| Constructa . | Generationb . | Basisc . | NRLd . | Sharede . | Pairedf . | Diffractg . | Terminih . |
|---|---|---|---|---|---|---|---|
| 165a | 1 | NCP | 165 | – | 20 | no | PstI |
| 167a | 1 | NCP | 167 | – | 22 | no | PstI |
| 169a | 1 | NCP | 169 | – | 24 | yes | PstI |
| 169ak | 1 | NCP | 169 | – | 24 | yes | KpnI |
| 169an | 1 | NCP | 169 | 24 | yes | KpnI/SacI | |
| 171a | 1 | NCP | 171 | – | 26 | no | PstI |
| 173a | 1 | NCP | 173 | – | 28 | no | PstI |
| 175a | 1 | NCP | 175 | – | 30 | no | KpnI |
| 177a | 1 | NCP | 177 | – | 32 | no | PstI |
| 338b | 2 | 169a | 169 | 24 | 24 | yes | EcoRV |
| 343c | 3 | 338b | 171.5 | 29 | 24 | yes | KpnI |
| 345c | 3 | 338b | 172.5 | 29 | 26 | no | KpnI |
| 347c | 3 | 338b | 173.5 | 29 | 28 | no | KpnI |
| 349c | 3 | 338b | 174.5 | 29 | 30 | yesi | KpnI |
| 351c | 3 | 338b | 175.5 | 29 | 32 | no | KpnI |
| 353c | 3 | 338b | 176.5 | 29 | 34 | yes | KpnI |
| 357d | 4 | 349c | 178.5 | 29 | 38 | no | KpnI |
| 359d | 4 | 349c | 179.5 | 29 | 40 | no | KpnI |
| 361d | 4 | 349c | 180.5 | 29 | 42 | no | KpnI |
| 351e | 5 | 349c | 175.5 | 31 | 30 | no | KpnI |
| 353e | 5 | 351c | 176.5 | 31 | 32 | yes | KpnI |
| 355e | 5 | 353c | 177.5 | 31 | 34 | yesi | KpnI |
| 357e | 5 | 353c | 178.5 | 31 | 36 | no | KpnI |
| 354f | 6 | 353e | 177 | 32 | 32 | yes | KpnI |
| Constructa . | Generationb . | Basisc . | NRLd . | Sharede . | Pairedf . | Diffractg . | Terminih . |
|---|---|---|---|---|---|---|---|
| 165a | 1 | NCP | 165 | – | 20 | no | PstI |
| 167a | 1 | NCP | 167 | – | 22 | no | PstI |
| 169a | 1 | NCP | 169 | – | 24 | yes | PstI |
| 169ak | 1 | NCP | 169 | – | 24 | yes | KpnI |
| 169an | 1 | NCP | 169 | 24 | yes | KpnI/SacI | |
| 171a | 1 | NCP | 171 | – | 26 | no | PstI |
| 173a | 1 | NCP | 173 | – | 28 | no | PstI |
| 175a | 1 | NCP | 175 | – | 30 | no | KpnI |
| 177a | 1 | NCP | 177 | – | 32 | no | PstI |
| 338b | 2 | 169a | 169 | 24 | 24 | yes | EcoRV |
| 343c | 3 | 338b | 171.5 | 29 | 24 | yes | KpnI |
| 345c | 3 | 338b | 172.5 | 29 | 26 | no | KpnI |
| 347c | 3 | 338b | 173.5 | 29 | 28 | no | KpnI |
| 349c | 3 | 338b | 174.5 | 29 | 30 | yesi | KpnI |
| 351c | 3 | 338b | 175.5 | 29 | 32 | no | KpnI |
| 353c | 3 | 338b | 176.5 | 29 | 34 | yes | KpnI |
| 357d | 4 | 349c | 178.5 | 29 | 38 | no | KpnI |
| 359d | 4 | 349c | 179.5 | 29 | 40 | no | KpnI |
| 361d | 4 | 349c | 180.5 | 29 | 42 | no | KpnI |
| 351e | 5 | 349c | 175.5 | 31 | 30 | no | KpnI |
| 353e | 5 | 351c | 176.5 | 31 | 32 | yes | KpnI |
| 355e | 5 | 353c | 177.5 | 31 | 34 | yesi | KpnI |
| 357e | 5 | 353c | 178.5 | 31 | 36 | no | KpnI |
| 354f | 6 | 353e | 177 | 32 | 32 | yes | KpnI |
aConstruct identity.
bGeneration number in design process.
cBasis upon which construct was designed.
dNucleosome repeat length (bp).
eLength of the connecting linker DNA (bp) in the dinucleosome constructs.
fLength of the linker DNA (bp) formed by annealing of the termini.
gX-ray data set obtained to a resolution of at least 5.5 Å.
hRestriction enzyme(s) used to generate termini (4-nucleotide overhangs except for EcoRV, which generates blunt ends).
iFrom Adhireksan et al. (27).
| Constructa . | Generationb . | Basisc . | NRLd . | Sharede . | Pairedf . | Diffractg . | Terminih . |
|---|---|---|---|---|---|---|---|
| 165a | 1 | NCP | 165 | – | 20 | no | PstI |
| 167a | 1 | NCP | 167 | – | 22 | no | PstI |
| 169a | 1 | NCP | 169 | – | 24 | yes | PstI |
| 169ak | 1 | NCP | 169 | – | 24 | yes | KpnI |
| 169an | 1 | NCP | 169 | 24 | yes | KpnI/SacI | |
| 171a | 1 | NCP | 171 | – | 26 | no | PstI |
| 173a | 1 | NCP | 173 | – | 28 | no | PstI |
| 175a | 1 | NCP | 175 | – | 30 | no | KpnI |
| 177a | 1 | NCP | 177 | – | 32 | no | PstI |
| 338b | 2 | 169a | 169 | 24 | 24 | yes | EcoRV |
| 343c | 3 | 338b | 171.5 | 29 | 24 | yes | KpnI |
| 345c | 3 | 338b | 172.5 | 29 | 26 | no | KpnI |
| 347c | 3 | 338b | 173.5 | 29 | 28 | no | KpnI |
| 349c | 3 | 338b | 174.5 | 29 | 30 | yesi | KpnI |
| 351c | 3 | 338b | 175.5 | 29 | 32 | no | KpnI |
| 353c | 3 | 338b | 176.5 | 29 | 34 | yes | KpnI |
| 357d | 4 | 349c | 178.5 | 29 | 38 | no | KpnI |
| 359d | 4 | 349c | 179.5 | 29 | 40 | no | KpnI |
| 361d | 4 | 349c | 180.5 | 29 | 42 | no | KpnI |
| 351e | 5 | 349c | 175.5 | 31 | 30 | no | KpnI |
| 353e | 5 | 351c | 176.5 | 31 | 32 | yes | KpnI |
| 355e | 5 | 353c | 177.5 | 31 | 34 | yesi | KpnI |
| 357e | 5 | 353c | 178.5 | 31 | 36 | no | KpnI |
| 354f | 6 | 353e | 177 | 32 | 32 | yes | KpnI |
| Constructa . | Generationb . | Basisc . | NRLd . | Sharede . | Pairedf . | Diffractg . | Terminih . |
|---|---|---|---|---|---|---|---|
| 165a | 1 | NCP | 165 | – | 20 | no | PstI |
| 167a | 1 | NCP | 167 | – | 22 | no | PstI |
| 169a | 1 | NCP | 169 | – | 24 | yes | PstI |
| 169ak | 1 | NCP | 169 | – | 24 | yes | KpnI |
| 169an | 1 | NCP | 169 | 24 | yes | KpnI/SacI | |
| 171a | 1 | NCP | 171 | – | 26 | no | PstI |
| 173a | 1 | NCP | 173 | – | 28 | no | PstI |
| 175a | 1 | NCP | 175 | – | 30 | no | KpnI |
| 177a | 1 | NCP | 177 | – | 32 | no | PstI |
| 338b | 2 | 169a | 169 | 24 | 24 | yes | EcoRV |
| 343c | 3 | 338b | 171.5 | 29 | 24 | yes | KpnI |
| 345c | 3 | 338b | 172.5 | 29 | 26 | no | KpnI |
| 347c | 3 | 338b | 173.5 | 29 | 28 | no | KpnI |
| 349c | 3 | 338b | 174.5 | 29 | 30 | yesi | KpnI |
| 351c | 3 | 338b | 175.5 | 29 | 32 | no | KpnI |
| 353c | 3 | 338b | 176.5 | 29 | 34 | yes | KpnI |
| 357d | 4 | 349c | 178.5 | 29 | 38 | no | KpnI |
| 359d | 4 | 349c | 179.5 | 29 | 40 | no | KpnI |
| 361d | 4 | 349c | 180.5 | 29 | 42 | no | KpnI |
| 351e | 5 | 349c | 175.5 | 31 | 30 | no | KpnI |
| 353e | 5 | 351c | 176.5 | 31 | 32 | yes | KpnI |
| 355e | 5 | 353c | 177.5 | 31 | 34 | yesi | KpnI |
| 357e | 5 | 353c | 178.5 | 31 | 36 | no | KpnI |
| 354f | 6 | 353e | 177 | 32 | 32 | yes | KpnI |
aConstruct identity.
bGeneration number in design process.
cBasis upon which construct was designed.
dNucleosome repeat length (bp).
eLength of the connecting linker DNA (bp) in the dinucleosome constructs.
fLength of the linker DNA (bp) formed by annealing of the termini.
gX-ray data set obtained to a resolution of at least 5.5 Å.
hRestriction enzyme(s) used to generate termini (4-nucleotide overhangs except for EcoRV, which generates blunt ends).
iFrom Adhireksan et al. (27).
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