Sequencing statistics . | Raw data . | Processed data . | ||
---|---|---|---|---|
Library . | Size (Gb) . | Coveragea . | Size (Gb) . | Coveragea . |
Paired-end 0.2-kb inserts | 28.9 | 15 | 26.1 | 14 |
Paired-end 0.5-kb inserts | 63.1 | 33 | 59.8 | 31 |
Mate-pair 2-kb inserts | 36.2 | 19 | 31.8 | 17 |
Mate-pair 5-Kb inserts | 34.3 | 18 | 27.8 | 14 |
Total | 162.5 | 85 | 145.5 | 76 |
Sequencing statistics . | Raw data . | Processed data . | ||
---|---|---|---|---|
Library . | Size (Gb) . | Coveragea . | Size (Gb) . | Coveragea . |
Paired-end 0.2-kb inserts | 28.9 | 15 | 26.1 | 14 |
Paired-end 0.5-kb inserts | 63.1 | 33 | 59.8 | 31 |
Mate-pair 2-kb inserts | 36.2 | 19 | 31.8 | 17 |
Mate-pair 5-Kb inserts | 34.3 | 18 | 27.8 | 14 |
Total | 162.5 | 85 | 145.5 | 76 |
Assembly statistics . | Contigs . | Scaffolds . | ||
---|---|---|---|---|
Total assembly size (Gb) | 1.6 | 1.6 | ||
Total assembled sequences | 219,073 | 148,874 | ||
Longest sequence length (kb) | 465 | 4,541 | ||
Average sequence length (kb) | 7.2 | 10.7 | ||
N90 indexb | 40,926 | 3,505 | ||
N90 length (kb) | 7.7 | 67.7 | ||
N50 index | 9,917 | 756 | ||
N50 length (kb) | 43.6 | 583 | ||
GC content (%) | 34.9 | 34.9 |
Assembly statistics . | Contigs . | Scaffolds . | ||
---|---|---|---|---|
Total assembly size (Gb) | 1.6 | 1.6 | ||
Total assembled sequences | 219,073 | 148,874 | ||
Longest sequence length (kb) | 465 | 4,541 | ||
Average sequence length (kb) | 7.2 | 10.7 | ||
N90 indexb | 40,926 | 3,505 | ||
N90 length (kb) | 7.7 | 67.7 | ||
N50 index | 9,917 | 756 | ||
N50 length (kb) | 43.6 | 583 | ||
GC content (%) | 34.9 | 34.9 |
Gene space statistics . | Mapping percentage . | |||||
---|---|---|---|---|---|---|
Laupala unigenes from the Gene Index | 95 | |||||
Laupala RNA-seq reads | 92 |
Gene space statistics . | Mapping percentage . | |||||
---|---|---|---|---|---|---|
Laupala unigenes from the Gene Index | 95 | |||||
Laupala RNA-seq reads | 92 |
BUSCO database . | Complete (%) . | Single copy (%) . | Duplicated (%) . | Fragmented (%) . | Missing (%) . | Total . |
---|---|---|---|---|---|---|
Eukaryota_odb9 | 98.7 | 93.7 | 5.0 | 0.3 | 1.0 | 303 |
Arthropoda_odb9 | 99.3 | 96.8 | 2.5 | 0.1 | 0.6 | 1066 |
BUSCO database . | Complete (%) . | Single copy (%) . | Duplicated (%) . | Fragmented (%) . | Missing (%) . | Total . |
---|---|---|---|---|---|---|
Eukaryota_odb9 | 98.7 | 93.7 | 5.0 | 0.3 | 1.0 | 303 |
Arthropoda_odb9 | 99.3 | 96.8 | 2.5 | 0.1 | 0.6 | 1066 |
Coverage is based on an estimated genome size of 1.91 Gb (Petrov et al. 2000).
When ordering all contigs (or scaffolds) by size, the N50 or N90 index indicates the number of the longest sequences (contigs or scaffolds) that contain 50 or 90% of the total assembled sequence, respectively. The N50 and N90 length indicate the length of the shortest sequence in the set of the largest contigs (or scaffolds) that contain 50 or 90% of all the sequence in the assembly, respectively.
Sequencing statistics . | Raw data . | Processed data . | ||
---|---|---|---|---|
Library . | Size (Gb) . | Coveragea . | Size (Gb) . | Coveragea . |
Paired-end 0.2-kb inserts | 28.9 | 15 | 26.1 | 14 |
Paired-end 0.5-kb inserts | 63.1 | 33 | 59.8 | 31 |
Mate-pair 2-kb inserts | 36.2 | 19 | 31.8 | 17 |
Mate-pair 5-Kb inserts | 34.3 | 18 | 27.8 | 14 |
Total | 162.5 | 85 | 145.5 | 76 |
Sequencing statistics . | Raw data . | Processed data . | ||
---|---|---|---|---|
Library . | Size (Gb) . | Coveragea . | Size (Gb) . | Coveragea . |
Paired-end 0.2-kb inserts | 28.9 | 15 | 26.1 | 14 |
Paired-end 0.5-kb inserts | 63.1 | 33 | 59.8 | 31 |
Mate-pair 2-kb inserts | 36.2 | 19 | 31.8 | 17 |
Mate-pair 5-Kb inserts | 34.3 | 18 | 27.8 | 14 |
Total | 162.5 | 85 | 145.5 | 76 |
Assembly statistics . | Contigs . | Scaffolds . | ||
---|---|---|---|---|
Total assembly size (Gb) | 1.6 | 1.6 | ||
Total assembled sequences | 219,073 | 148,874 | ||
Longest sequence length (kb) | 465 | 4,541 | ||
Average sequence length (kb) | 7.2 | 10.7 | ||
N90 indexb | 40,926 | 3,505 | ||
N90 length (kb) | 7.7 | 67.7 | ||
N50 index | 9,917 | 756 | ||
N50 length (kb) | 43.6 | 583 | ||
GC content (%) | 34.9 | 34.9 |
Assembly statistics . | Contigs . | Scaffolds . | ||
---|---|---|---|---|
Total assembly size (Gb) | 1.6 | 1.6 | ||
Total assembled sequences | 219,073 | 148,874 | ||
Longest sequence length (kb) | 465 | 4,541 | ||
Average sequence length (kb) | 7.2 | 10.7 | ||
N90 indexb | 40,926 | 3,505 | ||
N90 length (kb) | 7.7 | 67.7 | ||
N50 index | 9,917 | 756 | ||
N50 length (kb) | 43.6 | 583 | ||
GC content (%) | 34.9 | 34.9 |
Gene space statistics . | Mapping percentage . | |||||
---|---|---|---|---|---|---|
Laupala unigenes from the Gene Index | 95 | |||||
Laupala RNA-seq reads | 92 |
Gene space statistics . | Mapping percentage . | |||||
---|---|---|---|---|---|---|
Laupala unigenes from the Gene Index | 95 | |||||
Laupala RNA-seq reads | 92 |
BUSCO database . | Complete (%) . | Single copy (%) . | Duplicated (%) . | Fragmented (%) . | Missing (%) . | Total . |
---|---|---|---|---|---|---|
Eukaryota_odb9 | 98.7 | 93.7 | 5.0 | 0.3 | 1.0 | 303 |
Arthropoda_odb9 | 99.3 | 96.8 | 2.5 | 0.1 | 0.6 | 1066 |
BUSCO database . | Complete (%) . | Single copy (%) . | Duplicated (%) . | Fragmented (%) . | Missing (%) . | Total . |
---|---|---|---|---|---|---|
Eukaryota_odb9 | 98.7 | 93.7 | 5.0 | 0.3 | 1.0 | 303 |
Arthropoda_odb9 | 99.3 | 96.8 | 2.5 | 0.1 | 0.6 | 1066 |
Coverage is based on an estimated genome size of 1.91 Gb (Petrov et al. 2000).
When ordering all contigs (or scaffolds) by size, the N50 or N90 index indicates the number of the longest sequences (contigs or scaffolds) that contain 50 or 90% of the total assembled sequence, respectively. The N50 and N90 length indicate the length of the shortest sequence in the set of the largest contigs (or scaffolds) that contain 50 or 90% of all the sequence in the assembly, respectively.
This PDF is available to Subscribers Only
View Article Abstract & Purchase OptionsFor full access to this pdf, sign in to an existing account, or purchase an annual subscription.