Table 1.

Detection of V. alginolyticus in clinical samples

No.Sample typeSpeciesTissueDetection results
Real-time RPA (Tt, min)qPCR (Ct, n = 3)
1ShrimpExopalamon carinc audahepatopancreas
2ShrimpExopalamon carincaudahepatopancreas
3ShrimpExopalamon carincaudahepatopancreas
4ShrimpExopalamon carincaudahepatopancreas925.27 ± 0.23
5ShrimpExopalamon carincaudagill
6ShrimpExopalamon carincaudagill
7ShrimpExopalamon carincaudagill
8ShrimpExopalamon carincaudagill
9ShrimpLitopenaeus vannameihepatopancreas
10ShrimpLitopenaeus vannameihepatopancreas
11ShrimpLitopenaeus vannameihepatopancreas
12ShrimpLitopenaeus vannameihepatopancreas
13ShrimpLitopenaeus vannameihepatopancreas
14ShrimpLitopenaeus vannameihepatopancreas7.2521.89 ± 0.15
15ShrimpLitopenaeus vannameihepatopancreas
16ShrimpLitopenaeus vannameihepatopancreas
17ShrimpLitopenaeus vannameihepatopancreas
18ShrimpLitopenaeus vannameihepatopancreas
19ShrimpLitopenaeus vannameihepatopancreas
20ShrimpLitopenaeus vannameihepatopancreas
21ShrimpLitopenaeus vannameihepatopancreas513.01 ± 0.22
22ShrimpLitopenaeus vannameihepatopancreas
23ShrimpLitopenaeus vannameihepatopancreas
24ShrimpLitopenaeus vannameihepatopancreas48.99 ± 0.13
25ShrimpLitopenaeus vannameihepatopancreas3.7512.43 ± 0.12
26ShrimpLitopenaeus vannameihepatopancreas
27ShrimpLitopenaeus vannameihepatopancreas
28ShrimpLitopenaeus vannameihepatopancreas
29ShrimpLitopenaeus vannameihepatopancreas
30ShrimpLitopenaeus vannameihepatopancreas
31ShrimpLitopenaeus vannameihepatopancreas
32ShrimpLitopenaeus vannameihepatopancreas
33ShrimpLitopenaeus vannameihepatopancreas
34ShrimpLitopenaeus vannameihepatopancreas
35ShrimpLitopenaeus vannameihepatopancreas
36ShrimpLitopenaeus vannameihepatopancreas
37ShrimpLitopenaeus vannameihepatopancreas
38ShrimpLitopenaeus vannameihepatopancreas
39ShrimpLitopenaeus vannameihepatopancreas3.510.08 ± 0.24
40ShrimpLitopenaeus vannameihepatopancreas
41ShrimpLitopenaeus vannameihepatopancreas
42ShrimpLitopenaeus vannameihepatopancreas
43ShrimpLitopenaeus vannameihepatopancreas
44ShrimpLitopenaeus vannameihepatopancreas
45ShrimpLitopenaeus vannameihepatopancreas
46ShrimpLitopenaeus vannameihepatopancreas
47ShrimpLitopenaeus vannameiintestine
48ShrimpLitopenaeus vannameiintestine
49ShrimpLitopenaeus vannameiintestine
50ShrimpLitopenaeus vannameiintestine
51ShrimpLitopenaeus vannameigill
52ShrimpLitopenaeus vannameigill
53ShrimpLitopenaeus vannameigill
54ShrimpLitopenaeus vannameigill
55ShrimpLitopenaeus vannameigill
56Shellfishostrea gigas thunbergmeat
57Shellfishostrea gigas thunbergmeat
58Shellfishostrea gigas thunbergmeat
59Shellfishostrea gigas thunbergmeat5.7520.11 ± 0.15
60Shellfishostrea gigas thunbergmeat
61Shellfishostrea gigas thunbergmeat
62Shellfishostrea gigas thunbergmeat
63Shellfishostrea gigas thunbergmeat
64Shellfishostrea gigas thunbergmeat
65Shellfishostrea gigas thunbergmeat
66Shellfishostrea gigas thunbergmeat
67Shellfishostrea gigas thunbergmeat411.51 ± 0.21
68Shellfishostrea gigas thunbergmeat5.8616.92 ± 0.31
69Shellfishostrea gigas thunbergmeat
70Shellfishostrea gigas thunbergmeat
71Shellfishostrea gigas thunbergmeat
72Shellfishostrea gigas thunbergmeat9.2525.85 ± 0.18
73Shellfishostrea gigas thunbergmeat
74Shellfishostrea gigas thunbergmeat
75Shellfishostrea gigas thunbergmeat
76Shellfishostrea gigas thunbergmeat
77Shellfishostrea gigas thunbergmeat
78Shellfishostrea gigas thunbergmeat
79ShellfishMeretrix meretrixmeat10.826.13 ± 0.27
80ShellfishMeretrix meretrixmeat
No.Sample typeSpeciesTissueDetection results
Real-time RPA (Tt, min)qPCR (Ct, n = 3)
1ShrimpExopalamon carinc audahepatopancreas
2ShrimpExopalamon carincaudahepatopancreas
3ShrimpExopalamon carincaudahepatopancreas
4ShrimpExopalamon carincaudahepatopancreas925.27 ± 0.23
5ShrimpExopalamon carincaudagill
6ShrimpExopalamon carincaudagill
7ShrimpExopalamon carincaudagill
8ShrimpExopalamon carincaudagill
9ShrimpLitopenaeus vannameihepatopancreas
10ShrimpLitopenaeus vannameihepatopancreas
11ShrimpLitopenaeus vannameihepatopancreas
12ShrimpLitopenaeus vannameihepatopancreas
13ShrimpLitopenaeus vannameihepatopancreas
14ShrimpLitopenaeus vannameihepatopancreas7.2521.89 ± 0.15
15ShrimpLitopenaeus vannameihepatopancreas
16ShrimpLitopenaeus vannameihepatopancreas
17ShrimpLitopenaeus vannameihepatopancreas
18ShrimpLitopenaeus vannameihepatopancreas
19ShrimpLitopenaeus vannameihepatopancreas
20ShrimpLitopenaeus vannameihepatopancreas
21ShrimpLitopenaeus vannameihepatopancreas513.01 ± 0.22
22ShrimpLitopenaeus vannameihepatopancreas
23ShrimpLitopenaeus vannameihepatopancreas
24ShrimpLitopenaeus vannameihepatopancreas48.99 ± 0.13
25ShrimpLitopenaeus vannameihepatopancreas3.7512.43 ± 0.12
26ShrimpLitopenaeus vannameihepatopancreas
27ShrimpLitopenaeus vannameihepatopancreas
28ShrimpLitopenaeus vannameihepatopancreas
29ShrimpLitopenaeus vannameihepatopancreas
30ShrimpLitopenaeus vannameihepatopancreas
31ShrimpLitopenaeus vannameihepatopancreas
32ShrimpLitopenaeus vannameihepatopancreas
33ShrimpLitopenaeus vannameihepatopancreas
34ShrimpLitopenaeus vannameihepatopancreas
35ShrimpLitopenaeus vannameihepatopancreas
36ShrimpLitopenaeus vannameihepatopancreas
37ShrimpLitopenaeus vannameihepatopancreas
38ShrimpLitopenaeus vannameihepatopancreas
39ShrimpLitopenaeus vannameihepatopancreas3.510.08 ± 0.24
40ShrimpLitopenaeus vannameihepatopancreas
41ShrimpLitopenaeus vannameihepatopancreas
42ShrimpLitopenaeus vannameihepatopancreas
43ShrimpLitopenaeus vannameihepatopancreas
44ShrimpLitopenaeus vannameihepatopancreas
45ShrimpLitopenaeus vannameihepatopancreas
46ShrimpLitopenaeus vannameihepatopancreas
47ShrimpLitopenaeus vannameiintestine
48ShrimpLitopenaeus vannameiintestine
49ShrimpLitopenaeus vannameiintestine
50ShrimpLitopenaeus vannameiintestine
51ShrimpLitopenaeus vannameigill
52ShrimpLitopenaeus vannameigill
53ShrimpLitopenaeus vannameigill
54ShrimpLitopenaeus vannameigill
55ShrimpLitopenaeus vannameigill
56Shellfishostrea gigas thunbergmeat
57Shellfishostrea gigas thunbergmeat
58Shellfishostrea gigas thunbergmeat
59Shellfishostrea gigas thunbergmeat5.7520.11 ± 0.15
60Shellfishostrea gigas thunbergmeat
61Shellfishostrea gigas thunbergmeat
62Shellfishostrea gigas thunbergmeat
63Shellfishostrea gigas thunbergmeat
64Shellfishostrea gigas thunbergmeat
65Shellfishostrea gigas thunbergmeat
66Shellfishostrea gigas thunbergmeat
67Shellfishostrea gigas thunbergmeat411.51 ± 0.21
68Shellfishostrea gigas thunbergmeat5.8616.92 ± 0.31
69Shellfishostrea gigas thunbergmeat
70Shellfishostrea gigas thunbergmeat
71Shellfishostrea gigas thunbergmeat
72Shellfishostrea gigas thunbergmeat9.2525.85 ± 0.18
73Shellfishostrea gigas thunbergmeat
74Shellfishostrea gigas thunbergmeat
75Shellfishostrea gigas thunbergmeat
76Shellfishostrea gigas thunbergmeat
77Shellfishostrea gigas thunbergmeat
78Shellfishostrea gigas thunbergmeat
79ShellfishMeretrix meretrixmeat10.826.13 ± 0.27
80ShellfishMeretrix meretrixmeat
Table 1.

Detection of V. alginolyticus in clinical samples

No.Sample typeSpeciesTissueDetection results
Real-time RPA (Tt, min)qPCR (Ct, n = 3)
1ShrimpExopalamon carinc audahepatopancreas
2ShrimpExopalamon carincaudahepatopancreas
3ShrimpExopalamon carincaudahepatopancreas
4ShrimpExopalamon carincaudahepatopancreas925.27 ± 0.23
5ShrimpExopalamon carincaudagill
6ShrimpExopalamon carincaudagill
7ShrimpExopalamon carincaudagill
8ShrimpExopalamon carincaudagill
9ShrimpLitopenaeus vannameihepatopancreas
10ShrimpLitopenaeus vannameihepatopancreas
11ShrimpLitopenaeus vannameihepatopancreas
12ShrimpLitopenaeus vannameihepatopancreas
13ShrimpLitopenaeus vannameihepatopancreas
14ShrimpLitopenaeus vannameihepatopancreas7.2521.89 ± 0.15
15ShrimpLitopenaeus vannameihepatopancreas
16ShrimpLitopenaeus vannameihepatopancreas
17ShrimpLitopenaeus vannameihepatopancreas
18ShrimpLitopenaeus vannameihepatopancreas
19ShrimpLitopenaeus vannameihepatopancreas
20ShrimpLitopenaeus vannameihepatopancreas
21ShrimpLitopenaeus vannameihepatopancreas513.01 ± 0.22
22ShrimpLitopenaeus vannameihepatopancreas
23ShrimpLitopenaeus vannameihepatopancreas
24ShrimpLitopenaeus vannameihepatopancreas48.99 ± 0.13
25ShrimpLitopenaeus vannameihepatopancreas3.7512.43 ± 0.12
26ShrimpLitopenaeus vannameihepatopancreas
27ShrimpLitopenaeus vannameihepatopancreas
28ShrimpLitopenaeus vannameihepatopancreas
29ShrimpLitopenaeus vannameihepatopancreas
30ShrimpLitopenaeus vannameihepatopancreas
31ShrimpLitopenaeus vannameihepatopancreas
32ShrimpLitopenaeus vannameihepatopancreas
33ShrimpLitopenaeus vannameihepatopancreas
34ShrimpLitopenaeus vannameihepatopancreas
35ShrimpLitopenaeus vannameihepatopancreas
36ShrimpLitopenaeus vannameihepatopancreas
37ShrimpLitopenaeus vannameihepatopancreas
38ShrimpLitopenaeus vannameihepatopancreas
39ShrimpLitopenaeus vannameihepatopancreas3.510.08 ± 0.24
40ShrimpLitopenaeus vannameihepatopancreas
41ShrimpLitopenaeus vannameihepatopancreas
42ShrimpLitopenaeus vannameihepatopancreas
43ShrimpLitopenaeus vannameihepatopancreas
44ShrimpLitopenaeus vannameihepatopancreas
45ShrimpLitopenaeus vannameihepatopancreas
46ShrimpLitopenaeus vannameihepatopancreas
47ShrimpLitopenaeus vannameiintestine
48ShrimpLitopenaeus vannameiintestine
49ShrimpLitopenaeus vannameiintestine
50ShrimpLitopenaeus vannameiintestine
51ShrimpLitopenaeus vannameigill
52ShrimpLitopenaeus vannameigill
53ShrimpLitopenaeus vannameigill
54ShrimpLitopenaeus vannameigill
55ShrimpLitopenaeus vannameigill
56Shellfishostrea gigas thunbergmeat
57Shellfishostrea gigas thunbergmeat
58Shellfishostrea gigas thunbergmeat
59Shellfishostrea gigas thunbergmeat5.7520.11 ± 0.15
60Shellfishostrea gigas thunbergmeat
61Shellfishostrea gigas thunbergmeat
62Shellfishostrea gigas thunbergmeat
63Shellfishostrea gigas thunbergmeat
64Shellfishostrea gigas thunbergmeat
65Shellfishostrea gigas thunbergmeat
66Shellfishostrea gigas thunbergmeat
67Shellfishostrea gigas thunbergmeat411.51 ± 0.21
68Shellfishostrea gigas thunbergmeat5.8616.92 ± 0.31
69Shellfishostrea gigas thunbergmeat
70Shellfishostrea gigas thunbergmeat
71Shellfishostrea gigas thunbergmeat
72Shellfishostrea gigas thunbergmeat9.2525.85 ± 0.18
73Shellfishostrea gigas thunbergmeat
74Shellfishostrea gigas thunbergmeat
75Shellfishostrea gigas thunbergmeat
76Shellfishostrea gigas thunbergmeat
77Shellfishostrea gigas thunbergmeat
78Shellfishostrea gigas thunbergmeat
79ShellfishMeretrix meretrixmeat10.826.13 ± 0.27
80ShellfishMeretrix meretrixmeat
No.Sample typeSpeciesTissueDetection results
Real-time RPA (Tt, min)qPCR (Ct, n = 3)
1ShrimpExopalamon carinc audahepatopancreas
2ShrimpExopalamon carincaudahepatopancreas
3ShrimpExopalamon carincaudahepatopancreas
4ShrimpExopalamon carincaudahepatopancreas925.27 ± 0.23
5ShrimpExopalamon carincaudagill
6ShrimpExopalamon carincaudagill
7ShrimpExopalamon carincaudagill
8ShrimpExopalamon carincaudagill
9ShrimpLitopenaeus vannameihepatopancreas
10ShrimpLitopenaeus vannameihepatopancreas
11ShrimpLitopenaeus vannameihepatopancreas
12ShrimpLitopenaeus vannameihepatopancreas
13ShrimpLitopenaeus vannameihepatopancreas
14ShrimpLitopenaeus vannameihepatopancreas7.2521.89 ± 0.15
15ShrimpLitopenaeus vannameihepatopancreas
16ShrimpLitopenaeus vannameihepatopancreas
17ShrimpLitopenaeus vannameihepatopancreas
18ShrimpLitopenaeus vannameihepatopancreas
19ShrimpLitopenaeus vannameihepatopancreas
20ShrimpLitopenaeus vannameihepatopancreas
21ShrimpLitopenaeus vannameihepatopancreas513.01 ± 0.22
22ShrimpLitopenaeus vannameihepatopancreas
23ShrimpLitopenaeus vannameihepatopancreas
24ShrimpLitopenaeus vannameihepatopancreas48.99 ± 0.13
25ShrimpLitopenaeus vannameihepatopancreas3.7512.43 ± 0.12
26ShrimpLitopenaeus vannameihepatopancreas
27ShrimpLitopenaeus vannameihepatopancreas
28ShrimpLitopenaeus vannameihepatopancreas
29ShrimpLitopenaeus vannameihepatopancreas
30ShrimpLitopenaeus vannameihepatopancreas
31ShrimpLitopenaeus vannameihepatopancreas
32ShrimpLitopenaeus vannameihepatopancreas
33ShrimpLitopenaeus vannameihepatopancreas
34ShrimpLitopenaeus vannameihepatopancreas
35ShrimpLitopenaeus vannameihepatopancreas
36ShrimpLitopenaeus vannameihepatopancreas
37ShrimpLitopenaeus vannameihepatopancreas
38ShrimpLitopenaeus vannameihepatopancreas
39ShrimpLitopenaeus vannameihepatopancreas3.510.08 ± 0.24
40ShrimpLitopenaeus vannameihepatopancreas
41ShrimpLitopenaeus vannameihepatopancreas
42ShrimpLitopenaeus vannameihepatopancreas
43ShrimpLitopenaeus vannameihepatopancreas
44ShrimpLitopenaeus vannameihepatopancreas
45ShrimpLitopenaeus vannameihepatopancreas
46ShrimpLitopenaeus vannameihepatopancreas
47ShrimpLitopenaeus vannameiintestine
48ShrimpLitopenaeus vannameiintestine
49ShrimpLitopenaeus vannameiintestine
50ShrimpLitopenaeus vannameiintestine
51ShrimpLitopenaeus vannameigill
52ShrimpLitopenaeus vannameigill
53ShrimpLitopenaeus vannameigill
54ShrimpLitopenaeus vannameigill
55ShrimpLitopenaeus vannameigill
56Shellfishostrea gigas thunbergmeat
57Shellfishostrea gigas thunbergmeat
58Shellfishostrea gigas thunbergmeat
59Shellfishostrea gigas thunbergmeat5.7520.11 ± 0.15
60Shellfishostrea gigas thunbergmeat
61Shellfishostrea gigas thunbergmeat
62Shellfishostrea gigas thunbergmeat
63Shellfishostrea gigas thunbergmeat
64Shellfishostrea gigas thunbergmeat
65Shellfishostrea gigas thunbergmeat
66Shellfishostrea gigas thunbergmeat
67Shellfishostrea gigas thunbergmeat411.51 ± 0.21
68Shellfishostrea gigas thunbergmeat5.8616.92 ± 0.31
69Shellfishostrea gigas thunbergmeat
70Shellfishostrea gigas thunbergmeat
71Shellfishostrea gigas thunbergmeat
72Shellfishostrea gigas thunbergmeat9.2525.85 ± 0.18
73Shellfishostrea gigas thunbergmeat
74Shellfishostrea gigas thunbergmeat
75Shellfishostrea gigas thunbergmeat
76Shellfishostrea gigas thunbergmeat
77Shellfishostrea gigas thunbergmeat
78Shellfishostrea gigas thunbergmeat
79ShellfishMeretrix meretrixmeat10.826.13 ± 0.27
80ShellfishMeretrix meretrixmeat
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