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Table 1

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Purification of CarS (CtPOD1) from the crude extracts of the corollas of the cream cultivar

	Total protein	Total activity	Specific activity	Purification	Recovery
Step	(mg)	(nkat)	(nkat/mg protein)	(-fold)	(%)
1. Crude extract	607	136	0.224	1	100
2. DEAE Sephadex	208	116	0.558	2.5	84.9
3. SP Sepharose FF	143	74.9	0.525	2.3	55
4. Ammonium sulfate fractionation	56.5	13.8	0.246	1.1	10.2
5. CM Sepharose FF	1.8	5.3	2.93	13	3.9
6. Bio-Scale CHT2-1	0.31	2.76	8.92	39.8	2
7. Superdex 200	0.00559	1.59	284	1,267	1.2

	Total protein	Total activity	Specific activity	Purification	Recovery
Step	(mg)	(nkat)	(nkat/mg protein)	(-fold)	(%)
1. Crude extract	607	136	0.224	1	100
2. DEAE Sephadex	208	116	0.558	2.5	84.9
3. SP Sepharose FF	143	74.9	0.525	2.3	55
4. Ammonium sulfate fractionation	56.5	13.8	0.246	1.1	10.2
5. CM Sepharose FF	1.8	5.3	2.93	13	3.9
6. Bio-Scale CHT2-1	0.31	2.76	8.92	39.8	2
7. Superdex 200	0.00559	1.59	284	1,267	1.2

Table 1

Open in new tab

Purification of CarS (CtPOD1) from the crude extracts of the corollas of the cream cultivar

	Total protein	Total activity	Specific activity	Purification	Recovery
Step	(mg)	(nkat)	(nkat/mg protein)	(-fold)	(%)
1. Crude extract	607	136	0.224	1	100
2. DEAE Sephadex	208	116	0.558	2.5	84.9
3. SP Sepharose FF	143	74.9	0.525	2.3	55
4. Ammonium sulfate fractionation	56.5	13.8	0.246	1.1	10.2
5. CM Sepharose FF	1.8	5.3	2.93	13	3.9
6. Bio-Scale CHT2-1	0.31	2.76	8.92	39.8	2
7. Superdex 200	0.00559	1.59	284	1,267	1.2

	Total protein	Total activity	Specific activity	Purification	Recovery
Step	(mg)	(nkat)	(nkat/mg protein)	(-fold)	(%)
1. Crude extract	607	136	0.224	1	100
2. DEAE Sephadex	208	116	0.558	2.5	84.9
3. SP Sepharose FF	143	74.9	0.525	2.3	55
4. Ammonium sulfate fractionation	56.5	13.8	0.246	1.1	10.2
5. CM Sepharose FF	1.8	5.3	2.93	13	3.9
6. Bio-Scale CHT2-1	0.31	2.76	8.92	39.8	2
7. Superdex 200	0.00559	1.59	284	1,267	1.2