Primers Used in the Amplification and Sequencing of the Complete Mitochondrial Genome of Lampsilis ornata.
| Primer . | Primer Sequence (5′ to 3′) . | Base Position Range . |
|---|---|---|
| ND4-1084R | GGAGCTGCCATGTTCATTGATCTTA | 263–287 |
| aND1-end | ATAGCACACATTAAAAACCAACACAAAGC | 278–306 |
| ND4-630F | TCGCCAAAGCTCATGTTGAGG | 705–725 |
| aAsp-end | GGTGCTTATTTTTTCTTTTAGGGTTGGTTC | 1013–1042 |
| aAsp-196F | GAAGCAGCGTTAGGGTTGAGTGTATTAG | 1402–1429 |
| aAsp-150Fc | GTGAAGTGGGGTTTTGGTAAGAAGTTTAG | 1668–1696 |
| bAsp-54F | GCAGGCTTATGTGTTTGTTTTGTTGG | 1972–1997 |
| aCO3-350F | CTGTCTTTGACTTTTGTGTTGGCTAG | 2310–2335 |
| aCO3-60F | TCATCTTTGGATGCTGGGTTAAGCTA | 2592–2617 |
| CO3-730R | TCAAACTACATCTACAAAATGTCAG | 2724–2748 |
| CO3-312F | GTATGGGTTAGGTTTAACGGTCTTATTGG | 2963–2991 |
| CO3-176R | CACAACCCAACTCCACAGTTGGAACAAG | 3128–3155 |
| CO3-180F | TGGTGGCGAGATGTAATTCGTGAAG | 3278–3302 |
| CO3-60R | GGTCTTTCCTGCGTCATTTCACTCG | 3528–3552 |
| bCO3-486R | GTTAGACAATACAATACCCGTCAATCC | 3963–3989 |
| HCO2189a | TTAACTTCAGGGTGACCAAAAAATCA | 4312–4338 |
| COI-477F | GATTCCGTTGTTTGTATGGGCTGTTACTG | 4475–4503 |
| J2160-F | CTTTACATCTTGCTGGTGCCTCTTCTAT | 4570–4597 |
| COI-215R | CCATATCAGGAGCCCCAATCATAAGAG | 4766–4792 |
| LCO1490a | GGTCAACAAATCATAAAGATATTGG | 4996–5020 |
| J408-R | TTGGGCTCTTCCTTCAGTCGGTGT | 5224–5247 |
| J190-R | CCTNACAAGAACTCGCCTATAAACCCCA | 5268–5295 |
| aHis-580R | ATTTGTTCTTGTCTTGGTTTCGTCTTTTG | 5624–5652 |
| J420/190R | GCCACTCATGAATAACCCCACCAAA | 5841–5865 |
| Ala-F | GCAATCAACCATTGAGTCCAACTCTAGAAC | 6460–6489 |
| aAla-540F | GTCCTATCTTCCTCAAACTGATTAACAACC | 7086–7115 |
| Met-R | GAAGAGTGATCATTTTGGGTTATGAGCC | 8023–8052 |
| L1091b | TAATAAAAAACTAGGATTAGATACCCTATTAT | 8603–8636 |
| L1091-R | GTAGTCGGGTATCTAATCCGAGTTTTGAGGTTGT | 8603–8638 |
| 12S-100R | GATGAGGAAGTAAAGCACCGCCACA | 8689–8713 |
| 12S-360F | TTAGCACACCAAGCAAATCTGAACAAGAACT | 8952–8982 |
| H1478b | TAATAATAAGAGCGACGGGCGATGTGT | 8992–9019 |
| 16S-70R | CTGTGGTGGGCGTAGAGGTAGTGAC | 9369–9393 |
| 16Sar-L-mytc | CGACTGTTTAACAAAAACAT | 9925–9943 |
| 16S-780F | CTAAATAATAAAGGAAGACGAAAAGACC | 10127–10154 |
| 16S-78R | CGGGGTCTTTTCGTCTTCCTTTATTAT | 10131–10157 |
| 16Sbr-H-mytc | CCGTTCTGAACTCAGCTCATGT | 10432–10452 |
| Pro-10R | CTATTTTCTGGGGTGAGTATGTGTAGG | 10747–10773 |
| unicytbFd | TGTGGGGCGACTGTTATTACTAA | 11253–11275 |
| cytB-150R | GCAATAGCAAAAGGAAGGACAAAGTGAAG | 11373–11401 |
| cytB-390F | GTACTAACTCCCACTTGCTTGTTCTTCC | 11544–11571 |
| unicytbRd | AAGAAGTATCATTCGGGTTG | 11637–11656 |
| Phe-47F | GCCATTGCCCTTCTCCATTTTCCCATAC | 12034–12061 |
| aPhe-334F | CAATACCAGAAATCAACCTTCCAGACAC | 12387–12414 |
| aPhe-768Fc | TACACTCCTTAGCACCAAACACCCAC | 12871–12896 |
| bGln-210Rc | CTACTCTTGTTGAGTGTAGATCACATTGC | 13942–13970 |
| ND1-350R | GGTTGTTGGAAATAAAGGTAGATGGGGTAAGAT | 14015–14045 |
| Gln-47R | GCAAATTGGTTGAAAGCGGAAATATTGG | 14220–14249 |
| ND1-110R | AGGTAGATAGTTTGAAGAGGTTGGTGTTA | 14707–14735 |
| ND1-240F | CTAGCCGTCTACACAACCCTCATAACAG | 14856–14883 |
| NIJ-12073e | TCGGATTCTCCTTCTGCAAAGTC | 15120–15142 |
| ND1-1750F | TACCATAATCTAACCTCAACTCTAAACA | 15410–15437 |
| aND1-385F | ACTCTCCCTCAAACTATCAAACAAATC | 15823–15849 |
| Primer . | Primer Sequence (5′ to 3′) . | Base Position Range . |
|---|---|---|
| ND4-1084R | GGAGCTGCCATGTTCATTGATCTTA | 263–287 |
| aND1-end | ATAGCACACATTAAAAACCAACACAAAGC | 278–306 |
| ND4-630F | TCGCCAAAGCTCATGTTGAGG | 705–725 |
| aAsp-end | GGTGCTTATTTTTTCTTTTAGGGTTGGTTC | 1013–1042 |
| aAsp-196F | GAAGCAGCGTTAGGGTTGAGTGTATTAG | 1402–1429 |
| aAsp-150Fc | GTGAAGTGGGGTTTTGGTAAGAAGTTTAG | 1668–1696 |
| bAsp-54F | GCAGGCTTATGTGTTTGTTTTGTTGG | 1972–1997 |
| aCO3-350F | CTGTCTTTGACTTTTGTGTTGGCTAG | 2310–2335 |
| aCO3-60F | TCATCTTTGGATGCTGGGTTAAGCTA | 2592–2617 |
| CO3-730R | TCAAACTACATCTACAAAATGTCAG | 2724–2748 |
| CO3-312F | GTATGGGTTAGGTTTAACGGTCTTATTGG | 2963–2991 |
| CO3-176R | CACAACCCAACTCCACAGTTGGAACAAG | 3128–3155 |
| CO3-180F | TGGTGGCGAGATGTAATTCGTGAAG | 3278–3302 |
| CO3-60R | GGTCTTTCCTGCGTCATTTCACTCG | 3528–3552 |
| bCO3-486R | GTTAGACAATACAATACCCGTCAATCC | 3963–3989 |
| HCO2189a | TTAACTTCAGGGTGACCAAAAAATCA | 4312–4338 |
| COI-477F | GATTCCGTTGTTTGTATGGGCTGTTACTG | 4475–4503 |
| J2160-F | CTTTACATCTTGCTGGTGCCTCTTCTAT | 4570–4597 |
| COI-215R | CCATATCAGGAGCCCCAATCATAAGAG | 4766–4792 |
| LCO1490a | GGTCAACAAATCATAAAGATATTGG | 4996–5020 |
| J408-R | TTGGGCTCTTCCTTCAGTCGGTGT | 5224–5247 |
| J190-R | CCTNACAAGAACTCGCCTATAAACCCCA | 5268–5295 |
| aHis-580R | ATTTGTTCTTGTCTTGGTTTCGTCTTTTG | 5624–5652 |
| J420/190R | GCCACTCATGAATAACCCCACCAAA | 5841–5865 |
| Ala-F | GCAATCAACCATTGAGTCCAACTCTAGAAC | 6460–6489 |
| aAla-540F | GTCCTATCTTCCTCAAACTGATTAACAACC | 7086–7115 |
| Met-R | GAAGAGTGATCATTTTGGGTTATGAGCC | 8023–8052 |
| L1091b | TAATAAAAAACTAGGATTAGATACCCTATTAT | 8603–8636 |
| L1091-R | GTAGTCGGGTATCTAATCCGAGTTTTGAGGTTGT | 8603–8638 |
| 12S-100R | GATGAGGAAGTAAAGCACCGCCACA | 8689–8713 |
| 12S-360F | TTAGCACACCAAGCAAATCTGAACAAGAACT | 8952–8982 |
| H1478b | TAATAATAAGAGCGACGGGCGATGTGT | 8992–9019 |
| 16S-70R | CTGTGGTGGGCGTAGAGGTAGTGAC | 9369–9393 |
| 16Sar-L-mytc | CGACTGTTTAACAAAAACAT | 9925–9943 |
| 16S-780F | CTAAATAATAAAGGAAGACGAAAAGACC | 10127–10154 |
| 16S-78R | CGGGGTCTTTTCGTCTTCCTTTATTAT | 10131–10157 |
| 16Sbr-H-mytc | CCGTTCTGAACTCAGCTCATGT | 10432–10452 |
| Pro-10R | CTATTTTCTGGGGTGAGTATGTGTAGG | 10747–10773 |
| unicytbFd | TGTGGGGCGACTGTTATTACTAA | 11253–11275 |
| cytB-150R | GCAATAGCAAAAGGAAGGACAAAGTGAAG | 11373–11401 |
| cytB-390F | GTACTAACTCCCACTTGCTTGTTCTTCC | 11544–11571 |
| unicytbRd | AAGAAGTATCATTCGGGTTG | 11637–11656 |
| Phe-47F | GCCATTGCCCTTCTCCATTTTCCCATAC | 12034–12061 |
| aPhe-334F | CAATACCAGAAATCAACCTTCCAGACAC | 12387–12414 |
| aPhe-768Fc | TACACTCCTTAGCACCAAACACCCAC | 12871–12896 |
| bGln-210Rc | CTACTCTTGTTGAGTGTAGATCACATTGC | 13942–13970 |
| ND1-350R | GGTTGTTGGAAATAAAGGTAGATGGGGTAAGAT | 14015–14045 |
| Gln-47R | GCAAATTGGTTGAAAGCGGAAATATTGG | 14220–14249 |
| ND1-110R | AGGTAGATAGTTTGAAGAGGTTGGTGTTA | 14707–14735 |
| ND1-240F | CTAGCCGTCTACACAACCCTCATAACAG | 14856–14883 |
| NIJ-12073e | TCGGATTCTCCTTCTGCAAAGTC | 15120–15142 |
| ND1-1750F | TACCATAATCTAACCTCAACTCTAAACA | 15410–15437 |
| aND1-385F | ACTCTCCCTCAAACTATCAAACAAATC | 15823–15849 |
aFolmer et al. 1994.
bWhite, Mcpheron, and Stauffer 1996.
cLydeard, Mulvey, and Davis 1996.
dBonnie Bowen, unpublished data.
eSerb, Buhay, and Lydeard 2003.
Primers Used in the Amplification and Sequencing of the Complete Mitochondrial Genome of Lampsilis ornata.
| Primer . | Primer Sequence (5′ to 3′) . | Base Position Range . |
|---|---|---|
| ND4-1084R | GGAGCTGCCATGTTCATTGATCTTA | 263–287 |
| aND1-end | ATAGCACACATTAAAAACCAACACAAAGC | 278–306 |
| ND4-630F | TCGCCAAAGCTCATGTTGAGG | 705–725 |
| aAsp-end | GGTGCTTATTTTTTCTTTTAGGGTTGGTTC | 1013–1042 |
| aAsp-196F | GAAGCAGCGTTAGGGTTGAGTGTATTAG | 1402–1429 |
| aAsp-150Fc | GTGAAGTGGGGTTTTGGTAAGAAGTTTAG | 1668–1696 |
| bAsp-54F | GCAGGCTTATGTGTTTGTTTTGTTGG | 1972–1997 |
| aCO3-350F | CTGTCTTTGACTTTTGTGTTGGCTAG | 2310–2335 |
| aCO3-60F | TCATCTTTGGATGCTGGGTTAAGCTA | 2592–2617 |
| CO3-730R | TCAAACTACATCTACAAAATGTCAG | 2724–2748 |
| CO3-312F | GTATGGGTTAGGTTTAACGGTCTTATTGG | 2963–2991 |
| CO3-176R | CACAACCCAACTCCACAGTTGGAACAAG | 3128–3155 |
| CO3-180F | TGGTGGCGAGATGTAATTCGTGAAG | 3278–3302 |
| CO3-60R | GGTCTTTCCTGCGTCATTTCACTCG | 3528–3552 |
| bCO3-486R | GTTAGACAATACAATACCCGTCAATCC | 3963–3989 |
| HCO2189a | TTAACTTCAGGGTGACCAAAAAATCA | 4312–4338 |
| COI-477F | GATTCCGTTGTTTGTATGGGCTGTTACTG | 4475–4503 |
| J2160-F | CTTTACATCTTGCTGGTGCCTCTTCTAT | 4570–4597 |
| COI-215R | CCATATCAGGAGCCCCAATCATAAGAG | 4766–4792 |
| LCO1490a | GGTCAACAAATCATAAAGATATTGG | 4996–5020 |
| J408-R | TTGGGCTCTTCCTTCAGTCGGTGT | 5224–5247 |
| J190-R | CCTNACAAGAACTCGCCTATAAACCCCA | 5268–5295 |
| aHis-580R | ATTTGTTCTTGTCTTGGTTTCGTCTTTTG | 5624–5652 |
| J420/190R | GCCACTCATGAATAACCCCACCAAA | 5841–5865 |
| Ala-F | GCAATCAACCATTGAGTCCAACTCTAGAAC | 6460–6489 |
| aAla-540F | GTCCTATCTTCCTCAAACTGATTAACAACC | 7086–7115 |
| Met-R | GAAGAGTGATCATTTTGGGTTATGAGCC | 8023–8052 |
| L1091b | TAATAAAAAACTAGGATTAGATACCCTATTAT | 8603–8636 |
| L1091-R | GTAGTCGGGTATCTAATCCGAGTTTTGAGGTTGT | 8603–8638 |
| 12S-100R | GATGAGGAAGTAAAGCACCGCCACA | 8689–8713 |
| 12S-360F | TTAGCACACCAAGCAAATCTGAACAAGAACT | 8952–8982 |
| H1478b | TAATAATAAGAGCGACGGGCGATGTGT | 8992–9019 |
| 16S-70R | CTGTGGTGGGCGTAGAGGTAGTGAC | 9369–9393 |
| 16Sar-L-mytc | CGACTGTTTAACAAAAACAT | 9925–9943 |
| 16S-780F | CTAAATAATAAAGGAAGACGAAAAGACC | 10127–10154 |
| 16S-78R | CGGGGTCTTTTCGTCTTCCTTTATTAT | 10131–10157 |
| 16Sbr-H-mytc | CCGTTCTGAACTCAGCTCATGT | 10432–10452 |
| Pro-10R | CTATTTTCTGGGGTGAGTATGTGTAGG | 10747–10773 |
| unicytbFd | TGTGGGGCGACTGTTATTACTAA | 11253–11275 |
| cytB-150R | GCAATAGCAAAAGGAAGGACAAAGTGAAG | 11373–11401 |
| cytB-390F | GTACTAACTCCCACTTGCTTGTTCTTCC | 11544–11571 |
| unicytbRd | AAGAAGTATCATTCGGGTTG | 11637–11656 |
| Phe-47F | GCCATTGCCCTTCTCCATTTTCCCATAC | 12034–12061 |
| aPhe-334F | CAATACCAGAAATCAACCTTCCAGACAC | 12387–12414 |
| aPhe-768Fc | TACACTCCTTAGCACCAAACACCCAC | 12871–12896 |
| bGln-210Rc | CTACTCTTGTTGAGTGTAGATCACATTGC | 13942–13970 |
| ND1-350R | GGTTGTTGGAAATAAAGGTAGATGGGGTAAGAT | 14015–14045 |
| Gln-47R | GCAAATTGGTTGAAAGCGGAAATATTGG | 14220–14249 |
| ND1-110R | AGGTAGATAGTTTGAAGAGGTTGGTGTTA | 14707–14735 |
| ND1-240F | CTAGCCGTCTACACAACCCTCATAACAG | 14856–14883 |
| NIJ-12073e | TCGGATTCTCCTTCTGCAAAGTC | 15120–15142 |
| ND1-1750F | TACCATAATCTAACCTCAACTCTAAACA | 15410–15437 |
| aND1-385F | ACTCTCCCTCAAACTATCAAACAAATC | 15823–15849 |
| Primer . | Primer Sequence (5′ to 3′) . | Base Position Range . |
|---|---|---|
| ND4-1084R | GGAGCTGCCATGTTCATTGATCTTA | 263–287 |
| aND1-end | ATAGCACACATTAAAAACCAACACAAAGC | 278–306 |
| ND4-630F | TCGCCAAAGCTCATGTTGAGG | 705–725 |
| aAsp-end | GGTGCTTATTTTTTCTTTTAGGGTTGGTTC | 1013–1042 |
| aAsp-196F | GAAGCAGCGTTAGGGTTGAGTGTATTAG | 1402–1429 |
| aAsp-150Fc | GTGAAGTGGGGTTTTGGTAAGAAGTTTAG | 1668–1696 |
| bAsp-54F | GCAGGCTTATGTGTTTGTTTTGTTGG | 1972–1997 |
| aCO3-350F | CTGTCTTTGACTTTTGTGTTGGCTAG | 2310–2335 |
| aCO3-60F | TCATCTTTGGATGCTGGGTTAAGCTA | 2592–2617 |
| CO3-730R | TCAAACTACATCTACAAAATGTCAG | 2724–2748 |
| CO3-312F | GTATGGGTTAGGTTTAACGGTCTTATTGG | 2963–2991 |
| CO3-176R | CACAACCCAACTCCACAGTTGGAACAAG | 3128–3155 |
| CO3-180F | TGGTGGCGAGATGTAATTCGTGAAG | 3278–3302 |
| CO3-60R | GGTCTTTCCTGCGTCATTTCACTCG | 3528–3552 |
| bCO3-486R | GTTAGACAATACAATACCCGTCAATCC | 3963–3989 |
| HCO2189a | TTAACTTCAGGGTGACCAAAAAATCA | 4312–4338 |
| COI-477F | GATTCCGTTGTTTGTATGGGCTGTTACTG | 4475–4503 |
| J2160-F | CTTTACATCTTGCTGGTGCCTCTTCTAT | 4570–4597 |
| COI-215R | CCATATCAGGAGCCCCAATCATAAGAG | 4766–4792 |
| LCO1490a | GGTCAACAAATCATAAAGATATTGG | 4996–5020 |
| J408-R | TTGGGCTCTTCCTTCAGTCGGTGT | 5224–5247 |
| J190-R | CCTNACAAGAACTCGCCTATAAACCCCA | 5268–5295 |
| aHis-580R | ATTTGTTCTTGTCTTGGTTTCGTCTTTTG | 5624–5652 |
| J420/190R | GCCACTCATGAATAACCCCACCAAA | 5841–5865 |
| Ala-F | GCAATCAACCATTGAGTCCAACTCTAGAAC | 6460–6489 |
| aAla-540F | GTCCTATCTTCCTCAAACTGATTAACAACC | 7086–7115 |
| Met-R | GAAGAGTGATCATTTTGGGTTATGAGCC | 8023–8052 |
| L1091b | TAATAAAAAACTAGGATTAGATACCCTATTAT | 8603–8636 |
| L1091-R | GTAGTCGGGTATCTAATCCGAGTTTTGAGGTTGT | 8603–8638 |
| 12S-100R | GATGAGGAAGTAAAGCACCGCCACA | 8689–8713 |
| 12S-360F | TTAGCACACCAAGCAAATCTGAACAAGAACT | 8952–8982 |
| H1478b | TAATAATAAGAGCGACGGGCGATGTGT | 8992–9019 |
| 16S-70R | CTGTGGTGGGCGTAGAGGTAGTGAC | 9369–9393 |
| 16Sar-L-mytc | CGACTGTTTAACAAAAACAT | 9925–9943 |
| 16S-780F | CTAAATAATAAAGGAAGACGAAAAGACC | 10127–10154 |
| 16S-78R | CGGGGTCTTTTCGTCTTCCTTTATTAT | 10131–10157 |
| 16Sbr-H-mytc | CCGTTCTGAACTCAGCTCATGT | 10432–10452 |
| Pro-10R | CTATTTTCTGGGGTGAGTATGTGTAGG | 10747–10773 |
| unicytbFd | TGTGGGGCGACTGTTATTACTAA | 11253–11275 |
| cytB-150R | GCAATAGCAAAAGGAAGGACAAAGTGAAG | 11373–11401 |
| cytB-390F | GTACTAACTCCCACTTGCTTGTTCTTCC | 11544–11571 |
| unicytbRd | AAGAAGTATCATTCGGGTTG | 11637–11656 |
| Phe-47F | GCCATTGCCCTTCTCCATTTTCCCATAC | 12034–12061 |
| aPhe-334F | CAATACCAGAAATCAACCTTCCAGACAC | 12387–12414 |
| aPhe-768Fc | TACACTCCTTAGCACCAAACACCCAC | 12871–12896 |
| bGln-210Rc | CTACTCTTGTTGAGTGTAGATCACATTGC | 13942–13970 |
| ND1-350R | GGTTGTTGGAAATAAAGGTAGATGGGGTAAGAT | 14015–14045 |
| Gln-47R | GCAAATTGGTTGAAAGCGGAAATATTGG | 14220–14249 |
| ND1-110R | AGGTAGATAGTTTGAAGAGGTTGGTGTTA | 14707–14735 |
| ND1-240F | CTAGCCGTCTACACAACCCTCATAACAG | 14856–14883 |
| NIJ-12073e | TCGGATTCTCCTTCTGCAAAGTC | 15120–15142 |
| ND1-1750F | TACCATAATCTAACCTCAACTCTAAACA | 15410–15437 |
| aND1-385F | ACTCTCCCTCAAACTATCAAACAAATC | 15823–15849 |
aFolmer et al. 1994.
bWhite, Mcpheron, and Stauffer 1996.
cLydeard, Mulvey, and Davis 1996.
dBonnie Bowen, unpublished data.
eSerb, Buhay, and Lydeard 2003.
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