Table 4. Open in new tab Duplex... | Oxford Academic

Table 4.

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Duplex real-time PCR detection stability results of pMON87712

Concentration (copies/μl)	Target sequence	Ct						SD	RSD%
		1	2	3	4	5	6	SD	RSD%
10⁵	Lectin	22.41	22.54	22.50	22.59	22.42	22.42	0.08	0.34
	MON87712	21.33	21.40	21.35	21.29	21.00	20.96	0.19	0.91
10⁴	Lectin	25.82	25.80	25.74	25.83	25.74	25.78	0.04	0.16
	MON87712	24.48	24.64	24.62	24.74	24.32	24.38	0.16	0.66
10³	Lectin	29.26	29.32	29.13	29.23	29.21	29.14	0.07	0.25
	MON87712	28.17	28.35	28.05	28.14	28.15	27.62	0.24	0.87
100	Lectin	32.50	32.56	32.24	32.59	32.64	32.43	0.14	0.44
	MON87712	31.23	31.69	31.44	31.52	31.65	31.29	0.19	0.60
50	Lectin	33.60	33.26	33.59	33.56	33.53	33.54	0.13	0.39
	MON87712	32.63	32.89	32.70	32.56	32.70	32.52	0.13	0.40
25	Lectin	34.18	34.08	34.23	34.46	34.34	34.29	0.13	0.39
	MON87712	35.68	33.33	33.67	33.92	33.75	33.20	0.90	2.65
10	Lectin	35.57	36.10	35.32	35.89	35.64	35.98	0.29	0.82
	MON87712	34.81	36.69	36.44	35.05	36.42	36.59	0.84	2.33

Concentration (copies/μl)	Target sequence	Ct						SD	RSD%
		1	2	3	4	5	6	SD	RSD%
10⁵	Lectin	22.41	22.54	22.50	22.59	22.42	22.42	0.08	0.34
	MON87712	21.33	21.40	21.35	21.29	21.00	20.96	0.19	0.91
10⁴	Lectin	25.82	25.80	25.74	25.83	25.74	25.78	0.04	0.16
	MON87712	24.48	24.64	24.62	24.74	24.32	24.38	0.16	0.66
10³	Lectin	29.26	29.32	29.13	29.23	29.21	29.14	0.07	0.25
	MON87712	28.17	28.35	28.05	28.14	28.15	27.62	0.24	0.87
100	Lectin	32.50	32.56	32.24	32.59	32.64	32.43	0.14	0.44
	MON87712	31.23	31.69	31.44	31.52	31.65	31.29	0.19	0.60
50	Lectin	33.60	33.26	33.59	33.56	33.53	33.54	0.13	0.39
	MON87712	32.63	32.89	32.70	32.56	32.70	32.52	0.13	0.40
25	Lectin	34.18	34.08	34.23	34.46	34.34	34.29	0.13	0.39
	MON87712	35.68	33.33	33.67	33.92	33.75	33.20	0.90	2.65
10	Lectin	35.57	36.10	35.32	35.89	35.64	35.98	0.29	0.82
	MON87712	34.81	36.69	36.44	35.05	36.42	36.59	0.84	2.33

SD, standard deviation.

Table 4.

Open in new tab

Duplex real-time PCR detection stability results of pMON87712

Concentration (copies/μl)	Target sequence	Ct						SD	RSD%
		1	2	3	4	5	6	SD	RSD%
10⁵	Lectin	22.41	22.54	22.50	22.59	22.42	22.42	0.08	0.34
	MON87712	21.33	21.40	21.35	21.29	21.00	20.96	0.19	0.91
10⁴	Lectin	25.82	25.80	25.74	25.83	25.74	25.78	0.04	0.16
	MON87712	24.48	24.64	24.62	24.74	24.32	24.38	0.16	0.66
10³	Lectin	29.26	29.32	29.13	29.23	29.21	29.14	0.07	0.25
	MON87712	28.17	28.35	28.05	28.14	28.15	27.62	0.24	0.87
100	Lectin	32.50	32.56	32.24	32.59	32.64	32.43	0.14	0.44
	MON87712	31.23	31.69	31.44	31.52	31.65	31.29	0.19	0.60
50	Lectin	33.60	33.26	33.59	33.56	33.53	33.54	0.13	0.39
	MON87712	32.63	32.89	32.70	32.56	32.70	32.52	0.13	0.40
25	Lectin	34.18	34.08	34.23	34.46	34.34	34.29	0.13	0.39
	MON87712	35.68	33.33	33.67	33.92	33.75	33.20	0.90	2.65
10	Lectin	35.57	36.10	35.32	35.89	35.64	35.98	0.29	0.82
	MON87712	34.81	36.69	36.44	35.05	36.42	36.59	0.84	2.33

Concentration (copies/μl)	Target sequence	Ct						SD	RSD%
		1	2	3	4	5	6	SD	RSD%
10⁵	Lectin	22.41	22.54	22.50	22.59	22.42	22.42	0.08	0.34
	MON87712	21.33	21.40	21.35	21.29	21.00	20.96	0.19	0.91
10⁴	Lectin	25.82	25.80	25.74	25.83	25.74	25.78	0.04	0.16
	MON87712	24.48	24.64	24.62	24.74	24.32	24.38	0.16	0.66
10³	Lectin	29.26	29.32	29.13	29.23	29.21	29.14	0.07	0.25
	MON87712	28.17	28.35	28.05	28.14	28.15	27.62	0.24	0.87
100	Lectin	32.50	32.56	32.24	32.59	32.64	32.43	0.14	0.44
	MON87712	31.23	31.69	31.44	31.52	31.65	31.29	0.19	0.60
50	Lectin	33.60	33.26	33.59	33.56	33.53	33.54	0.13	0.39
	MON87712	32.63	32.89	32.70	32.56	32.70	32.52	0.13	0.40
25	Lectin	34.18	34.08	34.23	34.46	34.34	34.29	0.13	0.39
	MON87712	35.68	33.33	33.67	33.92	33.75	33.20	0.90	2.65
10	Lectin	35.57	36.10	35.32	35.89	35.64	35.98	0.29	0.82
	MON87712	34.81	36.69	36.44	35.05	36.42	36.59	0.84	2.33

SD, standard deviation.