Reactivity of the VHHs Against VLPs of Representative Strains from Different Genotypes
| VLP Genotype . | Strain . | VHHs . | ||||
|---|---|---|---|---|---|---|
| . | . | 6A2 . | 7C6 . | 1A6 . | 1E4 . | Heterodimer 7C6-1E4 . |
| GI.4 | Chiba407_1987 | − | − | − | − | − |
| GI.6 | WUG1_2000 | − | − | − | − | − |
| GII.2 | TokyoMK04_2004 | − | +++ | +++ | − | +++ |
| GII.3 | SaitamaU201_1998 | − | +++ | +++ | − | +++ |
| Osaka (FB1650) | − | ++ | ++ | − | +++ | |
| GII.4 | Saga1_2006 | +++ | ++ | ± | − | +++ |
| Sakai08-403_2006b | +++ | +++ | ± | − | +++ | |
| Sydney_2012 | +++ | ++ | ± | − | +++ | |
| GII.6 | Ueno7K_1994 | − | ++ | − | − | ++ |
| GII.17 | Kawasaki308 | − | ++ | +++ | +++ | +++ |
| VLP Genotype . | Strain . | VHHs . | ||||
|---|---|---|---|---|---|---|
| . | . | 6A2 . | 7C6 . | 1A6 . | 1E4 . | Heterodimer 7C6-1E4 . |
| GI.4 | Chiba407_1987 | − | − | − | − | − |
| GI.6 | WUG1_2000 | − | − | − | − | − |
| GII.2 | TokyoMK04_2004 | − | +++ | +++ | − | +++ |
| GII.3 | SaitamaU201_1998 | − | +++ | +++ | − | +++ |
| Osaka (FB1650) | − | ++ | ++ | − | +++ | |
| GII.4 | Saga1_2006 | +++ | ++ | ± | − | +++ |
| Sakai08-403_2006b | +++ | +++ | ± | − | +++ | |
| Sydney_2012 | +++ | ++ | ± | − | +++ | |
| GII.6 | Ueno7K_1994 | − | ++ | − | − | ++ |
| GII.17 | Kawasaki308 | − | ++ | +++ | +++ | +++ |
Abbreviations: VHH, variable-domain heavy-chain antibody fragment; VLP, virus-like particle.
−, absence of reaction (100 nM VHH, absorbance at 450 nm < 0.2); ±, detectable reaction (100 nM VHH, absorbance at 450 nm > 0.2); +, low reaction (100 nM VHH, absorbance at 450nm > 1.0); ++, moderate reaction (10 nM VHH, absorbance at 450 nm > 1.0); +++, high reaction (1 nM VHH, absorbance at 450 nm > 1.0).
Reactivity of the VHHs Against VLPs of Representative Strains from Different Genotypes
| VLP Genotype . | Strain . | VHHs . | ||||
|---|---|---|---|---|---|---|
| . | . | 6A2 . | 7C6 . | 1A6 . | 1E4 . | Heterodimer 7C6-1E4 . |
| GI.4 | Chiba407_1987 | − | − | − | − | − |
| GI.6 | WUG1_2000 | − | − | − | − | − |
| GII.2 | TokyoMK04_2004 | − | +++ | +++ | − | +++ |
| GII.3 | SaitamaU201_1998 | − | +++ | +++ | − | +++ |
| Osaka (FB1650) | − | ++ | ++ | − | +++ | |
| GII.4 | Saga1_2006 | +++ | ++ | ± | − | +++ |
| Sakai08-403_2006b | +++ | +++ | ± | − | +++ | |
| Sydney_2012 | +++ | ++ | ± | − | +++ | |
| GII.6 | Ueno7K_1994 | − | ++ | − | − | ++ |
| GII.17 | Kawasaki308 | − | ++ | +++ | +++ | +++ |
| VLP Genotype . | Strain . | VHHs . | ||||
|---|---|---|---|---|---|---|
| . | . | 6A2 . | 7C6 . | 1A6 . | 1E4 . | Heterodimer 7C6-1E4 . |
| GI.4 | Chiba407_1987 | − | − | − | − | − |
| GI.6 | WUG1_2000 | − | − | − | − | − |
| GII.2 | TokyoMK04_2004 | − | +++ | +++ | − | +++ |
| GII.3 | SaitamaU201_1998 | − | +++ | +++ | − | +++ |
| Osaka (FB1650) | − | ++ | ++ | − | +++ | |
| GII.4 | Saga1_2006 | +++ | ++ | ± | − | +++ |
| Sakai08-403_2006b | +++ | +++ | ± | − | +++ | |
| Sydney_2012 | +++ | ++ | ± | − | +++ | |
| GII.6 | Ueno7K_1994 | − | ++ | − | − | ++ |
| GII.17 | Kawasaki308 | − | ++ | +++ | +++ | +++ |
Abbreviations: VHH, variable-domain heavy-chain antibody fragment; VLP, virus-like particle.
−, absence of reaction (100 nM VHH, absorbance at 450 nm < 0.2); ±, detectable reaction (100 nM VHH, absorbance at 450 nm > 0.2); +, low reaction (100 nM VHH, absorbance at 450nm > 1.0); ++, moderate reaction (10 nM VHH, absorbance at 450 nm > 1.0); +++, high reaction (1 nM VHH, absorbance at 450 nm > 1.0).
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