Enzyme Kinetic Parameter Estimates for Modern-Day and Ancestral Enzymes with Selected Substrates.
| Enzyme (substrate) . | KM (μM) . | kcat (1/s) . | kcat/KM (s−1M−1) . |
|---|---|---|---|
| Modern-day enzymes | |||
| TcCS1 (X) | 95.80 | 8.37E−05 | 0.87 |
| TcCS2 (3X) | 49.10 | 9.81E−05 | 2.00 |
| PcCS1 (X) | 95.38 | 1.52E−03 | 15.94 |
| PcCS2 (3X) | 677.00 | 9.33E−04 | 1.38 |
| Ancestral enzymes | |||
| TcAncCS1 (X) | 53.40 | 8.65E−05 | 1.62 |
| TcAncCS1 (3X) | 138.60 | 1.28E−04 | 0.92 |
| TcAncCS1 (7X) | 34.60 | 2.47E−04 | 7.20 |
| TcAncCS2 (X) | 4.14 | 3.39E−04 | 82.06 |
| TcAncCS2 (3X) | 154.00 | 3.32E−04 | 2.16 |
| PcAncCS1 (X) | 417.50 | 3.76E−04 | 0.90 |
| CsAncCS (7X) | 26.70 | 1.05E−03 | 39.33 |
| Enzyme (substrate) . | KM (μM) . | kcat (1/s) . | kcat/KM (s−1M−1) . |
|---|---|---|---|
| Modern-day enzymes | |||
| TcCS1 (X) | 95.80 | 8.37E−05 | 0.87 |
| TcCS2 (3X) | 49.10 | 9.81E−05 | 2.00 |
| PcCS1 (X) | 95.38 | 1.52E−03 | 15.94 |
| PcCS2 (3X) | 677.00 | 9.33E−04 | 1.38 |
| Ancestral enzymes | |||
| TcAncCS1 (X) | 53.40 | 8.65E−05 | 1.62 |
| TcAncCS1 (3X) | 138.60 | 1.28E−04 | 0.92 |
| TcAncCS1 (7X) | 34.60 | 2.47E−04 | 7.20 |
| TcAncCS2 (X) | 4.14 | 3.39E−04 | 82.06 |
| TcAncCS2 (3X) | 154.00 | 3.32E−04 | 2.16 |
| PcAncCS1 (X) | 417.50 | 3.76E−04 | 0.90 |
| CsAncCS (7X) | 26.70 | 1.05E−03 | 39.33 |
Enzyme Kinetic Parameter Estimates for Modern-Day and Ancestral Enzymes with Selected Substrates.
| Enzyme (substrate) . | KM (μM) . | kcat (1/s) . | kcat/KM (s−1M−1) . |
|---|---|---|---|
| Modern-day enzymes | |||
| TcCS1 (X) | 95.80 | 8.37E−05 | 0.87 |
| TcCS2 (3X) | 49.10 | 9.81E−05 | 2.00 |
| PcCS1 (X) | 95.38 | 1.52E−03 | 15.94 |
| PcCS2 (3X) | 677.00 | 9.33E−04 | 1.38 |
| Ancestral enzymes | |||
| TcAncCS1 (X) | 53.40 | 8.65E−05 | 1.62 |
| TcAncCS1 (3X) | 138.60 | 1.28E−04 | 0.92 |
| TcAncCS1 (7X) | 34.60 | 2.47E−04 | 7.20 |
| TcAncCS2 (X) | 4.14 | 3.39E−04 | 82.06 |
| TcAncCS2 (3X) | 154.00 | 3.32E−04 | 2.16 |
| PcAncCS1 (X) | 417.50 | 3.76E−04 | 0.90 |
| CsAncCS (7X) | 26.70 | 1.05E−03 | 39.33 |
| Enzyme (substrate) . | KM (μM) . | kcat (1/s) . | kcat/KM (s−1M−1) . |
|---|---|---|---|
| Modern-day enzymes | |||
| TcCS1 (X) | 95.80 | 8.37E−05 | 0.87 |
| TcCS2 (3X) | 49.10 | 9.81E−05 | 2.00 |
| PcCS1 (X) | 95.38 | 1.52E−03 | 15.94 |
| PcCS2 (3X) | 677.00 | 9.33E−04 | 1.38 |
| Ancestral enzymes | |||
| TcAncCS1 (X) | 53.40 | 8.65E−05 | 1.62 |
| TcAncCS1 (3X) | 138.60 | 1.28E−04 | 0.92 |
| TcAncCS1 (7X) | 34.60 | 2.47E−04 | 7.20 |
| TcAncCS2 (X) | 4.14 | 3.39E−04 | 82.06 |
| TcAncCS2 (3X) | 154.00 | 3.32E−04 | 2.16 |
| PcAncCS1 (X) | 417.50 | 3.76E−04 | 0.90 |
| CsAncCS (7X) | 26.70 | 1.05E−03 | 39.33 |
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