| Strains . | Description . | MIC (mg/L) of tetracycline antimicrobials . | |||||
|---|---|---|---|---|---|---|---|
| TET . | DOX . | MIN . | TGC . | ERA . | OMA . | ||
| M. phaeus 18QD1AZ29W | the original tet(X6) | 64 | 32 | 32 | 32 | 32 | 128 |
| P. mirabilis 18QD2AZ3W | 4 amino acid substitutions compared with the original tet(X6) | 128 | 128 | 128 | 16 | 16 | 64 |
| A. johnsonii 18QD2AZ57W | 11 amino acid substitutions compared with the original tet(X6) | 128 | 64 | 16 | 32 | 32 | 128 |
| A. lwoffii 18QD2AZ41W | 11 amino acid substitutions compared with the original tet(X6) | 64 | 32 | 16 | 16 | 16 | 64 |
| A. lwoffii 18QD2AZ28W | 11 amino acid substitutions compared with the original tet(X6) | 64 | 32 | 16 | 16 | 16 | 64 |
| P. cibarius 17SZRF8EW | 16 amino acid substitutions compared with the original tet(X6) | 128 | 128 | 128 | 16 | 16 | 64 |
| P. cibarius 17SZRF19EW | 16 amino acid substitutions compared with the original tet(X6) | 128 | 128 | 64 | 16 | 16 | 64 |
| P. cibarius 17SZRZ8EW | 16 amino acid substitutions compared with the original tet(X6) | 128 | 128 | 64 | 16 | 16 | 64 |
| E. coli DH5α+pAM401 | recipient for the tet(X6) gene | 1 | 2 | 2 | 0.125 | 0.25 | 1 |
| E. coli DH5α- pAM401-tet(X6) | transformants of the original tet(X6) | 4 | 4 | 2 | 0.5 | 1 | 2 |
| E. coli DH5α- pAM401-tet(X6)-4 | transformants of the tet(X6) variant with 4 amino acid substitutions | 4 | 4 | 8 | 0.5 | 1 | 4 |
| E. coli DH5α- pAM401-tet(X6)-11 | transformants of the tet(X6) variant with 11 amino acid substitutions | 4 | 8 | 8 | 0.5 | 1 | 4 |
| E. coli DH5α- pAM401-tet(X6)-16 | transformants of the tet(X6) variant with 16 amino acid substitutions | 4 | 8 | 8 | 0.5 | 1 | 4 |
| Strains . | Description . | MIC (mg/L) of tetracycline antimicrobials . | |||||
|---|---|---|---|---|---|---|---|
| TET . | DOX . | MIN . | TGC . | ERA . | OMA . | ||
| M. phaeus 18QD1AZ29W | the original tet(X6) | 64 | 32 | 32 | 32 | 32 | 128 |
| P. mirabilis 18QD2AZ3W | 4 amino acid substitutions compared with the original tet(X6) | 128 | 128 | 128 | 16 | 16 | 64 |
| A. johnsonii 18QD2AZ57W | 11 amino acid substitutions compared with the original tet(X6) | 128 | 64 | 16 | 32 | 32 | 128 |
| A. lwoffii 18QD2AZ41W | 11 amino acid substitutions compared with the original tet(X6) | 64 | 32 | 16 | 16 | 16 | 64 |
| A. lwoffii 18QD2AZ28W | 11 amino acid substitutions compared with the original tet(X6) | 64 | 32 | 16 | 16 | 16 | 64 |
| P. cibarius 17SZRF8EW | 16 amino acid substitutions compared with the original tet(X6) | 128 | 128 | 128 | 16 | 16 | 64 |
| P. cibarius 17SZRF19EW | 16 amino acid substitutions compared with the original tet(X6) | 128 | 128 | 64 | 16 | 16 | 64 |
| P. cibarius 17SZRZ8EW | 16 amino acid substitutions compared with the original tet(X6) | 128 | 128 | 64 | 16 | 16 | 64 |
| E. coli DH5α+pAM401 | recipient for the tet(X6) gene | 1 | 2 | 2 | 0.125 | 0.25 | 1 |
| E. coli DH5α- pAM401-tet(X6) | transformants of the original tet(X6) | 4 | 4 | 2 | 0.5 | 1 | 2 |
| E. coli DH5α- pAM401-tet(X6)-4 | transformants of the tet(X6) variant with 4 amino acid substitutions | 4 | 4 | 8 | 0.5 | 1 | 4 |
| E. coli DH5α- pAM401-tet(X6)-11 | transformants of the tet(X6) variant with 11 amino acid substitutions | 4 | 8 | 8 | 0.5 | 1 | 4 |
| E. coli DH5α- pAM401-tet(X6)-16 | transformants of the tet(X6) variant with 16 amino acid substitutions | 4 | 8 | 8 | 0.5 | 1 | 4 |
TET, tetracycline; DOX, doxycycline; MIN, minocycline; TGC, tigecycline; ERA, eravacycline; OMA, omadacycline.
| Strains . | Description . | MIC (mg/L) of tetracycline antimicrobials . | |||||
|---|---|---|---|---|---|---|---|
| TET . | DOX . | MIN . | TGC . | ERA . | OMA . | ||
| M. phaeus 18QD1AZ29W | the original tet(X6) | 64 | 32 | 32 | 32 | 32 | 128 |
| P. mirabilis 18QD2AZ3W | 4 amino acid substitutions compared with the original tet(X6) | 128 | 128 | 128 | 16 | 16 | 64 |
| A. johnsonii 18QD2AZ57W | 11 amino acid substitutions compared with the original tet(X6) | 128 | 64 | 16 | 32 | 32 | 128 |
| A. lwoffii 18QD2AZ41W | 11 amino acid substitutions compared with the original tet(X6) | 64 | 32 | 16 | 16 | 16 | 64 |
| A. lwoffii 18QD2AZ28W | 11 amino acid substitutions compared with the original tet(X6) | 64 | 32 | 16 | 16 | 16 | 64 |
| P. cibarius 17SZRF8EW | 16 amino acid substitutions compared with the original tet(X6) | 128 | 128 | 128 | 16 | 16 | 64 |
| P. cibarius 17SZRF19EW | 16 amino acid substitutions compared with the original tet(X6) | 128 | 128 | 64 | 16 | 16 | 64 |
| P. cibarius 17SZRZ8EW | 16 amino acid substitutions compared with the original tet(X6) | 128 | 128 | 64 | 16 | 16 | 64 |
| E. coli DH5α+pAM401 | recipient for the tet(X6) gene | 1 | 2 | 2 | 0.125 | 0.25 | 1 |
| E. coli DH5α- pAM401-tet(X6) | transformants of the original tet(X6) | 4 | 4 | 2 | 0.5 | 1 | 2 |
| E. coli DH5α- pAM401-tet(X6)-4 | transformants of the tet(X6) variant with 4 amino acid substitutions | 4 | 4 | 8 | 0.5 | 1 | 4 |
| E. coli DH5α- pAM401-tet(X6)-11 | transformants of the tet(X6) variant with 11 amino acid substitutions | 4 | 8 | 8 | 0.5 | 1 | 4 |
| E. coli DH5α- pAM401-tet(X6)-16 | transformants of the tet(X6) variant with 16 amino acid substitutions | 4 | 8 | 8 | 0.5 | 1 | 4 |
| Strains . | Description . | MIC (mg/L) of tetracycline antimicrobials . | |||||
|---|---|---|---|---|---|---|---|
| TET . | DOX . | MIN . | TGC . | ERA . | OMA . | ||
| M. phaeus 18QD1AZ29W | the original tet(X6) | 64 | 32 | 32 | 32 | 32 | 128 |
| P. mirabilis 18QD2AZ3W | 4 amino acid substitutions compared with the original tet(X6) | 128 | 128 | 128 | 16 | 16 | 64 |
| A. johnsonii 18QD2AZ57W | 11 amino acid substitutions compared with the original tet(X6) | 128 | 64 | 16 | 32 | 32 | 128 |
| A. lwoffii 18QD2AZ41W | 11 amino acid substitutions compared with the original tet(X6) | 64 | 32 | 16 | 16 | 16 | 64 |
| A. lwoffii 18QD2AZ28W | 11 amino acid substitutions compared with the original tet(X6) | 64 | 32 | 16 | 16 | 16 | 64 |
| P. cibarius 17SZRF8EW | 16 amino acid substitutions compared with the original tet(X6) | 128 | 128 | 128 | 16 | 16 | 64 |
| P. cibarius 17SZRF19EW | 16 amino acid substitutions compared with the original tet(X6) | 128 | 128 | 64 | 16 | 16 | 64 |
| P. cibarius 17SZRZ8EW | 16 amino acid substitutions compared with the original tet(X6) | 128 | 128 | 64 | 16 | 16 | 64 |
| E. coli DH5α+pAM401 | recipient for the tet(X6) gene | 1 | 2 | 2 | 0.125 | 0.25 | 1 |
| E. coli DH5α- pAM401-tet(X6) | transformants of the original tet(X6) | 4 | 4 | 2 | 0.5 | 1 | 2 |
| E. coli DH5α- pAM401-tet(X6)-4 | transformants of the tet(X6) variant with 4 amino acid substitutions | 4 | 4 | 8 | 0.5 | 1 | 4 |
| E. coli DH5α- pAM401-tet(X6)-11 | transformants of the tet(X6) variant with 11 amino acid substitutions | 4 | 8 | 8 | 0.5 | 1 | 4 |
| E. coli DH5α- pAM401-tet(X6)-16 | transformants of the tet(X6) variant with 16 amino acid substitutions | 4 | 8 | 8 | 0.5 | 1 | 4 |
TET, tetracycline; DOX, doxycycline; MIN, minocycline; TGC, tigecycline; ERA, eravacycline; OMA, omadacycline.
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