Comparison of |$\overline{T_{\rm p}}$| and |$\overline{T_{\rm f}}$| with melting temperatures (Tm) and unfolding forces determined in identical buffer conditions (10 mM Tris–HCl pH 7.5, 100 mM KCl), ranked by decreasing Tm)
| G4 . | |$\overline{T_{\rm p}}$| ± error . | |$\overline{T_{\rm f}}$| ± error . | Tp ≤ Tc . | Tm (°C) . | Unfolding force (pN) . |
|---|---|---|---|---|---|
| βA ori | 9000±1300 | 30±13 | 15|$\%$| | 80 (10) | nd |
| cMYC | 11 000±1700 | 90±10 | 10|$\%$| | 78 (69) | 22, 50 (42) |
| cKIT-2 | 5000±900 | 12 000±1700 | 30% | 70 (70) | nd |
| 21-TTA | 600±80 | 350±30 | 82% | 68 (68) | 11, 22 29, 36 (43) |
| 21-CTA | 350±60 | 1700±130 | 88% | 67 (70) | nd |
| 45-TTA | 500±5 | 150±13 | 83% | 59 (70) | nd |
| βA-m12 | 6500±1300 | 1400±170 | 15% | 55 (10) | nd |
| βA-m16 | 125±20 | 9000±1300 | 69% | 45 (10) | nd |
| G4 . | |$\overline{T_{\rm p}}$| ± error . | |$\overline{T_{\rm f}}$| ± error . | Tp ≤ Tc . | Tm (°C) . | Unfolding force (pN) . |
|---|---|---|---|---|---|
| βA ori | 9000±1300 | 30±13 | 15|$\%$| | 80 (10) | nd |
| cMYC | 11 000±1700 | 90±10 | 10|$\%$| | 78 (69) | 22, 50 (42) |
| cKIT-2 | 5000±900 | 12 000±1700 | 30% | 70 (70) | nd |
| 21-TTA | 600±80 | 350±30 | 82% | 68 (68) | 11, 22 29, 36 (43) |
| 21-CTA | 350±60 | 1700±130 | 88% | 67 (70) | nd |
| 45-TTA | 500±5 | 150±13 | 83% | 59 (70) | nd |
| βA-m12 | 6500±1300 | 1400±170 | 15% | 55 (10) | nd |
| βA-m16 | 125±20 | 9000±1300 | 69% | 45 (10) | nd |
nd: not determined. Tc: cycle time. references in parentheses.
Comparison of |$\overline{T_{\rm p}}$| and |$\overline{T_{\rm f}}$| with melting temperatures (Tm) and unfolding forces determined in identical buffer conditions (10 mM Tris–HCl pH 7.5, 100 mM KCl), ranked by decreasing Tm)
| G4 . | |$\overline{T_{\rm p}}$| ± error . | |$\overline{T_{\rm f}}$| ± error . | Tp ≤ Tc . | Tm (°C) . | Unfolding force (pN) . |
|---|---|---|---|---|---|
| βA ori | 9000±1300 | 30±13 | 15|$\%$| | 80 (10) | nd |
| cMYC | 11 000±1700 | 90±10 | 10|$\%$| | 78 (69) | 22, 50 (42) |
| cKIT-2 | 5000±900 | 12 000±1700 | 30% | 70 (70) | nd |
| 21-TTA | 600±80 | 350±30 | 82% | 68 (68) | 11, 22 29, 36 (43) |
| 21-CTA | 350±60 | 1700±130 | 88% | 67 (70) | nd |
| 45-TTA | 500±5 | 150±13 | 83% | 59 (70) | nd |
| βA-m12 | 6500±1300 | 1400±170 | 15% | 55 (10) | nd |
| βA-m16 | 125±20 | 9000±1300 | 69% | 45 (10) | nd |
| G4 . | |$\overline{T_{\rm p}}$| ± error . | |$\overline{T_{\rm f}}$| ± error . | Tp ≤ Tc . | Tm (°C) . | Unfolding force (pN) . |
|---|---|---|---|---|---|
| βA ori | 9000±1300 | 30±13 | 15|$\%$| | 80 (10) | nd |
| cMYC | 11 000±1700 | 90±10 | 10|$\%$| | 78 (69) | 22, 50 (42) |
| cKIT-2 | 5000±900 | 12 000±1700 | 30% | 70 (70) | nd |
| 21-TTA | 600±80 | 350±30 | 82% | 68 (68) | 11, 22 29, 36 (43) |
| 21-CTA | 350±60 | 1700±130 | 88% | 67 (70) | nd |
| 45-TTA | 500±5 | 150±13 | 83% | 59 (70) | nd |
| βA-m12 | 6500±1300 | 1400±170 | 15% | 55 (10) | nd |
| βA-m16 | 125±20 | 9000±1300 | 69% | 45 (10) | nd |
nd: not determined. Tc: cycle time. references in parentheses.
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