During the 2008 vintage, four pilot fermentations in 30,000-l vats were conducted with J4 and J5 candidates as starter strains. Kinetics fermentation behavior was checked by four daily temperature and sugar content measurements all through the scaling phases. Average sugar content consumption slopes for the two strains in the last scale phase (25,000 l) is represented in Fig. 3. Fermentation kinetics for J4 and J5 were better than that obtained for commercial C2 reference strain. Other interesting oenological parameters were determined at the end of fermentations. Since J4 and J5 strains were previously selected from spontaneous fermentations, data of a spontaneous fermentation are shown as well (Table 3). The J4 and J5 strains obtained similar values for oenological parameters. The J4 strain reached the highest alcoholic grade at the end of fermentation.
Sugar consumption (ºBé) in pilot-scale fermentations. Results are average, and standard deviations of four fermentations conducted by J4 strain (filled circles) and four fermentations conducted by J5 strain (empty circles), compared with one representative fermentation conducted by C2 reference strain (triangles)
Fig. 3

Sugar consumption (ºBé) in pilot-scale fermentations. Results are average, and standard deviations of four fermentations conducted by J4 strain (filled circles) and four fermentations conducted by J5 strain (empty circles), compared with one representative fermentation conducted by C2 reference strain (triangles)

 

Final parameters for J4, J5, and reference strains measured at the end of non-sterile pilot-scale fermentations

StrainEthanol content (%)Volatile acidity (g/l)Sugars content (g/l)pHTotal acidity (g/l)
J413.26 ± 0.150.29 ± 0.010.28 ± 0.063.15 ± 0.015.68 ± 0.07
J512.81 ± 0.040.30 ± 0.010.28 ± 0.043.16 ± 0.015.69 ± 0.04
Sa12.790.320.453.185.47
C212.960.312.543.227.36
StrainEthanol content (%)Volatile acidity (g/l)Sugars content (g/l)pHTotal acidity (g/l)
J413.26 ± 0.150.29 ± 0.010.28 ± 0.063.15 ± 0.015.68 ± 0.07
J512.81 ± 0.040.30 ± 0.010.28 ± 0.043.16 ± 0.015.69 ± 0.04
Sa12.790.320.453.185.47
C212.960.312.543.227.36

aS indicates spontaneous fermentation

J4 and J5 results are average, and standard deviation of four pilot fermentations in duplicate

S and C2 are representative fermentations

Final parameters for J4, J5, and reference strains measured at the end of non-sterile pilot-scale fermentations

StrainEthanol content (%)Volatile acidity (g/l)Sugars content (g/l)pHTotal acidity (g/l)
J413.26 ± 0.150.29 ± 0.010.28 ± 0.063.15 ± 0.015.68 ± 0.07
J512.81 ± 0.040.30 ± 0.010.28 ± 0.043.16 ± 0.015.69 ± 0.04
Sa12.790.320.453.185.47
C212.960.312.543.227.36
StrainEthanol content (%)Volatile acidity (g/l)Sugars content (g/l)pHTotal acidity (g/l)
J413.26 ± 0.150.29 ± 0.010.28 ± 0.063.15 ± 0.015.68 ± 0.07
J512.81 ± 0.040.30 ± 0.010.28 ± 0.043.16 ± 0.015.69 ± 0.04
Sa12.790.320.453.185.47
C212.960.312.543.227.36

aS indicates spontaneous fermentation

J4 and J5 results are average, and standard deviation of four pilot fermentations in duplicate

S and C2 are representative fermentations

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