Table 1.

Grafting success (expressed as percentage of all grafts performed) between pea (Pisum sativum L.) plant hormone mutants and the parental wild-type line

Graft type (scion/rootstock)
HormoneGeneActionWT/WTMutant/MutantMutant/WTWT/Mutantn
Gibberellin biosynthesisNAent-Kaurenoic acid oxidase67 %88 %63 %67 %24
LEGibberellin 3-oxidase67 %58 %67 %42 %12
LHent-Kaurene oxidase71 %83 %56 %75 %*
Brassinosteroid biosynthesisLKSteroidal 5α-reductase60 %40 %40 %73 %15
Strigolactone biosynthesisCCD8Carotenoid cleavage dioxygenase 844 %50 %38 %38 %16
Strigolactone responseMAX2F-box protein45 %35 %50 %45 %20
Ethylene responseEIN2N-RAMP metal transporter-like protein63 %44 %57 %nd
Auxin responseRMS2Putative auxin receptor AFDB4/540 %50 %35 %45 %20
Auxin deficientBSHUnknown93 %93 %93 %80 %15
Graft type (scion/rootstock)
HormoneGeneActionWT/WTMutant/MutantMutant/WTWT/Mutantn
Gibberellin biosynthesisNAent-Kaurenoic acid oxidase67 %88 %63 %67 %24
LEGibberellin 3-oxidase67 %58 %67 %42 %12
LHent-Kaurene oxidase71 %83 %56 %75 %*
Brassinosteroid biosynthesisLKSteroidal 5α-reductase60 %40 %40 %73 %15
Strigolactone biosynthesisCCD8Carotenoid cleavage dioxygenase 844 %50 %38 %38 %16
Strigolactone responseMAX2F-box protein45 %35 %50 %45 %20
Ethylene responseEIN2N-RAMP metal transporter-like protein63 %44 %57 %nd
Auxin responseRMS2Putative auxin receptor AFDB4/540 %50 %35 %45 %20
Auxin deficientBSHUnknown93 %93 %93 %80 %15

Successful grafts are defined as those in which the scion developed through to flowering with active growth of the main stem. Results are mostly from epicotyl/epicotyl grafts, but in some instances involved leafy shoots and are aggregated across more than one experiment. The number of grafts attempted is shown (n). No significant differences in grafting success were observed between graft types for any mutant when tested by contingency χ2. The results are from grafts done over many years including those done for other purposes reported in Reid et al. (1983), Reid and Ross (1989), Beveridge et al. (1996) and Symons et al. (1999) and unpublished results for ein2.

*n = 24 grafts for the self-grafts and 12 for the Mutant/WT and WT/Mutant grafts.

n = 24 grafts for WT/WT, 16 for Mutant/Mutant and 28 for Mutant/WT.

nd = not done.

Table 1.

Grafting success (expressed as percentage of all grafts performed) between pea (Pisum sativum L.) plant hormone mutants and the parental wild-type line

Graft type (scion/rootstock)
HormoneGeneActionWT/WTMutant/MutantMutant/WTWT/Mutantn
Gibberellin biosynthesisNAent-Kaurenoic acid oxidase67 %88 %63 %67 %24
LEGibberellin 3-oxidase67 %58 %67 %42 %12
LHent-Kaurene oxidase71 %83 %56 %75 %*
Brassinosteroid biosynthesisLKSteroidal 5α-reductase60 %40 %40 %73 %15
Strigolactone biosynthesisCCD8Carotenoid cleavage dioxygenase 844 %50 %38 %38 %16
Strigolactone responseMAX2F-box protein45 %35 %50 %45 %20
Ethylene responseEIN2N-RAMP metal transporter-like protein63 %44 %57 %nd
Auxin responseRMS2Putative auxin receptor AFDB4/540 %50 %35 %45 %20
Auxin deficientBSHUnknown93 %93 %93 %80 %15
Graft type (scion/rootstock)
HormoneGeneActionWT/WTMutant/MutantMutant/WTWT/Mutantn
Gibberellin biosynthesisNAent-Kaurenoic acid oxidase67 %88 %63 %67 %24
LEGibberellin 3-oxidase67 %58 %67 %42 %12
LHent-Kaurene oxidase71 %83 %56 %75 %*
Brassinosteroid biosynthesisLKSteroidal 5α-reductase60 %40 %40 %73 %15
Strigolactone biosynthesisCCD8Carotenoid cleavage dioxygenase 844 %50 %38 %38 %16
Strigolactone responseMAX2F-box protein45 %35 %50 %45 %20
Ethylene responseEIN2N-RAMP metal transporter-like protein63 %44 %57 %nd
Auxin responseRMS2Putative auxin receptor AFDB4/540 %50 %35 %45 %20
Auxin deficientBSHUnknown93 %93 %93 %80 %15

Successful grafts are defined as those in which the scion developed through to flowering with active growth of the main stem. Results are mostly from epicotyl/epicotyl grafts, but in some instances involved leafy shoots and are aggregated across more than one experiment. The number of grafts attempted is shown (n). No significant differences in grafting success were observed between graft types for any mutant when tested by contingency χ2. The results are from grafts done over many years including those done for other purposes reported in Reid et al. (1983), Reid and Ross (1989), Beveridge et al. (1996) and Symons et al. (1999) and unpublished results for ein2.

*n = 24 grafts for the self-grafts and 12 for the Mutant/WT and WT/Mutant grafts.

n = 24 grafts for WT/WT, 16 for Mutant/Mutant and 28 for Mutant/WT.

nd = not done.

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