Table 3.
Open in new tab

The specificity of the duplex real-time PCR

Serial numberEventSpeciesSourceSoybean endogenous lectin geneMON87712 event-specific gene
Expected presenceAmplification curve CtExpected presenceAmplification curve Ct
1MON87712SoybeanPlasmidYes+Yes+
2GTS-40-3-2SoybeanIRMMYes+No–
3DP356043SoybeanIRMMYes+No–
4DP305423SoybeanIRMMYes+No–
5DAS 68416-4SoybeanIRMMYes+No–
6A5547-127SoybeanAOCSYes+No–
7MON89788SoybeanAOCSYes+No–
8A2704-12SoybeanAOCSYes+No–
9MON87701SoybeanAOCSYes+No–
10CV127SoybeanAOCSYes+No–
11MON87705SoybeanAOCSYes+No–
12MON87769SoybeanAOCSYes+No–
13FG72SoybeanAOCSYes+No–
14MON87708SoybeanAOCSYes+No–
15DAS-81419-2SoybeanIRMMYes+No–
16DAS-44406-6SoybeanIRMMYes+No–
17MON87751SoybeanAOCSYes+No–
18Non-GM soybeanSoybeanAOCSYes+No–
19Non-GM soybeanSoybeanAOCSYes+No–
20GA21CornAOCSNo–No–
21TC1507CornIRMMNo–No–
22MON87460CornAOCSNo–No–
23MIR162CornAOCSNo–No–
24MIR604CornAOCSNo–No–
25MON88302RapeAOCSNo–No–
26GT73RapeAOCSNo–No–
27Rf2RapeAOCSNo–No–
28Ms8RapeAOCSNo–No–
29Ms1RapeAOCSNo–No–
3073496RapeIRMMNo–No–
31Rf1RapeAOCSNo–No–
32Topas 19/2RapeAOCSNo–No–
33Rf3RapeAOCSNo–No–
34T45RapeAOCSNo–No–
35AM04-1020PotatoIRMMNo–No–
36EH92-527-1PotatoIRMMNo–No–
37Corn negative sampleCornLab sampleNo–No–
38AlfalfaAlfalfaLab sampleNo–No–
39Black soybeanBlack soybeanLab sampleNo–No–
40Red beanRed beanLab sampleNo–No–
41Mung beanMung beanLab sampleNo–No–
42PeanutPeanutLab sampleNo–No–
43RiceRiceLab sampleNo–No–
44Genetically modified cottonseedCottonseedLab sampleNo–No–
45BarleyBarleyLab sampleNo–No–
46WheatWheatLab sampleNo–No–
Serial numberEventSpeciesSourceSoybean endogenous lectin geneMON87712 event-specific gene
Expected presenceAmplification curve CtExpected presenceAmplification curve Ct
1MON87712SoybeanPlasmidYes+Yes+
2GTS-40-3-2SoybeanIRMMYes+No–
3DP356043SoybeanIRMMYes+No–
4DP305423SoybeanIRMMYes+No–
5DAS 68416-4SoybeanIRMMYes+No–
6A5547-127SoybeanAOCSYes+No–
7MON89788SoybeanAOCSYes+No–
8A2704-12SoybeanAOCSYes+No–
9MON87701SoybeanAOCSYes+No–
10CV127SoybeanAOCSYes+No–
11MON87705SoybeanAOCSYes+No–
12MON87769SoybeanAOCSYes+No–
13FG72SoybeanAOCSYes+No–
14MON87708SoybeanAOCSYes+No–
15DAS-81419-2SoybeanIRMMYes+No–
16DAS-44406-6SoybeanIRMMYes+No–
17MON87751SoybeanAOCSYes+No–
18Non-GM soybeanSoybeanAOCSYes+No–
19Non-GM soybeanSoybeanAOCSYes+No–
20GA21CornAOCSNo–No–
21TC1507CornIRMMNo–No–
22MON87460CornAOCSNo–No–
23MIR162CornAOCSNo–No–
24MIR604CornAOCSNo–No–
25MON88302RapeAOCSNo–No–
26GT73RapeAOCSNo–No–
27Rf2RapeAOCSNo–No–
28Ms8RapeAOCSNo–No–
29Ms1RapeAOCSNo–No–
3073496RapeIRMMNo–No–
31Rf1RapeAOCSNo–No–
32Topas 19/2RapeAOCSNo–No–
33Rf3RapeAOCSNo–No–
34T45RapeAOCSNo–No–
35AM04-1020PotatoIRMMNo–No–
36EH92-527-1PotatoIRMMNo–No–
37Corn negative sampleCornLab sampleNo–No–
38AlfalfaAlfalfaLab sampleNo–No–
39Black soybeanBlack soybeanLab sampleNo–No–
40Red beanRed beanLab sampleNo–No–
41Mung beanMung beanLab sampleNo–No–
42PeanutPeanutLab sampleNo–No–
43RiceRiceLab sampleNo–No–
44Genetically modified cottonseedCottonseedLab sampleNo–No–
45BarleyBarleyLab sampleNo–No–
46WheatWheatLab sampleNo–No–
Table 3.
Open in new tab

The specificity of the duplex real-time PCR

Serial numberEventSpeciesSourceSoybean endogenous lectin geneMON87712 event-specific gene
Expected presenceAmplification curve CtExpected presenceAmplification curve Ct
1MON87712SoybeanPlasmidYes+Yes+
2GTS-40-3-2SoybeanIRMMYes+No–
3DP356043SoybeanIRMMYes+No–
4DP305423SoybeanIRMMYes+No–
5DAS 68416-4SoybeanIRMMYes+No–
6A5547-127SoybeanAOCSYes+No–
7MON89788SoybeanAOCSYes+No–
8A2704-12SoybeanAOCSYes+No–
9MON87701SoybeanAOCSYes+No–
10CV127SoybeanAOCSYes+No–
11MON87705SoybeanAOCSYes+No–
12MON87769SoybeanAOCSYes+No–
13FG72SoybeanAOCSYes+No–
14MON87708SoybeanAOCSYes+No–
15DAS-81419-2SoybeanIRMMYes+No–
16DAS-44406-6SoybeanIRMMYes+No–
17MON87751SoybeanAOCSYes+No–
18Non-GM soybeanSoybeanAOCSYes+No–
19Non-GM soybeanSoybeanAOCSYes+No–
20GA21CornAOCSNo–No–
21TC1507CornIRMMNo–No–
22MON87460CornAOCSNo–No–
23MIR162CornAOCSNo–No–
24MIR604CornAOCSNo–No–
25MON88302RapeAOCSNo–No–
26GT73RapeAOCSNo–No–
27Rf2RapeAOCSNo–No–
28Ms8RapeAOCSNo–No–
29Ms1RapeAOCSNo–No–
3073496RapeIRMMNo–No–
31Rf1RapeAOCSNo–No–
32Topas 19/2RapeAOCSNo–No–
33Rf3RapeAOCSNo–No–
34T45RapeAOCSNo–No–
35AM04-1020PotatoIRMMNo–No–
36EH92-527-1PotatoIRMMNo–No–
37Corn negative sampleCornLab sampleNo–No–
38AlfalfaAlfalfaLab sampleNo–No–
39Black soybeanBlack soybeanLab sampleNo–No–
40Red beanRed beanLab sampleNo–No–
41Mung beanMung beanLab sampleNo–No–
42PeanutPeanutLab sampleNo–No–
43RiceRiceLab sampleNo–No–
44Genetically modified cottonseedCottonseedLab sampleNo–No–
45BarleyBarleyLab sampleNo–No–
46WheatWheatLab sampleNo–No–
Serial numberEventSpeciesSourceSoybean endogenous lectin geneMON87712 event-specific gene
Expected presenceAmplification curve CtExpected presenceAmplification curve Ct
1MON87712SoybeanPlasmidYes+Yes+
2GTS-40-3-2SoybeanIRMMYes+No–
3DP356043SoybeanIRMMYes+No–
4DP305423SoybeanIRMMYes+No–
5DAS 68416-4SoybeanIRMMYes+No–
6A5547-127SoybeanAOCSYes+No–
7MON89788SoybeanAOCSYes+No–
8A2704-12SoybeanAOCSYes+No–
9MON87701SoybeanAOCSYes+No–
10CV127SoybeanAOCSYes+No–
11MON87705SoybeanAOCSYes+No–
12MON87769SoybeanAOCSYes+No–
13FG72SoybeanAOCSYes+No–
14MON87708SoybeanAOCSYes+No–
15DAS-81419-2SoybeanIRMMYes+No–
16DAS-44406-6SoybeanIRMMYes+No–
17MON87751SoybeanAOCSYes+No–
18Non-GM soybeanSoybeanAOCSYes+No–
19Non-GM soybeanSoybeanAOCSYes+No–
20GA21CornAOCSNo–No–
21TC1507CornIRMMNo–No–
22MON87460CornAOCSNo–No–
23MIR162CornAOCSNo–No–
24MIR604CornAOCSNo–No–
25MON88302RapeAOCSNo–No–
26GT73RapeAOCSNo–No–
27Rf2RapeAOCSNo–No–
28Ms8RapeAOCSNo–No–
29Ms1RapeAOCSNo–No–
3073496RapeIRMMNo–No–
31Rf1RapeAOCSNo–No–
32Topas 19/2RapeAOCSNo–No–
33Rf3RapeAOCSNo–No–
34T45RapeAOCSNo–No–
35AM04-1020PotatoIRMMNo–No–
36EH92-527-1PotatoIRMMNo–No–
37Corn negative sampleCornLab sampleNo–No–
38AlfalfaAlfalfaLab sampleNo–No–
39Black soybeanBlack soybeanLab sampleNo–No–
40Red beanRed beanLab sampleNo–No–
41Mung beanMung beanLab sampleNo–No–
42PeanutPeanutLab sampleNo–No–
43RiceRiceLab sampleNo–No–
44Genetically modified cottonseedCottonseedLab sampleNo–No–
45BarleyBarleyLab sampleNo–No–
46WheatWheatLab sampleNo–No–
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