TABLE 2 Open in new tab Rat Pulmonary...

	Total bronchoalveolar lavage fluid (BALF) protein and cellularity
Vehicle	560 ± 149	638 ± 100	5.7 ± 1.4	3.5 ± 0.9	5.3 ± 1.3	5.7 ± 0.7	14.2 ± 10	2.1 ± 0.3	1.9 ± 1.1	2.4 ± 2.4	6.6 ± 2.4	1.7 ± 1.7
C₆₀	316 ± 55	750 ± 138*	3.9 ± 0.8	6.9 ± 1.7	4.0 ± 0.7	2.5 ± 1.2	13.3 ± 12	1.7 ± 1.4	0.5 ± 0.5	<1	2.8 ± 0.3	8.2 ± 2.6
Naïve	456 ± 31	5.6 ± 0.5	5.6 ± 0.5	7.7 ± 4.2	<1	8.7 ± 4.0
n	4–5	4–6	3–5	3–5	3–5	3–5
*p < 0.05 versus IT C_60.
	Total bronchoalveolar lavage fluid (BALF) protein and cellularity
	BAL samples collected 24 h following exposure in females, data expressed as mean ± SEM
	Protein (μg/ml)	Cell number (× 10⁵)	Macrophages (× 10⁵)	Neutrophils (× 10³)	Eosinophils (× 10³)	Epithelial cells (× 10³)
	IT	IV	IT	IV	IT	IV	IT	IV	IT	IV	IT	IV
Vehicle	252 ± 28	195 ± 8.2	7.8 ± 1.9	4.1 ± 0.6†	1.6 ± 0.9	3.3 ± 0.4	35.0 ± 25.3	<1	0.25 ± 0.25*	1.7 ± 1.7	132 ± 90	346 ± 132
C₆₀	206 ± 24	226 ± 41	7.1 ± 1.2	3.5 ± 0.5*	0.9 ± 0.3	3.1 ± 1.1	27.5 ± 11.1	<1	12.5 ± 7.5	3.6 ± 0.6	75.0 ± 33	362 ± 59*
Naïve	N/A	6.6 ± 1.0	6.6 ± 1.1*,†	<1	<1	179 ± 43
n	5–6	4–6	4–6	4–6	4–6	4–6

Total bronchoalveolar lavage fluid (BALF) protein and cellularity

BAL samples collected 24 h following exposure in males, data expressed as mean ± SEM

Protein (μg/ml)

Cell number (× 10⁵)

Macrophages (× 10⁵)

Neutrophils (x 10³)

Eosinophils (×10³)

Epithelial cells (× 10³)

Vehicle

560 ± 149

638 ± 100

5.7 ± 1.4

3.5 ± 0.9

5.3 ± 1.3

5.7 ± 0.7

14.2 ± 10

2.1 ± 0.3

1.9 ± 1.1

2.4 ± 2.4

6.6 ± 2.4

1.7 ± 1.7

C₆₀

316 ± 55

750 ± 138*

3.9 ± 0.8

6.9 ± 1.7

4.0 ± 0.7

2.5 ± 1.2

13.3 ± 12

1.7 ± 1.4

0.5 ± 0.5

2.8 ± 0.3

8.2 ± 2.6

Naïve

456 ± 31

5.6 ± 0.5

7.7 ± 4.2

8.7 ± 4.0

4–5

4–6

3–5

*p < 0.05 versus IT C_60.

Total bronchoalveolar lavage fluid (BALF) protein and cellularity

BAL samples collected 24 h following exposure in females, data expressed as mean ± SEM

Protein (μg/ml)

Cell number (× 10⁵)

Macrophages (× 10⁵)

Neutrophils (× 10³)

Eosinophils (× 10³)

Epithelial cells (× 10³)

Vehicle

252 ± 28

195 ± 8.2

7.8 ± 1.9

4.1 ± 0.6†

1.6 ± 0.9

3.3 ± 0.4

35.0 ± 25.3

0.25 ± 0.25*

1.7 ± 1.7

132 ± 90

346 ± 132

C₆₀

206 ± 24

226 ± 41

7.1 ± 1.2

3.5 ± 0.5*

0.9 ± 0.3

3.1 ± 1.1

27.5 ± 11.1

12.5 ± 7.5

3.6 ± 0.6

75.0 ± 33

362 ± 59*

Naïve

N/A

6.6 ± 1.0

6.6 ± 1.1*,†

179 ± 43

5–6

4–6

	Total bronchoalveolar lavage fluid (BALF) protein and cellularity
Vehicle	560 ± 149	638 ± 100	5.7 ± 1.4	3.5 ± 0.9	5.3 ± 1.3	5.7 ± 0.7	14.2 ± 10	2.1 ± 0.3	1.9 ± 1.1	2.4 ± 2.4	6.6 ± 2.4	1.7 ± 1.7
C₆₀	316 ± 55	750 ± 138*	3.9 ± 0.8	6.9 ± 1.7	4.0 ± 0.7	2.5 ± 1.2	13.3 ± 12	1.7 ± 1.4	0.5 ± 0.5	<1	2.8 ± 0.3	8.2 ± 2.6
Naïve	456 ± 31	5.6 ± 0.5	5.6 ± 0.5	7.7 ± 4.2	<1	8.7 ± 4.0
n	4–5	4–6	3–5	3–5	3–5	3–5
*p < 0.05 versus IT C_60.
	Total bronchoalveolar lavage fluid (BALF) protein and cellularity
	BAL samples collected 24 h following exposure in females, data expressed as mean ± SEM
	Protein (μg/ml)	Cell number (× 10⁵)	Macrophages (× 10⁵)	Neutrophils (× 10³)	Eosinophils (× 10³)	Epithelial cells (× 10³)
	IT	IV	IT	IV	IT	IV	IT	IV	IT	IV	IT	IV
Vehicle	252 ± 28	195 ± 8.2	7.8 ± 1.9	4.1 ± 0.6†	1.6 ± 0.9	3.3 ± 0.4	35.0 ± 25.3	<1	0.25 ± 0.25*	1.7 ± 1.7	132 ± 90	346 ± 132
C₆₀	206 ± 24	226 ± 41	7.1 ± 1.2	3.5 ± 0.5*	0.9 ± 0.3	3.1 ± 1.1	27.5 ± 11.1	<1	12.5 ± 7.5	3.6 ± 0.6	75.0 ± 33	362 ± 59*
Naïve	N/A	6.6 ± 1.0	6.6 ± 1.1*,†	<1	<1	179 ± 43
n	5–6	4–6	4–6	4–6	4–6	4–6

TABLE 2

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Rat Pulmonary Responses to Intratracheal (IT) or Intravenous (IV) Delivery of C₆₀ or Vehicle Suspensions

	Total bronchoalveolar lavage fluid (BALF) protein and cellularity
	BAL samples collected 24 h following exposure in males, data expressed as mean ± SEM
	Protein (μg/ml)		Cell number (× 10⁵)		Macrophages (× 10⁵)		Neutrophils (x 10³)		Eosinophils (×10³)		Epithelial cells (× 10³)
	IT	IV	IT	IV	IT	IV	IT	IV	IT	IV	IT	IV
Vehicle	560 ± 149	638 ± 100	5.7 ± 1.4	3.5 ± 0.9	5.3 ± 1.3	5.7 ± 0.7	14.2 ± 10	2.1 ± 0.3	1.9 ± 1.1	2.4 ± 2.4	6.6 ± 2.4	1.7 ± 1.7
C₆₀	316 ± 55	750 ± 138*	3.9 ± 0.8	6.9 ± 1.7	4.0 ± 0.7	2.5 ± 1.2	13.3 ± 12	1.7 ± 1.4	0.5 ± 0.5	<1	2.8 ± 0.3	8.2 ± 2.6
Naïve	456 ± 31		5.6 ± 0.5		5.6 ± 0.5		7.7 ± 4.2		<1		8.7 ± 4.0
n	4–5		4–6		3–5		3–5		3–5		3–5
*p < 0.05 versus IT C_60.
	Total bronchoalveolar lavage fluid (BALF) protein and cellularity
	BAL samples collected 24 h following exposure in females, data expressed as mean ± SEM
	Protein (μg/ml)		Cell number (× 10⁵)		Macrophages (× 10⁵)		Neutrophils (× 10³)		Eosinophils (× 10³)		Epithelial cells (× 10³)
	IT	IV	IT	IV	IT	IV	IT	IV	IT	IV	IT	IV
Vehicle	252 ± 28	195 ± 8.2	7.8 ± 1.9	4.1 ± 0.6†	1.6 ± 0.9	3.3 ± 0.4	35.0 ± 25.3	<1	0.25 ± 0.25*	1.7 ± 1.7	132 ± 90	346 ± 132
C₆₀	206 ± 24	226 ± 41	7.1 ± 1.2	3.5 ± 0.5*	0.9 ± 0.3	3.1 ± 1.1	27.5 ± 11.1	<1	12.5 ± 7.5	3.6 ± 0.6	75.0 ± 33	362 ± 59*
Naïve	N/A		6.6 ± 1.0		6.6 ± 1.1*,†		<1		<1		179 ± 43
n	5–6		4–6		4–6		4–6		4–6		4–6

	Total bronchoalveolar lavage fluid (BALF) protein and cellularity
	BAL samples collected 24 h following exposure in males, data expressed as mean ± SEM
	Protein (μg/ml)		Cell number (× 10⁵)		Macrophages (× 10⁵)		Neutrophils (x 10³)		Eosinophils (×10³)		Epithelial cells (× 10³)
	IT	IV	IT	IV	IT	IV	IT	IV	IT	IV	IT	IV
Vehicle	560 ± 149	638 ± 100	5.7 ± 1.4	3.5 ± 0.9	5.3 ± 1.3	5.7 ± 0.7	14.2 ± 10	2.1 ± 0.3	1.9 ± 1.1	2.4 ± 2.4	6.6 ± 2.4	1.7 ± 1.7
C₆₀	316 ± 55	750 ± 138*	3.9 ± 0.8	6.9 ± 1.7	4.0 ± 0.7	2.5 ± 1.2	13.3 ± 12	1.7 ± 1.4	0.5 ± 0.5	<1	2.8 ± 0.3	8.2 ± 2.6
Naïve	456 ± 31		5.6 ± 0.5		5.6 ± 0.5		7.7 ± 4.2		<1		8.7 ± 4.0
n	4–5		4–6		3–5		3–5		3–5		3–5
*p < 0.05 versus IT C_60.
	Total bronchoalveolar lavage fluid (BALF) protein and cellularity
	BAL samples collected 24 h following exposure in females, data expressed as mean ± SEM
	Protein (μg/ml)		Cell number (× 10⁵)		Macrophages (× 10⁵)		Neutrophils (× 10³)		Eosinophils (× 10³)		Epithelial cells (× 10³)
	IT	IV	IT	IV	IT	IV	IT	IV	IT	IV	IT	IV
Vehicle	252 ± 28	195 ± 8.2	7.8 ± 1.9	4.1 ± 0.6†	1.6 ± 0.9	3.3 ± 0.4	35.0 ± 25.3	<1	0.25 ± 0.25*	1.7 ± 1.7	132 ± 90	346 ± 132
C₆₀	206 ± 24	226 ± 41	7.1 ± 1.2	3.5 ± 0.5*	0.9 ± 0.3	3.1 ± 1.1	27.5 ± 11.1	<1	12.5 ± 7.5	3.6 ± 0.6	75.0 ± 33	362 ± 59*
Naïve	N/A		6.6 ± 1.0		6.6 ± 1.1*,†		<1		<1		179 ± 43
n	5–6		4–6		4–6		4–6		4–6		4–6

N/A, not applicable.

*p < 0.05 versus IT C₆₀.

†p < 0.05 versus IT vehicle.

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