| Gene name . | Target site sequence . | GC content . | Editing efficiency . | secondary structure of sgRNA . |
|---|---|---|---|---|
SlEIN2 target | AGCCCGATTTGGGTTTGATTTGG regular site | 45% | 3/11 | Fig. 5d |
SlERFE1 target | AAACGAGCTCGACCCTCTACAGG regular site | 55% | 3/7 | Fig. 5e |
SlARF2B target | CTTGTGACAGTGCCATGTGAAGG irregular target | 50% | 2/5 | Fig. 5f |
| SlGRAS8 target 1 | TGAAGTAAAAGGCAGCTCGTTGG irregular target | 45% | 182/182 | Fig. 5a |
| SlGRAS8 target 2 | ACTGAGTCAGCGATTCGCTGTGG regular site | 55% | 182/182 | Fig. 5b |
| SlGRAS8 target 3 | CAAATTAGAACCGTTGGTAGCGG irregular target | 40% | 126/182 | Fig. 5c |
| SlACS2&4 target 1 | GCTTTCCACCCATCAAAATATGG irregular target | 40% | 0/9 | Fig. 5g |
| SlACS2&4 target 2 | GCCAACTTTCAAGATTATCATGG irregular target | 35% | 6/9 | Fig. 5h |
| Gene name . | Target site sequence . | GC content . | Editing efficiency . | secondary structure of sgRNA . |
|---|---|---|---|---|
SlEIN2 target | AGCCCGATTTGGGTTTGATTTGG regular site | 45% | 3/11 | Fig. 5d |
SlERFE1 target | AAACGAGCTCGACCCTCTACAGG regular site | 55% | 3/7 | Fig. 5e |
SlARF2B target | CTTGTGACAGTGCCATGTGAAGG irregular target | 50% | 2/5 | Fig. 5f |
| SlGRAS8 target 1 | TGAAGTAAAAGGCAGCTCGTTGG irregular target | 45% | 182/182 | Fig. 5a |
| SlGRAS8 target 2 | ACTGAGTCAGCGATTCGCTGTGG regular site | 55% | 182/182 | Fig. 5b |
| SlGRAS8 target 3 | CAAATTAGAACCGTTGGTAGCGG irregular target | 40% | 126/182 | Fig. 5c |
| SlACS2&4 target 1 | GCTTTCCACCCATCAAAATATGG irregular target | 40% | 0/9 | Fig. 5g |
| SlACS2&4 target 2 | GCCAACTTTCAAGATTATCATGG irregular target | 35% | 6/9 | Fig. 5h |
| Gene name . | Target site sequence . | GC content . | Editing efficiency . | secondary structure of sgRNA . |
|---|---|---|---|---|
SlEIN2 target | AGCCCGATTTGGGTTTGATTTGG regular site | 45% | 3/11 | Fig. 5d |
SlERFE1 target | AAACGAGCTCGACCCTCTACAGG regular site | 55% | 3/7 | Fig. 5e |
SlARF2B target | CTTGTGACAGTGCCATGTGAAGG irregular target | 50% | 2/5 | Fig. 5f |
| SlGRAS8 target 1 | TGAAGTAAAAGGCAGCTCGTTGG irregular target | 45% | 182/182 | Fig. 5a |
| SlGRAS8 target 2 | ACTGAGTCAGCGATTCGCTGTGG regular site | 55% | 182/182 | Fig. 5b |
| SlGRAS8 target 3 | CAAATTAGAACCGTTGGTAGCGG irregular target | 40% | 126/182 | Fig. 5c |
| SlACS2&4 target 1 | GCTTTCCACCCATCAAAATATGG irregular target | 40% | 0/9 | Fig. 5g |
| SlACS2&4 target 2 | GCCAACTTTCAAGATTATCATGG irregular target | 35% | 6/9 | Fig. 5h |
| Gene name . | Target site sequence . | GC content . | Editing efficiency . | secondary structure of sgRNA . |
|---|---|---|---|---|
SlEIN2 target | AGCCCGATTTGGGTTTGATTTGG regular site | 45% | 3/11 | Fig. 5d |
SlERFE1 target | AAACGAGCTCGACCCTCTACAGG regular site | 55% | 3/7 | Fig. 5e |
SlARF2B target | CTTGTGACAGTGCCATGTGAAGG irregular target | 50% | 2/5 | Fig. 5f |
| SlGRAS8 target 1 | TGAAGTAAAAGGCAGCTCGTTGG irregular target | 45% | 182/182 | Fig. 5a |
| SlGRAS8 target 2 | ACTGAGTCAGCGATTCGCTGTGG regular site | 55% | 182/182 | Fig. 5b |
| SlGRAS8 target 3 | CAAATTAGAACCGTTGGTAGCGG irregular target | 40% | 126/182 | Fig. 5c |
| SlACS2&4 target 1 | GCTTTCCACCCATCAAAATATGG irregular target | 40% | 0/9 | Fig. 5g |
| SlACS2&4 target 2 | GCCAACTTTCAAGATTATCATGG irregular target | 35% | 6/9 | Fig. 5h |
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