Characteristics of 9 Heart Transplant Recipients and 11 Patients in the Genotyping Control Group Infected with Pneumocystis jirovecii a
Patient Code . | Age y . | Sex . | Type of Pulmonary Specimen . | Date of Pulmonary Specimen Retrieval (in 2015) . | Underlying Condition . | Clinical Presentation of Pneumocystis Infection . | Prophylaxis . | CYB Alleleb . |
---|---|---|---|---|---|---|---|---|
HTRs | ||||||||
HTR1 | 44 | M | BAL | 17 March | Heart and renal transplantation; HIV infection | PCP | Atovaquone | CYB2 (C350T)c |
HTR2 | 35 | F | Induced sputum | 12 May | Heart transplantation | PCP | Atovaquone | CYB2 (C350T)c |
HTR3 | 68 | M | BAL | 15 July | Heart transplantation | PCP | None | CYB2 (C350T)c |
HTR4 | 64 | M | BAL | 28 July | Heart transplantation | PCP | Atovaquone | CYB2 (C350T)c |
HTR5 | 50 | M | BAL | 6 August | Heart transplantation | PCP | Atovaquone | CYB2 (C350T)c |
HTR6 | 69 | F | BAL | 24 August | Heart transplantation | PCP | Atovaquone | CYB2 (C350T)c |
HTR7 | 68 | F | Nasopharyngeal swab | 31 August | Heart transplantation | Colonization | None | CYB2 (C350T)c |
HTR8 | 38 | M | BAL | 2 September | Heart transplantation | PCP | Atovaquone | CYB2 (C350T)c |
HTR9 | 45 | M | Nasopharyngeal swab | 2 September | Heart transplantation | Colonization | Atovaquone | CYB2 (C350T)c |
Genotyping control patients | ||||||||
C1 | 69 | F | Induced sputum | 8 January | Corticosteroid treatment | Colonization | None | CYB1 |
C2 | 53 | M | BAL | 22 January | Liver transplantation | PCP | None | CYB2 |
C3 | 53 | M | BAL | 26 January | HIV infection | PCP | None | CYB1 |
C4 | 65 | M | BAL | 5 February | Corticosteroid treatment | PCP | None | CYB1 |
C5 | 70 | F | BAL | 24 February | Corticosteroid treatment | PCP | None | Mixed infectiond |
C6 | 44 | F | Induced sputum | 9 March | HIV infection | PCP | None | CYB1 |
C7 | 68 | M | BAL | 21 March | Liver transplantation | PCP | None | CYB2 |
C8 | 66 | M | BAL | 31 March | Cancer | PCP | None | Mixed infectione |
C9 | 64 | M | BAL | 13 April | Cancer | PCP | None | Mixed infectionf |
C10 | 72 | F | Sputum | 5 May | Cancer | PCP | Cotrimoxazole | CYB6 |
C11 | 56 | M | BAL | 7 May | Corticosteroid treatment | Colonization | None | Mixed infectione |
Patient Code . | Age y . | Sex . | Type of Pulmonary Specimen . | Date of Pulmonary Specimen Retrieval (in 2015) . | Underlying Condition . | Clinical Presentation of Pneumocystis Infection . | Prophylaxis . | CYB Alleleb . |
---|---|---|---|---|---|---|---|---|
HTRs | ||||||||
HTR1 | 44 | M | BAL | 17 March | Heart and renal transplantation; HIV infection | PCP | Atovaquone | CYB2 (C350T)c |
HTR2 | 35 | F | Induced sputum | 12 May | Heart transplantation | PCP | Atovaquone | CYB2 (C350T)c |
HTR3 | 68 | M | BAL | 15 July | Heart transplantation | PCP | None | CYB2 (C350T)c |
HTR4 | 64 | M | BAL | 28 July | Heart transplantation | PCP | Atovaquone | CYB2 (C350T)c |
HTR5 | 50 | M | BAL | 6 August | Heart transplantation | PCP | Atovaquone | CYB2 (C350T)c |
HTR6 | 69 | F | BAL | 24 August | Heart transplantation | PCP | Atovaquone | CYB2 (C350T)c |
HTR7 | 68 | F | Nasopharyngeal swab | 31 August | Heart transplantation | Colonization | None | CYB2 (C350T)c |
HTR8 | 38 | M | BAL | 2 September | Heart transplantation | PCP | Atovaquone | CYB2 (C350T)c |
HTR9 | 45 | M | Nasopharyngeal swab | 2 September | Heart transplantation | Colonization | Atovaquone | CYB2 (C350T)c |
Genotyping control patients | ||||||||
C1 | 69 | F | Induced sputum | 8 January | Corticosteroid treatment | Colonization | None | CYB1 |
C2 | 53 | M | BAL | 22 January | Liver transplantation | PCP | None | CYB2 |
C3 | 53 | M | BAL | 26 January | HIV infection | PCP | None | CYB1 |
C4 | 65 | M | BAL | 5 February | Corticosteroid treatment | PCP | None | CYB1 |
C5 | 70 | F | BAL | 24 February | Corticosteroid treatment | PCP | None | Mixed infectiond |
C6 | 44 | F | Induced sputum | 9 March | HIV infection | PCP | None | CYB1 |
C7 | 68 | M | BAL | 21 March | Liver transplantation | PCP | None | CYB2 |
C8 | 66 | M | BAL | 31 March | Cancer | PCP | None | Mixed infectione |
C9 | 64 | M | BAL | 13 April | Cancer | PCP | None | Mixed infectionf |
C10 | 72 | F | Sputum | 5 May | Cancer | PCP | Cotrimoxazole | CYB6 |
C11 | 56 | M | BAL | 7 May | Corticosteroid treatment | Colonization | None | Mixed infectione |
Abbreviations: BAL, bronchoalveolar lavage; CYB, cytochrome b (cyt b) gene; HIV, human immunodeficiency virus; HTRs, heart transplant recipients; PCP, Pneumocystis pneumonia.
aPatients were monitored at Bichat–Claude Bernard hospital, Paris, France, and P. jirovecii isolates were examined at the CYB gene.
bCytochrome b alleles were named using nomenclature described elsewhere by Esteves et al [15] and Maitte et al [16].
cCYB2 (C350T) allele corresponds to allele CYB2, as described elsewhere by Esteves et al [15], but it harbors a previously undescribed mutation on position 350 (C350T).
dPatient C5 presented with a mixed infection related to association of the alleles CYB1 and CYB7.
ePatients C8 and C11 presented with mixed infections. The alleles were not identified because polymorphisms were observed at 2 nucleotide positions; that is, 2 peaks were observed on chromatograms at 2 different positions.
fPatient C9 presented with a mixed infection related to association of the alleles CYB1 and CYB2.
Characteristics of 9 Heart Transplant Recipients and 11 Patients in the Genotyping Control Group Infected with Pneumocystis jirovecii a
Patient Code . | Age y . | Sex . | Type of Pulmonary Specimen . | Date of Pulmonary Specimen Retrieval (in 2015) . | Underlying Condition . | Clinical Presentation of Pneumocystis Infection . | Prophylaxis . | CYB Alleleb . |
---|---|---|---|---|---|---|---|---|
HTRs | ||||||||
HTR1 | 44 | M | BAL | 17 March | Heart and renal transplantation; HIV infection | PCP | Atovaquone | CYB2 (C350T)c |
HTR2 | 35 | F | Induced sputum | 12 May | Heart transplantation | PCP | Atovaquone | CYB2 (C350T)c |
HTR3 | 68 | M | BAL | 15 July | Heart transplantation | PCP | None | CYB2 (C350T)c |
HTR4 | 64 | M | BAL | 28 July | Heart transplantation | PCP | Atovaquone | CYB2 (C350T)c |
HTR5 | 50 | M | BAL | 6 August | Heart transplantation | PCP | Atovaquone | CYB2 (C350T)c |
HTR6 | 69 | F | BAL | 24 August | Heart transplantation | PCP | Atovaquone | CYB2 (C350T)c |
HTR7 | 68 | F | Nasopharyngeal swab | 31 August | Heart transplantation | Colonization | None | CYB2 (C350T)c |
HTR8 | 38 | M | BAL | 2 September | Heart transplantation | PCP | Atovaquone | CYB2 (C350T)c |
HTR9 | 45 | M | Nasopharyngeal swab | 2 September | Heart transplantation | Colonization | Atovaquone | CYB2 (C350T)c |
Genotyping control patients | ||||||||
C1 | 69 | F | Induced sputum | 8 January | Corticosteroid treatment | Colonization | None | CYB1 |
C2 | 53 | M | BAL | 22 January | Liver transplantation | PCP | None | CYB2 |
C3 | 53 | M | BAL | 26 January | HIV infection | PCP | None | CYB1 |
C4 | 65 | M | BAL | 5 February | Corticosteroid treatment | PCP | None | CYB1 |
C5 | 70 | F | BAL | 24 February | Corticosteroid treatment | PCP | None | Mixed infectiond |
C6 | 44 | F | Induced sputum | 9 March | HIV infection | PCP | None | CYB1 |
C7 | 68 | M | BAL | 21 March | Liver transplantation | PCP | None | CYB2 |
C8 | 66 | M | BAL | 31 March | Cancer | PCP | None | Mixed infectione |
C9 | 64 | M | BAL | 13 April | Cancer | PCP | None | Mixed infectionf |
C10 | 72 | F | Sputum | 5 May | Cancer | PCP | Cotrimoxazole | CYB6 |
C11 | 56 | M | BAL | 7 May | Corticosteroid treatment | Colonization | None | Mixed infectione |
Patient Code . | Age y . | Sex . | Type of Pulmonary Specimen . | Date of Pulmonary Specimen Retrieval (in 2015) . | Underlying Condition . | Clinical Presentation of Pneumocystis Infection . | Prophylaxis . | CYB Alleleb . |
---|---|---|---|---|---|---|---|---|
HTRs | ||||||||
HTR1 | 44 | M | BAL | 17 March | Heart and renal transplantation; HIV infection | PCP | Atovaquone | CYB2 (C350T)c |
HTR2 | 35 | F | Induced sputum | 12 May | Heart transplantation | PCP | Atovaquone | CYB2 (C350T)c |
HTR3 | 68 | M | BAL | 15 July | Heart transplantation | PCP | None | CYB2 (C350T)c |
HTR4 | 64 | M | BAL | 28 July | Heart transplantation | PCP | Atovaquone | CYB2 (C350T)c |
HTR5 | 50 | M | BAL | 6 August | Heart transplantation | PCP | Atovaquone | CYB2 (C350T)c |
HTR6 | 69 | F | BAL | 24 August | Heart transplantation | PCP | Atovaquone | CYB2 (C350T)c |
HTR7 | 68 | F | Nasopharyngeal swab | 31 August | Heart transplantation | Colonization | None | CYB2 (C350T)c |
HTR8 | 38 | M | BAL | 2 September | Heart transplantation | PCP | Atovaquone | CYB2 (C350T)c |
HTR9 | 45 | M | Nasopharyngeal swab | 2 September | Heart transplantation | Colonization | Atovaquone | CYB2 (C350T)c |
Genotyping control patients | ||||||||
C1 | 69 | F | Induced sputum | 8 January | Corticosteroid treatment | Colonization | None | CYB1 |
C2 | 53 | M | BAL | 22 January | Liver transplantation | PCP | None | CYB2 |
C3 | 53 | M | BAL | 26 January | HIV infection | PCP | None | CYB1 |
C4 | 65 | M | BAL | 5 February | Corticosteroid treatment | PCP | None | CYB1 |
C5 | 70 | F | BAL | 24 February | Corticosteroid treatment | PCP | None | Mixed infectiond |
C6 | 44 | F | Induced sputum | 9 March | HIV infection | PCP | None | CYB1 |
C7 | 68 | M | BAL | 21 March | Liver transplantation | PCP | None | CYB2 |
C8 | 66 | M | BAL | 31 March | Cancer | PCP | None | Mixed infectione |
C9 | 64 | M | BAL | 13 April | Cancer | PCP | None | Mixed infectionf |
C10 | 72 | F | Sputum | 5 May | Cancer | PCP | Cotrimoxazole | CYB6 |
C11 | 56 | M | BAL | 7 May | Corticosteroid treatment | Colonization | None | Mixed infectione |
Abbreviations: BAL, bronchoalveolar lavage; CYB, cytochrome b (cyt b) gene; HIV, human immunodeficiency virus; HTRs, heart transplant recipients; PCP, Pneumocystis pneumonia.
aPatients were monitored at Bichat–Claude Bernard hospital, Paris, France, and P. jirovecii isolates were examined at the CYB gene.
bCytochrome b alleles were named using nomenclature described elsewhere by Esteves et al [15] and Maitte et al [16].
cCYB2 (C350T) allele corresponds to allele CYB2, as described elsewhere by Esteves et al [15], but it harbors a previously undescribed mutation on position 350 (C350T).
dPatient C5 presented with a mixed infection related to association of the alleles CYB1 and CYB7.
ePatients C8 and C11 presented with mixed infections. The alleles were not identified because polymorphisms were observed at 2 nucleotide positions; that is, 2 peaks were observed on chromatograms at 2 different positions.
fPatient C9 presented with a mixed infection related to association of the alleles CYB1 and CYB2.
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