Table 2.
Open in new tab

Mutations and amino acid substitutions identified in oxacillin-resistant isolates from Austria (n = 10) and the UK (n = 11)

CountryIsolateMLST STERA accessionPBP1PBP2PBP3pbp4 promoterPBP4AcrBYjbHGdpP
Austria51161ERR599830−−−−R200L−−R397*
32777ERR599821−Q629P−−C12F, T101R−−Q572*
155615ERR599824M361TS569A–G>A at −39 bp−−M262I−
272522ERR599822E606K, S629T, S664T−R504K, K584NG>T at −62 bp, C>T at −298 bp, C>T at 407 bpD98EA284S, M291V, K846E−V417*
544122ERR599823S629T, S644T−R504K, K584NG>T at −62 bp, C>T at −298 bp, C>T at 407 bpD98EA284S, M291V, K846E−S207*
329822ERR599826E606K, S629T, S664T−R504K, K584NG>T at −62 bp, C>T at −298 bp, C>T at 407 bpD98EA284S, M291V, K846E−V417*
395522ERR599825S629T, S644T−R504K, K584NG>T at −62 bp, C>T at −298 bp, C>T at 407 bpD98EA284S, M291V, K846E−E334*
461325ERR599829D149ER117H, Q629PK6N−−K35N, F581L, V700LS267L−
332245ERR599831−A470T−G>T at −62 bp−V138I−S50*
3397225ERR599832−S707L−−−−−V609F
UKm-30-768ERR211695−−−−−−−Q642*
m-36-288ERR234780P100T, P476S−−A>C at 117 bp−−−K411*
m-35-3897ERR234716−−G374D,A596T−−−−Q480*
m-MR-11G97ERR175884−−−−−−−Q101*
m-MR-11Z97ERR175932−−−−−−−A639*
m-MR-089A97ERR175934−−−−−−−T104K, E423*
m-30-4133ERR211688−−−−−−−542–546 deletion
m-30-64133ERR211693−−−−A22V−A242SE267*
m-36-35133ERR234788−V233I−−−−−R382*
m-29-22425ERR211684−−−−−−−I233*
m-30-43425ERR211692−−−−−−−R540*
CountryIsolateMLST STERA accessionPBP1PBP2PBP3pbp4 promoterPBP4AcrBYjbHGdpP
Austria51161ERR599830−−−−R200L−−R397*
32777ERR599821−Q629P−−C12F, T101R−−Q572*
155615ERR599824M361TS569A–G>A at −39 bp−−M262I−
272522ERR599822E606K, S629T, S664T−R504K, K584NG>T at −62 bp, C>T at −298 bp, C>T at 407 bpD98EA284S, M291V, K846E−V417*
544122ERR599823S629T, S644T−R504K, K584NG>T at −62 bp, C>T at −298 bp, C>T at 407 bpD98EA284S, M291V, K846E−S207*
329822ERR599826E606K, S629T, S664T−R504K, K584NG>T at −62 bp, C>T at −298 bp, C>T at 407 bpD98EA284S, M291V, K846E−V417*
395522ERR599825S629T, S644T−R504K, K584NG>T at −62 bp, C>T at −298 bp, C>T at 407 bpD98EA284S, M291V, K846E−E334*
461325ERR599829D149ER117H, Q629PK6N−−K35N, F581L, V700LS267L−
332245ERR599831−A470T−G>T at −62 bp−V138I−S50*
3397225ERR599832−S707L−−−−−V609F
UKm-30-768ERR211695−−−−−−−Q642*
m-36-288ERR234780P100T, P476S−−A>C at 117 bp−−−K411*
m-35-3897ERR234716−−G374D,A596T−−−−Q480*
m-MR-11G97ERR175884−−−−−−−Q101*
m-MR-11Z97ERR175932−−−−−−−A639*
m-MR-089A97ERR175934−−−−−−−T104K, E423*
m-30-4133ERR211688−−−−−−−542–546 deletion
m-30-64133ERR211693−−−−A22V−A242SE267*
m-36-35133ERR234788−V233I−−−−−R382*
m-29-22425ERR211684−−−−−−−I233*
m-30-43425ERR211692−−−−−−−R540*
*

, Stop codon; −, absence of mutations or amino acid substitutions.

Novel amino acid substitutions are in bold and amino acid substitutions in transpeptidase domains of PBPs are in italics.

Mutations and amino acid substitutions listed in the table were not present in the MSSA isolates used as controls. MSSAs were five ST1 isolates (ERR237623, ERR246646, ERR246644, ERR246656 and ERR234851), five ST97 isolates (ERR234740, ERR175922, ERR175911, ERR175906 and ERR175903), five ST425 isolates (ERR211673, ERR211668, ERR246593, ERR084608 and ERR211685), four ST133 isolates (ERR211694, ERR246594, ERR246606 and ERR237612), two ST8 isolates (ERR294352 and ERR144823) and two ST15 isolates (ERR234888 and ERR234842).

Table 2.
Open in new tab

Mutations and amino acid substitutions identified in oxacillin-resistant isolates from Austria (n = 10) and the UK (n = 11)

CountryIsolateMLST STERA accessionPBP1PBP2PBP3pbp4 promoterPBP4AcrBYjbHGdpP
Austria51161ERR599830−−−−R200L−−R397*
32777ERR599821−Q629P−−C12F, T101R−−Q572*
155615ERR599824M361TS569A–G>A at −39 bp−−M262I−
272522ERR599822E606K, S629T, S664T−R504K, K584NG>T at −62 bp, C>T at −298 bp, C>T at 407 bpD98EA284S, M291V, K846E−V417*
544122ERR599823S629T, S644T−R504K, K584NG>T at −62 bp, C>T at −298 bp, C>T at 407 bpD98EA284S, M291V, K846E−S207*
329822ERR599826E606K, S629T, S664T−R504K, K584NG>T at −62 bp, C>T at −298 bp, C>T at 407 bpD98EA284S, M291V, K846E−V417*
395522ERR599825S629T, S644T−R504K, K584NG>T at −62 bp, C>T at −298 bp, C>T at 407 bpD98EA284S, M291V, K846E−E334*
461325ERR599829D149ER117H, Q629PK6N−−K35N, F581L, V700LS267L−
332245ERR599831−A470T−G>T at −62 bp−V138I−S50*
3397225ERR599832−S707L−−−−−V609F
UKm-30-768ERR211695−−−−−−−Q642*
m-36-288ERR234780P100T, P476S−−A>C at 117 bp−−−K411*
m-35-3897ERR234716−−G374D,A596T−−−−Q480*
m-MR-11G97ERR175884−−−−−−−Q101*
m-MR-11Z97ERR175932−−−−−−−A639*
m-MR-089A97ERR175934−−−−−−−T104K, E423*
m-30-4133ERR211688−−−−−−−542–546 deletion
m-30-64133ERR211693−−−−A22V−A242SE267*
m-36-35133ERR234788−V233I−−−−−R382*
m-29-22425ERR211684−−−−−−−I233*
m-30-43425ERR211692−−−−−−−R540*
CountryIsolateMLST STERA accessionPBP1PBP2PBP3pbp4 promoterPBP4AcrBYjbHGdpP
Austria51161ERR599830−−−−R200L−−R397*
32777ERR599821−Q629P−−C12F, T101R−−Q572*
155615ERR599824M361TS569A–G>A at −39 bp−−M262I−
272522ERR599822E606K, S629T, S664T−R504K, K584NG>T at −62 bp, C>T at −298 bp, C>T at 407 bpD98EA284S, M291V, K846E−V417*
544122ERR599823S629T, S644T−R504K, K584NG>T at −62 bp, C>T at −298 bp, C>T at 407 bpD98EA284S, M291V, K846E−S207*
329822ERR599826E606K, S629T, S664T−R504K, K584NG>T at −62 bp, C>T at −298 bp, C>T at 407 bpD98EA284S, M291V, K846E−V417*
395522ERR599825S629T, S644T−R504K, K584NG>T at −62 bp, C>T at −298 bp, C>T at 407 bpD98EA284S, M291V, K846E−E334*
461325ERR599829D149ER117H, Q629PK6N−−K35N, F581L, V700LS267L−
332245ERR599831−A470T−G>T at −62 bp−V138I−S50*
3397225ERR599832−S707L−−−−−V609F
UKm-30-768ERR211695−−−−−−−Q642*
m-36-288ERR234780P100T, P476S−−A>C at 117 bp−−−K411*
m-35-3897ERR234716−−G374D,A596T−−−−Q480*
m-MR-11G97ERR175884−−−−−−−Q101*
m-MR-11Z97ERR175932−−−−−−−A639*
m-MR-089A97ERR175934−−−−−−−T104K, E423*
m-30-4133ERR211688−−−−−−−542–546 deletion
m-30-64133ERR211693−−−−A22V−A242SE267*
m-36-35133ERR234788−V233I−−−−−R382*
m-29-22425ERR211684−−−−−−−I233*
m-30-43425ERR211692−−−−−−−R540*
*

, Stop codon; −, absence of mutations or amino acid substitutions.

Novel amino acid substitutions are in bold and amino acid substitutions in transpeptidase domains of PBPs are in italics.

Mutations and amino acid substitutions listed in the table were not present in the MSSA isolates used as controls. MSSAs were five ST1 isolates (ERR237623, ERR246646, ERR246644, ERR246656 and ERR234851), five ST97 isolates (ERR234740, ERR175922, ERR175911, ERR175906 and ERR175903), five ST425 isolates (ERR211673, ERR211668, ERR246593, ERR084608 and ERR211685), four ST133 isolates (ERR211694, ERR246594, ERR246606 and ERR237612), two ST8 isolates (ERR294352 and ERR144823) and two ST15 isolates (ERR234888 and ERR234842).

Close
This Feature Is Available To Subscribers Only

Sign In or Create an Account

Close

This PDF is available to Subscribers Only

View Article Abstract & Purchase Options

For full access to this pdf, sign in to an existing account, or purchase an annual subscription.

Close