The stability of the expression of these five genes was tested across 30 independent conditions, including different tissues, developmental stages, varieties, and disease infection (for details, see Supplemental Table S7).
| Ensembl Transcript Identifier . | Primer Sequences (5′–3′) . | Primer Efficiency (%) . | Coefficient of Variation (%) . |
|---|---|---|---|
| Traes_4AL_8CEA69D2E.1 | CGGGCCCGAAGAGAGTCT | 97.1 | 7.1 |
| ATTAACGAAACCAATCGACGGA | |||
| Traes_4BS_4AD56C4F8.2 | TCGTTGCTTGAGGAAAATG | 93.7 | 8.2 |
| CATGACCGTCTTATTTATGGCA | |||
| Traes_1AL_968B97E50.1 | TTTGCACAGTATGTACCAAATGAG | 95.0 | 5.8 |
| TCTTCCAATCAAAACCTCCTCT | |||
| Traes_5AS_019ECA143.1 | TCTAAATGTCCAGGAAGCTGTTA | 96.0 | 4.4 |
| CCTGTGGTGCCCAACTATT | |||
| Traes_6DS_BE8B5E56D.1 | CATGCTCTGGGATTTATCCAT | 93.3 | 8.4 |
| CTGGATCATTTCCGGTGC |
| Ensembl Transcript Identifier . | Primer Sequences (5′–3′) . | Primer Efficiency (%) . | Coefficient of Variation (%) . |
|---|---|---|---|
| Traes_4AL_8CEA69D2E.1 | CGGGCCCGAAGAGAGTCT | 97.1 | 7.1 |
| ATTAACGAAACCAATCGACGGA | |||
| Traes_4BS_4AD56C4F8.2 | TCGTTGCTTGAGGAAAATG | 93.7 | 8.2 |
| CATGACCGTCTTATTTATGGCA | |||
| Traes_1AL_968B97E50.1 | TTTGCACAGTATGTACCAAATGAG | 95.0 | 5.8 |
| TCTTCCAATCAAAACCTCCTCT | |||
| Traes_5AS_019ECA143.1 | TCTAAATGTCCAGGAAGCTGTTA | 96.0 | 4.4 |
| CCTGTGGTGCCCAACTATT | |||
| Traes_6DS_BE8B5E56D.1 | CATGCTCTGGGATTTATCCAT | 93.3 | 8.4 |
| CTGGATCATTTCCGGTGC |
The stability of the expression of these five genes was tested across 30 independent conditions, including different tissues, developmental stages, varieties, and disease infection (for details, see Supplemental Table S7).
| Ensembl Transcript Identifier . | Primer Sequences (5′–3′) . | Primer Efficiency (%) . | Coefficient of Variation (%) . |
|---|---|---|---|
| Traes_4AL_8CEA69D2E.1 | CGGGCCCGAAGAGAGTCT | 97.1 | 7.1 |
| ATTAACGAAACCAATCGACGGA | |||
| Traes_4BS_4AD56C4F8.2 | TCGTTGCTTGAGGAAAATG | 93.7 | 8.2 |
| CATGACCGTCTTATTTATGGCA | |||
| Traes_1AL_968B97E50.1 | TTTGCACAGTATGTACCAAATGAG | 95.0 | 5.8 |
| TCTTCCAATCAAAACCTCCTCT | |||
| Traes_5AS_019ECA143.1 | TCTAAATGTCCAGGAAGCTGTTA | 96.0 | 4.4 |
| CCTGTGGTGCCCAACTATT | |||
| Traes_6DS_BE8B5E56D.1 | CATGCTCTGGGATTTATCCAT | 93.3 | 8.4 |
| CTGGATCATTTCCGGTGC |
| Ensembl Transcript Identifier . | Primer Sequences (5′–3′) . | Primer Efficiency (%) . | Coefficient of Variation (%) . |
|---|---|---|---|
| Traes_4AL_8CEA69D2E.1 | CGGGCCCGAAGAGAGTCT | 97.1 | 7.1 |
| ATTAACGAAACCAATCGACGGA | |||
| Traes_4BS_4AD56C4F8.2 | TCGTTGCTTGAGGAAAATG | 93.7 | 8.2 |
| CATGACCGTCTTATTTATGGCA | |||
| Traes_1AL_968B97E50.1 | TTTGCACAGTATGTACCAAATGAG | 95.0 | 5.8 |
| TCTTCCAATCAAAACCTCCTCT | |||
| Traes_5AS_019ECA143.1 | TCTAAATGTCCAGGAAGCTGTTA | 96.0 | 4.4 |
| CCTGTGGTGCCCAACTATT | |||
| Traes_6DS_BE8B5E56D.1 | CATGCTCTGGGATTTATCCAT | 93.3 | 8.4 |
| CTGGATCATTTCCGGTGC |
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