Table I. Open in new tab Peptides with...

Spot No.

Peptide Sequence

Posttranslational Modification

Calculated M _r

m/z

Protein Sequence Coverage

Score

⁶³AVVDAAPIQTTK⁷⁴

Acetyl

1,254.68

628.32 (+2)

⁵²⁴GG_oxMpTSHAAVVAR⁵³⁵

Thr-527

1,251.54

626.78 (+2)

³⁰⁸GTAVNVQCMVFGNMGNTSGTGVLFTR³³³

2,749.26

917.43 (+3)

101

⁵⁰¹VRAETSPEDVGGMHAAVGIL⁵²⁰

2,024.00

1,013.02 (+2)

126

⁶³AVVDAAPIQTTKKRVF⁷⁸

Acetyl

1,785.01

893.52 (+2)

⁵²⁴GG_oxMpTSHAAVVAR⁵³⁵

Thr-527

1,251.54

626.78 (+2)

⁵²⁴GG_oxMTSHAAVVAR⁵³⁵

1,172.58

586.79 (+2)

113

³⁰⁸GTAVNVQCMVFGNMGNTSGTGVLFTR³³³

2,749.26

917.43 (+2)

116

⁵⁰¹VRAETSPEDVGGMHAAVGIL5²⁰

2,024.00

675.67 (+2)

⁶³AVVDAAPIQTTK⁷⁴

Acetyl

1,254.68

628.35 (+2)

⁶³AVVDAAPIQTTK⁷⁴

1,212.67

607.34 (+2)

⁵²⁴GGMpTSHAAVVAR⁵³⁵

Thr-527

1,235.55

618.79 (+2)

³⁰⁸GpTAVNVQCMVFGNMGNTSGTGVLFTR³³³

Thr-309

2,740.22

914.42 (+3)

⁵⁰³AETSPEDVGGMHAAVGILTER⁵²³

2,155.02

719.34 (+3)

107

³⁰⁸GTAVNVQCMVFGNMGNTSGTGVLFTR³³³

2,749.26

1,375.65 (+2)

157

⁵⁰³AETSPEDVGGMHAAVGILTER⁵²³

1,785.01

893.52 (+2)

⁶³AVVDAAPIQTTKKRVF⁷⁸

Acetyl

2,234.99

1,118.51 (+2)

⁵⁰³AETpSPEDVGGMHAAVGILTER⁵²³

Ser-506

1,235.55

618.78 (+2)

102

⁵⁰³AETSPEDVGGMHAAVGILTER⁵²³

2,139.03

714.01 (+3)

118

⁵²⁴GG_oxMpTSHAAVVAR⁵³⁵

Thr-527

1,172.58

586.79 (+2)

⁵²⁴GG_oxMTSHAAVVAR⁵³⁵

1,251.54

626.78 (+2)

⁵²⁴GGMTpSHAAVVAR⁵³⁵

Ser-528

2,733.27

1,367.64 (+2)

151

³⁰⁸GTAVNVQCMVFGNMGNTSGTGVLFTR³³³

2,155.02

719.32 (+3)

146

⁶³AVVDAAPIQTTK⁷⁴

1,212.67

607.33 (+2)

⁵⁰³AETpSPEDVGGMHAAVGILTER⁵²³

Ser-506′

2,234.99

1,118.51 (+2)

⁵⁰³AETSPEDVGGMHAAVGILTER⁵²³

2,139.03

714.01 (+3)

121

⁵²⁴GGMpTSHAAVVAR⁵³⁵

Thr-527

1,235.55

618.78 (+2)

100

⁵²⁴GG_oxMTpSHAAVVAR⁵³⁵

Ser-528

1,251.54

626.78 (+2)

⁵²⁴GGMpTpSHAAVVAR⁵³⁵

Thr-527, Ser-528

1,373.52

687.73 (+2)

³⁰⁸GTAVNVQCMVFGNMGNTSGTGVLFTR³³³

2,733.27

912.09 (+3)

⁶³AVVDAAPIQTTK⁷⁴

Thr-527

1,212.67

607.34 (+2)

⁵²⁴GG_oxMpTSHAAVVAR⁵³⁵

1235.55

618.78 (+2)

⁵⁰³AETSPEDVGGMHAAVGILTER⁵²³

2,155.02

719.34 (+3)

123

³⁰⁸GTAVNVQCMVFGNMGNTSGTGVLFTR³³³

2,749.26

917.43 (+3)

113

Table I.

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Peptides with different posttranslational modifications and their unmodified counterparts identified in the protein spots corresponding to maize C₄PPDK

ox and p, Oxidation and phosphorylation of amino acids, respectively; acetyl, N-terminal acetylation of a peptide.

Spot No.	Peptide Sequence	Posttranslational Modification	Calculated M _r	m/z	Protein Sequence Coverage	Score
					%
1	⁶³AVVDAAPIQTTK⁷⁴	Acetyl	1,254.68	628.32 (+2)	72	51
	⁵²⁴GG_oxMpTSHAAVVAR⁵³⁵	Thr-527	1,251.54	626.78 (+2)		90
	³⁰⁸GTAVNVQCMVFGNMGNTSGTGVLFTR³³³		2,749.26	917.43 (+3)		101
	⁵⁰¹VRAETSPEDVGGMHAAVGIL⁵²⁰		2,024.00	1,013.02 (+2)		126
2	⁶³AVVDAAPIQTTKKRVF⁷⁸	Acetyl	1,785.01	893.52 (+2)	88	98
	⁵²⁴GG_oxMpTSHAAVVAR⁵³⁵	Thr-527	1,251.54	626.78 (+2)		94
	⁵²⁴GG_oxMTSHAAVVAR⁵³⁵		1,172.58	586.79 (+2)		113
	³⁰⁸GTAVNVQCMVFGNMGNTSGTGVLFTR³³³		2,749.26	917.43 (+2)		116
	⁵⁰¹VRAETSPEDVGGMHAAVGIL5²⁰		2,024.00	675.67 (+2)		81
3	⁶³AVVDAAPIQTTK⁷⁴	Acetyl	1,254.68	628.35 (+2)	92	72
	⁶³AVVDAAPIQTTK⁷⁴		1,212.67	607.34 (+2)		57
	⁵²⁴GGMpTSHAAVVAR⁵³⁵	Thr-527	1,235.55	618.79 (+2)		94
	³⁰⁸GpTAVNVQCMVFGNMGNTSGTGVLFTR³³³	Thr-309	2,740.22	914.42 (+3)		62
	⁵⁰³AETSPEDVGGMHAAVGILTER⁵²³		2,155.02	719.34 (+3)		107
	³⁰⁸GTAVNVQCMVFGNMGNTSGTGVLFTR³³³		2,749.26	1,375.65 (+2)		157
4	⁵⁰³AETSPEDVGGMHAAVGILTER⁵²³		1,785.01	893.52 (+2)	94	93
	⁶³AVVDAAPIQTTKKRVF⁷⁸	Acetyl	2,234.99	1,118.51 (+2)		83
	⁵⁰³AETpSPEDVGGMHAAVGILTER⁵²³	Ser-506	1,235.55	618.78 (+2)		102
	⁵⁰³AETSPEDVGGMHAAVGILTER⁵²³		2,139.03	714.01 (+3)		118
	⁵²⁴GG_oxMpTSHAAVVAR⁵³⁵	Thr-527	1,172.58	586.79 (+2)		98
	⁵²⁴GG_oxMTSHAAVVAR⁵³⁵		1,251.54	626.78 (+2)		49
	⁵²⁴GGMTpSHAAVVAR⁵³⁵	Ser-528	2,733.27	1,367.64 (+2)		151
	³⁰⁸GTAVNVQCMVFGNMGNTSGTGVLFTR³³³		2,155.02	719.32 (+3)		146
5	⁶³AVVDAAPIQTTK⁷⁴		1,212.67	607.33 (+2)	97	60
	⁵⁰³AETpSPEDVGGMHAAVGILTER⁵²³	Ser-506′	2,234.99	1,118.51 (+2)		77
	⁵⁰³AETSPEDVGGMHAAVGILTER⁵²³		2,139.03	714.01 (+3)		121
	⁵²⁴GGMpTSHAAVVAR⁵³⁵	Thr-527	1,235.55	618.78 (+2)		100
	⁵²⁴GG_oxMTpSHAAVVAR⁵³⁵	Ser-528	1,251.54	626.78 (+2)		83
	⁵²⁴GGMpTpSHAAVVAR⁵³⁵	Thr-527, Ser-528	1,373.52	687.73 (+2)		43
	³⁰⁸GTAVNVQCMVFGNMGNTSGTGVLFTR³³³		2,733.27	912.09 (+3)		57
6	⁶³AVVDAAPIQTTK⁷⁴	Thr-527	1,212.67	607.34 (+2)	98	60
	⁵²⁴GG_oxMpTSHAAVVAR⁵³⁵		1235.55	618.78 (+2)		99
	⁵⁰³AETSPEDVGGMHAAVGILTER⁵²³		2,155.02	719.34 (+3)		123
	³⁰⁸GTAVNVQCMVFGNMGNTSGTGVLFTR³³³		2,749.26	917.43 (+3)		113

Spot No.	Peptide Sequence	Posttranslational Modification	Calculated M _r	m/z	Protein Sequence Coverage	Score
					%
1	⁶³AVVDAAPIQTTK⁷⁴	Acetyl	1,254.68	628.32 (+2)	72	51
	⁵²⁴GG_oxMpTSHAAVVAR⁵³⁵	Thr-527	1,251.54	626.78 (+2)		90
	³⁰⁸GTAVNVQCMVFGNMGNTSGTGVLFTR³³³		2,749.26	917.43 (+3)		101
	⁵⁰¹VRAETSPEDVGGMHAAVGIL⁵²⁰		2,024.00	1,013.02 (+2)		126
2	⁶³AVVDAAPIQTTKKRVF⁷⁸	Acetyl	1,785.01	893.52 (+2)	88	98
	⁵²⁴GG_oxMpTSHAAVVAR⁵³⁵	Thr-527	1,251.54	626.78 (+2)		94
	⁵²⁴GG_oxMTSHAAVVAR⁵³⁵		1,172.58	586.79 (+2)		113
	³⁰⁸GTAVNVQCMVFGNMGNTSGTGVLFTR³³³		2,749.26	917.43 (+2)		116
	⁵⁰¹VRAETSPEDVGGMHAAVGIL5²⁰		2,024.00	675.67 (+2)		81
3	⁶³AVVDAAPIQTTK⁷⁴	Acetyl	1,254.68	628.35 (+2)	92	72
	⁶³AVVDAAPIQTTK⁷⁴		1,212.67	607.34 (+2)		57
	⁵²⁴GGMpTSHAAVVAR⁵³⁵	Thr-527	1,235.55	618.79 (+2)		94
	³⁰⁸GpTAVNVQCMVFGNMGNTSGTGVLFTR³³³	Thr-309	2,740.22	914.42 (+3)		62
	⁵⁰³AETSPEDVGGMHAAVGILTER⁵²³		2,155.02	719.34 (+3)		107
	³⁰⁸GTAVNVQCMVFGNMGNTSGTGVLFTR³³³		2,749.26	1,375.65 (+2)		157
4	⁵⁰³AETSPEDVGGMHAAVGILTER⁵²³		1,785.01	893.52 (+2)	94	93
	⁶³AVVDAAPIQTTKKRVF⁷⁸	Acetyl	2,234.99	1,118.51 (+2)		83
	⁵⁰³AETpSPEDVGGMHAAVGILTER⁵²³	Ser-506	1,235.55	618.78 (+2)		102
	⁵⁰³AETSPEDVGGMHAAVGILTER⁵²³		2,139.03	714.01 (+3)		118
	⁵²⁴GG_oxMpTSHAAVVAR⁵³⁵	Thr-527	1,172.58	586.79 (+2)		98
	⁵²⁴GG_oxMTSHAAVVAR⁵³⁵		1,251.54	626.78 (+2)		49
	⁵²⁴GGMTpSHAAVVAR⁵³⁵	Ser-528	2,733.27	1,367.64 (+2)		151
	³⁰⁸GTAVNVQCMVFGNMGNTSGTGVLFTR³³³		2,155.02	719.32 (+3)		146
5	⁶³AVVDAAPIQTTK⁷⁴		1,212.67	607.33 (+2)	97	60
	⁵⁰³AETpSPEDVGGMHAAVGILTER⁵²³	Ser-506′	2,234.99	1,118.51 (+2)		77
	⁵⁰³AETSPEDVGGMHAAVGILTER⁵²³		2,139.03	714.01 (+3)		121
	⁵²⁴GGMpTSHAAVVAR⁵³⁵	Thr-527	1,235.55	618.78 (+2)		100
	⁵²⁴GG_oxMTpSHAAVVAR⁵³⁵	Ser-528	1,251.54	626.78 (+2)		83
	⁵²⁴GGMpTpSHAAVVAR⁵³⁵	Thr-527, Ser-528	1,373.52	687.73 (+2)		43
	³⁰⁸GTAVNVQCMVFGNMGNTSGTGVLFTR³³³		2,733.27	912.09 (+3)		57
6	⁶³AVVDAAPIQTTK⁷⁴	Thr-527	1,212.67	607.34 (+2)	98	60
	⁵²⁴GG_oxMpTSHAAVVAR⁵³⁵		1235.55	618.78 (+2)		99
	⁵⁰³AETSPEDVGGMHAAVGILTER⁵²³		2,155.02	719.34 (+3)		123
	³⁰⁸GTAVNVQCMVFGNMGNTSGTGVLFTR³³³		2,749.26	917.43 (+3)		113

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