Table 3.

Sequence and Structural Analysis of Conserved asRNAs.

ML Distance of AA Sequences
Alignment LengthasRNA RegionbNon-sRNA Region of ProteinΔGc (BC/DC)dNo. of Compensatory Changes (BC/DC)No. of Aligned Carsonella SequencesΔG (ALL)eNo. of Compensatory Changes (ALL)No. of Shared Pairs% of Shared Pairs
aroC890.1970.208−29.4211−7.6100%
atpA_11110.1320.229−42.6511−22.841853%
atpA_21780.1170.238−63.4511−30.151936%
atpD2400.0740.107−90.2611−55.473948%
carA-carB3290.4870.458−126.899−51.3101413%
carB1070.4370.312−26.829−6.35515%
clpX1380.1700.220−42.2611−13.22511%
gidA1500.3730.231−45.0511−13.7400%
grpE1450.3610.569−44.1611−10.06511%
leuC1930.1230.265−65.4711−30.3700%
prfA1430.1220.435−37.3211−18.121639%
ML Distance of AA Sequences
Alignment LengthasRNA RegionbNon-sRNA Region of ProteinΔGc (BC/DC)dNo. of Compensatory Changes (BC/DC)No. of Aligned Carsonella SequencesΔG (ALL)eNo. of Compensatory Changes (ALL)No. of Shared Pairs% of Shared Pairs
aroC890.1970.208−29.4211−7.6100%
atpA_11110.1320.229−42.6511−22.841853%
atpA_21780.1170.238−63.4511−30.151936%
atpD2400.0740.107−90.2611−55.473948%
carA-carB3290.4870.458−126.899−51.3101413%
carB1070.4370.312−26.829−6.35515%
clpX1380.1700.220−42.2611−13.22511%
gidA1500.3730.231−45.0511−13.7400%
grpE1450.3610.569−44.1611−10.06511%
leuC1930.1230.265−65.4711−30.3700%
prfA1430.1220.435−37.3211−18.121639%
a

Length of overlapping sRNA region with additional 15 nt upstream and downstream in Carsonella-BC and Carsonella-DC.

b

Values in italics are significantly more conserved than surrounding protein coding region in one-tailed t-test, P <0.01.

c

Thermodynamic ensemble prediction (kcal/mol) from RNAalifold.

d

BC/DC = Carsonella-BC and Carsonella-DC.

e

ALL = all aligned Carsonella strains.

Table 3.

Sequence and Structural Analysis of Conserved asRNAs.

ML Distance of AA Sequences
Alignment LengthasRNA RegionbNon-sRNA Region of ProteinΔGc (BC/DC)dNo. of Compensatory Changes (BC/DC)No. of Aligned Carsonella SequencesΔG (ALL)eNo. of Compensatory Changes (ALL)No. of Shared Pairs% of Shared Pairs
aroC890.1970.208−29.4211−7.6100%
atpA_11110.1320.229−42.6511−22.841853%
atpA_21780.1170.238−63.4511−30.151936%
atpD2400.0740.107−90.2611−55.473948%
carA-carB3290.4870.458−126.899−51.3101413%
carB1070.4370.312−26.829−6.35515%
clpX1380.1700.220−42.2611−13.22511%
gidA1500.3730.231−45.0511−13.7400%
grpE1450.3610.569−44.1611−10.06511%
leuC1930.1230.265−65.4711−30.3700%
prfA1430.1220.435−37.3211−18.121639%
ML Distance of AA Sequences
Alignment LengthasRNA RegionbNon-sRNA Region of ProteinΔGc (BC/DC)dNo. of Compensatory Changes (BC/DC)No. of Aligned Carsonella SequencesΔG (ALL)eNo. of Compensatory Changes (ALL)No. of Shared Pairs% of Shared Pairs
aroC890.1970.208−29.4211−7.6100%
atpA_11110.1320.229−42.6511−22.841853%
atpA_21780.1170.238−63.4511−30.151936%
atpD2400.0740.107−90.2611−55.473948%
carA-carB3290.4870.458−126.899−51.3101413%
carB1070.4370.312−26.829−6.35515%
clpX1380.1700.220−42.2611−13.22511%
gidA1500.3730.231−45.0511−13.7400%
grpE1450.3610.569−44.1611−10.06511%
leuC1930.1230.265−65.4711−30.3700%
prfA1430.1220.435−37.3211−18.121639%
a

Length of overlapping sRNA region with additional 15 nt upstream and downstream in Carsonella-BC and Carsonella-DC.

b

Values in italics are significantly more conserved than surrounding protein coding region in one-tailed t-test, P <0.01.

c

Thermodynamic ensemble prediction (kcal/mol) from RNAalifold.

d

BC/DC = Carsonella-BC and Carsonella-DC.

e

ALL = all aligned Carsonella strains.

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