Table 2.
Transmission Rates of Mutant and Complemented zix Alleles
GenotypeKan  r/Kan  sn
Mutant alleles
zix-1/ZIX selfed(229/166) 1.38395
zix-2/ZIX selfed(483/273) 1.77756
zix-3/ZIX selfed(588/353) 1.66941
zix-1/ZIX × ZIX/ZIX(94/251) 0.38345
ZIX/ZIX × zix-1/ZIX(193/278) 0.7471
zix-2/ZIX × ZIX/ZIX(98/242) 0.37340
ZIX/ZIX × zix-2/ZIX(308/518) 0.6826
zix-3/ZIX × ZIX/ZIX(85/233) 0.37317
ZIX/ZIX × zix-3/ZIX(276/343) 0.8619
Complemented alleles with genomic ZIX
zix-1/ZIX  ;Lg/- (1) selfed(540/212) 2.55754
zix-1/ZIX  ;Lg/- (2) selfed(603/227) 2.66830
zix-1/ZIX  ;Lg/- (3) selfed(954/280) 3.411234
zix-2/ZIX  ;Lg/- (1) selfed(931/354) 2.631285
zix-2/ZIX  ;Lg/- (2) selfed(897/383) 2.341280
zix-2/ZIX  ;Lg/- (3) selfed(949/390) 2.431289
zix-3/ZIX  ;Lg/- (1) selfed(935/195) 4.81130
zix-3/ZIX  ;Lg/- (2) selfed(648/254) 2.55902
zix-3/ZIX  ;Lg/- (3) selfed(798/196) 4.7994
Complemented alleles with ProZIX:ZIX-GFP
zix-3/ZIX  ;ZG/- (1) selfed(345/147) 2.35492
zix-3/ZIX  ;ZG/- (2) selfed(195/104) 1.88299
zix-3/ZIX  ;ZG/- (3) selfed(179/81) 2.2260
zix-3/ZIX  ;ZG/- (4) selfed(279/128) 2.18407
zix-3/ZIX  ;ZG/- (5) selfed(228/81) 2.8309
GenotypeKan  r/Kan  sn
Mutant alleles
zix-1/ZIX selfed(229/166) 1.38395
zix-2/ZIX selfed(483/273) 1.77756
zix-3/ZIX selfed(588/353) 1.66941
zix-1/ZIX × ZIX/ZIX(94/251) 0.38345
ZIX/ZIX × zix-1/ZIX(193/278) 0.7471
zix-2/ZIX × ZIX/ZIX(98/242) 0.37340
ZIX/ZIX × zix-2/ZIX(308/518) 0.6826
zix-3/ZIX × ZIX/ZIX(85/233) 0.37317
ZIX/ZIX × zix-3/ZIX(276/343) 0.8619
Complemented alleles with genomic ZIX
zix-1/ZIX  ;Lg/- (1) selfed(540/212) 2.55754
zix-1/ZIX  ;Lg/- (2) selfed(603/227) 2.66830
zix-1/ZIX  ;Lg/- (3) selfed(954/280) 3.411234
zix-2/ZIX  ;Lg/- (1) selfed(931/354) 2.631285
zix-2/ZIX  ;Lg/- (2) selfed(897/383) 2.341280
zix-2/ZIX  ;Lg/- (3) selfed(949/390) 2.431289
zix-3/ZIX  ;Lg/- (1) selfed(935/195) 4.81130
zix-3/ZIX  ;Lg/- (2) selfed(648/254) 2.55902
zix-3/ZIX  ;Lg/- (3) selfed(798/196) 4.7994
Complemented alleles with ProZIX:ZIX-GFP
zix-3/ZIX  ;ZG/- (1) selfed(345/147) 2.35492
zix-3/ZIX  ;ZG/- (2) selfed(195/104) 1.88299
zix-3/ZIX  ;ZG/- (3) selfed(179/81) 2.2260
zix-3/ZIX  ;ZG/- (4) selfed(279/128) 2.18407
zix-3/ZIX  ;ZG/- (5) selfed(228/81) 2.8309

Kan-resistant and -sensitive plants were scored on MS plates containing 50 mg/L Kan at 2 weeks after seed germination. Raw numbers in each class are given in parentheses. Lg, genomic At4g00231 transgene; ZG, ZIX:ZIX-GFP transgene; n, numbers of plants.

Table 2.
Transmission Rates of Mutant and Complemented zix Alleles
GenotypeKan  r/Kan  sn
Mutant alleles
zix-1/ZIX selfed(229/166) 1.38395
zix-2/ZIX selfed(483/273) 1.77756
zix-3/ZIX selfed(588/353) 1.66941
zix-1/ZIX × ZIX/ZIX(94/251) 0.38345
ZIX/ZIX × zix-1/ZIX(193/278) 0.7471
zix-2/ZIX × ZIX/ZIX(98/242) 0.37340
ZIX/ZIX × zix-2/ZIX(308/518) 0.6826
zix-3/ZIX × ZIX/ZIX(85/233) 0.37317
ZIX/ZIX × zix-3/ZIX(276/343) 0.8619
Complemented alleles with genomic ZIX
zix-1/ZIX  ;Lg/- (1) selfed(540/212) 2.55754
zix-1/ZIX  ;Lg/- (2) selfed(603/227) 2.66830
zix-1/ZIX  ;Lg/- (3) selfed(954/280) 3.411234
zix-2/ZIX  ;Lg/- (1) selfed(931/354) 2.631285
zix-2/ZIX  ;Lg/- (2) selfed(897/383) 2.341280
zix-2/ZIX  ;Lg/- (3) selfed(949/390) 2.431289
zix-3/ZIX  ;Lg/- (1) selfed(935/195) 4.81130
zix-3/ZIX  ;Lg/- (2) selfed(648/254) 2.55902
zix-3/ZIX  ;Lg/- (3) selfed(798/196) 4.7994
Complemented alleles with ProZIX:ZIX-GFP
zix-3/ZIX  ;ZG/- (1) selfed(345/147) 2.35492
zix-3/ZIX  ;ZG/- (2) selfed(195/104) 1.88299
zix-3/ZIX  ;ZG/- (3) selfed(179/81) 2.2260
zix-3/ZIX  ;ZG/- (4) selfed(279/128) 2.18407
zix-3/ZIX  ;ZG/- (5) selfed(228/81) 2.8309
GenotypeKan  r/Kan  sn
Mutant alleles
zix-1/ZIX selfed(229/166) 1.38395
zix-2/ZIX selfed(483/273) 1.77756
zix-3/ZIX selfed(588/353) 1.66941
zix-1/ZIX × ZIX/ZIX(94/251) 0.38345
ZIX/ZIX × zix-1/ZIX(193/278) 0.7471
zix-2/ZIX × ZIX/ZIX(98/242) 0.37340
ZIX/ZIX × zix-2/ZIX(308/518) 0.6826
zix-3/ZIX × ZIX/ZIX(85/233) 0.37317
ZIX/ZIX × zix-3/ZIX(276/343) 0.8619
Complemented alleles with genomic ZIX
zix-1/ZIX  ;Lg/- (1) selfed(540/212) 2.55754
zix-1/ZIX  ;Lg/- (2) selfed(603/227) 2.66830
zix-1/ZIX  ;Lg/- (3) selfed(954/280) 3.411234
zix-2/ZIX  ;Lg/- (1) selfed(931/354) 2.631285
zix-2/ZIX  ;Lg/- (2) selfed(897/383) 2.341280
zix-2/ZIX  ;Lg/- (3) selfed(949/390) 2.431289
zix-3/ZIX  ;Lg/- (1) selfed(935/195) 4.81130
zix-3/ZIX  ;Lg/- (2) selfed(648/254) 2.55902
zix-3/ZIX  ;Lg/- (3) selfed(798/196) 4.7994
Complemented alleles with ProZIX:ZIX-GFP
zix-3/ZIX  ;ZG/- (1) selfed(345/147) 2.35492
zix-3/ZIX  ;ZG/- (2) selfed(195/104) 1.88299
zix-3/ZIX  ;ZG/- (3) selfed(179/81) 2.2260
zix-3/ZIX  ;ZG/- (4) selfed(279/128) 2.18407
zix-3/ZIX  ;ZG/- (5) selfed(228/81) 2.8309

Kan-resistant and -sensitive plants were scored on MS plates containing 50 mg/L Kan at 2 weeks after seed germination. Raw numbers in each class are given in parentheses. Lg, genomic At4g00231 transgene; ZG, ZIX:ZIX-GFP transgene; n, numbers of plants.

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