| Bait Protein . | Protein Identified . | Spectral Counts . | Unique Peptides . | Percentage Coverage . |
|---|---|---|---|---|
| C4H | CPR1 | 5 | 3 | 6.8 |
| CPR2 | 2 | 1 | 2.1 | |
| CBR1 | 7 | 4 | 18 | |
| CB5D | 7 (4) | 4 (3) | 41 (36) | |
| CB5C | 4 | 2 | 17 | |
| CB5E | 1 (2) | 1 (2) | 10 (28) | |
| C3′H | CPR1 | 4 | 3 | 7.4 |
| CPR2 | 1 | 1 | 2.3 | |
| CBR1 | 5 | 3 | 15 | |
| CB5D | 7 (1) | 4 (1) | 41 (9.3) | |
| CB5C | 2 | 1 | 7.6 | |
| CB5E | 3 | 2 | 16 | |
| F5H | CPR1 | 4 | 3 | 6.9 |
| CPR2 | 1 | 1 | 2.3 | |
| CBR1 | 5 | 4 | 18 | |
| CB5D | 4 (2) | 2 (2) | 25 (24) | |
| CB5C | 4 | 2 | 17 | |
| CB5E | 2 | 1 | 10 |
| Bait Protein . | Protein Identified . | Spectral Counts . | Unique Peptides . | Percentage Coverage . |
|---|---|---|---|---|
| C4H | CPR1 | 5 | 3 | 6.8 |
| CPR2 | 2 | 1 | 2.1 | |
| CBR1 | 7 | 4 | 18 | |
| CB5D | 7 (4) | 4 (3) | 41 (36) | |
| CB5C | 4 | 2 | 17 | |
| CB5E | 1 (2) | 1 (2) | 10 (28) | |
| C3′H | CPR1 | 4 | 3 | 7.4 |
| CPR2 | 1 | 1 | 2.3 | |
| CBR1 | 5 | 3 | 15 | |
| CB5D | 7 (1) | 4 (1) | 41 (9.3) | |
| CB5C | 2 | 1 | 7.6 | |
| CB5E | 3 | 2 | 16 | |
| F5H | CPR1 | 4 | 3 | 6.9 |
| CPR2 | 1 | 1 | 2.3 | |
| CBR1 | 5 | 4 | 18 | |
| CB5D | 4 (2) | 2 (2) | 25 (24) | |
| CB5C | 4 | 2 | 17 | |
| CB5E | 2 | 1 | 10 |
Purified proteins were subjected to SDS-PAGE, and the sliced gel pieces were processed for in-gel digestion and peptide elution (for data in parentheses), or the proteins were digested and eluted directly from protein-bound beads. The eluted peptides were injected for LC-MS analysis. The MS/MS data was searched with GPM X!Tandem software against the UniProt Arabidopsis database with P = 0.01.
| Bait Protein . | Protein Identified . | Spectral Counts . | Unique Peptides . | Percentage Coverage . |
|---|---|---|---|---|
| C4H | CPR1 | 5 | 3 | 6.8 |
| CPR2 | 2 | 1 | 2.1 | |
| CBR1 | 7 | 4 | 18 | |
| CB5D | 7 (4) | 4 (3) | 41 (36) | |
| CB5C | 4 | 2 | 17 | |
| CB5E | 1 (2) | 1 (2) | 10 (28) | |
| C3′H | CPR1 | 4 | 3 | 7.4 |
| CPR2 | 1 | 1 | 2.3 | |
| CBR1 | 5 | 3 | 15 | |
| CB5D | 7 (1) | 4 (1) | 41 (9.3) | |
| CB5C | 2 | 1 | 7.6 | |
| CB5E | 3 | 2 | 16 | |
| F5H | CPR1 | 4 | 3 | 6.9 |
| CPR2 | 1 | 1 | 2.3 | |
| CBR1 | 5 | 4 | 18 | |
| CB5D | 4 (2) | 2 (2) | 25 (24) | |
| CB5C | 4 | 2 | 17 | |
| CB5E | 2 | 1 | 10 |
| Bait Protein . | Protein Identified . | Spectral Counts . | Unique Peptides . | Percentage Coverage . |
|---|---|---|---|---|
| C4H | CPR1 | 5 | 3 | 6.8 |
| CPR2 | 2 | 1 | 2.1 | |
| CBR1 | 7 | 4 | 18 | |
| CB5D | 7 (4) | 4 (3) | 41 (36) | |
| CB5C | 4 | 2 | 17 | |
| CB5E | 1 (2) | 1 (2) | 10 (28) | |
| C3′H | CPR1 | 4 | 3 | 7.4 |
| CPR2 | 1 | 1 | 2.3 | |
| CBR1 | 5 | 3 | 15 | |
| CB5D | 7 (1) | 4 (1) | 41 (9.3) | |
| CB5C | 2 | 1 | 7.6 | |
| CB5E | 3 | 2 | 16 | |
| F5H | CPR1 | 4 | 3 | 6.9 |
| CPR2 | 1 | 1 | 2.3 | |
| CBR1 | 5 | 4 | 18 | |
| CB5D | 4 (2) | 2 (2) | 25 (24) | |
| CB5C | 4 | 2 | 17 | |
| CB5E | 2 | 1 | 10 |
Purified proteins were subjected to SDS-PAGE, and the sliced gel pieces were processed for in-gel digestion and peptide elution (for data in parentheses), or the proteins were digested and eluted directly from protein-bound beads. The eluted peptides were injected for LC-MS analysis. The MS/MS data was searched with GPM X!Tandem software against the UniProt Arabidopsis database with P = 0.01.
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