position . | Modification type . | RM system . | MTase name . | Confirmed recognition motif . | Novel specificity . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CM15mP129_7780 | CM1_5m.P129 | Bacteria; Chloroflexi; NA; NA; NA; NA; NA | M.Spn6BI | 35.5 | II | TCTAGA | (unknown) | (unknown) | No | M.CspCM15mP129I | ACAAA | Yes |
| Ct9H90mP5_10800 | Ct9H_90m.P5 | Bacteria; Actinobacteria; Acidimicrobiia; Acidimicrobiales; NA; NA; NA | M.Sgl8271II | 26.8 | II | ATTAAT | 5 | m6A | No | M.AspCt9H90mP5I | BAAAA | Yes |
| CM1200mP2_5150 | CM1_200m.P2 | Bacteria; Planctomycetes; Planctomycetia; Planctomycetales; Planctomycetaceae; NA; Planctomycetaceae bacterium | M.Sgl8271II | 24.5 | II | ATTAAT | 5 | m6A | No | M.PspCM1200mP2I | CAAAT | Yes |
| CM1200mP10_13750 | CM1_200m.P10 | Bacteria; Candidatus Marinimicrobia; NA; NA; NA; NA; Candidatus Marinimicrobia bacterium | M2.HpyAII | 44.2 | II | GAAGA | -2 | m4C | No | M.MspCM1200mP10I | CTCC | Yes |
| Ct9H300mP26_1870 | Ct9H_300m.P26 | Bacteria; Actinobacteria; Acidimicrobiia; Acidimicrobiales; NA; NA; NA | M.TliI | 25.0 | II | CTCGAG | 5 | m6A | No | M.AspCt9H300mP26I | BAAAA | Yes |
| CM15mP16_9820 | CM1_5m.P16 | Bacteria; Proteobacteria; Alphaproteobacteria; Pelagibacterales; NA; NA; NA | M.CspNS6I | 62.9 | II | GANTC | 2 | m6A | No | M.PspCM15mP16I | GANTC | No |
| CM15mP20_30 | CM1_5m.P20 | Bacteria; Proteobacteria; Alphaproteobacteria; NA; NA; NA; Alphaproteobacteria bacterium | M.SmeI | 59.9 | ? | GANTC | 2 | m6A | No | M.AspCM15mP20I | GADTC | Yes |
| CM15mP30_3110 | CM1_5m.P30 | Bacteria; Proteobacteria; Alphaproteobacteria; Pelagibacterales; Pelagibacteraceae; NA; Pelagibacteraceae bacterium | M.Bba35685I | 49.9 | II | GANTC | 2 | m6A | No | M.PspCM15mP30I | GAWTC | No |
| CM15mP57_4380 | CM1_5m.P57 | Bacteria; Proteobacteria; Alphaproteobacteria; NA; NA; NA; NA | M.SmeI | 57.0 | ? | GANTC | 2 | m6A | No | M.AspCM15mP57I | GAWTC | No |
| CM15mP70_4410 | CM1_5m.P70 | Bacteria; Proteobacteria; Alphaproteobacteria; Pelagibacterales; Pelagibacteraceae; NA; Alphaproteobacterium | M.Bsp460I | 48.0 | II | GANTC | 2 | m6A | No | M.PspCM15mP70I | GAWTC | No |
| CM15mP111_3240 | CM1_5m.P111 | Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; NA; NA; NA | M.SstE37II | 55.6 | II | GANTC | 2 | m6A | No | M.RspCM15mP111I | GAWTC | No |
| Gene ID . | Genome ID . | Lineage . | Top-hit protein in REBASE . | Identity (%) . | RM type . | Recognition motif of the closest-match MTase . | Modification position . | Modification type . | RM system . | MTase name . | Confirmed recognition motif . | Novel specificity . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CM15mP129_7780 | CM1_5m.P129 | Bacteria; Chloroflexi; NA; NA; NA; NA; NA | M.Spn6BI | 35.5 | II | TCTAGA | (unknown) | (unknown) | No | M.CspCM15mP129I | ACAAA | Yes |
| Ct9H90mP5_10800 | Ct9H_90m.P5 | Bacteria; Actinobacteria; Acidimicrobiia; Acidimicrobiales; NA; NA; NA | M.Sgl8271II | 26.8 | II | ATTAAT | 5 | m6A | No | M.AspCt9H90mP5I | BAAAA | Yes |
| CM1200mP2_5150 | CM1_200m.P2 | Bacteria; Planctomycetes; Planctomycetia; Planctomycetales; Planctomycetaceae; NA; Planctomycetaceae bacterium | M.Sgl8271II | 24.5 | II | ATTAAT | 5 | m6A | No | M.PspCM1200mP2I | CAAAT | Yes |
| CM1200mP10_13750 | CM1_200m.P10 | Bacteria; Candidatus Marinimicrobia; NA; NA; NA; NA; Candidatus Marinimicrobia bacterium | M2.HpyAII | 44.2 | II | GAAGA | -2 | m4C | No | M.MspCM1200mP10I | CTCC | Yes |
| Ct9H300mP26_1870 | Ct9H_300m.P26 | Bacteria; Actinobacteria; Acidimicrobiia; Acidimicrobiales; NA; NA; NA | M.TliI | 25.0 | II | CTCGAG | 5 | m6A | No | M.AspCt9H300mP26I | BAAAA | Yes |
| CM15mP16_9820 | CM1_5m.P16 | Bacteria; Proteobacteria; Alphaproteobacteria; Pelagibacterales; NA; NA; NA | M.CspNS6I | 62.9 | II | GANTC | 2 | m6A | No | M.PspCM15mP16I | GANTC | No |
| CM15mP20_30 | CM1_5m.P20 | Bacteria; Proteobacteria; Alphaproteobacteria; NA; NA; NA; Alphaproteobacteria bacterium | M.SmeI | 59.9 | ? | GANTC | 2 | m6A | No | M.AspCM15mP20I | GADTC | Yes |
| CM15mP30_3110 | CM1_5m.P30 | Bacteria; Proteobacteria; Alphaproteobacteria; Pelagibacterales; Pelagibacteraceae; NA; Pelagibacteraceae bacterium | M.Bba35685I | 49.9 | II | GANTC | 2 | m6A | No | M.PspCM15mP30I | GAWTC | No |
| CM15mP57_4380 | CM1_5m.P57 | Bacteria; Proteobacteria; Alphaproteobacteria; NA; NA; NA; NA | M.SmeI | 57.0 | ? | GANTC | 2 | m6A | No | M.AspCM15mP57I | GAWTC | No |
| CM15mP70_4410 | CM1_5m.P70 | Bacteria; Proteobacteria; Alphaproteobacteria; Pelagibacterales; Pelagibacteraceae; NA; Alphaproteobacterium | M.Bsp460I | 48.0 | II | GANTC | 2 | m6A | No | M.PspCM15mP70I | GAWTC | No |
| CM15mP111_3240 | CM1_5m.P111 | Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; NA; NA; NA | M.SstE37II | 55.6 | II | GANTC | 2 | m6A | No | M.RspCM15mP111I | GAWTC | No |
Novel MTases whose specificities were experimentally confirmed. The underlined characters indicate modified bases.
| Gene ID . | Genome ID . | Lineage . | Top-hit protein in REBASE . | Identity (%) . | RM type . | Recognition motif of the closest-match MTase . | Modification position . | Modification type . | RM system . | MTase name . | Confirmed recognition motif . | Novel specificity . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CM15mP129_7780 | CM1_5m.P129 | Bacteria; Chloroflexi; NA; NA; NA; NA; NA | M.Spn6BI | 35.5 | II | TCTAGA | (unknown) | (unknown) | No | M.CspCM15mP129I | ACAAA | Yes |
| Ct9H90mP5_10800 | Ct9H_90m.P5 | Bacteria; Actinobacteria; Acidimicrobiia; Acidimicrobiales; NA; NA; NA | M.Sgl8271II | 26.8 | II | ATTAAT | 5 | m6A | No | M.AspCt9H90mP5I | BAAAA | Yes |
| CM1200mP2_5150 | CM1_200m.P2 | Bacteria; Planctomycetes; Planctomycetia; Planctomycetales; Planctomycetaceae; NA; Planctomycetaceae bacterium | M.Sgl8271II | 24.5 | II | ATTAAT | 5 | m6A | No | M.PspCM1200mP2I | CAAAT | Yes |
| CM1200mP10_13750 | CM1_200m.P10 | Bacteria; Candidatus Marinimicrobia; NA; NA; NA; NA; Candidatus Marinimicrobia bacterium | M2.HpyAII | 44.2 | II | GAAGA | -2 | m4C | No | M.MspCM1200mP10I | CTCC | Yes |
| Ct9H300mP26_1870 | Ct9H_300m.P26 | Bacteria; Actinobacteria; Acidimicrobiia; Acidimicrobiales; NA; NA; NA | M.TliI | 25.0 | II | CTCGAG | 5 | m6A | No | M.AspCt9H300mP26I | BAAAA | Yes |
| CM15mP16_9820 | CM1_5m.P16 | Bacteria; Proteobacteria; Alphaproteobacteria; Pelagibacterales; NA; NA; NA | M.CspNS6I | 62.9 | II | GANTC | 2 | m6A | No | M.PspCM15mP16I | GANTC | No |
| CM15mP20_30 | CM1_5m.P20 | Bacteria; Proteobacteria; Alphaproteobacteria; NA; NA; NA; Alphaproteobacteria bacterium | M.SmeI | 59.9 | ? | GANTC | 2 | m6A | No | M.AspCM15mP20I | GADTC | Yes |
| CM15mP30_3110 | CM1_5m.P30 | Bacteria; Proteobacteria; Alphaproteobacteria; Pelagibacterales; Pelagibacteraceae; NA; Pelagibacteraceae bacterium | M.Bba35685I | 49.9 | II | GANTC | 2 | m6A | No | M.PspCM15mP30I | GAWTC | No |
| CM15mP57_4380 | CM1_5m.P57 | Bacteria; Proteobacteria; Alphaproteobacteria; NA; NA; NA; NA | M.SmeI | 57.0 | ? | GANTC | 2 | m6A | No | M.AspCM15mP57I | GAWTC | No |
| CM15mP70_4410 | CM1_5m.P70 | Bacteria; Proteobacteria; Alphaproteobacteria; Pelagibacterales; Pelagibacteraceae; NA; Alphaproteobacterium | M.Bsp460I | 48.0 | II | GANTC | 2 | m6A | No | M.PspCM15mP70I | GAWTC | No |
| CM15mP111_3240 | CM1_5m.P111 | Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; NA; NA; NA | M.SstE37II | 55.6 | II | GANTC | 2 | m6A | No | M.RspCM15mP111I | GAWTC | No |
| Gene ID . | Genome ID . | Lineage . | Top-hit protein in REBASE . | Identity (%) . | RM type . | Recognition motif of the closest-match MTase . | Modification position . | Modification type . | RM system . | MTase name . | Confirmed recognition motif . | Novel specificity . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CM15mP129_7780 | CM1_5m.P129 | Bacteria; Chloroflexi; NA; NA; NA; NA; NA | M.Spn6BI | 35.5 | II | TCTAGA | (unknown) | (unknown) | No | M.CspCM15mP129I | ACAAA | Yes |
| Ct9H90mP5_10800 | Ct9H_90m.P5 | Bacteria; Actinobacteria; Acidimicrobiia; Acidimicrobiales; NA; NA; NA | M.Sgl8271II | 26.8 | II | ATTAAT | 5 | m6A | No | M.AspCt9H90mP5I | BAAAA | Yes |
| CM1200mP2_5150 | CM1_200m.P2 | Bacteria; Planctomycetes; Planctomycetia; Planctomycetales; Planctomycetaceae; NA; Planctomycetaceae bacterium | M.Sgl8271II | 24.5 | II | ATTAAT | 5 | m6A | No | M.PspCM1200mP2I | CAAAT | Yes |
| CM1200mP10_13750 | CM1_200m.P10 | Bacteria; Candidatus Marinimicrobia; NA; NA; NA; NA; Candidatus Marinimicrobia bacterium | M2.HpyAII | 44.2 | II | GAAGA | -2 | m4C | No | M.MspCM1200mP10I | CTCC | Yes |
| Ct9H300mP26_1870 | Ct9H_300m.P26 | Bacteria; Actinobacteria; Acidimicrobiia; Acidimicrobiales; NA; NA; NA | M.TliI | 25.0 | II | CTCGAG | 5 | m6A | No | M.AspCt9H300mP26I | BAAAA | Yes |
| CM15mP16_9820 | CM1_5m.P16 | Bacteria; Proteobacteria; Alphaproteobacteria; Pelagibacterales; NA; NA; NA | M.CspNS6I | 62.9 | II | GANTC | 2 | m6A | No | M.PspCM15mP16I | GANTC | No |
| CM15mP20_30 | CM1_5m.P20 | Bacteria; Proteobacteria; Alphaproteobacteria; NA; NA; NA; Alphaproteobacteria bacterium | M.SmeI | 59.9 | ? | GANTC | 2 | m6A | No | M.AspCM15mP20I | GADTC | Yes |
| CM15mP30_3110 | CM1_5m.P30 | Bacteria; Proteobacteria; Alphaproteobacteria; Pelagibacterales; Pelagibacteraceae; NA; Pelagibacteraceae bacterium | M.Bba35685I | 49.9 | II | GANTC | 2 | m6A | No | M.PspCM15mP30I | GAWTC | No |
| CM15mP57_4380 | CM1_5m.P57 | Bacteria; Proteobacteria; Alphaproteobacteria; NA; NA; NA; NA | M.SmeI | 57.0 | ? | GANTC | 2 | m6A | No | M.AspCM15mP57I | GAWTC | No |
| CM15mP70_4410 | CM1_5m.P70 | Bacteria; Proteobacteria; Alphaproteobacteria; Pelagibacterales; Pelagibacteraceae; NA; Alphaproteobacterium | M.Bsp460I | 48.0 | II | GANTC | 2 | m6A | No | M.PspCM15mP70I | GAWTC | No |
| CM15mP111_3240 | CM1_5m.P111 | Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; NA; NA; NA | M.SstE37II | 55.6 | II | GANTC | 2 | m6A | No | M.RspCM15mP111I | GAWTC | No |
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