Table 1. Open in new tab Summary of the...

Environment

Temperature^a

Detection methods

Maximum methane oxidation rate

Methanotrophic genera identified

Reference/accession

Hydrothermal sediment, Guaymas Basin

5–85°C

Methane oxidation (anaerobic)

25 pmol L⁻¹ d⁻¹

Kallmeyer and Boetius 2004

Hydrothermal vent, East Pacific Rise

50–100°C

pmoA PCR

Methylothermus

Nercessian et al. 2005

Compost, Germany

25–70°C

Methane oxidation, cultivation

98.4 µmol g⁻¹ d⁻¹

Methylocaldum

Jäckel, Thummes and Kämpfer 2005

Compost, Belgium

10–66°C

16S rRNA gene qPCR with type I and type II specific primers

Type I methanotrophs (Gammaproteobacteria)

Halet, Boon and Verstraete 2006

Hydrothermal sediment, Mid Atlantic Ridge

44–91°C

Metagenomics (16S rRNA gene) (EMP)

Crenothrix, uncultured Methylococcaceae

Brazelton et al. 2010, ERP016395

Geothermal gas vents, Greece

32–39°C

Methane oxidation

11.4 nmol g⁻¹ d⁻¹

D'Alessandro et al. 2011

Hydrothermal sediment, Guaymas Basin

4–70, 50°C

Methane oxidation (anaerobic), 16S rRNA gene and mcrA PCR

1.2 µmol g⁻¹ d⁻¹

ANME-1, ANME-2c

Holler, Widdel and Knittel 2011

Geothermal soil, New Zealand

37–65°C

Methane oxidation, DNA-SIP

7.0 µmol g⁻¹ d⁻¹

Methylacidiphilum

Sharp, Stott and Dunfield 2012

Hot springs, Kamchatka, Russia

47–65°C

pmoA and 16S rRNA gene qPCR

Methylobacter, Methylomonas, Methylothermus

Kizilova et al. 2012

Hydrothermal vent, Taiwan

49.5°C

Metagenomics (MG-RAST)

Methylomarinum, Methylomicrobium

Meyer et al. 2008, MGP19574

Geothermal soil, Italy

33–83, 37–80°C

pmoA PCR, methane oxidation

2.4 µmol g⁻¹ d⁻¹

Methylacidiphilum, Methylocaldum, Methylococcus, Methylocystis

Gagliano et al. 2014

Geothermal soil, New Zealand

35–82, 37–65°C

pmoA and 16S rRNA gene PCR, methane oxidation, DNA-SIP

20.4 µmol g⁻¹ d⁻¹

Methylacidimicrobium, Methylacidiphilum

Sharp et al. 2014b

Geothermal soil, Canada

22–45°C

Methane oxidation, DNA-SIP

99 µmol g⁻¹ d⁻¹

Methylocaldum, Methylocapsa

Sharp et al. 2014a

Hot springs, Kuril Islands, Russia

44–99, 40–75°C

pmoA and 16S rRNA gene qPCR, methane oxidation

104 µmol L⁻¹ d⁻¹

Methylobacter, Methylococcus, Methylothermus

Kizilova et al. 2014

Hydrothermal vents, Norwegian Sea

<90°C

Metagenomics, metatranscriptomics, metaproteomics

Methylobacter

Urich et al. 2014

Hot spring, Yellowstone National Park (YNP), USA

86°C

nifH qRT-PCR (nitrogen fixation)

Methylacidiphilum

Hamilton et al. 2014

Geothermal lake, YNP, USA

62–66°C

Metagenomics (pmoA), 16S rRNA gene PCR

Methylothermus

Inskeep et al. 2015

Imperial Geyser microbial mats, YNP, USA

64–75°C

Metagenomics (16S rRNA gene) (EMP)

Methylocaldum, Methylomonas

Thompson et al. 2017, ERP022167

Microbial mats, Rainbow Spring, YNP, USA

42–90°C

Assembled metagenomes (IMG/M)

Methylacidiphilum

Chen et al. 2019

Joseph's Coat hot spring, YNP, USA

Assembled metagenomes (IMG/M)

Methylacidiphilum

Chen et al. 2019

Microbial mat, Mushroom Spring, YNP, USA

42–90°C

Assembled metagenomes (IMG/M)

Methylothermus

Chen et al. 2019

Microbial mat, hot spring, YNP, USA

42–90°C

Assembled metagenomes (IMG/M)

Methylococcus, Methylocystis, Methylosinus

Chen et al. 2019

Hot spring sediment, YNP, USA

42–90°C

Assembled metagenomes (IMG/M)

Methylacidiphilum

Chen et al. 2019

Environment

Temperature^a

Detection methods

Maximum methane oxidation rate

Methanotrophic genera identified

Reference/accession

Hydrothermal sediment, Guaymas Basin

5–85°C

Methane oxidation (anaerobic)

25 pmol L⁻¹ d⁻¹

Kallmeyer and Boetius 2004

Hydrothermal vent, East Pacific Rise

50–100°C

pmoA PCR

Methylothermus

Nercessian et al. 2005

Compost, Germany

25–70°C

Methane oxidation, cultivation

98.4 µmol g⁻¹ d⁻¹

Methylocaldum

Jäckel, Thummes and Kämpfer 2005

Compost, Belgium

10–66°C

16S rRNA gene qPCR with type I and type II specific primers

Type I methanotrophs (Gammaproteobacteria)

Halet, Boon and Verstraete 2006

Hydrothermal sediment, Mid Atlantic Ridge

44–91°C

Metagenomics (16S rRNA gene) (EMP)

Crenothrix, uncultured Methylococcaceae

Brazelton et al. 2010, ERP016395

Geothermal gas vents, Greece

32–39°C

Methane oxidation

11.4 nmol g⁻¹ d⁻¹

D'Alessandro et al. 2011

Hydrothermal sediment, Guaymas Basin

4–70, 50°C

Methane oxidation (anaerobic), 16S rRNA gene and mcrA PCR

1.2 µmol g⁻¹ d⁻¹

ANME-1, ANME-2c

Holler, Widdel and Knittel 2011

Geothermal soil, New Zealand

37–65°C

Methane oxidation, DNA-SIP

7.0 µmol g⁻¹ d⁻¹

Methylacidiphilum

Sharp, Stott and Dunfield 2012

Hot springs, Kamchatka, Russia

47–65°C

pmoA and 16S rRNA gene qPCR

Methylobacter, Methylomonas, Methylothermus

Kizilova et al. 2012

Hydrothermal vent, Taiwan

49.5°C

Metagenomics (MG-RAST)

Methylomarinum, Methylomicrobium

Meyer et al. 2008, MGP19574

Geothermal soil, Italy

33–83, 37–80°C

pmoA PCR, methane oxidation

2.4 µmol g⁻¹ d⁻¹

Methylacidiphilum, Methylocaldum, Methylococcus, Methylocystis

Gagliano et al. 2014

Geothermal soil, New Zealand

35–82, 37–65°C

pmoA and 16S rRNA gene PCR, methane oxidation, DNA-SIP

20.4 µmol g⁻¹ d⁻¹

Methylacidimicrobium, Methylacidiphilum

Sharp et al. 2014b

Geothermal soil, Canada

22–45°C

Methane oxidation, DNA-SIP

99 µmol g⁻¹ d⁻¹

Methylocaldum, Methylocapsa

Sharp et al. 2014a

Hot springs, Kuril Islands, Russia

44–99, 40–75°C

pmoA and 16S rRNA gene qPCR, methane oxidation

104 µmol L⁻¹ d⁻¹

Methylobacter, Methylococcus, Methylothermus

Kizilova et al. 2014

Hydrothermal vents, Norwegian Sea

<90°C

Metagenomics, metatranscriptomics, metaproteomics

Methylobacter

Urich et al. 2014

Hot spring, Yellowstone National Park (YNP), USA

86°C

nifH qRT-PCR (nitrogen fixation)

Methylacidiphilum

Hamilton et al. 2014

Geothermal lake, YNP, USA

62–66°C

Metagenomics (pmoA), 16S rRNA gene PCR

Methylothermus

Inskeep et al. 2015

Imperial Geyser microbial mats, YNP, USA

64–75°C

Metagenomics (16S rRNA gene) (EMP)

Methylocaldum, Methylomonas

Thompson et al. 2017, ERP022167

Microbial mats, Rainbow Spring, YNP, USA

42–90°C

Assembled metagenomes (IMG/M)

Methylacidiphilum

Chen et al. 2019

Joseph's Coat hot spring, YNP, USA

Assembled metagenomes (IMG/M)

Methylacidiphilum

Chen et al. 2019

Microbial mat, Mushroom Spring, YNP, USA

42–90°C

Assembled metagenomes (IMG/M)

Methylothermus

Chen et al. 2019

Microbial mat, hot spring, YNP, USA

42–90°C

Assembled metagenomes (IMG/M)

Methylococcus, Methylocystis, Methylosinus

Chen et al. 2019

Hot spring sediment, YNP, USA

42–90°C

Assembled metagenomes (IMG/M)

Methylacidiphilum

Chen et al. 2019

Table 1.

Open in new tab

Summary of the thermophilic methanotroph surveys discussed in this review.

Environment	Temperature^a	Detection methods	Maximum methane oxidation rate	Methanotrophic genera identified	Reference/accession
Hydrothermal sediment, Guaymas Basin	5–85°C	Methane oxidation (anaerobic)	25 pmol L⁻¹ d⁻¹	ND	Kallmeyer and Boetius 2004
Hydrothermal vent, East Pacific Rise	50–100°C	pmoA PCR	ND	Methylothermus	Nercessian et al. 2005
Compost, Germany	25–70°C	Methane oxidation, cultivation	98.4 µmol g⁻¹ d⁻¹	Methylocaldum	Jäckel, Thummes and Kämpfer 2005
Compost, Belgium	10–66°C	16S rRNA gene qPCR with type I and type II specific primers	ND	Type I methanotrophs (Gammaproteobacteria)	Halet, Boon and Verstraete 2006
Hydrothermal sediment, Mid Atlantic Ridge	44–91°C	Metagenomics (16S rRNA gene) (EMP)	ND	Crenothrix, uncultured Methylococcaceae	Brazelton et al. 2010, ERP016395
Geothermal gas vents, Greece	32–39°C	Methane oxidation	11.4 nmol g⁻¹ d⁻¹	ND	D'Alessandro et al. 2011
Hydrothermal sediment, Guaymas Basin	4–70, 50°C	Methane oxidation (anaerobic), 16S rRNA gene and mcrA PCR	1.2 µmol g⁻¹ d⁻¹	ANME-1, ANME-2c	Holler, Widdel and Knittel 2011
Geothermal soil, New Zealand	37–65°C	Methane oxidation, DNA-SIP	7.0 µmol g⁻¹ d⁻¹	Methylacidiphilum	Sharp, Stott and Dunfield 2012
Hot springs, Kamchatka, Russia	47–65°C	pmoA and 16S rRNA gene qPCR	ND	Methylobacter, Methylomonas, Methylothermus	Kizilova et al. 2012
Hydrothermal vent, Taiwan	49.5°C	Metagenomics (MG-RAST)	ND	Methylomarinum, Methylomicrobium	Meyer et al. 2008, MGP19574
Geothermal soil, Italy	33–83, 37–80°C	pmoA PCR, methane oxidation	2.4 µmol g⁻¹ d⁻¹	Methylacidiphilum, Methylocaldum, Methylococcus, Methylocystis	Gagliano et al. 2014
Geothermal soil, New Zealand	35–82, 37–65°C	pmoA and 16S rRNA gene PCR, methane oxidation, DNA-SIP	20.4 µmol g⁻¹ d⁻¹	Methylacidimicrobium, Methylacidiphilum	Sharp et al. 2014b
Geothermal soil, Canada	22–45°C	Methane oxidation, DNA-SIP	99 µmol g⁻¹ d⁻¹	Methylocaldum, Methylocapsa	Sharp et al. 2014a
Hot springs, Kuril Islands, Russia	44–99, 40–75°C	pmoA and 16S rRNA gene qPCR, methane oxidation	104 µmol L⁻¹ d⁻¹	Methylobacter, Methylococcus, Methylothermus	Kizilova et al. 2014
Hydrothermal vents, Norwegian Sea	<90°C	Metagenomics, metatranscriptomics, metaproteomics	ND	Methylobacter	Urich et al. 2014
Hot spring, Yellowstone National Park (YNP), USA	86°C	nifH qRT-PCR (nitrogen fixation)	ND	Methylacidiphilum	Hamilton et al. 2014
Geothermal lake, YNP, USA	62–66°C	Metagenomics (pmoA), 16S rRNA gene PCR	ND	Methylothermus	Inskeep et al. 2015
Imperial Geyser microbial mats, YNP, USA	64–75°C	Metagenomics (16S rRNA gene) (EMP)	ND	Methylocaldum, Methylomonas	Thompson et al. 2017, ERP022167
Microbial mats, Rainbow Spring, YNP, USA	42–90°C	Assembled metagenomes (IMG/M)	ND	Methylacidiphilum	Chen et al. 2019
Joseph's Coat hot spring, YNP, USA	ND	Assembled metagenomes (IMG/M)	ND	Methylacidiphilum	Chen et al. 2019
Microbial mat, Mushroom Spring, YNP, USA	42–90°C	Assembled metagenomes (IMG/M)	ND	Methylothermus	Chen et al. 2019
Microbial mat, hot spring, YNP, USA	42–90°C	Assembled metagenomes (IMG/M)	ND	Methylococcus, Methylocystis, Methylosinus	Chen et al. 2019
Hot spring sediment, YNP, USA	42–90°C	Assembled metagenomes (IMG/M)	ND	Methylacidiphilum	Chen et al. 2019

Environment	Temperature^a	Detection methods	Maximum methane oxidation rate	Methanotrophic genera identified	Reference/accession
Hydrothermal sediment, Guaymas Basin	5–85°C	Methane oxidation (anaerobic)	25 pmol L⁻¹ d⁻¹	ND	Kallmeyer and Boetius 2004
Hydrothermal vent, East Pacific Rise	50–100°C	pmoA PCR	ND	Methylothermus	Nercessian et al. 2005
Compost, Germany	25–70°C	Methane oxidation, cultivation	98.4 µmol g⁻¹ d⁻¹	Methylocaldum	Jäckel, Thummes and Kämpfer 2005
Compost, Belgium	10–66°C	16S rRNA gene qPCR with type I and type II specific primers	ND	Type I methanotrophs (Gammaproteobacteria)	Halet, Boon and Verstraete 2006
Hydrothermal sediment, Mid Atlantic Ridge	44–91°C	Metagenomics (16S rRNA gene) (EMP)	ND	Crenothrix, uncultured Methylococcaceae	Brazelton et al. 2010, ERP016395
Geothermal gas vents, Greece	32–39°C	Methane oxidation	11.4 nmol g⁻¹ d⁻¹	ND	D'Alessandro et al. 2011
Hydrothermal sediment, Guaymas Basin	4–70, 50°C	Methane oxidation (anaerobic), 16S rRNA gene and mcrA PCR	1.2 µmol g⁻¹ d⁻¹	ANME-1, ANME-2c	Holler, Widdel and Knittel 2011
Geothermal soil, New Zealand	37–65°C	Methane oxidation, DNA-SIP	7.0 µmol g⁻¹ d⁻¹	Methylacidiphilum	Sharp, Stott and Dunfield 2012
Hot springs, Kamchatka, Russia	47–65°C	pmoA and 16S rRNA gene qPCR	ND	Methylobacter, Methylomonas, Methylothermus	Kizilova et al. 2012
Hydrothermal vent, Taiwan	49.5°C	Metagenomics (MG-RAST)	ND	Methylomarinum, Methylomicrobium	Meyer et al. 2008, MGP19574
Geothermal soil, Italy	33–83, 37–80°C	pmoA PCR, methane oxidation	2.4 µmol g⁻¹ d⁻¹	Methylacidiphilum, Methylocaldum, Methylococcus, Methylocystis	Gagliano et al. 2014
Geothermal soil, New Zealand	35–82, 37–65°C	pmoA and 16S rRNA gene PCR, methane oxidation, DNA-SIP	20.4 µmol g⁻¹ d⁻¹	Methylacidimicrobium, Methylacidiphilum	Sharp et al. 2014b
Geothermal soil, Canada	22–45°C	Methane oxidation, DNA-SIP	99 µmol g⁻¹ d⁻¹	Methylocaldum, Methylocapsa	Sharp et al. 2014a
Hot springs, Kuril Islands, Russia	44–99, 40–75°C	pmoA and 16S rRNA gene qPCR, methane oxidation	104 µmol L⁻¹ d⁻¹	Methylobacter, Methylococcus, Methylothermus	Kizilova et al. 2014
Hydrothermal vents, Norwegian Sea	<90°C	Metagenomics, metatranscriptomics, metaproteomics	ND	Methylobacter	Urich et al. 2014
Hot spring, Yellowstone National Park (YNP), USA	86°C	nifH qRT-PCR (nitrogen fixation)	ND	Methylacidiphilum	Hamilton et al. 2014
Geothermal lake, YNP, USA	62–66°C	Metagenomics (pmoA), 16S rRNA gene PCR	ND	Methylothermus	Inskeep et al. 2015
Imperial Geyser microbial mats, YNP, USA	64–75°C	Metagenomics (16S rRNA gene) (EMP)	ND	Methylocaldum, Methylomonas	Thompson et al. 2017, ERP022167
Microbial mats, Rainbow Spring, YNP, USA	42–90°C	Assembled metagenomes (IMG/M)	ND	Methylacidiphilum	Chen et al. 2019
Joseph's Coat hot spring, YNP, USA	ND	Assembled metagenomes (IMG/M)	ND	Methylacidiphilum	Chen et al. 2019
Microbial mat, Mushroom Spring, YNP, USA	42–90°C	Assembled metagenomes (IMG/M)	ND	Methylothermus	Chen et al. 2019
Microbial mat, hot spring, YNP, USA	42–90°C	Assembled metagenomes (IMG/M)	ND	Methylococcus, Methylocystis, Methylosinus	Chen et al. 2019
Hot spring sediment, YNP, USA	42–90°C	Assembled metagenomes (IMG/M)	ND	Methylacidiphilum	Chen et al. 2019

In situ temperature shown for molecular detection methods, and incubation temperatures for methane oxidation rates. ND, not determined; EMP, Earth Microbiome Project; MG-RAST, Metagenomic Rapid Annotations using Subsystems Technology; IMG/M, Integrated Microbial Genomes and Microbiomes.

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