Table 1
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Summary of targeted deletions with the CRISPR/Cas9 system

# sgRNAsSequence 5′–3′PAM 5′–3′GC content (%)Target lociDeletion mutants/# of Hyg+T1 plants
sgRNA1GGGGTAGGGTTCAATTGAAGAGG50AP1 exon 18/23
sgRNA2AGATACTTGAACGCTATGAGAGG40AP1 exon 2
sgRNA3GACATCGGCGTCGCAGAGAACGG60SVP exon 15/17
sgRNA4ACTGCAAGTTATGCCTCTCTAGG45SVP exon 2
sgRNA5CTTCTGTTTCCTCCAAGCCTAGG50TFL1 exon 110/30
sgRNA6ATGATAGACCCAGATGTTCCAGG45TFL1 exon 2
# sgRNAsSequence 5′–3′PAM 5′–3′GC content (%)Target lociDeletion mutants/# of Hyg+T1 plants
sgRNA1GGGGTAGGGTTCAATTGAAGAGG50AP1 exon 18/23
sgRNA2AGATACTTGAACGCTATGAGAGG40AP1 exon 2
sgRNA3GACATCGGCGTCGCAGAGAACGG60SVP exon 15/17
sgRNA4ACTGCAAGTTATGCCTCTCTAGG45SVP exon 2
sgRNA5CTTCTGTTTCCTCCAAGCCTAGG50TFL1 exon 110/30
sgRNA6ATGATAGACCCAGATGTTCCAGG45TFL1 exon 2
Table 1
Open in new tab

Summary of targeted deletions with the CRISPR/Cas9 system

# sgRNAsSequence 5′–3′PAM 5′–3′GC content (%)Target lociDeletion mutants/# of Hyg+T1 plants
sgRNA1GGGGTAGGGTTCAATTGAAGAGG50AP1 exon 18/23
sgRNA2AGATACTTGAACGCTATGAGAGG40AP1 exon 2
sgRNA3GACATCGGCGTCGCAGAGAACGG60SVP exon 15/17
sgRNA4ACTGCAAGTTATGCCTCTCTAGG45SVP exon 2
sgRNA5CTTCTGTTTCCTCCAAGCCTAGG50TFL1 exon 110/30
sgRNA6ATGATAGACCCAGATGTTCCAGG45TFL1 exon 2
# sgRNAsSequence 5′–3′PAM 5′–3′GC content (%)Target lociDeletion mutants/# of Hyg+T1 plants
sgRNA1GGGGTAGGGTTCAATTGAAGAGG50AP1 exon 18/23
sgRNA2AGATACTTGAACGCTATGAGAGG40AP1 exon 2
sgRNA3GACATCGGCGTCGCAGAGAACGG60SVP exon 15/17
sgRNA4ACTGCAAGTTATGCCTCTCTAGG45SVP exon 2
sgRNA5CTTCTGTTTCCTCCAAGCCTAGG50TFL1 exon 110/30
sgRNA6ATGATAGACCCAGATGTTCCAGG45TFL1 exon 2
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