| Mutant Strains . | Gene . | Mice Survival During Pneumonia . | Biofilm Formation . | Attachment . | Fitness . | Fitness in Iron-Limiting Conditions . | Increase Antibiotic Susceptibility . |
|---|---|---|---|---|---|---|---|
| Virulence loss in pneumonia | |||||||
| Δ0242 | feoA | ++ | ++ | +++ | − | ++ | − |
| Δ0781 | mtnN | + | + | − | − | − | − |
| Δ1657 | bnfL, | + | ++ | +++ | − | + | − |
| Δ2247 | yfgC | + | − | − | + | − | + |
| Δ2390 | basB | + | − | +++ | − | +++ | − |
| Δ3245 | hisF | +++ | − | − | − | − | − |
| Δ3410 | oatA | + | − | − | − | − | − |
| No virulence loss in pneumonia | |||||||
| Δ0094 | lrp | − | − | − | − | − | − |
| Δ0315 | Oxygenase-like | − | − | ++ | − | − | − |
| Δ0318 | fusE-MFP/hylD | − | − | − | − | − | − |
| Δ0896 | pilU | − | − | − | − | − | − |
| Δ1028 | ligB | − | − | − | − | − | − |
| Δ3032 | tonB-like | − | − | ++ | − | − | − |
| Δ3290 | esvF2 | − | +++ a | ++ | − | − | − |
| Mutant Strains . | Gene . | Mice Survival During Pneumonia . | Biofilm Formation . | Attachment . | Fitness . | Fitness in Iron-Limiting Conditions . | Increase Antibiotic Susceptibility . |
|---|---|---|---|---|---|---|---|
| Virulence loss in pneumonia | |||||||
| Δ0242 | feoA | ++ | ++ | +++ | − | ++ | − |
| Δ0781 | mtnN | + | + | − | − | − | − |
| Δ1657 | bnfL, | + | ++ | +++ | − | + | − |
| Δ2247 | yfgC | + | − | − | + | − | + |
| Δ2390 | basB | + | − | +++ | − | +++ | − |
| Δ3245 | hisF | +++ | − | − | − | − | − |
| Δ3410 | oatA | + | − | − | − | − | − |
| No virulence loss in pneumonia | |||||||
| Δ0094 | lrp | − | − | − | − | − | − |
| Δ0315 | Oxygenase-like | − | − | ++ | − | − | − |
| Δ0318 | fusE-MFP/hylD | − | − | − | − | − | − |
| Δ0896 | pilU | − | − | − | − | − | − |
| Δ1028 | ligB | − | − | − | − | − | − |
| Δ3032 | tonB-like | − | − | ++ | − | − | − |
| Δ3290 | esvF2 | − | +++ a | ++ | − | − | − |
Differences in bacterial phenotypes observed in the mutant strains compared with the parental ATCC 17978 strain are indicated as follows: +, 20%–49%; ++, 50%–74%; +++, 75%–100%; −, no difference.
Statistical analyses were performed using unpaired student t tests comparing each mutant with the ATCC 17978 wild type strain. Only differences statistically significant (P < .05) are shown.
aMutants improved the phenotype displayed by the parental strain.
| Mutant Strains . | Gene . | Mice Survival During Pneumonia . | Biofilm Formation . | Attachment . | Fitness . | Fitness in Iron-Limiting Conditions . | Increase Antibiotic Susceptibility . |
|---|---|---|---|---|---|---|---|
| Virulence loss in pneumonia | |||||||
| Δ0242 | feoA | ++ | ++ | +++ | − | ++ | − |
| Δ0781 | mtnN | + | + | − | − | − | − |
| Δ1657 | bnfL, | + | ++ | +++ | − | + | − |
| Δ2247 | yfgC | + | − | − | + | − | + |
| Δ2390 | basB | + | − | +++ | − | +++ | − |
| Δ3245 | hisF | +++ | − | − | − | − | − |
| Δ3410 | oatA | + | − | − | − | − | − |
| No virulence loss in pneumonia | |||||||
| Δ0094 | lrp | − | − | − | − | − | − |
| Δ0315 | Oxygenase-like | − | − | ++ | − | − | − |
| Δ0318 | fusE-MFP/hylD | − | − | − | − | − | − |
| Δ0896 | pilU | − | − | − | − | − | − |
| Δ1028 | ligB | − | − | − | − | − | − |
| Δ3032 | tonB-like | − | − | ++ | − | − | − |
| Δ3290 | esvF2 | − | +++ a | ++ | − | − | − |
| Mutant Strains . | Gene . | Mice Survival During Pneumonia . | Biofilm Formation . | Attachment . | Fitness . | Fitness in Iron-Limiting Conditions . | Increase Antibiotic Susceptibility . |
|---|---|---|---|---|---|---|---|
| Virulence loss in pneumonia | |||||||
| Δ0242 | feoA | ++ | ++ | +++ | − | ++ | − |
| Δ0781 | mtnN | + | + | − | − | − | − |
| Δ1657 | bnfL, | + | ++ | +++ | − | + | − |
| Δ2247 | yfgC | + | − | − | + | − | + |
| Δ2390 | basB | + | − | +++ | − | +++ | − |
| Δ3245 | hisF | +++ | − | − | − | − | − |
| Δ3410 | oatA | + | − | − | − | − | − |
| No virulence loss in pneumonia | |||||||
| Δ0094 | lrp | − | − | − | − | − | − |
| Δ0315 | Oxygenase-like | − | − | ++ | − | − | − |
| Δ0318 | fusE-MFP/hylD | − | − | − | − | − | − |
| Δ0896 | pilU | − | − | − | − | − | − |
| Δ1028 | ligB | − | − | − | − | − | − |
| Δ3032 | tonB-like | − | − | ++ | − | − | − |
| Δ3290 | esvF2 | − | +++ a | ++ | − | − | − |
Differences in bacterial phenotypes observed in the mutant strains compared with the parental ATCC 17978 strain are indicated as follows: +, 20%–49%; ++, 50%–74%; +++, 75%–100%; −, no difference.
Statistical analyses were performed using unpaired student t tests comparing each mutant with the ATCC 17978 wild type strain. Only differences statistically significant (P < .05) are shown.
aMutants improved the phenotype displayed by the parental strain.
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