| Gene . | Primer sequence . |
|---|---|
| Snai1 | Forward: 5ʹ-TTACCTTCCAGCAGCCCTAC-3ʹ |
| Reverse: 5ʹ-AGCCTTTCCCACTGTCCTC-3ʹ | |
| Twist | Forward: 5ʹ-CGACGACAGCCTGAGCAACA-3ʹ |
| Reverse: 5ʹ-CCACAGCCCGCAGACTTCTT-3ʹ | |
| Slug | Forward: 5ʹ-CCTCCATCTGACACCTCC-3ʹ |
| Reverse: 5ʹ-CCCAGGCTCACATATTCC-3ʹ | |
| Zeb1 | Forward: 5ʹ-AAGTGGCGGTAGATGGTA-3ʹ |
| Reverse: 5ʹ-TTGTAGCGACTGGATTTT-3ʹ | |
| Vimentin | Forward: 5ʹ-TTGAACGCAAAGTGGAATC-3ʹ |
| Reverse: 5ʹ-AGGTCAGGCTTGGAAACA-3ʹ | |
| E-cadherin | Forward: 5ʹ-CTGAGAACGAGGCTAACG-3ʹ |
| Reverse: 5ʹ-TTCACATCCAGCACATCC-3ʹ | |
| β-catenin | Forward: 5ʹ-GAAGAGGCTGAAGGCAAAG-3ʹ |
| Reverse: 5ʹ-AGCAGAGGGAATCAAACA-3ʹ | |
| N-cadherin | Forward: 5ʹ-AGGATCAACCCCATACACCA-3ʹ |
| Reverse: 5ʹ-TGGTTTGACCACGGTGACTA-3ʹ | |
| β-actin | Forward: 5ʹ-GCGAGCATCCCCCAAAGTT-3ʹ |
| Reverse: 5ʹ-GGGCACGAAGGCTCATCATT-3ʹ |
| Gene . | Primer sequence . |
|---|---|
| Snai1 | Forward: 5ʹ-TTACCTTCCAGCAGCCCTAC-3ʹ |
| Reverse: 5ʹ-AGCCTTTCCCACTGTCCTC-3ʹ | |
| Twist | Forward: 5ʹ-CGACGACAGCCTGAGCAACA-3ʹ |
| Reverse: 5ʹ-CCACAGCCCGCAGACTTCTT-3ʹ | |
| Slug | Forward: 5ʹ-CCTCCATCTGACACCTCC-3ʹ |
| Reverse: 5ʹ-CCCAGGCTCACATATTCC-3ʹ | |
| Zeb1 | Forward: 5ʹ-AAGTGGCGGTAGATGGTA-3ʹ |
| Reverse: 5ʹ-TTGTAGCGACTGGATTTT-3ʹ | |
| Vimentin | Forward: 5ʹ-TTGAACGCAAAGTGGAATC-3ʹ |
| Reverse: 5ʹ-AGGTCAGGCTTGGAAACA-3ʹ | |
| E-cadherin | Forward: 5ʹ-CTGAGAACGAGGCTAACG-3ʹ |
| Reverse: 5ʹ-TTCACATCCAGCACATCC-3ʹ | |
| β-catenin | Forward: 5ʹ-GAAGAGGCTGAAGGCAAAG-3ʹ |
| Reverse: 5ʹ-AGCAGAGGGAATCAAACA-3ʹ | |
| N-cadherin | Forward: 5ʹ-AGGATCAACCCCATACACCA-3ʹ |
| Reverse: 5ʹ-TGGTTTGACCACGGTGACTA-3ʹ | |
| β-actin | Forward: 5ʹ-GCGAGCATCCCCCAAAGTT-3ʹ |
| Reverse: 5ʹ-GGGCACGAAGGCTCATCATT-3ʹ |
| Gene . | Primer sequence . |
|---|---|
| Snai1 | Forward: 5ʹ-TTACCTTCCAGCAGCCCTAC-3ʹ |
| Reverse: 5ʹ-AGCCTTTCCCACTGTCCTC-3ʹ | |
| Twist | Forward: 5ʹ-CGACGACAGCCTGAGCAACA-3ʹ |
| Reverse: 5ʹ-CCACAGCCCGCAGACTTCTT-3ʹ | |
| Slug | Forward: 5ʹ-CCTCCATCTGACACCTCC-3ʹ |
| Reverse: 5ʹ-CCCAGGCTCACATATTCC-3ʹ | |
| Zeb1 | Forward: 5ʹ-AAGTGGCGGTAGATGGTA-3ʹ |
| Reverse: 5ʹ-TTGTAGCGACTGGATTTT-3ʹ | |
| Vimentin | Forward: 5ʹ-TTGAACGCAAAGTGGAATC-3ʹ |
| Reverse: 5ʹ-AGGTCAGGCTTGGAAACA-3ʹ | |
| E-cadherin | Forward: 5ʹ-CTGAGAACGAGGCTAACG-3ʹ |
| Reverse: 5ʹ-TTCACATCCAGCACATCC-3ʹ | |
| β-catenin | Forward: 5ʹ-GAAGAGGCTGAAGGCAAAG-3ʹ |
| Reverse: 5ʹ-AGCAGAGGGAATCAAACA-3ʹ | |
| N-cadherin | Forward: 5ʹ-AGGATCAACCCCATACACCA-3ʹ |
| Reverse: 5ʹ-TGGTTTGACCACGGTGACTA-3ʹ | |
| β-actin | Forward: 5ʹ-GCGAGCATCCCCCAAAGTT-3ʹ |
| Reverse: 5ʹ-GGGCACGAAGGCTCATCATT-3ʹ |
| Gene . | Primer sequence . |
|---|---|
| Snai1 | Forward: 5ʹ-TTACCTTCCAGCAGCCCTAC-3ʹ |
| Reverse: 5ʹ-AGCCTTTCCCACTGTCCTC-3ʹ | |
| Twist | Forward: 5ʹ-CGACGACAGCCTGAGCAACA-3ʹ |
| Reverse: 5ʹ-CCACAGCCCGCAGACTTCTT-3ʹ | |
| Slug | Forward: 5ʹ-CCTCCATCTGACACCTCC-3ʹ |
| Reverse: 5ʹ-CCCAGGCTCACATATTCC-3ʹ | |
| Zeb1 | Forward: 5ʹ-AAGTGGCGGTAGATGGTA-3ʹ |
| Reverse: 5ʹ-TTGTAGCGACTGGATTTT-3ʹ | |
| Vimentin | Forward: 5ʹ-TTGAACGCAAAGTGGAATC-3ʹ |
| Reverse: 5ʹ-AGGTCAGGCTTGGAAACA-3ʹ | |
| E-cadherin | Forward: 5ʹ-CTGAGAACGAGGCTAACG-3ʹ |
| Reverse: 5ʹ-TTCACATCCAGCACATCC-3ʹ | |
| β-catenin | Forward: 5ʹ-GAAGAGGCTGAAGGCAAAG-3ʹ |
| Reverse: 5ʹ-AGCAGAGGGAATCAAACA-3ʹ | |
| N-cadherin | Forward: 5ʹ-AGGATCAACCCCATACACCA-3ʹ |
| Reverse: 5ʹ-TGGTTTGACCACGGTGACTA-3ʹ | |
| β-actin | Forward: 5ʹ-GCGAGCATCCCCCAAAGTT-3ʹ |
| Reverse: 5ʹ-GGGCACGAAGGCTCATCATT-3ʹ |
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