Table 2.
Open in new tab

Genotypes and MICCip of evolved isolates

StrainParental genotypeaAcquired mutationsbMICCip (mg/L)
CH1464WTWT0.015
CH6295marR I18fsparental strain0.06
CH6881marR I18fs

marA T125<>IS1

citC ntA-171T

0.023
CH6882marR I18fs

gltP ntA-115T

yqeG I42L

rpoC H419P

dosC ntT-259<>IS5

nanM I10<>IS2

0.045
CH6883marR I18fs

lon E4E (GAG>GAA)

rhaD ntA-143C

rhaD ntC-217T

0.03
CH6884marR I18fs

marA M63K

arcA P58R

sapD A195P

gcvP V885M

phnN L20L (CTG>CTA)

yjdA A726P

0.015
CH6885marR I18fs

lon ntC-26T

ydeN D508A

ygiF A294A (GCG>GCT)

0.03
CH6886marR I18fslon E4E (GAG>GAA)0.03
CH6969marR I18fs

ΔmarA (1.9 kb)

rbsR G165fs

0.023
CH6970marR I18fsarcA P58R0.06
CH6971marR I18fsΔmarA (4.8 kb)0.015
CH6972marR I18fs

marA Y37H

gltP ntA-115T

0.023
CH6300marR C51fsparental strain0.045
CH6921marR C51fsΔmarA (1.1 kb)0.015
CH6922marR C51fs

arcA R16H

gltP ntA-115T

malE W184*

0.03
CH6923marR C51fsarcA L65F0.045
CH6924marR C51fs

lon E4E (GAG>GAA)

gltP ntA-115T

0.023
CH6925marR C51fs

marA S32*

gltP ntA-115T

0.015
CH6926marR C51fs

marA I18F

gltP ntA-115T

ΔppsA (3 kb)

0.015
CH6973marR C51fs

gltP ntA-115T

gltB R117H

arcB S83fs

0.045
CH6974marR C51fs

arcA G59A

gltP ntA-115T

0.045
CH6975marR C51fsΔmarA (5.0 kb)0.015
CH6976marR C51fs

arcA I205L

gltP ntA-115T

leuX ntG-22T

0.045
StrainParental genotypeaAcquired mutationsbMICCip (mg/L)
CH1464WTWT0.015
CH6295marR I18fsparental strain0.06
CH6881marR I18fs

marA T125<>IS1

citC ntA-171T

0.023
CH6882marR I18fs

gltP ntA-115T

yqeG I42L

rpoC H419P

dosC ntT-259<>IS5

nanM I10<>IS2

0.045
CH6883marR I18fs

lon E4E (GAG>GAA)

rhaD ntA-143C

rhaD ntC-217T

0.03
CH6884marR I18fs

marA M63K

arcA P58R

sapD A195P

gcvP V885M

phnN L20L (CTG>CTA)

yjdA A726P

0.015
CH6885marR I18fs

lon ntC-26T

ydeN D508A

ygiF A294A (GCG>GCT)

0.03
CH6886marR I18fslon E4E (GAG>GAA)0.03
CH6969marR I18fs

ΔmarA (1.9 kb)

rbsR G165fs

0.023
CH6970marR I18fsarcA P58R0.06
CH6971marR I18fsΔmarA (4.8 kb)0.015
CH6972marR I18fs

marA Y37H

gltP ntA-115T

0.023
CH6300marR C51fsparental strain0.045
CH6921marR C51fsΔmarA (1.1 kb)0.015
CH6922marR C51fs

arcA R16H

gltP ntA-115T

malE W184*

0.03
CH6923marR C51fsarcA L65F0.045
CH6924marR C51fs

lon E4E (GAG>GAA)

gltP ntA-115T

0.023
CH6925marR C51fs

marA S32*

gltP ntA-115T

0.015
CH6926marR C51fs

marA I18F

gltP ntA-115T

ΔppsA (3 kb)

0.015
CH6973marR C51fs

gltP ntA-115T

gltB R117H

arcB S83fs

0.045
CH6974marR C51fs

arcA G59A

gltP ntA-115T

0.045
CH6975marR C51fsΔmarA (5.0 kb)0.015
CH6976marR C51fs

arcA I205L

gltP ntA-115T

leuX ntG-22T

0.045

An asterisk denotes a stop codon.

a

All isolates with the marR I18fs mutation also carry an mngA duplication (nt −49 to −44) mutation in the chromosome.

b

See Table S1 for details on ΔmarA deletions. Numbers in parentheses show total size of deletions.

Table 2.
Open in new tab

Genotypes and MICCip of evolved isolates

StrainParental genotypeaAcquired mutationsbMICCip (mg/L)
CH1464WTWT0.015
CH6295marR I18fsparental strain0.06
CH6881marR I18fs

marA T125<>IS1

citC ntA-171T

0.023
CH6882marR I18fs

gltP ntA-115T

yqeG I42L

rpoC H419P

dosC ntT-259<>IS5

nanM I10<>IS2

0.045
CH6883marR I18fs

lon E4E (GAG>GAA)

rhaD ntA-143C

rhaD ntC-217T

0.03
CH6884marR I18fs

marA M63K

arcA P58R

sapD A195P

gcvP V885M

phnN L20L (CTG>CTA)

yjdA A726P

0.015
CH6885marR I18fs

lon ntC-26T

ydeN D508A

ygiF A294A (GCG>GCT)

0.03
CH6886marR I18fslon E4E (GAG>GAA)0.03
CH6969marR I18fs

ΔmarA (1.9 kb)

rbsR G165fs

0.023
CH6970marR I18fsarcA P58R0.06
CH6971marR I18fsΔmarA (4.8 kb)0.015
CH6972marR I18fs

marA Y37H

gltP ntA-115T

0.023
CH6300marR C51fsparental strain0.045
CH6921marR C51fsΔmarA (1.1 kb)0.015
CH6922marR C51fs

arcA R16H

gltP ntA-115T

malE W184*

0.03
CH6923marR C51fsarcA L65F0.045
CH6924marR C51fs

lon E4E (GAG>GAA)

gltP ntA-115T

0.023
CH6925marR C51fs

marA S32*

gltP ntA-115T

0.015
CH6926marR C51fs

marA I18F

gltP ntA-115T

ΔppsA (3 kb)

0.015
CH6973marR C51fs

gltP ntA-115T

gltB R117H

arcB S83fs

0.045
CH6974marR C51fs

arcA G59A

gltP ntA-115T

0.045
CH6975marR C51fsΔmarA (5.0 kb)0.015
CH6976marR C51fs

arcA I205L

gltP ntA-115T

leuX ntG-22T

0.045
StrainParental genotypeaAcquired mutationsbMICCip (mg/L)
CH1464WTWT0.015
CH6295marR I18fsparental strain0.06
CH6881marR I18fs

marA T125<>IS1

citC ntA-171T

0.023
CH6882marR I18fs

gltP ntA-115T

yqeG I42L

rpoC H419P

dosC ntT-259<>IS5

nanM I10<>IS2

0.045
CH6883marR I18fs

lon E4E (GAG>GAA)

rhaD ntA-143C

rhaD ntC-217T

0.03
CH6884marR I18fs

marA M63K

arcA P58R

sapD A195P

gcvP V885M

phnN L20L (CTG>CTA)

yjdA A726P

0.015
CH6885marR I18fs

lon ntC-26T

ydeN D508A

ygiF A294A (GCG>GCT)

0.03
CH6886marR I18fslon E4E (GAG>GAA)0.03
CH6969marR I18fs

ΔmarA (1.9 kb)

rbsR G165fs

0.023
CH6970marR I18fsarcA P58R0.06
CH6971marR I18fsΔmarA (4.8 kb)0.015
CH6972marR I18fs

marA Y37H

gltP ntA-115T

0.023
CH6300marR C51fsparental strain0.045
CH6921marR C51fsΔmarA (1.1 kb)0.015
CH6922marR C51fs

arcA R16H

gltP ntA-115T

malE W184*

0.03
CH6923marR C51fsarcA L65F0.045
CH6924marR C51fs

lon E4E (GAG>GAA)

gltP ntA-115T

0.023
CH6925marR C51fs

marA S32*

gltP ntA-115T

0.015
CH6926marR C51fs

marA I18F

gltP ntA-115T

ΔppsA (3 kb)

0.015
CH6973marR C51fs

gltP ntA-115T

gltB R117H

arcB S83fs

0.045
CH6974marR C51fs

arcA G59A

gltP ntA-115T

0.045
CH6975marR C51fsΔmarA (5.0 kb)0.015
CH6976marR C51fs

arcA I205L

gltP ntA-115T

leuX ntG-22T

0.045

An asterisk denotes a stop codon.

a

All isolates with the marR I18fs mutation also carry an mngA duplication (nt −49 to −44) mutation in the chromosome.

b

See Table S1 for details on ΔmarA deletions. Numbers in parentheses show total size of deletions.

Close
This Feature Is Available To Subscribers Only

Sign In or Create an Account

Close

This PDF is available to Subscribers Only

View Article Abstract & Purchase Options

For full access to this pdf, sign in to an existing account, or purchase an annual subscription.

Close