These q-value filtered datasets were loaded onto the IPA program in order to further characterize the molecular processes they may reflect. Consistent with previous observations, a preliminary characterization suggested an increase in organismal death and inflammation-related processes in the endometrial fluid of non-implantative women, as well as an inhibition of cell movement and migration-related terms (Table II, Supplementary File S5). Interestingly, both datasets reflected a link between the deregulated proteins and the PI3K/AKT signaling pathway, as well as with the transcription regulator nuclear factor, erythroid 2 like 2 (Nfe2l2, also named Nrf2) (Fig. 3). Based on the differential protein expression pattern, both signaling networks appear to be inhibited in the non-implantative EFA.

Table II

Summary of the results obtained for the discovery and validation datasets.

DiscoveryValidation
ProcessP-valueZ-scoreP-valueZ-score
Cell movement1.74E-03−2.035.50E-14−2.81
Cell migration1.01E-03−1.9874.01E-10−2.76
Organismal death3.46E-051.783.93E-062.983
Inflammation of organ1.5E_041.2017.13E-081.269
DiscoveryValidation
ProcessP-valueZ-scoreP-valueZ-score
Cell movement1.74E-03−2.035.50E-14−2.81
Cell migration1.01E-03−1.9874.01E-10−2.76
Organismal death3.46E-051.783.93E-062.983
Inflammation of organ1.5E_041.2017.13E-081.269

P-values determine the probability that the association between proteins in the dataset and a process is explained by chance alone. Z-score represents the bias in gene regulation that predicts whether the upstream regulator exists in an activated (positive values) or inactivated (negative values) state.

Table II

Summary of the results obtained for the discovery and validation datasets.

DiscoveryValidation
ProcessP-valueZ-scoreP-valueZ-score
Cell movement1.74E-03−2.035.50E-14−2.81
Cell migration1.01E-03−1.9874.01E-10−2.76
Organismal death3.46E-051.783.93E-062.983
Inflammation of organ1.5E_041.2017.13E-081.269
DiscoveryValidation
ProcessP-valueZ-scoreP-valueZ-score
Cell movement1.74E-03−2.035.50E-14−2.81
Cell migration1.01E-03−1.9874.01E-10−2.76
Organismal death3.46E-051.783.93E-062.983
Inflammation of organ1.5E_041.2017.13E-081.269

P-values determine the probability that the association between proteins in the dataset and a process is explained by chance alone. Z-score represents the bias in gene regulation that predicts whether the upstream regulator exists in an activated (positive values) or inactivated (negative values) state.

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