Production of IL8, IL10, and TNF-α by PBMC in response to ConA, PMA, or LPS stimulation in 42-d-old piglets fed different experimental diets1
Variable . | Stimulation . | DT . | . | . | . | SEM . | P-value . |
---|---|---|---|---|---|---|---|
. | . | CTRL . | ATB . | CKTL . | CKTL + COL . | . | . |
IL8 | ConA | 0.89 | 0.75 | 0.60 | 0.57 | 0.28 | 0.71 |
LPS | 2.57 | 3.21 | 2.56 | 2.30 | 0.65 | 0.54 | |
PMA | 2.75 | 2.92 | 2.80 | 2.08 | 0.54 | 0.47 | |
IL10 | ConA | 3.52 | 4.17 | 3.18 | 2.43 | 0.77 | 0.12 |
LPS | 4.17 | 4.93 | 4.09 | 3.38 | 0.85 | 0.25 | |
PMA | 3.58 | 4.36 | 3.65 | 2.76 | 0.78 | 0.25 | |
TNF-α | ConA | 2.39ab | 2.85a | 2.08ab | 1.59b | 0.43 | 0.06 |
LPS | 2.10 | 2.87 | 2.35 | 1.96 | 0.41 | 0.19 | |
PMA | 3.36 | 3.97 | 3.71 | 2.60 | 0.56 | 0.06 |
Variable . | Stimulation . | DT . | . | . | . | SEM . | P-value . |
---|---|---|---|---|---|---|---|
. | . | CTRL . | ATB . | CKTL . | CKTL + COL . | . | . |
IL8 | ConA | 0.89 | 0.75 | 0.60 | 0.57 | 0.28 | 0.71 |
LPS | 2.57 | 3.21 | 2.56 | 2.30 | 0.65 | 0.54 | |
PMA | 2.75 | 2.92 | 2.80 | 2.08 | 0.54 | 0.47 | |
IL10 | ConA | 3.52 | 4.17 | 3.18 | 2.43 | 0.77 | 0.12 |
LPS | 4.17 | 4.93 | 4.09 | 3.38 | 0.85 | 0.25 | |
PMA | 3.58 | 4.36 | 3.65 | 2.76 | 0.78 | 0.25 | |
TNF-α | ConA | 2.39ab | 2.85a | 2.08ab | 1.59b | 0.43 | 0.06 |
LPS | 2.10 | 2.87 | 2.35 | 1.96 | 0.41 | 0.19 | |
PMA | 3.36 | 3.97 | 3.71 | 2.60 | 0.56 | 0.06 |
1Data are reported as delta values relative to the basal condition and expressed as log2 pg/mL.
a,bResults of the Tukey’s test: within a row, means without a common superscript differ (P < 0.05).
Production of IL8, IL10, and TNF-α by PBMC in response to ConA, PMA, or LPS stimulation in 42-d-old piglets fed different experimental diets1
Variable . | Stimulation . | DT . | . | . | . | SEM . | P-value . |
---|---|---|---|---|---|---|---|
. | . | CTRL . | ATB . | CKTL . | CKTL + COL . | . | . |
IL8 | ConA | 0.89 | 0.75 | 0.60 | 0.57 | 0.28 | 0.71 |
LPS | 2.57 | 3.21 | 2.56 | 2.30 | 0.65 | 0.54 | |
PMA | 2.75 | 2.92 | 2.80 | 2.08 | 0.54 | 0.47 | |
IL10 | ConA | 3.52 | 4.17 | 3.18 | 2.43 | 0.77 | 0.12 |
LPS | 4.17 | 4.93 | 4.09 | 3.38 | 0.85 | 0.25 | |
PMA | 3.58 | 4.36 | 3.65 | 2.76 | 0.78 | 0.25 | |
TNF-α | ConA | 2.39ab | 2.85a | 2.08ab | 1.59b | 0.43 | 0.06 |
LPS | 2.10 | 2.87 | 2.35 | 1.96 | 0.41 | 0.19 | |
PMA | 3.36 | 3.97 | 3.71 | 2.60 | 0.56 | 0.06 |
Variable . | Stimulation . | DT . | . | . | . | SEM . | P-value . |
---|---|---|---|---|---|---|---|
. | . | CTRL . | ATB . | CKTL . | CKTL + COL . | . | . |
IL8 | ConA | 0.89 | 0.75 | 0.60 | 0.57 | 0.28 | 0.71 |
LPS | 2.57 | 3.21 | 2.56 | 2.30 | 0.65 | 0.54 | |
PMA | 2.75 | 2.92 | 2.80 | 2.08 | 0.54 | 0.47 | |
IL10 | ConA | 3.52 | 4.17 | 3.18 | 2.43 | 0.77 | 0.12 |
LPS | 4.17 | 4.93 | 4.09 | 3.38 | 0.85 | 0.25 | |
PMA | 3.58 | 4.36 | 3.65 | 2.76 | 0.78 | 0.25 | |
TNF-α | ConA | 2.39ab | 2.85a | 2.08ab | 1.59b | 0.43 | 0.06 |
LPS | 2.10 | 2.87 | 2.35 | 1.96 | 0.41 | 0.19 | |
PMA | 3.36 | 3.97 | 3.71 | 2.60 | 0.56 | 0.06 |
1Data are reported as delta values relative to the basal condition and expressed as log2 pg/mL.
a,bResults of the Tukey’s test: within a row, means without a common superscript differ (P < 0.05).
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