Table I.
Amino acid content in leaves following darkening for 6 d of an individual leaf (IDL) and an entire plant (DP)

Results are means of three replicates ± se and are expressed in μmol g−1 fresh weight. Dark treatments were compared with light samples by Student’s t test (*, significant at P < 0.05; ns, not significant). n/a, Not applicable.

Amino AcidLightDPIDL
Ammonium0.80 ± 0.420.18 ± 0.05 ns0.54 ± 0.60 ns
Ala0.64 ± 0.111.03 ± 0.17*0.26 ± 0.04*
Arg0.03 ± 0.062.44 ± 0.06*0.33 ± 0.10*
Asn0.34 ± 0.0414.44 ± 1.13*0.99 ± 0.62 ns
Asp1.85 ± 0.200.85 ± 0.25*0.22 ± 0.04*
Cysn/an/an/a
γ-Aminobutyric acid0.00 ± 0.000.36 ± 0.03*0.17 ± 0.05*
Gln2.11 ± 0.192.93 ± 0.45 ns0.67 ± 0.27*
Glu2.15 ± 0.074.40 ± 0.34*1.86 ± 0.05*
Gly0.27 ± 0.070.00 ± 0.00*0.03 ± 0.03*
His0.00 ± 0.000.85 ± 0.03*0.05 ± 0.08 ns
Ile0.00 ± 0.000.99 ± 0.17*0.05 ± 0.05 ns
Leu0.11 ± 0.000.54 ± 0.07*0.22 ± 0.02*
Lys0.05 ± 0.090.47 ± 0.05*0.26 ± 0.03 ns
Metn/an/an/a
Phe0.00 ± 0.002.06 ± 0.08*0.12 ± 0.02*
Pro0.79 ± 0.380.06 ± 0.02 ns0.07 ± 0.01 ns
Ser1.30 ± 0.222.04 ± 0.40 ns0.88 ± 0.15 ns
Thr0.60 ± 0.141.16 ± 0.38 ns0.37 ± 0.08 ns
Trp0.00 ± 0.000.88 ± 0.02*0.15 ± 0.04*
Tyr0.01 ± 0.020.40 ± 0.04*0.08 ± 0.01*
Val0.10 ± 0.002.05 ± 0.28*0.19 ± 0.02*
Total11.1738.147.51
Amino AcidLightDPIDL
Ammonium0.80 ± 0.420.18 ± 0.05 ns0.54 ± 0.60 ns
Ala0.64 ± 0.111.03 ± 0.17*0.26 ± 0.04*
Arg0.03 ± 0.062.44 ± 0.06*0.33 ± 0.10*
Asn0.34 ± 0.0414.44 ± 1.13*0.99 ± 0.62 ns
Asp1.85 ± 0.200.85 ± 0.25*0.22 ± 0.04*
Cysn/an/an/a
γ-Aminobutyric acid0.00 ± 0.000.36 ± 0.03*0.17 ± 0.05*
Gln2.11 ± 0.192.93 ± 0.45 ns0.67 ± 0.27*
Glu2.15 ± 0.074.40 ± 0.34*1.86 ± 0.05*
Gly0.27 ± 0.070.00 ± 0.00*0.03 ± 0.03*
His0.00 ± 0.000.85 ± 0.03*0.05 ± 0.08 ns
Ile0.00 ± 0.000.99 ± 0.17*0.05 ± 0.05 ns
Leu0.11 ± 0.000.54 ± 0.07*0.22 ± 0.02*
Lys0.05 ± 0.090.47 ± 0.05*0.26 ± 0.03 ns
Metn/an/an/a
Phe0.00 ± 0.002.06 ± 0.08*0.12 ± 0.02*
Pro0.79 ± 0.380.06 ± 0.02 ns0.07 ± 0.01 ns
Ser1.30 ± 0.222.04 ± 0.40 ns0.88 ± 0.15 ns
Thr0.60 ± 0.141.16 ± 0.38 ns0.37 ± 0.08 ns
Trp0.00 ± 0.000.88 ± 0.02*0.15 ± 0.04*
Tyr0.01 ± 0.020.40 ± 0.04*0.08 ± 0.01*
Val0.10 ± 0.002.05 ± 0.28*0.19 ± 0.02*
Total11.1738.147.51
Table I.
Amino acid content in leaves following darkening for 6 d of an individual leaf (IDL) and an entire plant (DP)

Results are means of three replicates ± se and are expressed in μmol g−1 fresh weight. Dark treatments were compared with light samples by Student’s t test (*, significant at P < 0.05; ns, not significant). n/a, Not applicable.

Amino AcidLightDPIDL
Ammonium0.80 ± 0.420.18 ± 0.05 ns0.54 ± 0.60 ns
Ala0.64 ± 0.111.03 ± 0.17*0.26 ± 0.04*
Arg0.03 ± 0.062.44 ± 0.06*0.33 ± 0.10*
Asn0.34 ± 0.0414.44 ± 1.13*0.99 ± 0.62 ns
Asp1.85 ± 0.200.85 ± 0.25*0.22 ± 0.04*
Cysn/an/an/a
γ-Aminobutyric acid0.00 ± 0.000.36 ± 0.03*0.17 ± 0.05*
Gln2.11 ± 0.192.93 ± 0.45 ns0.67 ± 0.27*
Glu2.15 ± 0.074.40 ± 0.34*1.86 ± 0.05*
Gly0.27 ± 0.070.00 ± 0.00*0.03 ± 0.03*
His0.00 ± 0.000.85 ± 0.03*0.05 ± 0.08 ns
Ile0.00 ± 0.000.99 ± 0.17*0.05 ± 0.05 ns
Leu0.11 ± 0.000.54 ± 0.07*0.22 ± 0.02*
Lys0.05 ± 0.090.47 ± 0.05*0.26 ± 0.03 ns
Metn/an/an/a
Phe0.00 ± 0.002.06 ± 0.08*0.12 ± 0.02*
Pro0.79 ± 0.380.06 ± 0.02 ns0.07 ± 0.01 ns
Ser1.30 ± 0.222.04 ± 0.40 ns0.88 ± 0.15 ns
Thr0.60 ± 0.141.16 ± 0.38 ns0.37 ± 0.08 ns
Trp0.00 ± 0.000.88 ± 0.02*0.15 ± 0.04*
Tyr0.01 ± 0.020.40 ± 0.04*0.08 ± 0.01*
Val0.10 ± 0.002.05 ± 0.28*0.19 ± 0.02*
Total11.1738.147.51
Amino AcidLightDPIDL
Ammonium0.80 ± 0.420.18 ± 0.05 ns0.54 ± 0.60 ns
Ala0.64 ± 0.111.03 ± 0.17*0.26 ± 0.04*
Arg0.03 ± 0.062.44 ± 0.06*0.33 ± 0.10*
Asn0.34 ± 0.0414.44 ± 1.13*0.99 ± 0.62 ns
Asp1.85 ± 0.200.85 ± 0.25*0.22 ± 0.04*
Cysn/an/an/a
γ-Aminobutyric acid0.00 ± 0.000.36 ± 0.03*0.17 ± 0.05*
Gln2.11 ± 0.192.93 ± 0.45 ns0.67 ± 0.27*
Glu2.15 ± 0.074.40 ± 0.34*1.86 ± 0.05*
Gly0.27 ± 0.070.00 ± 0.00*0.03 ± 0.03*
His0.00 ± 0.000.85 ± 0.03*0.05 ± 0.08 ns
Ile0.00 ± 0.000.99 ± 0.17*0.05 ± 0.05 ns
Leu0.11 ± 0.000.54 ± 0.07*0.22 ± 0.02*
Lys0.05 ± 0.090.47 ± 0.05*0.26 ± 0.03 ns
Metn/an/an/a
Phe0.00 ± 0.002.06 ± 0.08*0.12 ± 0.02*
Pro0.79 ± 0.380.06 ± 0.02 ns0.07 ± 0.01 ns
Ser1.30 ± 0.222.04 ± 0.40 ns0.88 ± 0.15 ns
Thr0.60 ± 0.141.16 ± 0.38 ns0.37 ± 0.08 ns
Trp0.00 ± 0.000.88 ± 0.02*0.15 ± 0.04*
Tyr0.01 ± 0.020.40 ± 0.04*0.08 ± 0.01*
Val0.10 ± 0.002.05 ± 0.28*0.19 ± 0.02*
Total11.1738.147.51
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