The oligonucleotides were used for cDNA cloning of HcCNL (primers 1–5) and RT-PCR analysis of transcript levels of CNL (primers 3 and 4), PAL (primers 6 and 7), and 18S rDNA (primers 8 and 9). Oligonucleotides 10, 11, and 12 were Gateway cloning primers.
| Primer . | Sequence . |
|---|---|
| 1 | 5′-ATGGATAAGCTCCCAAAATGTGGTGCC-3′ |
| 2 | 5′-TTAGCCATATCCCTCAACTGGGCC-3′ |
| 3 | 5′-TCAGCAGCGTGGAGGTCGAGTCG-3′ |
| 4 | 5′-TTAAAGACGAGACATGGCAAG-3′ |
| 5 | 5′-ATTGAATTCTTAAAGACGAGACATGGCAAG-3′a |
| 6 | 5′-CTGCATTCCTTGATCTTGTTC-3′ |
| 7 | 5′-ACATCGAGGAGAATCTGAAGAG-3′ |
| 8 | 5′-TGATGGTATCTACTACTCGG-3′ |
| 9 | 5′-AATATACGCTATTGGAGCTGG-3′ |
| 10 | 5′-GGGGACAAGTTTGTACAAAAAAGCAGGCTTAACCATGGATAAGCTCCCAAAATGTGG-3′ |
| 11 | 5′-GGGGACCACTTTGTACAAGAAAGCTGGGTCTTAAAGACGAGACATGGCAAGAAC-3′ |
| 12 | 5′-GGGGACCACTTTGTACAAGAAAGCTGGGTCAAGACGAGACATGGCAAGAACAGG-3′ |
| Primer . | Sequence . |
|---|---|
| 1 | 5′-ATGGATAAGCTCCCAAAATGTGGTGCC-3′ |
| 2 | 5′-TTAGCCATATCCCTCAACTGGGCC-3′ |
| 3 | 5′-TCAGCAGCGTGGAGGTCGAGTCG-3′ |
| 4 | 5′-TTAAAGACGAGACATGGCAAG-3′ |
| 5 | 5′-ATTGAATTCTTAAAGACGAGACATGGCAAG-3′a |
| 6 | 5′-CTGCATTCCTTGATCTTGTTC-3′ |
| 7 | 5′-ACATCGAGGAGAATCTGAAGAG-3′ |
| 8 | 5′-TGATGGTATCTACTACTCGG-3′ |
| 9 | 5′-AATATACGCTATTGGAGCTGG-3′ |
| 10 | 5′-GGGGACAAGTTTGTACAAAAAAGCAGGCTTAACCATGGATAAGCTCCCAAAATGTGG-3′ |
| 11 | 5′-GGGGACCACTTTGTACAAGAAAGCTGGGTCTTAAAGACGAGACATGGCAAGAAC-3′ |
| 12 | 5′-GGGGACCACTTTGTACAAGAAAGCTGGGTCAAGACGAGACATGGCAAGAACAGG-3′ |
EcoRI restriction site is underlined.
The oligonucleotides were used for cDNA cloning of HcCNL (primers 1–5) and RT-PCR analysis of transcript levels of CNL (primers 3 and 4), PAL (primers 6 and 7), and 18S rDNA (primers 8 and 9). Oligonucleotides 10, 11, and 12 were Gateway cloning primers.
| Primer . | Sequence . |
|---|---|
| 1 | 5′-ATGGATAAGCTCCCAAAATGTGGTGCC-3′ |
| 2 | 5′-TTAGCCATATCCCTCAACTGGGCC-3′ |
| 3 | 5′-TCAGCAGCGTGGAGGTCGAGTCG-3′ |
| 4 | 5′-TTAAAGACGAGACATGGCAAG-3′ |
| 5 | 5′-ATTGAATTCTTAAAGACGAGACATGGCAAG-3′a |
| 6 | 5′-CTGCATTCCTTGATCTTGTTC-3′ |
| 7 | 5′-ACATCGAGGAGAATCTGAAGAG-3′ |
| 8 | 5′-TGATGGTATCTACTACTCGG-3′ |
| 9 | 5′-AATATACGCTATTGGAGCTGG-3′ |
| 10 | 5′-GGGGACAAGTTTGTACAAAAAAGCAGGCTTAACCATGGATAAGCTCCCAAAATGTGG-3′ |
| 11 | 5′-GGGGACCACTTTGTACAAGAAAGCTGGGTCTTAAAGACGAGACATGGCAAGAAC-3′ |
| 12 | 5′-GGGGACCACTTTGTACAAGAAAGCTGGGTCAAGACGAGACATGGCAAGAACAGG-3′ |
| Primer . | Sequence . |
|---|---|
| 1 | 5′-ATGGATAAGCTCCCAAAATGTGGTGCC-3′ |
| 2 | 5′-TTAGCCATATCCCTCAACTGGGCC-3′ |
| 3 | 5′-TCAGCAGCGTGGAGGTCGAGTCG-3′ |
| 4 | 5′-TTAAAGACGAGACATGGCAAG-3′ |
| 5 | 5′-ATTGAATTCTTAAAGACGAGACATGGCAAG-3′a |
| 6 | 5′-CTGCATTCCTTGATCTTGTTC-3′ |
| 7 | 5′-ACATCGAGGAGAATCTGAAGAG-3′ |
| 8 | 5′-TGATGGTATCTACTACTCGG-3′ |
| 9 | 5′-AATATACGCTATTGGAGCTGG-3′ |
| 10 | 5′-GGGGACAAGTTTGTACAAAAAAGCAGGCTTAACCATGGATAAGCTCCCAAAATGTGG-3′ |
| 11 | 5′-GGGGACCACTTTGTACAAGAAAGCTGGGTCTTAAAGACGAGACATGGCAAGAAC-3′ |
| 12 | 5′-GGGGACCACTTTGTACAAGAAAGCTGGGTCAAGACGAGACATGGCAAGAACAGG-3′ |
EcoRI restriction site is underlined.
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