Table 2. Open in new tab Identification...

Patient

Category

Blood Culture Test

Taxonomic Hierarchy

NGS-Identified Pathogen

RPM^a (Reads), No.

Coverage, %

FN006

Negative

Family

Sphingomonadaceae

513

…

Caulobacteraceae

487

…

FN008

Rhizobium radiobacter

Genus

Agrobacterium

312

…

FN010

Negative

Family

Sphingomonadaceae

282

…

FN021

Acinetobacter ursingii

Species

Acinetobacter ursingii

247 671

85.5

FN022

Negative

Genus

Pseudomonas

1395

…

Ralstonia

977

…

FN025

Negative

Genus

Pseudomonas

290

…

FN034

Negative

Family

Sphingomonadaceae

500

…

Comamonadaceae

379

…

Pseudomonadaceae

283

…

FN037

Negative

Species

Acinetobacter soli

1170

3.6

FN061

Negative

Genus

Brevundimonas

1426

…

Acinetobacter

312

…

FN063

Negative

Family

Staphylococcaceae

261

…

FN070

Streptococcus mitis/oralis

Species

Acinetobacter soli

1266

14.7

Streptococcus mitis

687

28.1

Brevundimonas sp DS20

334

7.5

Malassezia restricta

228

3.4

FN071

Negative

Species

Burkholderia cepacia

345

1.0

FN073

Negative

Genus

Escherichia

319

…

Acinetobacter

237

…

FN088

Streptococcus mitis/oralis

Species

Acinetobacter soli

758

6.9

Brevundimonas sp DS20

268

5.6

FN093

Negative

Genus

Roseomonas

452

…

FN099

Streptococcus mitis/oralis

Genus

Methylobacterium

430

…

FN100

Negative

Species

Acinetobacter soli

306

35.3

FN101

Negative

Species

Klebsiella variicola

406

6.8

FN104

Negative

Genus

Acinetobacter

505

…

Brevundimonas

262

…

FN106

Negative

Family

Enterobacteriaceae

551

…

Staphylococcaceae

203

…

NE01

…

Genus

Acinetobacter

617

…

NE04

…

Genus

Acinetobacter

396

…

NE07

…

Genus

Pseudomonas

1590

…

Brevundimonas

205

…

Table 2.

Open in new tab

Identification of Putative Bacteria by Next-Generation Sequencing From Study Samples

Patient	Category	Blood Culture Test	Taxonomic Hierarchy	NGS-Identified Pathogen	RPM^a (Reads), No.	Coverage, %
FN006	FN	Negative	Family	Sphingomonadaceae	513	…
				Caulobacteraceae	487	…
FN008	FN	*Rhizobium radiobacter*	Genus	*Agrobacterium*	312	…
FN010	FN	Negative	Family	Sphingomonadaceae	282	…
FN021	FN	*Acinetobacter ursingii*	Species	*Acinetobacter ursingii*	247 671	85.5
FN022	FN	Negative	Genus	Pseudomonas	1395	…
				Ralstonia	977	…
FN025	FN	Negative	Genus	Pseudomonas	290	…
FN034	FN	Negative	Family	Sphingomonadaceae	500	…
				Comamonadaceae	379	…
				Pseudomonadaceae	283	…
FN037	FN	Negative	Species	Acinetobacter soli	1170	3.6
FN061	FN	Negative	Genus	Brevundimonas	1426	…
				Acinetobacter	312	…
FN063	FN	Negative	Family	Staphylococcaceae	261	…
FN070	FN	*Streptococcus mitis/oralis*	Species	Acinetobacter soli	1266	14.7
				*Streptococcus mitis*	687	28.1
				Brevundimonas sp DS20	334	7.5
				Malassezia restricta	228	3.4
FN071	FN	Negative	Species	Burkholderia cepacia	345	1.0
FN073	FN	Negative	Genus	Escherichia	319	…
				Acinetobacter	237	…
FN088	FN	*Streptococcus mitis/oralis*	Species	Acinetobacter soli	758	6.9
				Brevundimonas sp DS20	268	5.6
FN093	FN	Negative	Genus	Roseomonas	452	…
FN099	FN	*Streptococcus mitis/oralis*	Genus	Methylobacterium	430	…
FN100	FN	Negative	Species	Acinetobacter soli	306	35.3
FN101	FN	Negative	Species	Klebsiella variicola	406	6.8
FN104	FN	Negative	Genus	Acinetobacter	505	…
				Brevundimonas	262	…
FN106	FN	Negative	Family	Enterobacteriaceae	551	…
				Staphylococcaceae	203	…
NE01	NE	…	Genus	Acinetobacter	617	…
NE04	NE	…	Genus	Acinetobacter	396	…
NE07	NE	…	Genus	Pseudomonas	1590	…
				Brevundimonas	205	…

Patient	Category	Blood Culture Test	Taxonomic Hierarchy	NGS-Identified Pathogen	RPM^a (Reads), No.	Coverage, %
FN006	FN	Negative	Family	Sphingomonadaceae	513	…
				Caulobacteraceae	487	…
FN008	FN	*Rhizobium radiobacter*	Genus	*Agrobacterium*	312	…
FN010	FN	Negative	Family	Sphingomonadaceae	282	…
FN021	FN	*Acinetobacter ursingii*	Species	*Acinetobacter ursingii*	247 671	85.5
FN022	FN	Negative	Genus	Pseudomonas	1395	…
				Ralstonia	977	…
FN025	FN	Negative	Genus	Pseudomonas	290	…
FN034	FN	Negative	Family	Sphingomonadaceae	500	…
				Comamonadaceae	379	…
				Pseudomonadaceae	283	…
FN037	FN	Negative	Species	Acinetobacter soli	1170	3.6
FN061	FN	Negative	Genus	Brevundimonas	1426	…
				Acinetobacter	312	…
FN063	FN	Negative	Family	Staphylococcaceae	261	…
FN070	FN	*Streptococcus mitis/oralis*	Species	Acinetobacter soli	1266	14.7
				*Streptococcus mitis*	687	28.1
				Brevundimonas sp DS20	334	7.5
				Malassezia restricta	228	3.4
FN071	FN	Negative	Species	Burkholderia cepacia	345	1.0
FN073	FN	Negative	Genus	Escherichia	319	…
				Acinetobacter	237	…
FN088	FN	*Streptococcus mitis/oralis*	Species	Acinetobacter soli	758	6.9
				Brevundimonas sp DS20	268	5.6
FN093	FN	Negative	Genus	Roseomonas	452	…
FN099	FN	*Streptococcus mitis/oralis*	Genus	Methylobacterium	430	…
FN100	FN	Negative	Species	Acinetobacter soli	306	35.3
FN101	FN	Negative	Species	Klebsiella variicola	406	6.8
FN104	FN	Negative	Genus	Acinetobacter	505	…
				Brevundimonas	262	…
FN106	FN	Negative	Family	Enterobacteriaceae	551	…
				Staphylococcaceae	203	…
NE01	NE	…	Genus	Acinetobacter	617	…
NE04	NE	…	Genus	Acinetobacter	396	…
NE07	NE	…	Genus	Pseudomonas	1590	…
				Brevundimonas	205	…

Bacteria detected in blood cultures are shown in bold.

Abbreviations: FN, febrile neutropenia; NE, neutropenia without fever; NGS, next-generation sequencing.

^aRPM indicates the number of reads assigned each pathogen per million total sequencing reads.

This Feature Is Available To Subscribers Only