Table 1.
Open in new tab

Strains used in this study

StrainGenotypeParent strainSource/technique
MG1655uup+ radD+ xerD+–(125,126)
STL7180recA::catMG1655(92)
STL7212Δuup::FRT kanMG1655Lambda Red recombination
STL8027ΔradD::FRT catMG1655Lambda Red recombination
STL21852ΔradD::FRT catMG1655Transduction of MG1655 with P1 grown on STL8027
STL21884uup::FRT kanMG1655Transduction of MG1655 with P1 grown on STL7212
STL21912ΔradD::FRTMG1655CatS derivative of STL21852 after pCP20 transformation and curing
STL21712recA::catMG1655Transduction of MG1655 with P1 grown on STL7180
STL21936Δuup::FRT kan ΔradD::FRTMG1655Transduction of STL21912 with P1 grown on STL7212
STL21954recA::cat ΔradD::FRT catMG1655Transduction of STL21912 with P1 grown on STL7180
STL21932recA::cat Δuup::FRT kanMG1655Transduction of STL21884 with P1 grown on STL7180
STL21969recA::cat Δuup::FRT kan ΔradD::FRTMG1655Transduction of STL21936 with P1 grown on STL7180
ZJR01△uup°MG1655Transduction of MG1655 with P1 grown on EAW242
ZJR04△uup△radDMG1655Transduction of ZJR01 with P1 grown on EAW232
EAW526ΔradDMG1655Transduction of MG1655 with P1 grown on EAW232
EAW1133ΔxerDMG1655Lambda RED recombination
EAW1136ΔradD +Δlac IZYA +Δuup + ΔxerDEAW1132Transduction of EAW1132 with P1 grown on EAW1133
EAW1140AmpR &Tet repeat +ΔxerDSTL695Transduction of STL695 with P1 grown on EAW1133
EAW1145AmpR &Tet repeat +Δuup +ΔradD +ΔxerDEAW1139Transduction of EAW1139 with P1 grown on EAW1133
EAW242founder+Δe14+ΔuupMG1655Lambda RED recombination
EAW232founder+Δe14+ΔradDMG1655Lambda RED recombination
EAW1132ΔradD +Δlac IZYA +ΔuupEAW1100Transduction of EAW1100 with P1 grown on EAW242
EAW1100ΔradD +Δlac IZYAEAW526Transduction of EAW526 with P1 grown on EAW408
EAW408Δlac IZYAMG1655Lambda RED recombination
EAW1139AmpR &Tet repeat +Δuup +ΔradDZJR05Transduction of ZJR05 with P1 grown on EAW232
STL695AmpR &Tet repeatAB1157(89)
ZJR05AmpR &Tet repeat +ΔuupSTL695Transduction of STL695 with P1 grown on EAW242
StrainGenotypeParent strainSource/technique
MG1655uup+ radD+ xerD+–(125,126)
STL7180recA::catMG1655(92)
STL7212Δuup::FRT kanMG1655Lambda Red recombination
STL8027ΔradD::FRT catMG1655Lambda Red recombination
STL21852ΔradD::FRT catMG1655Transduction of MG1655 with P1 grown on STL8027
STL21884uup::FRT kanMG1655Transduction of MG1655 with P1 grown on STL7212
STL21912ΔradD::FRTMG1655CatS derivative of STL21852 after pCP20 transformation and curing
STL21712recA::catMG1655Transduction of MG1655 with P1 grown on STL7180
STL21936Δuup::FRT kan ΔradD::FRTMG1655Transduction of STL21912 with P1 grown on STL7212
STL21954recA::cat ΔradD::FRT catMG1655Transduction of STL21912 with P1 grown on STL7180
STL21932recA::cat Δuup::FRT kanMG1655Transduction of STL21884 with P1 grown on STL7180
STL21969recA::cat Δuup::FRT kan ΔradD::FRTMG1655Transduction of STL21936 with P1 grown on STL7180
ZJR01△uup°MG1655Transduction of MG1655 with P1 grown on EAW242
ZJR04△uup△radDMG1655Transduction of ZJR01 with P1 grown on EAW232
EAW526ΔradDMG1655Transduction of MG1655 with P1 grown on EAW232
EAW1133ΔxerDMG1655Lambda RED recombination
EAW1136ΔradD +Δlac IZYA +Δuup + ΔxerDEAW1132Transduction of EAW1132 with P1 grown on EAW1133
EAW1140AmpR &Tet repeat +ΔxerDSTL695Transduction of STL695 with P1 grown on EAW1133
EAW1145AmpR &Tet repeat +Δuup +ΔradD +ΔxerDEAW1139Transduction of EAW1139 with P1 grown on EAW1133
EAW242founder+Δe14+ΔuupMG1655Lambda RED recombination
EAW232founder+Δe14+ΔradDMG1655Lambda RED recombination
EAW1132ΔradD +Δlac IZYA +ΔuupEAW1100Transduction of EAW1100 with P1 grown on EAW242
EAW1100ΔradD +Δlac IZYAEAW526Transduction of EAW526 with P1 grown on EAW408
EAW408Δlac IZYAMG1655Lambda RED recombination
EAW1139AmpR &Tet repeat +Δuup +ΔradDZJR05Transduction of ZJR05 with P1 grown on EAW232
STL695AmpR &Tet repeatAB1157(89)
ZJR05AmpR &Tet repeat +ΔuupSTL695Transduction of STL695 with P1 grown on EAW242
Table 1.
Open in new tab

Strains used in this study

StrainGenotypeParent strainSource/technique
MG1655uup+ radD+ xerD+–(125,126)
STL7180recA::catMG1655(92)
STL7212Δuup::FRT kanMG1655Lambda Red recombination
STL8027ΔradD::FRT catMG1655Lambda Red recombination
STL21852ΔradD::FRT catMG1655Transduction of MG1655 with P1 grown on STL8027
STL21884uup::FRT kanMG1655Transduction of MG1655 with P1 grown on STL7212
STL21912ΔradD::FRTMG1655CatS derivative of STL21852 after pCP20 transformation and curing
STL21712recA::catMG1655Transduction of MG1655 with P1 grown on STL7180
STL21936Δuup::FRT kan ΔradD::FRTMG1655Transduction of STL21912 with P1 grown on STL7212
STL21954recA::cat ΔradD::FRT catMG1655Transduction of STL21912 with P1 grown on STL7180
STL21932recA::cat Δuup::FRT kanMG1655Transduction of STL21884 with P1 grown on STL7180
STL21969recA::cat Δuup::FRT kan ΔradD::FRTMG1655Transduction of STL21936 with P1 grown on STL7180
ZJR01△uup°MG1655Transduction of MG1655 with P1 grown on EAW242
ZJR04△uup△radDMG1655Transduction of ZJR01 with P1 grown on EAW232
EAW526ΔradDMG1655Transduction of MG1655 with P1 grown on EAW232
EAW1133ΔxerDMG1655Lambda RED recombination
EAW1136ΔradD +Δlac IZYA +Δuup + ΔxerDEAW1132Transduction of EAW1132 with P1 grown on EAW1133
EAW1140AmpR &Tet repeat +ΔxerDSTL695Transduction of STL695 with P1 grown on EAW1133
EAW1145AmpR &Tet repeat +Δuup +ΔradD +ΔxerDEAW1139Transduction of EAW1139 with P1 grown on EAW1133
EAW242founder+Δe14+ΔuupMG1655Lambda RED recombination
EAW232founder+Δe14+ΔradDMG1655Lambda RED recombination
EAW1132ΔradD +Δlac IZYA +ΔuupEAW1100Transduction of EAW1100 with P1 grown on EAW242
EAW1100ΔradD +Δlac IZYAEAW526Transduction of EAW526 with P1 grown on EAW408
EAW408Δlac IZYAMG1655Lambda RED recombination
EAW1139AmpR &Tet repeat +Δuup +ΔradDZJR05Transduction of ZJR05 with P1 grown on EAW232
STL695AmpR &Tet repeatAB1157(89)
ZJR05AmpR &Tet repeat +ΔuupSTL695Transduction of STL695 with P1 grown on EAW242
StrainGenotypeParent strainSource/technique
MG1655uup+ radD+ xerD+–(125,126)
STL7180recA::catMG1655(92)
STL7212Δuup::FRT kanMG1655Lambda Red recombination
STL8027ΔradD::FRT catMG1655Lambda Red recombination
STL21852ΔradD::FRT catMG1655Transduction of MG1655 with P1 grown on STL8027
STL21884uup::FRT kanMG1655Transduction of MG1655 with P1 grown on STL7212
STL21912ΔradD::FRTMG1655CatS derivative of STL21852 after pCP20 transformation and curing
STL21712recA::catMG1655Transduction of MG1655 with P1 grown on STL7180
STL21936Δuup::FRT kan ΔradD::FRTMG1655Transduction of STL21912 with P1 grown on STL7212
STL21954recA::cat ΔradD::FRT catMG1655Transduction of STL21912 with P1 grown on STL7180
STL21932recA::cat Δuup::FRT kanMG1655Transduction of STL21884 with P1 grown on STL7180
STL21969recA::cat Δuup::FRT kan ΔradD::FRTMG1655Transduction of STL21936 with P1 grown on STL7180
ZJR01△uup°MG1655Transduction of MG1655 with P1 grown on EAW242
ZJR04△uup△radDMG1655Transduction of ZJR01 with P1 grown on EAW232
EAW526ΔradDMG1655Transduction of MG1655 with P1 grown on EAW232
EAW1133ΔxerDMG1655Lambda RED recombination
EAW1136ΔradD +Δlac IZYA +Δuup + ΔxerDEAW1132Transduction of EAW1132 with P1 grown on EAW1133
EAW1140AmpR &Tet repeat +ΔxerDSTL695Transduction of STL695 with P1 grown on EAW1133
EAW1145AmpR &Tet repeat +Δuup +ΔradD +ΔxerDEAW1139Transduction of EAW1139 with P1 grown on EAW1133
EAW242founder+Δe14+ΔuupMG1655Lambda RED recombination
EAW232founder+Δe14+ΔradDMG1655Lambda RED recombination
EAW1132ΔradD +Δlac IZYA +ΔuupEAW1100Transduction of EAW1100 with P1 grown on EAW242
EAW1100ΔradD +Δlac IZYAEAW526Transduction of EAW526 with P1 grown on EAW408
EAW408Δlac IZYAMG1655Lambda RED recombination
EAW1139AmpR &Tet repeat +Δuup +ΔradDZJR05Transduction of ZJR05 with P1 grown on EAW232
STL695AmpR &Tet repeatAB1157(89)
ZJR05AmpR &Tet repeat +ΔuupSTL695Transduction of STL695 with P1 grown on EAW242
Close
This Feature Is Available To Subscribers Only

Sign In or Create an Account

Close

This PDF is available to Subscribers Only

View Article Abstract & Purchase Options

For full access to this pdf, sign in to an existing account, or purchase an annual subscription.

Close