Table II.
Open in new tab
Fatty acid profiles of leaf total fatty acid (L), leaf TAG (L-TAG), root total fatty acid (R), and root TAG (R-TAG) from material harvested 35 DAS

Values represent mean percentages of total fatty acids ± sd from separate analysis (n = 6 for total fatty acid profiling in both leaves and roots, n = 4 for TAG profiling in both leaves and roots). Parentheses show P values for statistical significance between the transgenic line and the wild type calculated by Student’s two-tailed t test. nd, Not detected; ns, not significant.

ConstructC16:0C16:1C16:3C18:0C18:1C18:2C18:3C20:1C22:0C24:0C24:1
Wild type (L)17.2 ± 0.94 ± 0.211.3 ± 0.9nd1.6 ± 0.111.8 ± 1.154.2 ± 4.2ndndndnd
Vector control (L)16.6 ± 0.4
(ns)3.6 ± 0.1
(P = 0.0126)11.5 ± 0.5
(ns)nd1.5 ± 0.1
(ns)12.2 ± 0.3
(ns)54.6 ± 1.3
(ns)ndndndnd
D1 (L)16.4 ± 1.6
(ns)3.5 ± 0.6
(ns)11.5 ± 1.6
(ns)nd1.6 ± 0.4
(ns)12.6 ± 1.9
(ns)54.4 ± 7
(ns)ndndndnd
D1o0-0 (L)17 ± 1.3
(ns)3.5 ± 0.4
(P = 0.0173)11.8 ± 1.2
(ns)nd1.5 ± 0.3
(ns)11.2 ± 0.8
(ns)54.6 ± 5.5
(ns)ndndndnd
D1o1-1 (L)16.8 ± 0.7
(ns)3.5 ± 0.4
(ns)13.3 ± 1
(P = 0.0106)nd4.6 ± 0.4
(P = 3.1 E-09)12.5 ± 1.1
(ns)49.2 ± 3.1
(P = 0.0255)ndndndnd
D1o1-3 (L)15.7 ± 1.9
(ns)3.1 ± 0.4
(P = 0.0011)10.9 ± 1.5
(ns)nd4.1 ± 0.3
(P = 1.5 E-09)11.6 ± 2.4
(ns)54.5 ± 6.7
(ns)ndndndnd
D1o3-1 (L)14.8 ± 0.5
(P = 0.0002)3.1 ± 0.1
(P = 1.1 E-05)11 ± 0.9
(ns)nd4 ± 0.2
(P = 1.9 E-10)19.7 ± 1.4
(P = 1.04 E-06)47.4 ± 4.8
(P = 0.0197)ndndndnd
D1o3-3 (L)14 ± 0.3
(P = 6.2 E-06)2.1 ± 0.2
(P = 1.3 E-08)8.8 ± 0.3
(P = 6.4 E-05)nd12.2 ± 0.5
(P = 2.7 E-13)29 ± 0.3
(P = 6.2 E-12)32.4 ± 0.9
(P = 1.8 E-07)0.2 ± 0.040.3 ± 0.070.5 ± 0.020.4 ± 0.03
Wild type (R)22.8 ± 0.50.3 ± 0.02nd1 ± 0.072.6 ± 0.239.2 ± 1.328.5 ± 1.6nd3.3 ± 0.32.5 ± 0.2nd
D1o3-3 (R)15.7 ± 0.3
(P = 2.0 E-11)0.4 ± 0.1
(ns)nd2.8 ± 0.1 (P = 9.1 E-11)12.8 ± 0.6
(P = 6 E-11)45.9 ± 1
(P = 1.5 E-06)17.8 ± 0.7
(P = 3.2 E-08)nd2.5 ± 0.1
(P = 9.8 E-05)1.6 ± 0.1
(P = 8.5 E-07)0.5 ± 0.1
Wild type (L-TAG)37.0 ± 3.4ndndnd8.8 ± 2.223.7 ± 5.130.5 ± 1.4ndndndnd
D1o3-3 (L-TAG)12.5 ± 0.05
(P = 7.5 E-06)0.9 ± 0.011.6 ± 0.031.6 ± 0.0114.5 ± 0.01
(P = 0.0022)35.6 ± 0.2
(P = 0.0035)29.7 ± 0.09
(P = 0.2936)0.7 ± 0.070.8 ± 0.020.8 ± 0.11.2 ± 0.08
Wild type (R-TAG)ndndndndndndndndndndnd
D1o3-3 (R-TAG)15.1 ± 0.10.7 ± 0.02nd5.7 ± 0.221.6 ± 0.237.1 ± 0.414.1 ± 0.20.3 ± 0.033.1 ± 0.11.4 ± 0.10.8 ± 0.02
ConstructC16:0C16:1C16:3C18:0C18:1C18:2C18:3C20:1C22:0C24:0C24:1
Wild type (L)17.2 ± 0.94 ± 0.211.3 ± 0.9nd1.6 ± 0.111.8 ± 1.154.2 ± 4.2ndndndnd
Vector control (L)16.6 ± 0.4
(ns)3.6 ± 0.1
(P = 0.0126)11.5 ± 0.5
(ns)nd1.5 ± 0.1
(ns)12.2 ± 0.3
(ns)54.6 ± 1.3
(ns)ndndndnd
D1 (L)16.4 ± 1.6
(ns)3.5 ± 0.6
(ns)11.5 ± 1.6
(ns)nd1.6 ± 0.4
(ns)12.6 ± 1.9
(ns)54.4 ± 7
(ns)ndndndnd
D1o0-0 (L)17 ± 1.3
(ns)3.5 ± 0.4
(P = 0.0173)11.8 ± 1.2
(ns)nd1.5 ± 0.3
(ns)11.2 ± 0.8
(ns)54.6 ± 5.5
(ns)ndndndnd
D1o1-1 (L)16.8 ± 0.7
(ns)3.5 ± 0.4
(ns)13.3 ± 1
(P = 0.0106)nd4.6 ± 0.4
(P = 3.1 E-09)12.5 ± 1.1
(ns)49.2 ± 3.1
(P = 0.0255)ndndndnd
D1o1-3 (L)15.7 ± 1.9
(ns)3.1 ± 0.4
(P = 0.0011)10.9 ± 1.5
(ns)nd4.1 ± 0.3
(P = 1.5 E-09)11.6 ± 2.4
(ns)54.5 ± 6.7
(ns)ndndndnd
D1o3-1 (L)14.8 ± 0.5
(P = 0.0002)3.1 ± 0.1
(P = 1.1 E-05)11 ± 0.9
(ns)nd4 ± 0.2
(P = 1.9 E-10)19.7 ± 1.4
(P = 1.04 E-06)47.4 ± 4.8
(P = 0.0197)ndndndnd
D1o3-3 (L)14 ± 0.3
(P = 6.2 E-06)2.1 ± 0.2
(P = 1.3 E-08)8.8 ± 0.3
(P = 6.4 E-05)nd12.2 ± 0.5
(P = 2.7 E-13)29 ± 0.3
(P = 6.2 E-12)32.4 ± 0.9
(P = 1.8 E-07)0.2 ± 0.040.3 ± 0.070.5 ± 0.020.4 ± 0.03
Wild type (R)22.8 ± 0.50.3 ± 0.02nd1 ± 0.072.6 ± 0.239.2 ± 1.328.5 ± 1.6nd3.3 ± 0.32.5 ± 0.2nd
D1o3-3 (R)15.7 ± 0.3
(P = 2.0 E-11)0.4 ± 0.1
(ns)nd2.8 ± 0.1 (P = 9.1 E-11)12.8 ± 0.6
(P = 6 E-11)45.9 ± 1
(P = 1.5 E-06)17.8 ± 0.7
(P = 3.2 E-08)nd2.5 ± 0.1
(P = 9.8 E-05)1.6 ± 0.1
(P = 8.5 E-07)0.5 ± 0.1
Wild type (L-TAG)37.0 ± 3.4ndndnd8.8 ± 2.223.7 ± 5.130.5 ± 1.4ndndndnd
D1o3-3 (L-TAG)12.5 ± 0.05
(P = 7.5 E-06)0.9 ± 0.011.6 ± 0.031.6 ± 0.0114.5 ± 0.01
(P = 0.0022)35.6 ± 0.2
(P = 0.0035)29.7 ± 0.09
(P = 0.2936)0.7 ± 0.070.8 ± 0.020.8 ± 0.11.2 ± 0.08
Wild type (R-TAG)ndndndndndndndndndndnd
D1o3-3 (R-TAG)15.1 ± 0.10.7 ± 0.02nd5.7 ± 0.221.6 ± 0.237.1 ± 0.414.1 ± 0.20.3 ± 0.033.1 ± 0.11.4 ± 0.10.8 ± 0.02
Table II.
Open in new tab
Fatty acid profiles of leaf total fatty acid (L), leaf TAG (L-TAG), root total fatty acid (R), and root TAG (R-TAG) from material harvested 35 DAS

Values represent mean percentages of total fatty acids ± sd from separate analysis (n = 6 for total fatty acid profiling in both leaves and roots, n = 4 for TAG profiling in both leaves and roots). Parentheses show P values for statistical significance between the transgenic line and the wild type calculated by Student’s two-tailed t test. nd, Not detected; ns, not significant.

ConstructC16:0C16:1C16:3C18:0C18:1C18:2C18:3C20:1C22:0C24:0C24:1
Wild type (L)17.2 ± 0.94 ± 0.211.3 ± 0.9nd1.6 ± 0.111.8 ± 1.154.2 ± 4.2ndndndnd
Vector control (L)16.6 ± 0.4
(ns)3.6 ± 0.1
(P = 0.0126)11.5 ± 0.5
(ns)nd1.5 ± 0.1
(ns)12.2 ± 0.3
(ns)54.6 ± 1.3
(ns)ndndndnd
D1 (L)16.4 ± 1.6
(ns)3.5 ± 0.6
(ns)11.5 ± 1.6
(ns)nd1.6 ± 0.4
(ns)12.6 ± 1.9
(ns)54.4 ± 7
(ns)ndndndnd
D1o0-0 (L)17 ± 1.3
(ns)3.5 ± 0.4
(P = 0.0173)11.8 ± 1.2
(ns)nd1.5 ± 0.3
(ns)11.2 ± 0.8
(ns)54.6 ± 5.5
(ns)ndndndnd
D1o1-1 (L)16.8 ± 0.7
(ns)3.5 ± 0.4
(ns)13.3 ± 1
(P = 0.0106)nd4.6 ± 0.4
(P = 3.1 E-09)12.5 ± 1.1
(ns)49.2 ± 3.1
(P = 0.0255)ndndndnd
D1o1-3 (L)15.7 ± 1.9
(ns)3.1 ± 0.4
(P = 0.0011)10.9 ± 1.5
(ns)nd4.1 ± 0.3
(P = 1.5 E-09)11.6 ± 2.4
(ns)54.5 ± 6.7
(ns)ndndndnd
D1o3-1 (L)14.8 ± 0.5
(P = 0.0002)3.1 ± 0.1
(P = 1.1 E-05)11 ± 0.9
(ns)nd4 ± 0.2
(P = 1.9 E-10)19.7 ± 1.4
(P = 1.04 E-06)47.4 ± 4.8
(P = 0.0197)ndndndnd
D1o3-3 (L)14 ± 0.3
(P = 6.2 E-06)2.1 ± 0.2
(P = 1.3 E-08)8.8 ± 0.3
(P = 6.4 E-05)nd12.2 ± 0.5
(P = 2.7 E-13)29 ± 0.3
(P = 6.2 E-12)32.4 ± 0.9
(P = 1.8 E-07)0.2 ± 0.040.3 ± 0.070.5 ± 0.020.4 ± 0.03
Wild type (R)22.8 ± 0.50.3 ± 0.02nd1 ± 0.072.6 ± 0.239.2 ± 1.328.5 ± 1.6nd3.3 ± 0.32.5 ± 0.2nd
D1o3-3 (R)15.7 ± 0.3
(P = 2.0 E-11)0.4 ± 0.1
(ns)nd2.8 ± 0.1 (P = 9.1 E-11)12.8 ± 0.6
(P = 6 E-11)45.9 ± 1
(P = 1.5 E-06)17.8 ± 0.7
(P = 3.2 E-08)nd2.5 ± 0.1
(P = 9.8 E-05)1.6 ± 0.1
(P = 8.5 E-07)0.5 ± 0.1
Wild type (L-TAG)37.0 ± 3.4ndndnd8.8 ± 2.223.7 ± 5.130.5 ± 1.4ndndndnd
D1o3-3 (L-TAG)12.5 ± 0.05
(P = 7.5 E-06)0.9 ± 0.011.6 ± 0.031.6 ± 0.0114.5 ± 0.01
(P = 0.0022)35.6 ± 0.2
(P = 0.0035)29.7 ± 0.09
(P = 0.2936)0.7 ± 0.070.8 ± 0.020.8 ± 0.11.2 ± 0.08
Wild type (R-TAG)ndndndndndndndndndndnd
D1o3-3 (R-TAG)15.1 ± 0.10.7 ± 0.02nd5.7 ± 0.221.6 ± 0.237.1 ± 0.414.1 ± 0.20.3 ± 0.033.1 ± 0.11.4 ± 0.10.8 ± 0.02
ConstructC16:0C16:1C16:3C18:0C18:1C18:2C18:3C20:1C22:0C24:0C24:1
Wild type (L)17.2 ± 0.94 ± 0.211.3 ± 0.9nd1.6 ± 0.111.8 ± 1.154.2 ± 4.2ndndndnd
Vector control (L)16.6 ± 0.4
(ns)3.6 ± 0.1
(P = 0.0126)11.5 ± 0.5
(ns)nd1.5 ± 0.1
(ns)12.2 ± 0.3
(ns)54.6 ± 1.3
(ns)ndndndnd
D1 (L)16.4 ± 1.6
(ns)3.5 ± 0.6
(ns)11.5 ± 1.6
(ns)nd1.6 ± 0.4
(ns)12.6 ± 1.9
(ns)54.4 ± 7
(ns)ndndndnd
D1o0-0 (L)17 ± 1.3
(ns)3.5 ± 0.4
(P = 0.0173)11.8 ± 1.2
(ns)nd1.5 ± 0.3
(ns)11.2 ± 0.8
(ns)54.6 ± 5.5
(ns)ndndndnd
D1o1-1 (L)16.8 ± 0.7
(ns)3.5 ± 0.4
(ns)13.3 ± 1
(P = 0.0106)nd4.6 ± 0.4
(P = 3.1 E-09)12.5 ± 1.1
(ns)49.2 ± 3.1
(P = 0.0255)ndndndnd
D1o1-3 (L)15.7 ± 1.9
(ns)3.1 ± 0.4
(P = 0.0011)10.9 ± 1.5
(ns)nd4.1 ± 0.3
(P = 1.5 E-09)11.6 ± 2.4
(ns)54.5 ± 6.7
(ns)ndndndnd
D1o3-1 (L)14.8 ± 0.5
(P = 0.0002)3.1 ± 0.1
(P = 1.1 E-05)11 ± 0.9
(ns)nd4 ± 0.2
(P = 1.9 E-10)19.7 ± 1.4
(P = 1.04 E-06)47.4 ± 4.8
(P = 0.0197)ndndndnd
D1o3-3 (L)14 ± 0.3
(P = 6.2 E-06)2.1 ± 0.2
(P = 1.3 E-08)8.8 ± 0.3
(P = 6.4 E-05)nd12.2 ± 0.5
(P = 2.7 E-13)29 ± 0.3
(P = 6.2 E-12)32.4 ± 0.9
(P = 1.8 E-07)0.2 ± 0.040.3 ± 0.070.5 ± 0.020.4 ± 0.03
Wild type (R)22.8 ± 0.50.3 ± 0.02nd1 ± 0.072.6 ± 0.239.2 ± 1.328.5 ± 1.6nd3.3 ± 0.32.5 ± 0.2nd
D1o3-3 (R)15.7 ± 0.3
(P = 2.0 E-11)0.4 ± 0.1
(ns)nd2.8 ± 0.1 (P = 9.1 E-11)12.8 ± 0.6
(P = 6 E-11)45.9 ± 1
(P = 1.5 E-06)17.8 ± 0.7
(P = 3.2 E-08)nd2.5 ± 0.1
(P = 9.8 E-05)1.6 ± 0.1
(P = 8.5 E-07)0.5 ± 0.1
Wild type (L-TAG)37.0 ± 3.4ndndnd8.8 ± 2.223.7 ± 5.130.5 ± 1.4ndndndnd
D1o3-3 (L-TAG)12.5 ± 0.05
(P = 7.5 E-06)0.9 ± 0.011.6 ± 0.031.6 ± 0.0114.5 ± 0.01
(P = 0.0022)35.6 ± 0.2
(P = 0.0035)29.7 ± 0.09
(P = 0.2936)0.7 ± 0.070.8 ± 0.020.8 ± 0.11.2 ± 0.08
Wild type (R-TAG)ndndndndndndndndndndnd
D1o3-3 (R-TAG)15.1 ± 0.10.7 ± 0.02nd5.7 ± 0.221.6 ± 0.237.1 ± 0.414.1 ± 0.20.3 ± 0.033.1 ± 0.11.4 ± 0.10.8 ± 0.02
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