Standardization of different parameters to achieve optimum growth for MALDI-TOF MS-based resistance detection
| . | Growth medium . | |
|---|---|---|
| Characteristics . | DMEM . | RPMI . |
| Cell concentration (cells/mL) | ||
| 1 × 106 | no detectable growth | no detectable growth |
| 5 × 106 | detectable growth | detectable growth |
| 1 × 107 | detectable growth | detectable growth |
| 1 × 108 | detectable growth | detectable growth |
| Drug concentration (mg/L) | ||
| 16 | growth | growth |
| 80 | reduced growth | reduced growth |
| 160 | growth inhibition of susceptible isolates | growth inhibition of susceptible isolates |
| Media components (g/L)a | ||
| G = 4; M = 0.30; L = 0.1 | no growth | no growth |
| G = 4; M = 0.60; L = 0.2 | slow growth | slow growth |
| G = 8; M = 0.30; L = 0.1 | slow growth | slow growth |
| G = 8; M = 0.60; L = 0.2 | efficient growth | efficient growth |
| G = 8; M = 0.12; L = 0.4 | efficient growth | efficient growth |
| G = 8; M = 0.24; L = 0.8 | efficient growth | efficient growth |
| . | Growth medium . | |
|---|---|---|
| Characteristics . | DMEM . | RPMI . |
| Cell concentration (cells/mL) | ||
| 1 × 106 | no detectable growth | no detectable growth |
| 5 × 106 | detectable growth | detectable growth |
| 1 × 107 | detectable growth | detectable growth |
| 1 × 108 | detectable growth | detectable growth |
| Drug concentration (mg/L) | ||
| 16 | growth | growth |
| 80 | reduced growth | reduced growth |
| 160 | growth inhibition of susceptible isolates | growth inhibition of susceptible isolates |
| Media components (g/L)a | ||
| G = 4; M = 0.30; L = 0.1 | no growth | no growth |
| G = 4; M = 0.60; L = 0.2 | slow growth | slow growth |
| G = 8; M = 0.30; L = 0.1 | slow growth | slow growth |
| G = 8; M = 0.60; L = 0.2 | efficient growth | efficient growth |
| G = 8; M = 0.12; L = 0.4 | efficient growth | efficient growth |
| G = 8; M = 0.24; L = 0.8 | efficient growth | efficient growth |
G, glucose; M, yeast synthetic drop-out medium supplements; L, lysine.
Standardization of different parameters to achieve optimum growth for MALDI-TOF MS-based resistance detection
| . | Growth medium . | |
|---|---|---|
| Characteristics . | DMEM . | RPMI . |
| Cell concentration (cells/mL) | ||
| 1 × 106 | no detectable growth | no detectable growth |
| 5 × 106 | detectable growth | detectable growth |
| 1 × 107 | detectable growth | detectable growth |
| 1 × 108 | detectable growth | detectable growth |
| Drug concentration (mg/L) | ||
| 16 | growth | growth |
| 80 | reduced growth | reduced growth |
| 160 | growth inhibition of susceptible isolates | growth inhibition of susceptible isolates |
| Media components (g/L)a | ||
| G = 4; M = 0.30; L = 0.1 | no growth | no growth |
| G = 4; M = 0.60; L = 0.2 | slow growth | slow growth |
| G = 8; M = 0.30; L = 0.1 | slow growth | slow growth |
| G = 8; M = 0.60; L = 0.2 | efficient growth | efficient growth |
| G = 8; M = 0.12; L = 0.4 | efficient growth | efficient growth |
| G = 8; M = 0.24; L = 0.8 | efficient growth | efficient growth |
| . | Growth medium . | |
|---|---|---|
| Characteristics . | DMEM . | RPMI . |
| Cell concentration (cells/mL) | ||
| 1 × 106 | no detectable growth | no detectable growth |
| 5 × 106 | detectable growth | detectable growth |
| 1 × 107 | detectable growth | detectable growth |
| 1 × 108 | detectable growth | detectable growth |
| Drug concentration (mg/L) | ||
| 16 | growth | growth |
| 80 | reduced growth | reduced growth |
| 160 | growth inhibition of susceptible isolates | growth inhibition of susceptible isolates |
| Media components (g/L)a | ||
| G = 4; M = 0.30; L = 0.1 | no growth | no growth |
| G = 4; M = 0.60; L = 0.2 | slow growth | slow growth |
| G = 8; M = 0.30; L = 0.1 | slow growth | slow growth |
| G = 8; M = 0.60; L = 0.2 | efficient growth | efficient growth |
| G = 8; M = 0.12; L = 0.4 | efficient growth | efficient growth |
| G = 8; M = 0.24; L = 0.8 | efficient growth | efficient growth |
G, glucose; M, yeast synthetic drop-out medium supplements; L, lysine.
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