Table 2.
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DNA binding constants and specificities of PARP1 and PARP2 for nicked 1200-bp DNA fragment
PARP1PARP2 2
Kd (nM)SpecificityKd (nM)Specificity
AFM-site specific constants:
DNA nick5.88 ± 0.511349 ± 1343.7 ± 0.407297 ± 872
Non-specific7936 ± 47627027 ± 1270
Binding constants (AFM macro)a2.3 ± 0.153.0 ± 0.29
Binding constants (Flu)b0.84 ± 0.091.6 ± 0.12
PARP1PARP2 2
Kd (nM)SpecificityKd (nM)Specificity
AFM-site specific constants:
DNA nick5.88 ± 0.511349 ± 1343.7 ± 0.407297 ± 872
Non-specific7936 ± 47627027 ± 1270
Binding constants (AFM macro)a2.3 ± 0.153.0 ± 0.29
Binding constants (Flu)b0.84 ± 0.091.6 ± 0.12

aComposite macroscopic binding constants of PARP1 and PARP2 to nicked 1200-bp DNA fragments.

bMacroscopic binding constant of PARP1 and PARP2 to a 1200-bp DNA measured by fluorescence titration in this study (Figure 4E).

Table 2.
Open in new tab
DNA binding constants and specificities of PARP1 and PARP2 for nicked 1200-bp DNA fragment
PARP1PARP2 2
Kd (nM)SpecificityKd (nM)Specificity
AFM-site specific constants:
DNA nick5.88 ± 0.511349 ± 1343.7 ± 0.407297 ± 872
Non-specific7936 ± 47627027 ± 1270
Binding constants (AFM macro)a2.3 ± 0.153.0 ± 0.29
Binding constants (Flu)b0.84 ± 0.091.6 ± 0.12
PARP1PARP2 2
Kd (nM)SpecificityKd (nM)Specificity
AFM-site specific constants:
DNA nick5.88 ± 0.511349 ± 1343.7 ± 0.407297 ± 872
Non-specific7936 ± 47627027 ± 1270
Binding constants (AFM macro)a2.3 ± 0.153.0 ± 0.29
Binding constants (Flu)b0.84 ± 0.091.6 ± 0.12

aComposite macroscopic binding constants of PARP1 and PARP2 to nicked 1200-bp DNA fragments.

bMacroscopic binding constant of PARP1 and PARP2 to a 1200-bp DNA measured by fluorescence titration in this study (Figure 4E).

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